LOCUS       NM_198780               2644 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus phosphoenolpyruvate carboxykinase 1 (Pck1), mRNA.
ACCESSION   NM_198780 XM_342593
VERSION     NM_198780.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Elnagar,A., El-Dawy,K., El-Belbasi,H.I., Rehan,I.F., Embark,H.,
            Al-Amgad,Z., Shanab,O., Mickdam,E., Batiha,G.E., Alamery,S.,
            Fouad,S.S., Cavalu,S. and Youssef,M.
  TITLE     Ameliorative Effect of Oxytocin on FBN1 and PEPCK Gene Expression,
            and Behavioral Patterns in Rats' Obesity-Induced Diabetes
  JOURNAL   Front Public Health 10, 777129 (2022)
   PUBMED   35462799
  REMARK    GeneRIF: Ameliorative Effect of Oxytocin on FBN1 and PEPCK Gene
            Expression, and Behavioral Patterns in Rats' Obesity-Induced
            Diabetes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Coelho,N.R., Tomkiewicz,C., Correia,M.J., Goncalves-Dias,C.,
            Barouki,R., Pereira,S.A., Coumoul,X. and Monteiro,E.C.
  TITLE     First evidence of aryl hydrocarbon receptor as a druggable target
            in hypertension induced by chronic intermittent hypoxia
  JOURNAL   Pharmacol Res 159, 104869 (2020)
   PUBMED   32416216
REFERENCE   3  (bases 1 to 2644)
  AUTHORS   Sharma,R., Kumari,M., Prakash,P., Gupta,S. and Tiwari,S.
  TITLE     Phosphoenolpyruvate carboxykinase in urine exosomes reflect
            impairment in renal gluconeogenesis in early insulin resistance and
            diabetes
  JOURNAL   Am J Physiol Renal Physiol 318 (3), F720-F731 (2020)
   PUBMED   32036699
  REMARK    GeneRIF: The relationship between PEPCK metabolism in the urinary
            tract and insulin resistance and diabetes is reported.
REFERENCE   4  (bases 1 to 2644)
  AUTHORS   Westermeier,F., Holyoak,T., Gatica,R., Martinez,F., Negron,M.,
            Yanez,A.J., Nahmias,D., Nualart,F., Burbulis,I. and Bertinat,R.
  TITLE     Cytosolic phosphoenolpyruvate carboxykinase is expressed in
            alpha-cells from human and murine pancreas
  JOURNAL   J Cell Physiol 235 (1), 166-175 (2020)
   PUBMED   31180589
  REMARK    GeneRIF: Cytosolic phosphoenolpyruvate carboxykinase is expressed
            in alpha-cells from human and murine pancreas.
REFERENCE   5  (bases 1 to 2644)
  AUTHORS   Mcleod,M.J., Krismanich,A.P., Assoud,A., Dmitrienko,G.I. and
            Holyoak,T.
  TITLE     Characterization of 3-[(Carboxymethyl)thio]picolinic Acid: A Novel
            Inhibitor of Phosphoenolpyruvate Carboxykinase
  JOURNAL   Biochemistry 58 (37), 3918-3926 (2019)
   PUBMED   31461616
REFERENCE   6  (bases 1 to 2644)
  AUTHORS   Miller,W.H., Daluge,S.M., Garvey,E.P., Hopkins,S., Reardon,J.E.,
            Boyd,F.L. and Miller,R.L.
  TITLE     Phosphorylation of carbovir enantiomers by cellular enzymes
            determines the stereoselectivity of antiviral activity
  JOURNAL   J Biol Chem 267 (29), 21220-21224 (1992)
   PUBMED   1383219
REFERENCE   7  (bases 1 to 2644)
  AUTHORS   Faber,S., Ip,T., Granner,D. and Chalkley,R.
  TITLE     The interplay of ubiquitous DNA-binding factors, availability of
            binding sites in the chromatin, and DNA methylation in the
            differential regulation of phosphoenolpyruvate carboxykinase gene
            expression
  JOURNAL   Nucleic Acids Res 19 (17), 4681-4688 (1991)
   PUBMED   1716357
REFERENCE   8  (bases 1 to 2644)
  AUTHORS   Ip,Y.T., Poon,D., Stone,D., Granner,D.K. and Chalkley,R.
  TITLE     Interaction of a liver-specific factor with an enhancer 4.8
            kilobases upstream of the phosphoenolpyruvate carboxykinase gene
  JOURNAL   Mol Cell Biol 10 (7), 3770-3781 (1990)
   PUBMED   2355922
REFERENCE   9  (bases 1 to 2644)
  AUTHORS   Lewis,C.T., Seyer,J.M. and Carlson,G.M.
  TITLE     Cysteine 288: an essential hyperreactive thiol of cytosolic
            phosphoenolpyruvate carboxykinase (GTP)
  JOURNAL   J Biol Chem 264 (1), 27-33 (1989)
   PUBMED   2909519
REFERENCE   10 (bases 1 to 2644)
  AUTHORS   Iynedjian,P.B., Auberger,P., Guigoz,Y. and Le Cam,A.
  TITLE     Pretranslational regulation of tyrosine aminotransferase and
            phosphoenolpyruvate carboxykinase (GTP) synthesis by glucagon and
            dexamethasone in adult rat hepatocytes
  JOURNAL   Biochem J 225 (1), 77-84 (1985)
   PUBMED   2858199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1 and BC081900.1.
            
            On Mar 25, 2005 this sequence version replaced NM_198780.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081900.1, SRR26643290.14413.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760475
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                JAXUCZ010000003.1  182348572-182348598
            28-2644             BC081900.1         1-2617
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..2644
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /note="phosphoenolpyruvate carboxykinase 1"
                     /db_xref="GeneID:362282"
                     /db_xref="RGD:3267"
     exon            1..102
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            103..367
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..2012
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /EC_number="4.1.1.32"
                     /note="phosphoenolpyruvate carboxykinase, cytosolic (GTP);
                     phosphoenolpyruvate carboxylase; phosphoenolpyruvate
                     carboxykinase 1, cytosolic; phosphoenolpyruvate
                     carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1
                     (soluble); serine-protein kinase PCK1"
                     /codon_start=1
                     /product="phosphoenolpyruvate carboxykinase, cytosolic
                     [GTP]"
                     /protein_id="NP_942075.1"
                     /db_xref="GeneID:362282"
                     /db_xref="RGD:3267"
                     /translation="MPPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIH
                     ICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDT
                     VPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIEL
                     TDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTL
                     IAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGK
                     KKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFG
                     VAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWR
                     PQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSW
                     QHGVFVGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKL
                     PKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNL
                     KGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM"
     exon            368..549
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            550..753
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            754..941
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1104
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1329
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1461
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1557
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..2635
                     /gene="Pck1"
                     /gene_synonym="GTP; PCK; Pepck; PEPCK-C; RATPEPCK"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acggttggcc ttccctctgg gaacacaccc ttggccaaca ggggaaatcc ggcgagacgc
       61 tctgagatct ctgatccaga ccttccaaaa ggaggaagaa aggtggcacc agagtccctg
      121 cctctctcca caccattgca agaatgcctc ctcagctgca taatggtctg gacttctctg
      181 ccaaggtcat ccagggcagc ctcgacagcc tgccccagga agtgaggaag tttgtggaag
      241 gcaatgccca gctgtgccag ccagagtata ttcacatctg cgacggctcc gaggaggagt
      301 acgggcggct gctggcccac atgcaggagg agggtgtcat ccgcaagctg aagaaatatg
      361 acaactgttg gctggctctc actgacccca gggatgtggc caggatcgaa agcaagacgg
      421 tcatcattac ccaagagcag agagacaccg tgcccatccc caaaagtggg cagagccagc
      481 tgggccgctg gatgtcagaa gaggacttcg agaaagcatt caacgccagg ttcccggggt
      541 gcatgaaagg ccgcaccatg tatgtcatcc cattcagcat ggggccgctg ggctcacctc
      601 tggccaagat tggtattgag ctgacagact cgccctatgt ggtggccagc atgcggatca
      661 tgacacggat ggggacctct gtgctggagg ccctgggcga tggggagttt atcaagtgcc
      721 tccactccgt ggggtgccct ctccccttaa aaaagccttt ggtcaacaac tgggcctgca
      781 accccgagct gaccctgatt gctcacctcc cggaccgcag agagatcatc tccttcggaa
      841 gcggatacgg tgggaactca ctgcttggga agaaatgctt tgcgctgcgg atcgccagca
      901 ggctggctaa ggaggaaggg tggctggcgg agcacatgct gatcctgggc ataactaacc
      961 ccgaaggcaa gaagaaatac ctggcagcag ccttccccag tgcctgtggg aaaaccaacc
     1021 tggccatgat gaaccccacc ctccccgggt ggaaagttga atgtgtgggt gatgacattg
     1081 cctggatgaa gtttgatgcc caaggcaact taagggccat caacccagaa aacggttttt
     1141 ttggagttgc tccgggcacc tcagtgaaga caaatccgaa cgccattaag accatccaga
     1201 aaaacaccat cttcaccaac gtggctgaga caagtgatgg gggtgtttac tgggaaggca
     1261 tcgatgagcc cctggcccca ggagtcacca tcacttcctg gaagaacaaa gagtggagac
     1321 cacaggatga ggaaccgtgc gcccatccca actcgcgatt ctgcacccct gccagccaat
     1381 gtcccattat tgaccccgcc tgggaatctc ctgaaggagt gcccatcgaa ggcatcattt
     1441 ttggtggccg tagacctgca ggtgtccccc ttgtctacga agctctcagc tggcagcatg
     1501 gggtgtttgt aggagctgcc atgagatcag aggccaccgc tgctgcagag cataagggca
     1561 aggtcatcat gcacgacccc ttcgctatgc ggcccttctt tggctacaac ttcggcaagt
     1621 acctggcgca ctggctgagc atggcccacc gcccagcagc caagttgccc aagatcttcc
     1681 acgtcaactg gttccggaaa gacaaaaacg gcaagttcct ctggcccgga tttggtgaga
     1741 actcccgcgt gctggagtgg atgttcggac gcatcgaagg ggaagacagc gccaagctca
     1801 ctcccattgg ctacgtccct aaggaagacg ccctgaactt gaaaggcctg ggggacgtca
     1861 acgtggagga gctgttcgga atctctaagg aattctggga gaaggaggtg gaggagatcg
     1921 acaagtatct ggaggaccag gtcaacgccg acctccctta cgaaatagag agggagctcc
     1981 gagccctgaa acagagaatc agccagatgt aatcccgatg gggggtgtcc ttgagagtcg
     2041 ccccttcccg ctcacggcac gcgttgggag ctaggagcaa accagcaagc acaatgctga
     2101 gtagatcaga aaagcacctt ttaatagtca gttgagtagc acagagaaca ggctagggac
     2161 aaataagatt gggaggggaa atcacgtctt gttctccgaa gttcgcatct gacactgatg
     2221 gggggtttgg ttccacttcg aggtcactca ggaatccagt tcttcacgtt agctgtagca
     2281 gttagctaaa gtgcacagaa aacatacttg agctgtatat atgtgtgtga gcatgtgtgt
     2341 gtgagcgtgt gcatgcgtgc gtgcgtgcgt gcgcgtgtgt gtgtgtgtgt gtacatgtgt
     2401 acatgtgtac atgcctgtct gtctcattgt ctccaatgta tttaaaacct ttggggagaa
     2461 aaaaaatctt gggcaagttt gtagttataa ctagagagtc gtgttgcttt gttgctagta
     2521 tgtatgttta aattattttt atacactgcc ctttcttacc tttctttaca taattgaaat
     2581 aggtatcctg accacttctt ggaaaaaaaa taaactttta tagaaaaaaa atggaaaaaa
     2641 aaaa
//
