LOCUS       NP_064483               5085 aa            linear   ROD 13-FEB-2025
DEFINITION  protein piccolo isoform 1 [Rattus norvegicus].
ACCESSION   NP_064483
VERSION     NP_064483.2
DBSOURCE    REFSEQ: accession NM_020098.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 5085)
  AUTHORS   Falck,J., Bruns,C., Hoffmann-Conaway,S., Straub,I., Plautz,E.J.,
            Orlando,M., Munawar,H., Rivalan,M., Winter,Y., Izsvak,Z.,
            Schmitz,D., Hamra,F.K., Hallermann,S., Garner,C.C. and Ackermann,F.
  TITLE     Loss of Piccolo Function in Rats Induces Cerebellar Network
            Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes
  JOURNAL   J Neurosci 40 (14), 2943-2959 (2020)
   PUBMED   32122952
  REMARK    GeneRIF: Loss of Piccolo Function in Rats Induces Cerebellar
            Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like
            Phenotypes.
REFERENCE   2  (residues 1 to 5085)
  AUTHORS   Tao-Cheng,J.H.
  TITLE     Immunogold labeling of synaptic vesicle proteins in developing
            hippocampal neurons
  JOURNAL   Mol Brain 13 (1), 9 (2020)
   PUBMED   31959215
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 5085)
  AUTHORS   Ackermann,F., Schink,K.O., Bruns,C., Izsvak,Z., Hamra,F.K.,
            Rosenmund,C. and Garner,C.C.
  TITLE     Critical role for Piccolo in synaptic vesicle retrieval
  JOURNAL   Elife 8, e46629 (2019)
   PUBMED   31074746
  REMARK    GeneRIF: Piccolo is critical for the recycling and maintenance of
            synaptic vesicles.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 5085)
  AUTHORS   Parthier,D., Kuner,T. and Korber,C.
  TITLE     The presynaptic scaffolding protein Piccolo organizes the readily
            releasable pool at the calyx of Held
  JOURNAL   J Physiol 596 (8), 1485-1499 (2018)
   PUBMED   29194628
  REMARK    GeneRIF: Piccolo organizes the readily releasable pool of synaptic
            vesicles at the calyx of Held.
REFERENCE   5  (residues 1 to 5085)
  AUTHORS   Terry-Lorenzo,R.T., Torres,V.I., Wagh,D., Galaz,J., Swanson,S.K.,
            Florens,L., Washburn,M.P., Waites,C.L., Gundelfinger,E.D.,
            Reimer,R.J. and Garner,C.C.
  TITLE     Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone
            Proteins Piccolo and Bassoon
  JOURNAL   PLoS One 11 (12), e0167535 (2016)
   PUBMED   27907191
  REMARK    GeneRIF: The findings provide a mechanism for the presynaptic
            targeting of Trio and support a model in which Piccolo and Bassoon
            play a role in regulating neurotransmission through interactions
            with proteins, including Trio, that modulate the dynamic assembly
            of F-actin during cycles of synaptic vesicle exo- and endocytosis.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 5085)
  AUTHORS   Dick,O., Hack,I., Altrock,W.D., Garner,C.C., Gundelfinger,E.D. and
            Brandstatter,J.H.
  TITLE     Localization of the presynaptic cytomatrix protein Piccolo at
            ribbon and conventional synapses in the rat retina: comparison with
            Bassoon
  JOURNAL   J Comp Neurol 439 (2), 224-234 (2001)
   PUBMED   11596050
REFERENCE   7  (residues 1 to 5085)
  AUTHORS   Gerber,S.H., Garcia,J., Rizo,J. and Sudhof,T.C.
  TITLE     An unusual C(2)-domain in the active-zone protein piccolo:
            implications for Ca(2+) regulation of neurotransmitter release
  JOURNAL   EMBO J 20 (7), 1605-1619 (2001)
   PUBMED   11285225
REFERENCE   8  (residues 1 to 5085)
  AUTHORS   Zhai,R.G., Vardinon-Friedman,H., Cases-Langhoff,C., Becker,B.,
            Gundelfinger,E.D., Ziv,N.E. and Garner,C.C.
  TITLE     Assembling the presynaptic active zone: a characterization of an
            active one precursor vesicle
  JOURNAL   Neuron 29 (1), 131-143 (2001)
   PUBMED   11182086
REFERENCE   9  (residues 1 to 5085)
  AUTHORS   Fenster,S.D., Chung,W.J., Zhai,R., Cases-Langhoff,C., Voss,B.,
            Garner,A.M., Kaempf,U., Kindler,S., Gundelfinger,E.D. and
            Garner,C.C.
  TITLE     Piccolo, a presynaptic zinc finger protein structurally related to
            bassoon
  JOURNAL   Neuron 25 (1), 203-214 (2000)
   PUBMED   10707984
REFERENCE   10 (residues 1 to 5085)
  AUTHORS   Wang,X., Kibschull,M., Laue,M.M., Lichte,B., Petrasch-Parwez,E. and
            Kilimann,M.W.
  TITLE     Aczonin, a 550-kD putative scaffolding protein of presynaptic
            active zones, shares homology regions with Rim and Bassoon and
            binds profilin
  JOURNAL   J Cell Biol 147 (1), 151-162 (1999)
   PUBMED   10508862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Mar 2, 2021 this sequence version replaced NP_064483.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF227534.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q12"
     Protein         1..5085
                     /product="protein piccolo isoform 1"
                     /note="protein piccolo; presynaptic cytomatrix protein;
                     multidomain presynaptic cytomatrix protein Piccolo;
                     protein piccolo-like"
                     /calculated_mol_wt=552659
     Region          <64..516
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          174..517
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            212
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            232
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          <333..839
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          373..492
                     /region_name="12 X 10 AA tandem approximate repeats of
                     P-A-K-P-Q-P-Q-Q-P-X"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          522..583
                     /region_name="FYVE1_PCLO"
                     /note="FYVE-related domain 1 found in protein piccolo;
                     cd15774"
                     /db_xref="CDD:277313"
     Region          587..881
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          <661..1338
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Site            858
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            870
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            874
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          897..1013
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          1011..1072
                     /region_name="FYVE2_PCLO"
                     /note="FYVE-related domain 2 found in protein piccolo;
                     cd15776"
                     /db_xref="CDD:277315"
     Region          1070..1358
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          <1080..1637
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Site            1134
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1305
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1315
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1316
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1345
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1347
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1350
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1351
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1354
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          1374..1605
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1452
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1464
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1465
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1467
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1470
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1494
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1497
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1518
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1520
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1565
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1566
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1576
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1588
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          1623..1816
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1651
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1653
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1655
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1660
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1721
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1722
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1773
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1779
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1808
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1813
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1821
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            1842
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          2117..2140
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          2276..2386
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          2457..2487
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            2512
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            2702
                     /site_type="glycosylation"
                     /note="O-linked (GlcNAc) threonine. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            2976
                     /site_type="glycosylation"
                     /note="O-linked (GlcNAc) serine. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3014
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          3350..3457
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          3367..>3911
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Site            3374
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3388
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3392
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3419
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          3503..3572
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3522
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3530
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3561
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3565
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3571
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3574
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3577
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3598
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          3602..3695
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3624
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3626
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3632
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          3774..3816
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            3781
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4034
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4150
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4225..4248
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4272..4291
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4304
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4308
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4311
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4335..4357
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4340
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4376
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4438..4533
                     /region_name="PDZ_RIM-like"
                     /note="PDZ domain of Rab3-interacting molecule 1 (RIM),
                     RIM2, piccolo and related domains; cd06714"
                     /db_xref="CDD:467198"
     Site            order(4458..4464,4481,4484,4514,4518,4521..4522)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467198"
     Region          4589..4638
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Site            4609
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9QYX7; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4639..4771
                     /region_name="C2A_RIM1alpha"
                     /note="C2 domain first repeat contained in
                     Rab3-interacting molecule (RIM) proteins; cd04031"
                     /db_xref="CDD:175997"
     Site            order(4668,4674,4738,4740,4746)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175997"
     Site            4723
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4775..4851
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4874..4908
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JKS6.1)"
     Region          4968..5077
                     /region_name="C2"
                     /note="C2 domain; pfam00168"
                     /db_xref="CDD:425499"
     CDS             1..5085
                     /gene="Pclo"
                     /gene_synonym="aczonin"
                     /coded_by="NM_020098.2:236..15493"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="GeneID:56768"
                     /db_xref="RGD:69406"
ORIGIN      
        1 mgneaslege glpeglaaaa ggaggsgsal hpgipagmea dlsqlseeer rqiaavmsra
       61 qglpkgsvpa aaaespsmhr kqeldssqap qqpgkppdpg rptqpglsks rttdtfrseq
      121 klpgrspsti slkesksrtd fkeeykssmm pgffsdvnpl savssvvnkf npfdlisdse
      181 asqeettkkq kvvqkeqgks egmakpplqq pspkpipkqq gqvkeviqqd sspksvssqq
      241 aekvkpqapg tgkpsqqspa qtpaqqaspg kpvaqqpgsa katvqqpgpa kspaqpagtg
      301 kspaqppakt pgqqaglekt sssqqpgpks laqtpghgkf plgpvkspaq qpgtakhpaq
      361 qpgpqtaakv pgptktpaqq sgpgktpaqq pgptkpspqq pipakpqpqq pvatktqpqq
      421 sapakpqpqq papakpqpqq ptpakpqpqp ptpakpqpqp ptatkpqpqp ptatkphhqq
      481 pglakpsaqq ptksisqtvt grplqpppts aaqtpaqgls kticplcntt elllhipeka
      541 nfntctecqs tvcslcgfnp nphlteikew lclncqmqra lggdlaaaip sspqptpkaa
      601 taptatasks pvpsqqaspk keppskqdsp kaleskkppe pkkppepkkp pepkkppplv
      661 kqptlhgptp atapqlpvae alpepappke psgplpeqak apvgdvepkq pkmtetradi
      721 qsssttkpdi lssqvqsqaq vktasplktd sakpsqsfpp tgekttplds kamprpasds
      781 kiisqpgpgs eskdpkhidp iqkkdepkka qpkgspkpet kpvpkgsptp sgtrptagqa
      841 appsqqppkp qeqsrrfsln mggitdapks qpttpqetvt gklfgfgasi fsqasnlist
      901 agqqgphpqt gpaapskqap tpsqspaaqg pakstgqlpp apakatavkk eakaaaaenl
      961 eskpeqapta kktekdkkpp pakvgkppps epekavpahk pdkttkpkpa cplcrtelnl
     1021 gsqeppnfnt ctecknqvcn lcgfnptphl teiqewlcln cqtqraisgq lgdmgkmppa
     1081 psgpkaspmp apaepssqkt ptgtqvkgkk keaegkteae kpvpeketas iektppmvtt
     1141 dqkleesegk kskvsalpek kpseeekais adkkerkppa eekppleekk pipvdkklpp
     1201 eakplssege ekheilkahv qipeeeptgk vaaktgeeeq qpdsrpealp gatpltlpka
     1261 gekeravaqp qaegsskdgq gerskektek eedksdtsss qqpkspqgls dtgyssdgis
     1321 gslgeipsli psdekdllkg lkkdsfsqes spsspsdlak lestvlsile aqastlvgek
     1381 aekktqpqki spekpqdqqk tqtasetldi tiseeeikes qekkvspkkd seqgfpsrke
     1441 hkekpelvdd lsprrasyds vedssesens pvvrrkrrts igssssdeyk qedsqgsgee
     1501 edfirkqiie msadedasgs edeefirsql keisgvgesq kreeakgkgk gvagkhrrlt
     1561 rksstsfddd agrrhswhde ddetfdespe lkfretksqe seelvvaggg glrrfktiel
     1621 nstiadkyss essqkktily fdeepeleme sltdspedrs rgegssslha ssftpgtspt
     1681 svssldedsd sspshkkges kqqrkarhrs hgpllptied sseeeelree eellkeqekq
     1741 releqqqrks sskkskkdkd elraqrrrer pktppsnlsp iedaspteel rqaaemeelh
     1801 rsscseysps iesdpegfei spekiievqk vyklpaavsl ysptdeqsvm qkegvqkalk
     1861 saeemyeemm qkphkykafp aanerdevfe keplyggmli edyiyeslve dtyngsvdgs
     1921 lltrqeeqng fmqqrgreqk vrlqeqiydd pmqkisdlqk efyeleslhs vvpqedivss
     1981 syiipeshei vdlgsmvmst seekklldad sayeelmrrq qvqvtdgssp vqttigddma
     2041 estldfdrvq dasltssils gasltdstss atlsipdvki tqhfsaeele deyvtdytre
     2101 iqeiiahesl iltysepses atsvppsdtp sltssissvc ttdssspvtt ldslttvyte
     2161 padvmtkfkd seeisstyfp gsiidypedi svsldrtimp esrtnedqiv lsfsgmapsv
     2221 vesvgtkper pqadtistdl pisekdlikg kketgdgiil evldaykdkr eeseaeltki
     2281 slpepglaqa pssvtapqik eqhvsphsvs gkisgqekpt yrlpsgslpv sthpsksrpf
     2341 frsssldisa qppppppppp pppppppppp ppplppatsp kpptypkkkl avaatvtstt
     2401 ivtthvdalt tveaaaarrs nglpatkmca tapppvppkp sqiptglvft hrpeaikppi
     2461 apkpavpqip vttqkptdtc pkptglslts tmslnlvtsa dyklpsptsp lsphsnkssp
     2521 ryskslmdty vvitlpsepg tptdssaqai tswplgsppk dlvsletvfs vvppmtatei
     2581 psasqptlya sgalgtfsag pavtpslfqt vptpltqflp aevskpevsa vrstvpsvap
     2641 rsvsipippe plaldrhqyk engklplvgd aidlrtipkt eekatekcmd lsasavdvkr
     2701 qttvnevyrr qisavqpsii nlsaasslvt pitmdsktva vvtctdttvy ttgtesqggi
     2761 ehavtsplql ttskhtelpy rkpssqafpt irdeapinls lgpsaqavml avtkpvtvpp
     2821 vgvtngwtds tlsqgvadge vvdlstsksh rtvvtmdest snvvtkiied dekpvdltag
     2881 rravccdmvy tlpfgrscta qqpattlped rfgyrddhyq ydrsgpygyr giggmkpsms
     2941 dtnlaeaghf fyksknafdy sggtgaavdl tsgrvstgev mdysskttgp ypetrqvisg
     3001 vgistpqyst armtpppgpq ygvgsvlrss ngvvyssvat pipstfaitt qpgsifsttv
     3061 rdlsgipttd amtslsalhq sqpmprsyfi ttgasetdia vtgidinasl qtitmetlpa
     3121 eamdsvptlt tasevfsevv geestllivp dedkqqqqld lerellelek ikqqrfaeel
     3181 ewerqeiqrf reqekimvqk kleelqsmkq hllyqqeeer qaqfmmrqet laqqqlqleq
     3241 iqqlqqqlhq qleeqklrqi yqynydpsgt sspqttteqa ilegqyaate gsqfwateda
     3301 tttastvvai eipqsqgwyt vqsdgvtqyi appgilstvs eipltdvvvk eekqpkkrss
     3361 gakvrgqyde mgesvaddpr nlkkivdsgv qtddeetadr syasrrrrtk ksvdtsvqtd
     3421 dedqdewdmp srsrrkartg kygdstaegd ktkplskvss vavqtvaeis vqtepvgtir
     3481 tpsirarvda kveiikhisa pektykggsl gcqtetdsdt qsppylgats ppkdkkrptp
     3541 leigyssshl radptvqlap sppkspkvly spisplspgn alepafvpye kplpddispq
     3601 kvlhpdmakv ppaspktakm mqrsmsdpkp lsptadessr apfqysegft tkgsqtmtas
     3661 gtqkkvkrtl pnpppeevst gtqstystmg tasrrrmcrt ntmarakilq didreldlve
     3721 resaklrkkq aeldeeekei daklrylemg inrrkeallk erekrerayl qgvaedrdym
     3781 sdsevsstrp srvesqhgve rprtapqtef sqfippqtqt eaqlvpptsp ytqyqysspa
     3841 lptqaptpyt qqshfqqqtl yhqqvspyqt qptfqavatm sftpqaqptp tpqpsyqlps
     3901 qmmviqqkpr qttlylepki tsnyevirnq plmiapvstd ntyavshlgs kynsldlrig
     3961 leerssmass pissisadsf yadidhhtsr nyvliddigd itkgtaalst afslhekdls
     4021 ktdrllrtte trrsqevtdf laplqtssrl hsyvkaeedp medpyelkll khqikqefrr
     4081 gtesldhlag lshyyhadts yrhfpkseky sisrltlekq aakqlpaail yqkqskhkks
     4141 lidpkmskfs piqesrdlep dyptymssgt ssiggissra rllqdditfg lrknitdqqk
     4201 fmgsslgsgl gtlgntirsa lqdeadkpys sgsrsrpssr pssvygldls ikrdsssssl
     4261 rlkaqeaeal dvsfghssss artkptslpi sqsrgripiv aqnseeespl spvgqpmgma
     4321 raaagplppi sadtrdqfgs shslpevqqh mreesrtrgy drdiafimdd fqhamsdsea
     4381 yhlrreetdw fdkpresrle nghgldrklp erlvhsrpls qhqeqilqmn gktihyifph
     4441 arikitrdsk dhtvsgnglg irivggkeip ghsgeigayi akilpggsae htgklmegmq
     4501 vlewngvplt sktyeevqsi inqqsgeaei cvrldlnmls dsenpqhlel heppkvdkak
     4561 spgvdpkqla aelqkvslqq splvmssvve kgshahsgpt sagsssvpsp gqpgspsvsk
     4621 kkhsstkptd atkaashpit geiqlqinyd lgnliihilq arnlvprdnn gysdpfvkvy
     4681 llpgrgqvmv vqnasaeykr rtkyvqksln pewnqtviyk sismeqlmkk tlevtvwdyd
     4741 rfssndflge vlidlsstsh ldntprwypl keqtesidhg kshssqnsqq spkpsviksr
     4801 shgifpdpsk dmqvptieks hsspgsskss seghlrshgp srsqsktsva qthledagva
     4861 iaaaeaavqq lriqptkptn hrpaessvst gssgssvgsg ysvdsegssc vagepnllpi
     4921 prigkmgqng qdpvkqpgmg atdtegktqv mgeiklalkk emktdgeqli veilqcrnit
     4981 ykfkspdhlp dlyvklyvin istqkkvikk ktrvcrhdre psfnetfrfs lspaghslqi
     5041 llfsnggkfm kktligeaci wldkvdlrkr ivnwhkllvs ptqth
//
