LOCUS       NM_001108377            2203 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus phosphoenolpyruvate carboxykinase 2
            (mitochondrial) (Pck2), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001108377 XM_001055522 XM_341319
VERSION     NM_001108377.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Stark,R., Guebre-Egziabher,F., Zhao,X., Feriod,C., Dong,J.,
            Alves,T.C., Ioja,S., Pongratz,R.L., Bhanot,S., Roden,M.,
            Cline,G.W., Shulman,G.I. and Kibbey,R.G.
  TITLE     A role for mitochondrial phosphoenolpyruvate carboxykinase
            (PEPCK-M) in the regulation of hepatic gluconeogenesis
  JOURNAL   J Biol Chem 289 (11), 7257-7263 (2014)
   PUBMED   24497630
  REMARK    GeneRIF: PEPCK-M has a direct role in fasted and fed glucose
            homeostasis
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Stark,R., Pasquel,F., Turcu,A., Pongratz,R.L., Roden,M.,
            Cline,G.W., Shulman,G.I. and Kibbey,R.G.
  TITLE     Phosphoenolpyruvate cycling via mitochondrial phosphoenolpyruvate
            carboxykinase links anaplerosis and mitochondrial GTP with insulin
            secretion
  JOURNAL   J Biol Chem 284 (39), 26578-26590 (2009)
   PUBMED   19635791
  REMARK    GeneRIF: Data show that PEPCK-M message and protein were detected
            in INS-1 832/13 cells and islets.
REFERENCE   3  (bases 1 to 2203)
  AUTHORS   Caton,P.W., Nayuni,N.K., Murch,O. and Corder,R.
  TITLE     Endotoxin induced hyperlactatemia and hypoglycemia is linked to
            decreased mitochondrial phosphoenolpyruvate carboxykinase
  JOURNAL   Life Sci 84 (21-22), 738-744 (2009)
   PUBMED   19268478
REFERENCE   4  (bases 1 to 2203)
  AUTHORS   Jitrapakdee,S., St Maurice,M., Rayment,I., Cleland,W.W.,
            Wallace,J.C. and Attwood,P.V.
  TITLE     Structure, mechanism and regulation of pyruvate carboxylase
  JOURNAL   Biochem J 413 (3), 369-387 (2008)
   PUBMED   18613815
  REMARK    Review article
REFERENCE   5  (bases 1 to 2203)
  AUTHORS   Nyirenda,M.J., Dean,S., Lyons,V., Chapman,K.E. and Seckl,J.R.
  TITLE     Prenatal programming of hepatocyte nuclear factor 4alpha in the
            rat: A key mechanism in the 'foetal origins of hyperglycaemia'?
  JOURNAL   Diabetologia 49 (6), 1412-1420 (2006)
   PUBMED   16570165
REFERENCE   6  (bases 1 to 2203)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2203)
  AUTHORS   Song,S., Andrikopoulos,S., Filippis,C., Thorburn,A.W., Khan,D. and
            Proietto,J.
  TITLE     Mechanism of fat-induced hepatic gluconeogenesis: effect of
            metformin
  JOURNAL   Am J Physiol Endocrinol Metab 281 (2), E275-E282 (2001)
   PUBMED   11440903
REFERENCE   8  (bases 1 to 2203)
  AUTHORS   Berry,M.N., Gregory,R.B., Grivell,A.R., Henly,D.C., Phillips,J.W.,
            Wallace,P.G. and Welch,G.R.
  TITLE     Evidence that stimulation of gluconeogenesis by fatty acid is
            mediated through thermodynamic mechanisms
  JOURNAL   FEBS Lett 231 (1), 19-24 (1988)
   PUBMED   2966075
REFERENCE   9  (bases 1 to 2203)
  AUTHORS   Wimhurst,J.M. and Manchester,K.L.
  TITLE     Induction and suppression of the key enzymes of glycolysis and
            gluconeogenesis in isolated perfused rat liver in response to
            glucose, fructose and lactate
  JOURNAL   Biochem J 134 (1), 143-156 (1973)
   PUBMED   4353083
REFERENCE   10 (bases 1 to 2203)
  AUTHORS   Ballard,F.J. and Hanson,R.W.
  TITLE     Phosphoenolpyruvate carboxykinase and pyruvate carboxylase in
            developing rat liver
  JOURNAL   Biochem J 104 (3), 866-871 (1967)
   PUBMED   6049928
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB787746.1 and BC166766.1.
            
            On May 29, 2008 this sequence version replaced NM_001108377.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                CB787746.1         54-80
            28-2203             BC166766.1         1-2176
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p13"
     gene            1..2203
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /note="phosphoenolpyruvate carboxykinase 2
                     (mitochondrial)"
                     /db_xref="GeneID:361042"
                     /db_xref="RGD:1311112"
     exon            1..127
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..2021
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /codon_start=1
                     /product="phosphoenolpyruvate carboxykinase [GTP],
                     mitochondrial"
                     /protein_id="NP_001101847.2"
                     /db_xref="GeneID:361042"
                     /db_xref="RGD:1311112"
                     /translation="MAAMYLPGLRLSWHRLRPWCRSPCRSIQTLRVLSGDLSQLPAGV
                     RDFVERSAHLCQPAGIHICDGTEAENAATLALLEEQGLIRKLPKYENCWLARTDPKDV
                     ARVESKTVIVTSSQRDTVPLLAGGASGQLGNWMSPDEFQRAVDQRFPGCMQGRTMYVL
                     PFSMGPLGSPLSRIGVQLTDSPYVVASMRIMTRLGTHVLQALGDGDFIKCLHSVGQPL
                     TGHGDPVGRWPCNPEKTLIGHVPEQREIVSFGSGYGGNSLLGKKCFALRIASRLARDE
                     GWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMR
                     FDSEGQLRAINPENGFFGVAPGTSATTNPNAMATIQSNTVFTNVAETSDGGVYWEGID
                     QPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCVPARQCPIMDPAWEAPEGVPIDAII
                     FGGRRPKGVPLVYEAFSWRHGVFVGSAMRSESTAAAEHKGKTIMHDPFAMRPFFGYNF
                     GHYLEHWLSMEGRKGARLPRIFHVNWFRRDEAGRFLWPGFGENARVLDWICRRLGGED
                     SARETPIGLVPKEGALDLSGLRAIDTSQLFSIPKDFWEQEVRDIRSYLTEQVNQDLPK
                     EVLAELEALEERVQKM"
     exon            128..373
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            374..558
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            559..762
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            763..950
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1113
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1332
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1470
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1566
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..2185
                     /gene="Pck2"
                     /gene_synonym="PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctccctctgg ctttgccacg cttcttcctt ccccgccttc cttgactccc cgactgcact
       61 ggttcctgtg gccatcccgc agcgccagtc caggtgccat ggctgctatg tacctccccg
      121 gcctgcggct tagctggcac aggctgaggc cctggtgccg gtcaccatgc cgtagcatcc
      181 aaaccctgcg cgtgctcagt ggagatctga gccagctgcc ggctggggtt cgagactttg
      241 tggagcgcag tgcccatctg tgccaaccag caggcatcca tatttgtgat gggactgagg
      301 ctgagaacgc tgccacgctg gccctgctgg aagagcaggg tcttatccgg aagctcccca
      361 agtatgagaa ctgctggctg gcccgcacag accccaagga tgtggcacgg gtagaaagca
      421 agacggtgat tgtaacttct tcgcagcggg acacagtgcc tctcctggct ggtggggcca
      481 gtgggcagct gggcaactgg atgtccccag atgagttcca gagagctgtg gaccagagat
      541 tcccaggatg catgcagggc cgcaccatgt atgtgcttcc gttcagcatg ggtcccttgg
      601 gctccccgct ctcccgcatt ggagtgcagc tcactgactc gccttatgta gtggcaagca
      661 tgcggattat gacccgcctg gggacacatg tactccaggc cctgggagat ggtgacttca
      721 tcaagtgtct gcattcggtg ggccagcccc tgactggaca tggggatcct gtgggccggt
      781 ggccatgcaa tccggaaaaa accctgattg gccacgtgcc ggagcagcgg gagatcgtct
      841 ccttcggcag cggctatggt gggaactcct tgctgggcaa gaagtgtttt gccctgcgca
      901 tcgcctctcg cctggccagg gatgagggct ggctggcaga acacatgctg attttgggca
      961 tcaccaaccc cgcagggaaa aagcgctatg tggcagctgc tttccccagt gcctgtggca
     1021 agaccaatct ggccatgatg cggcctgctt tgccaggctg gaaagtggag tgtgtggggg
     1081 atgacatcgc ctggatgagg tttgacagtg aaggtcaact ccgggccatc aaccctgaga
     1141 atggcttctt cggggtggcc cctggtacct ctgccaccac caatcccaat gccatggcca
     1201 caatccagag taacactgtc ttcaccaatg tggctgagac cagtgatggc ggtgtgtact
     1261 gggaaggcat tgaccagcct cttccacctg gtgtcaccgt aacctcctgg ctgggaaagc
     1321 catggaaacc tggggacaag gaaccctgtg cgcatccaaa ctctcgcttt tgtgtcccgg
     1381 ctcgccagtg ccccatcatg gacccagcct gggaggcacc agaaggtgtt ccaattgatg
     1441 ccatcatctt cggaggccgc agacctaaag gggtaccact ggtgtatgag gccttcagct
     1501 ggcgccatgg ggtgtttgtc ggtagtgcca tgcgctctga gtccactgca gctgctgaac
     1561 acaagggaaa gaccattatg cacgatccct ttgccatgcg accttttttt ggctataact
     1621 tcggacacta cctggaacac tggttgagca tggagggacg aaaaggtgcc cggctgcctc
     1681 gtatcttcca tgtcaactgg ttccggagag atgaagcagg ccgcttcctg tggccaggct
     1741 ttggggaaaa cgctcgcgtg ctagactgga tctgccgaag attaggagga gaagacagtg
     1801 cccgagagac tcccattggg ctcgtaccaa aggaaggagc cctggatctc agtggcctcc
     1861 gagcaataga taccagtcag ctgttctcca tccccaagga cttctgggaa caggaggttc
     1921 gtgatattag gagctacctg acagagcaag tcaaccagga tctgcccaag gaggtgttgg
     1981 ctgagctcga ggccctggaa gagcgcgtgc aaaaaatgtg acctgaggcc ttaggctagc
     2041 aagagcgcag cacccccatc tgggtagagg atcctaaggc tcacagaaaa cgtgaacaat
     2101 ttgacattaa aatgtgtgag tgttgggagg ccaagccaca gacctcctcc catgctgtcc
     2161 gataagagat gcacactttg ctacaaaaaa aaaaaaaaaa aaa
//
