LOCUS       NM_001401319           12502 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Rattus norvegicus pappalysin (Pappa), mRNA.
ACCESSION   NM_001401319 XM_006238269
VERSION     NM_001401319.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Dya,G.A., Lebedeva,O.S., Gushchevarov,D.A., Volovikov,E.A.,
            Belikova,L.D., Kopylova,I.V., Postnikov,A.B., Artemieva,M.M.,
            Medvedeva,N.A., Lagarkova,M.A., Katrukha,A.G. and Serebryanaya,D.V.
  TITLE     Specific cleavage of IGFBP-4 by papp-a in nervous tissue
  JOURNAL   Biochem Biophys Res Commun 733, 150655 (2024)
   PUBMED   39244846
  REMARK    GeneRIF: Specific cleavage of IGFBP-4 by papp-a in nervous tissue.
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Serebryanaya,D.V., Adasheva,D.A., Konev,A.A., Artemieva,M.M.,
            Katrukha,I.A., Postnikov,A.B., Medvedeva,N.A. and Katrukha,A.G.
  TITLE     IGFBP-4 Proteolysis by PAPP-A in a Primary Culture of Rat Neonatal
            Cardiomyocytes under Normal and Hypertrophic Conditions
  JOURNAL   Biochemistry (Mosc) 86 (11), 1395-1406 (2021)
   PUBMED   34906040
  REMARK    GeneRIF: IGFBP-4 Proteolysis by PAPP-A in a Primary Culture of Rat
            Neonatal Cardiomyocytes under Normal and Hypertrophic Conditions.
REFERENCE   3  (bases 1 to 12502)
  AUTHORS   Henning,A.N., Haag,J.D., Smits,B.M. and Gould,M.N.
  TITLE     The Non-coding Mammary Carcinoma Susceptibility Locus, Mcs5c,
            Regulates Pappa Expression via Age-Specific Chromatin Folding and
            Allele-Dependent DNA Methylation
  JOURNAL   PLoS Genet 12 (8), e1006261 (2016)
   PUBMED   27537370
  REMARK    GeneRIF: Pappa has been shown to positively regulate the IGF
            signaling pathway, which is required for proper mammary
            gland/breast development and is of increasing interest in breast
            cancer pathogenesis. Mcs5c-mediated regulation of Pappa appears to
            occur through age-dependent and mammary gland-specific chromatin
            looping, as well as genotype-dependent CpG island shore methylation
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12502)
  AUTHORS   Abonnenc,M., Nabeebaccus,A.A., Mayr,U., Barallobre-Barreiro,J.,
            Dong,X., Cuello,F., Sur,S., Drozdov,I., Langley,S.R., Lu,R.,
            Stathopoulou,K., Didangelos,A., Yin,X., Zimmermann,W.H., Shah,A.M.,
            Zampetaki,A. and Mayr,M.
  TITLE     Extracellular matrix secretion by cardiac fibroblasts: role of
            microRNA-29b and microRNA-30c
  JOURNAL   Circ Res 113 (10), 1138-1147 (2013)
   PUBMED   24006456
REFERENCE   5  (bases 1 to 12502)
  AUTHORS   Bale,L.K., Resch,Z.T., Harstad,S.L., Overgaard,M.T. and
            Conover,C.A.
  TITLE     Constitutive expression of pregnancy-associated plasma protein-A in
            arterial smooth muscle reduces the vascular response to injury in
            vivo
  JOURNAL   Am J Physiol Endocrinol Metab 304 (2), E139-E144 (2013)
   PUBMED   23169786
  REMARK    GeneRIF: Differential effects of chronic (transgenic) and transient
            (endogenous) PAPP-A expression on neointimal formation following
            vascular injury that may be due in part to the differential impact
            on IGF-I signaling.
REFERENCE   6  (bases 1 to 12502)
  AUTHORS   Soe,R., Overgaard,M.T., Thomsen,A.R., Laursen,L.S., Olsen,I.M.,
            Sottrup-Jensen,L., Haaning,J., Giudice,L.C., Conover,C.A. and
            Oxvig,C.
  TITLE     Expression of recombinant murine pregnancy-associated plasma
            protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential
            proteolytic activity
  JOURNAL   Eur J Biochem 269 (8), 2247-2256 (2002)
   PUBMED   11985604
REFERENCE   7  (bases 1 to 12502)
  AUTHORS   Boldt,H.B., Overgaard,M.T., Laursen,L.S., Weyer,K.,
            Sottrup-Jensen,L. and Oxvig,C.
  TITLE     Mutational analysis of the proteolytic domain of
            pregnancy-associated plasma protein-A (PAPP-A): classification as a
            metzincin
  JOURNAL   Biochem J 358 (Pt 2), 359-367 (2001)
   PUBMED   11513734
REFERENCE   8  (bases 1 to 12502)
  AUTHORS   Lawrence,J.B., Oxvig,C., Overgaard,M.T., Sottrup-Jensen,L.,
            Gleich,G.J., Hays,L.G., Yates,J.R. 3rd and Conover,C.A.
  TITLE     The insulin-like growth factor (IGF)-dependent IGF binding
            protein-4 protease secreted by human fibroblasts is
            pregnancy-associated plasma protein-A
  JOURNAL   Proc Natl Acad Sci U S A 96 (6), 3149-3153 (1999)
   PUBMED   10077652
REFERENCE   9  (bases 1 to 12502)
  AUTHORS   Pedersen,J.F., Sorensen,S. and Molsted-Pedersen,L.
  TITLE     Serum levels of human placental lactogen, pregnancy-associated
            plasma protein A and endometrial secretory protein PP14 in first
            trimester of diabetic pregnancy
  JOURNAL   Acta Obstet Gynecol Scand 77 (2), 155-158 (1998)
   PUBMED   9512318
REFERENCE   10 (bases 1 to 12502)
  AUTHORS   Waites,G.T., Thomson,A.W., Sewell,H.F. and Bell,S.C.
  TITLE     Detection and immunohistochemical localization of alpha
            1-pregnancy-associated protein (alpha 1-PAP) in rats
  JOURNAL   Immunology 58 (2), 303-309 (1986)
   PUBMED   2423447
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Feb 8, 2022 this sequence version replaced XM_006238269.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.13411.1,
                                           SRR21887623.2848058.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN11044071, SAMN12840112
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1195              JAXUCZ010000005.1  83513358-83514552
            1196-2258           JAXUCZ010000005.1  83547378-83548440
            2259-2404           JAXUCZ010000005.1  83567204-83567349
            2405-2698           JAXUCZ010000005.1  83571723-83572016
            2699-2891           JAXUCZ010000005.1  83579290-83579482
            2892-3013           JAXUCZ010000005.1  83586485-83586606
            3014-3512           JAXUCZ010000005.1  83594252-83594750
            3513-3641           JAXUCZ010000005.1  83618281-83618409
            3642-3733           JAXUCZ010000005.1  83625780-83625871
            3734-3927           JAXUCZ010000005.1  83649273-83649466
            3928-4041           JAXUCZ010000005.1  83684492-83684605
            4042-4177           JAXUCZ010000005.1  83685539-83685674
            4178-4391           JAXUCZ010000005.1  83688687-83688900
            4392-4560           JAXUCZ010000005.1  83696856-83697024
            4561-4744           JAXUCZ010000005.1  83699429-83699612
            4745-4955           JAXUCZ010000005.1  83707394-83707604
            4956-5127           JAXUCZ010000005.1  83708639-83708810
            5128-5275           JAXUCZ010000005.1  83716126-83716273
            5276-5385           JAXUCZ010000005.1  83719613-83719722
            5386-5457           JAXUCZ010000005.1  83728107-83728178
            5458-5556           JAXUCZ010000005.1  83733910-83734008
            5557-12502          JAXUCZ010000005.1  83744416-83751361
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q24"
     gene            1..12502
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /note="pappalysin"
                     /db_xref="GeneID:313262"
                     /db_xref="RGD:1307742"
     exon            1..1195
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     CDS             790..5664
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /EC_number="3.4.24.79"
                     /note="pregnancy-associated plasma protein A; pappalysin
                     1"
                     /codon_start=1
                     /product="pappalysin-1 precursor"
                     /protein_id="NP_001388248.1"
                     /db_xref="GeneID:313262"
                     /db_xref="RGD:1307742"
                     /translation="MRLWSWVLRLGLLSAALGCGLAERPRRARRDPRAVRPPRPAAGP
                     ATCATRAARGRRASPPPPPGGAWEAVRVPRRRQQRAARGAEEPSPPSRALYFSGRGEQ
                     LRLRADLELPRDAFTLQVWLRAEGGQKSPAVITGLYDKCSYTSRDRGWVMGIHTISDQ
                     GNRDPRYFFSLKTDRARKVTTIDAHRSYLPGQWVHLAATYDGRLMKLYMNGAQVATSA
                     EQVGGIFSPLTQKCKVLMLGGSALNHNFRGHIEHFSLWKVARTQREILSDMETRGLHT
                     PLPQLLLQENWDNVKRTWSPMKDGHSPQVEFSNAHGFLLDTNLEPPLCGQTLCDNTEV
                     ISSYNQLPSFRQSKVVRYRVVNIYDDHHENPTVSWQQIDFQHQQLAEAFQHYNISWEL
                     DVLDINSSSLRHRLILANCDISKIGDEKCDPECNHTLTGHDGGDCRQLRYPAFMKKQQ
                     NGACDMDCNYERFNFDGGECCDPDITDVTKTCFDPDSPHRAYLDVNELKNILKLDGST
                     HLNIFFANSSEEELAGVATWPWDKEALMHLGGIVLNPSFYGIPGHTHTMIHEIGHSLG
                     LYHIFRGISEIQSCSDPCMETEPSFETGDLCNDTNPAPKHKFCGDPGPGNDTCGFHGF
                     FDTPYNNFMSYADDDCTDSFTPNQVSRMHCYLDLVYQSWQPSRKPAPVALAPQIVGHT
                     TDSVMLEWFPPIDGHFFERELGSACDLCLEGRILVQYAFNASSPMPCGPSGHWSPREA
                     EGHPDVEQPCKSSVRTWSPNSAVNPHTVPPACPEPQGCYLELEFRYPLVPESLTIWVT
                     FVSSDWDSSGAVNDIKLLTVSGKNISLGPQNVFCDIPLTIRLRDVSEEVYGIQIYTLD
                     EHLEIDAAMLTSAVDSPLCLQCKPLQYKVLRDPPLLDDVGSLLHLNRRFMDMDLKLGN
                     VYQYRIITISGNEESEPSPAAIYTHGSGYCGDGVIQKDQGEECDDMNKVNGDGCSLFC
                     KQEVSFNCIDEPSRCYFHDGDGMCEEFEQKTSIKDCGVYTPQGFLDQWASNASVSHQD
                     QQCPGWVVIGQPAASQVCRTKVIDLSEGISQHAWYPCTINYPYYQLPQTTFWLQTYFS
                     QPMVAAAVIIHLVTDGTYYGDQKQETISVQLLDTKDQSHDLGLHVLSCRNNPLIIPVV
                     HDLSQPFYHSQAVHVSFSSPLVAISGVALRSFDNFDPVTLSSCQRGETYSPAEQSCVH
                     FACEATDCPELTVENASLNCSSSHRYHGAQCTVSCQTGYVLQIQRDDELIKSQVGPSV
                     TVTCTEGKWNKQVACEPVDCGIPDHHHVYAASFSCPEGTTFGRRCSFQCRHPAQLKGN
                     NSFLTCMEDGLWSFPEALCELMCLAPPPVPNADLQTARCRENKHKVGSLCKYKCKPGY
                     HVPGSSRKSKKRAFKTQCTQDGSWQEGTCVPVTCDPPPPKFHGLYQCTNGFQFNSECR
                     IKCEDSDASQGRGSNTIHCRKDGTWSGSFHVCREMQGQCSAPNQLNSHLKLQCPDGYA
                     IGSECATSCLDHNSESIILPVNLTVRDIPHWMNPTRVQRIVCTAGLQWYPHPARIHCV
                     KGCEPFMGDNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQDDCACRDPEA
                     QEHSRKDLRGYSHG"
     exon            1196..2258
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2404
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2698
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            2699..2891
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3013
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3014..3512
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3641
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3642..3733
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3734..3927
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3928..4041
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            4042..4177
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            4178..4391
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            4392..4560
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            4561..4744
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            4745..4955
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            4956..5127
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            5128..5275
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            5276..5385
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            5386..5457
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            5458..5556
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
     exon            5557..12502
                     /gene="Pappa"
                     /gene_synonym="Pappa1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgttttgag ctggaatgag cagagcaggg aggaccgtaa gggggtgtgt acagaattgc
       61 attaaattgt tactttaaga ttgtcttctt taaaaaaaga agaagtagaa gaagaacgag
      121 ggggtggaga agctgaagcg aagggaagga gacaaactgc aaagtagagg aaggctgggt
      181 ggatcggcct ctccgaaccg attgattggt catgatcccc gcagtttgaa cagggactca
      241 ttcaattggg aaggtggagt gctggcgaac agattagcac acgcttgttt actcatcttc
      301 taagggattt ttccccctct ttcctttcat ttcgtgaaga aggagggagg ggagggggcg
      361 gggggggaga agggggctgt ggcttgtgtt ataaaggacg caaaaaataa agaaatgaga
      421 gcatcttggg gggagggagg gaattcagct gatcagtgtg ttagaggaga ttttttttga
      481 agagctagaa atatataaaa taaaatgaaa taaaacaagg aggaaggcat ccagctgtta
      541 gagggggtaa ataaggcaga taaaggagcg gggagagaaa ttaattgcca accaggagga
      601 gttggactgt atttttgaaa ggtgggggga gtggagcaca caccttgagg aggaaagcga
      661 gaaagaaaag gaaaaagcaa gtggaaaggg ggctctcacg agaaggggat cagagactag
      721 gcgggagcta agctggcagc tctgggcggc ggtgcagggg cgcagggggg gcggggggga
      781 ccgtcggaca tgcggctctg gagttgggtg ctgcgcctgg ggctgctgag cgccgcgctg
      841 ggctgcgggc tggccgagcg cccccgccgg gcccggagag accctcgggc cgtgcgcccc
      901 ccgcgccccg ccgccggacc ggccacctgc gccacccggg cggcccgcgg tcgccgcgcc
      961 tcgccgccgc cgcctccggg cggtgcctgg gaagccgtgc gcgtcccccg gcggcggcag
     1021 cagcgggcgg cgaggggcgc cgaggagccg agcccgccga gccgggcgct ctatttcagc
     1081 gggcgagggg agcagctgcg tctccgggcc gacttggagc tgccccgcga cgccttcacg
     1141 ctgcaagtgt ggctgagagc cgaaggtggc cagaagtctc cagccgtgat cacagggctg
     1201 tatgacaaat gttcttatac ctcgcgtgat cgaggatggg tcatgggcat tcacaccatc
     1261 agtgatcaag gcaacagaga tccacgctac tttttctcct tgaagacaga ccgggccagg
     1321 aaagtaacca ccattgatgc ccatcgcagc tacctcccgg gtcagtgggt gcacctagct
     1381 gctacctacg atgggcgact gatgaagctc tatatgaatg gtgcccaggt ggcgacctca
     1441 gcggagcaag taggtggcat attcagccca ctgacccaga agtgtaaagt gcttatgttg
     1501 gggggcagtg ctctgaatca caacttccgg ggccacattg aacacttcag tctatggaaa
     1561 gtagccagaa cccagcgaga gatactatcc gacatggaaa cgcgtggcct ccacacccct
     1621 ctacctcagc tcctcctcca ggagaactgg gacaatgtga agcgcacttg gtcccctatg
     1681 aaggatggcc acagccccca ggtggaattc agcaatgcac atggcttcct gttggacact
     1741 aatttggagc cccctctttg cgggcagaca ctgtgtgaca acacagaggt catctccagt
     1801 tacaatcagc tcccaagttt tcggcagtcc aaggtggtgc gctatcgtgt ggtcaacatc
     1861 tatgatgatc accatgaaaa ccctacagtg agctggcagc agattgactt tcagcaccaa
     1921 cagctggctg aggccttcca gcactacaac atctcctggg agttggacgt actagatata
     1981 aacagttcct ctctgcgtca ccgcctcatc ctggccaact gtgacatcag caagattggg
     2041 gatgaaaaat gcgaccctga atgtaaccat acactgactg gccatgacgg tggggattgc
     2101 cggcagctgc gctaccctgc attcatgaag aagcagcaga atggcgcatg tgacatggac
     2161 tgcaactacg aaaggtttaa ttttgatggt ggagagtgct gtgacccaga catcactgat
     2221 gtcactaaga catgctttga tcctgactct ccacacagag cctacttgga tgttaatgag
     2281 ctaaagaaca ttcttaaact ggatggatca acacatctca atattttctt tgcaaactct
     2341 tcagaggagg agttggcagg agtggcaact tggccatggg acaaggaggc cctaatgcac
     2401 ttgggcggta ttgtcttgaa cccgtctttc tatggcattc ctggacacac ccacaccatg
     2461 atccatgaga ttgggcatag cctgggcctc tatcacatct tccgtggtat ctcagaaatc
     2521 cagtcctgca gtgatccttg catggagaca gagccctcat ttgaaactgg agacctctgc
     2581 aatgacacca acccagcccc taaacacaag ttttgtggag accctggacc agggaatgac
     2641 acttgtggct ttcatggctt cttcgacact ccttataaca acttcatgag ctatgcagat
     2701 gacgactgta cagactcttt cacgcccaat caagtctcca gaatgcactg ttacctggac
     2761 ctcgtatacc agagctggca gccctccaga aagccagcac ctgtagctct tgcaccccag
     2821 attgtgggcc acacaaccga ctctgtgatg ctagagtggt tcccacccat cgatggccac
     2881 ttctttgaaa gagaattggg atcagcatgt gacctttgcc tagaagggag aatcctggtg
     2941 caatatgctt tcaatgcttc ctcccccatg ccctgtggac catcgggaca ctggagtcct
     3001 cgggaagcag aaggtcaccc agatgttgaa cagccctgta aatccagtgt ccgtacctgg
     3061 agtccaaatt cagctgtcaa cccacacaca gtccctccag cctgccctga gccacaaggc
     3121 tgctacctcg agctggaatt ccgctacccc ttggtccctg agtctctgac catctgggta
     3181 acctttgtct ccagtgactg ggactccagt ggagctgtca atgacatcaa actcttgact
     3241 gttagtggaa agaacatctc tttgggtcct caaaatgtct tctgtgatat cccacttacc
     3301 atcagactcc gagatgtgag tgaggaggta tatggcatcc aaatctatac tctggatgaa
     3361 cacctggaaa ttgatgcagc catgttgacc tccgctgtag acagcccact ctgtctacag
     3421 tgtaaacccc tgcaatacaa agtgctcaga gacccacctc tgctggatga cgtaggctca
     3481 ttactccacc tcaacagaag attcatggat atggatctga aacttggcaa tgtgtaccag
     3541 taccggatta tcaccatatc aggaaacgaa gagagtgagc cctcacctgc tgccatatac
     3601 acccatggaa gtgggtactg tggtgatggc gttattcaaa aagaccaagg ggaagaatgt
     3661 gatgatatga ataaggtcaa cggggatggc tgctcccttt tctgcaagca agaagtttcc
     3721 ttcaactgca ttgatgaacc cagccggtgc tatttccatg atggggatgg gatgtgtgaa
     3781 gagtttgagc aaaaaactag cattaaagac tgtggtgtct acacgcccca gggtttcctg
     3841 gatcagtggg cgtccaatgc ttcagtatcc catcaagacc agcagtgccc aggttgggtt
     3901 gtcattgggc agccggcagc ctctcaggtg tgtcgaacca aggtgataga tctcagtgaa
     3961 ggcatttccc agcatgcttg gtatccttgc accatcaatt acccatacta tcagctgcct
     4021 cagaccacat tctggctcca gacatatttc tctcagccaa tggtggctgc agctgttatt
     4081 attcacctgg tgactgatgg gacatactat ggggaccaaa agcaagagac catcagtgtt
     4141 cagttgcttg ataccaaaga tcaaagccac gatttaggcc tccatgtctt gagctgcaga
     4201 aacaatcccc tgattatccc tgtggtccat gacctcagcc agcccttcta ccacagccag
     4261 gcggtacatg tgagcttcag ttcgcccctg gtcgccatct cgggggtggc cctccgatcc
     4321 ttcgacaact ttgaccccgt caccctgagc agctgccaga gaggagagac ctacagccct
     4381 gctgagcaga gctgtgtgca ttttgcctgt gaagccacgg actgcccaga actgactgtg
     4441 gagaatgctt ctctcaactg ttccagcagc catcgttatc atggtgctca gtgcaccgta
     4501 agctgccaga caggttatgt gctgcagata cagcgagatg atgagctaat caagagccag
     4561 gtagggccaa gcgtcacagt gacatgtaca gagggcaaat ggaacaagca ggtggcatgt
     4621 gagcctgtag actgcggtat cccggatcac catcacgtct acgccgcctc cttctcctgt
     4681 ccagagggta ccacctttgg tagaagatgc tcctttcagt gtcgccaccc tgcccagctg
     4741 aaaggcaaca acagctttct gacctgtatg gaagatggac tgtggtcctt cccagaggca
     4801 ctgtgtgagc ttatgtgcct cgccccaccc ccagttccca atgcggacct acagacagcc
     4861 cggtgtcgcg agaacaagca caaggtgggc tccttatgca agtacaagtg caaacctgga
     4921 taccatgtgc ctggctcatc tcggaagtcc aagaaacggg ccttcaagac tcaatgtact
     4981 caagacggca gctggcaaga gggaacatgt gtgccggtga cttgtgaccc acctccaccc
     5041 aaattccatg ggctctatca gtgcactaat ggcttccagt tcaatagtga gtgtaggatc
     5101 aagtgtgaag acagtgatgc ctcccagggc cgtgggagca acaccattca ctgccggaaa
     5161 gacggcactt ggagtggttc cttccatgtc tgccgagaga tgcaaggcca gtgctcagcc
     5221 ccaaaccaac tcaacagtca cctcaaattg cagtgtcctg acggctatgc catagggtca
     5281 gagtgtgcca cctcgtgcct ggaccacaac agcgagtcca ttatcctgcc tgttaacttg
     5341 acggtgcgtg acattcccca ttggatgaac cccacacgag tacagaggat tgtctgcact
     5401 gctggtctcc agtggtatcc ccaccctgct cggatccact gtgtcaaagg ctgtgagcca
     5461 ttcatgggag acaattactg tgatgccatc aacaatcgag ccttctgcaa ctatgatggt
     5521 ggggactgct gcacctccac agtaaagacc aaaaaggtca ctccctttcc tatgtcctgt
     5581 gacctacaag atgactgcgc ctgtcgggac cctgaggccc aagaacacag ccggaaagac
     5641 cttcgtggat atagccatgg ctaaggaaag acaggtgtca aagatttccc aacgccagga
     5701 catgcatccc tctggtattg atttcacagt ctgctgctca atgaaaaggc ctctccacac
     5761 cagggatcct tagcacccaa ccggtctgcc tttaatttca cccaggaagg accaactgtg
     5821 gggcaatgaa ccgagtctca ccatgttgaa tgatggagtg gaatactatc ctgtaatcta
     5881 agatggattg cacacacagc atgtagtccc agagcctctg aaagttcttt ccatttttca
     5941 catgaccaca gccccaaaca acgaaattgt gtgtgtatca aggagtatgc acatctgtgg
     6001 ttagtacaca ggcatgcata cacacccata ctaatgtctg tgtgagggta cttttggaaa
     6061 ctgagtagga aaaaaaactg gaaaaaatga acaaaatgaa aaaaaaatca atctttcatt
     6121 tcccaagctc ccagtgagca cagcccttgg gagaagtggg ttatagaaac taagcccaag
     6181 tactgaaaag ccaatcttag aatgtgaggc tcagaatatg tagtatgtta catcattgtg
     6241 tagtgctgtg ggattggagg taaaatggtt gtagtcacca accttctagg aaactatgcc
     6301 tccccaaatg gtaaatactc caaggtgtgc aaagctcacc aatatttttg ctgccatggg
     6361 ggttttgtcc caacaccagg gtgcaaggct gagctggaaa tggtgtagga ctgcagaatt
     6421 aaggtattct atcagctccc tgcttgagac ttgaggtctt catacccctt ccagatccca
     6481 cccatttttc agatcttacc ttgggaccac atttgctgct actcttcctg ctgctctgtc
     6541 caaatacatt gagagcccta agatggtagg actaggttag aacagactcc atataaccaa
     6601 gtagactgcc tcaaaagtga atcatatttt ctcacagttc ctcacctctt ggcccatcca
     6661 caagaaaaaa aagaaaaaca ttcatgggct taagtctttc atgagtagac ttttcatttt
     6721 ggaaaagagt tgcgggatgc cagatgctgg agccctttct gaaagtccag tatgtctaag
     6781 ccagtgttag attttctaat cgagtggaac agtgttaaac ttgggaatgc tggagctacc
     6841 cagagactgt tggaatagca agaacttgag ttattgtcct tatcccagtc ttcctagctt
     6901 tctaggctag aataagcttt ggtcttcaga ctattccatt actcccagca gatccagttt
     6961 tgaaacatct tggatattgg caatgtagac tatatactaa aggaatatgg aaaggaagcc
     7021 cttcagatcg agactgagca cccaagatag ccatcatatt ttccacataa tttttcagat
     7081 gttaggacat atttctacgt ccattgctgg gcaccgctcc ttccactccc atatttagtc
     7141 tttaaatgtt aagaggaaat gagttaattg cctatggaag ccagcaatgt atgcagggcc
     7201 ttttttttct gcaccttttc agttacctat atatacactc tggattaata tgggaagaga
     7261 ttaataaaga ataatatgga tagaatagct aactttgaaa taatttatga aaggtggaga
     7321 aggaagcaat gctatgtgag gatctaaatg aattatataa caaagatgat agcatatcaa
     7381 ttgatctcaa gtctagtgga gatagccaat aaaagcaaag tctgggacag aatgcaaaat
     7441 gccagcccag cattaattgt taatctatca tatccctttt tctactagga acaacagtaa
     7501 ctttgccaac tgtgttttca tattgtgatc aataactttt tcctgctgat caagaacact
     7561 tgaaaaggta cctactgacc atctgtggtc cataatatca agaaatgtac caaggtgttt
     7621 ttgcactggt acccataaat atggatacag tgcttatatt atgcatcttg tcctgtcctt
     7681 tagcctctga ggttgaagtc tagagtggaa aagtggccac caatgccggg gtcaagcact
     7741 tcagctcctc caaaagcaaa tctcatttat acatagggca tttccctttg ggattttcag
     7801 accaatctat agcaaagtat aagaacaatc ttttggagta ctggcatgat tatgatcatc
     7861 tctccaagat cctcaaactt ttgtcaaccc ttcaggtccc tctcttgctc agatgtgctg
     7921 tgtcagctta gaagggacaa gcaaaagaat ggtgacagag ctatccctcc tgataagtaa
     7981 cccagaaatt tccaaagacc caaagataac attatcagta ggagggttat agaagccaag
     8041 aaaaccacaa gaaacttctt ccaatcaatc tttatccaac tctatcttgg gtctccctgt
     8101 catctgttct tatactctac agctgggcaa agattcctgg aggctggctc ctctattaag
     8161 attcatagac tgaattgggg ggtgggtgct ttccaggaag agtcccatcc agtttcttcc
     8221 ttattaccat tcaagcctgt taagaacaaa ttcatatttg ctgagcctaa tagataaaag
     8281 aggctaccca attggtagct cgcctcatgc ccacaaggga tgtgtaattc ataaggtctt
     8341 ctcagacctt agtgcaggac agtcagctgt atttttgcaa tgcactgatt cccagtttca
     8401 ttttcttcct gagaataaat tccttgtcac atctcctcag tcatctgttt tggacaatgg
     8461 gataatcagg aggaggcggg tctgaagcac tattgtacat ccacagctta gggtgcccta
     8521 aagcgcttga gagttctgca gctgaaacag tataagtcgg ttagcagcaa ggaagcagag
     8581 atgagatgga cagttctctg agctccatct gggaaactgt tccaaaccgc aacacactgt
     8641 ctgcccagag gacttgcatt tccccctaga ttttgtgcca acagcctcat gagggaagtc
     8701 agtgtgggaa gtcagtcagt ccttgggaag cagtgtgggt aggtgaagtg gggtgtagag
     8761 taaaggagag attaaaggtc ttaaatagca ggcctttgga gaaggaggga accagctgct
     8821 gctcttggcc tcagcacact gggccattgc cgagaccatt ctctgtctca ttcccacgtt
     8881 ggccaatgct taaatgctat ttgttgaaaa taattctttg ggacagattt cagctacctc
     8941 ccttccaggt ttgatttaac ttggttgtaa ctgtcaattt gttaacggtt ttacctgtgt
     9001 ggatttttta aaaaagaaac aaatagaaaa agacgtgaaa gagaagaaaa gaacaggaag
     9061 gaaagcagtc atattagctg tttctgagtg tgtgtgtgtg tgtgtgtgtg tgtgtgagtc
     9121 tgtgtgtatc tgtgtgtctc tatgtgtgag tctctgtgtg tgtttgtgtg attgtttttc
     9181 ctgaaactac ttcaagtgag aaaataaata gaaggaattg aaggtgacaa aactgtaaaa
     9241 attgaaatta atagaagaca aagtgattac aagggactaa aatgcattat taaagtctaa
     9301 gaatatgaac cctgttttta tgcttcctgg aggagactat tctgtagact gagtagatag
     9361 agatgacata gagatttctc tagaaaatgt agagtgggat tctatggctc tgactaaccc
     9421 tttctgaaca ttgtggtagg tccaggaagt aaggcatttt ctcccttgag ttgttgtcag
     9481 ctaagcattt ggttcatttc tttggtgact gtctatggta gaggggacag gggaaggttg
     9541 atattgatag ttaccaaata ttaaggatag aaactgtgtc tttgtccttt tttactctat
     9601 acgatatttg tatcggccag tatagccctg agctccctaa agactgcact cagaaccaca
     9661 ctgcatagct ctgttcttca gagctgctcc atgaccaggc aggtgatccc actgaatact
     9721 gagaaaggat caaaccaagt aaacaggtaa tacagagaac aaattgcatg aaatgcctct
     9781 ttttgattta ttcaagtttt ccaaattcct agctatcatt tcctctgtaa aagcaaggta
     9841 ataatgacca agatagaagt tgatgtgtct ggcccaaagc cccagaatgc ttgcctgcag
     9901 ccatcaattc actcggtgtt cttggataag tcatctaatt gcctccacat ttgcctcatt
     9961 ttctctagct gaaaattggg gatactgcta cttacctacc tcacagtggg aggatcatga
    10021 tgccctgagg attttaaacg gactgtccaa tctataggag acacagcctg atgcaaaatc
    10081 catagtttcc tccccccttt ttttttgttt tttttttttt caaaaacgta caggctcact
    10141 gcccttttca gagacaggga gtgcttgaat gacatttcaa ggcacaaagc tggagggtct
    10201 ggattaccat ccaccttaag tgagtgtact ggggaatagt ttactctttg gataagattt
    10261 ccctccatta ttctggcatt taccaatcca atgatttgat caaaatccag tggcttccta
    10321 cagtataaaa gtcagtctac tgtcatctag ctaggaatgc taagattggg ggtggaggca
    10381 tttccaagtt acagactgag aaggtctgcc atttcttctt caacagaatc ctcacttctc
    10441 agacttagtt tccaaatcac aggcttcatt aagagaagag ataacatcct gagtgcaagt
    10501 tgggcttctt caacttcaca ccagcagcta gtgaggagaa ctttgaacaa ttattgagtt
    10561 gctttcttgg gtctctataa tcaataacct ttctgcagat atctatctat ataaagatat
    10621 tatatataaa taaaaattta catatatatg cacatgtata tatagctgta catatatgtg
    10681 tgtatatata tacttaaatg taatatttac aaaactgtga tctcgtctag agaaaatgta
    10741 ttcatattac aaactgctct tccatattta tgtatcatat ttaccttttt attactgtcg
    10801 taattattga gggtatttct aattattatg atgttaggat ctgtttagca tcttctggaa
    10861 gcaaccaaaa aatatgactt ttcattgaag tgcttagaat ctgttctcat gagaattcca
    10921 ctggtctact gtaaaacaaa gaaagaaata aaaagaaaca aaaaatgaaa gaaaggaaga
    10981 aagagagaga aaagaaagat taaagcaaag taaacagcaa aaactaaata caaccccaac
    11041 cttcttcctg cacctcgcgc gcagaaatgt gctgctcttg ttttgggatt tatttttcct
    11101 gtttatgtgt ttttatggat ctaagttaag tctttcagca atatataaaa atgtaaatag
    11161 ttaactttat ttattaagaa tgtcatcttt tttaatttat atttacacga tggttcatct
    11221 aatttatttt ttctatacgg ttttaaatac tcaaacatat ttttctgttc atggtaattt
    11281 ttaagctctt ctcatggatt ggtgtttcca tactgttttc tctggtctca attacaggtt
    11341 agatctgaca cacacacaca cacacacaca cacacacaca caactaatat caaagacaaa
    11401 tattttgcca ctgttgatta ctatacctta aagttctata ttatgaaaat atataatagc
    11461 ttgtctgatt cagcatgttg ttgtgtggct tttgactgct atagagatac atagaatggt
    11521 catgtgtgtg cacacaaaca cgctcaaaca cacaggaaca cacacccatg caataacatg
    11581 tgagcttata atttaaaaaa tcattaaaaa agcatgaaat tgaattcaat atatatatca
    11641 agtcctctat ataattcctg aaaagtatct atattttgta catgcaaaac ctggcaaaca
    11701 ttattataca ttgtaatata tttccattct atggcactaa agcatatact ctgtgtcctt
    11761 atatactact atttggactc tgtcttcaac aatatactct gagaataatt tttattttca
    11821 taacaaacaa taaacaaaca acaaaaaagg aacacaccct gtgttcttgg tgtaaccctg
    11881 tccttgtaat cctgagctca ctacccaggt tgatactgca caaaccctgc agaaatctgg
    11941 tccttcaaca ttacatcacg gatcagggac tgagcggctg gttatgatgc ctctctccct
    12001 aaagagctat tgacaggtca ttgttgcagg gggagagagg tatcattttc tccagtgtca
    12061 taagaattgg caaggggccc atgcttcaat aaataaccct ctctctaagc tcatccaagc
    12121 aaaccttatt aaataaatgt taggaccaac aatatcaaca acaacaaaaa gtgagaaaac
    12181 taagaagaag ggcatgtgca aggcagggga ggggagagag agggaggagt ggataggatc
    12241 acaacacaat gtatatgtat aaagttttta aaatgatgtt atgtgaaaaa taataaatta
    12301 aaaagaacga aaaattaacc acccagtcca tcttgtgaac ctgcactgag actggatatt
    12361 gctgctttct ctctgtttag cacattttat tgttcatcag tacccagcta gtaagtagct
    12421 atgcagtctt tggaaagtta ttaaattgtt ttaaggtttt gttttactat ctgtgaaatg
    12481 aaaataaact atcaacttta ta
//
