LOCUS       NM_017198               2535 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus p21 (RAC1) activated kinase 1 (Pak1), mRNA.
ACCESSION   NM_017198
VERSION     NM_017198.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Xu,L., Yang,L., Wu,Y., Wan,X., Tang,X., Xu,Y., Chen,Q., Liu,Y. and
            Liu,S.
  TITLE     Rac1/PAK1 signaling contributes to bone cancer pain by Regulation
            dendritic spine remodeling in rats
  JOURNAL   Mol Pain 19, 17448069231161031 (2023)
   PUBMED   36938611
  REMARK    GeneRIF: Rac1/PAK1 signaling contributes to bone cancer pain by
            Regulation dendritic spine remodeling in rats.
            Erratum:[Mol Pain. 2023 Jan-Dec;19:17448069231182501. doi:
            10.1177/17448069231182501.. PMID: 37608691]
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Ma,W., Wang,X., Wang,C., Gong,M. and Ren,P.
  TITLE     Up-regulation of P21-activated kinase 1 in osteoarthritis
            chondrocytes is responsible for osteoarthritic cartilage
            destruction
  JOURNAL   Biosci Rep 40 (1) (2020)
   PUBMED   31868209
  REMARK    GeneRIF: Up-regulation of P21-activated kinase 1 in osteoarthritis
            chondrocytes is responsible for osteoarthritic cartilage
            destruction.
REFERENCE   3  (bases 1 to 2535)
  AUTHORS   Zhao,H. and Tao,S.
  TITLE     MiRNA-221 protects islet beta cell function in gestational diabetes
            mellitus by targeting PAK1
  JOURNAL   Biochem Biophys Res Commun 520 (1), 218-224 (2019)
   PUBMED   31587871
  REMARK    GeneRIF: MiRNA-221 regulates proliferation, apoptosis and insulin
            secretion in islet beta cells through targeting PAK1, thus
            protecting gestational diabetes mellitus -induced islet dysfunction
            in a rat model
REFERENCE   4  (bases 1 to 2535)
  AUTHORS   Tunduguru,R., Zhang,J., Aslamy,A., Salunkhe,V.A., Brozinick,J.T.,
            Elmendorf,J.S. and Thurmond,D.C.
  TITLE     The actin-related p41ARC subunit contributes to p21-activated
            kinase-1 (PAK1)-mediated glucose uptake into skeletal muscle cells
  JOURNAL   J Biol Chem 292 (46), 19034-19043 (2017)
   PUBMED   28972183
  REMARK    GeneRIF: These results expand the model of insulin-stimulated
            glucose uptake in skeletal muscle cells by implicating p41ARC as a
            new component of the insulin-signaling cascade and connecting PAK1
            signaling to N-WASP-cortactin-mediated actin polymerization and
            GLUT4 vesicle translocation.
REFERENCE   5  (bases 1 to 2535)
  AUTHORS   Chang,J.K., Ni,Y., Han,L., Sinnett-Smith,J., Jacamo,R., Rey,O.,
            Young,S.H. and Rozengurt,E.
  TITLE     Protein kinase D1 (PKD1) phosphorylation on Ser203 by type I
            p21-activated kinase (PAK) regulates PKD1 localization
  JOURNAL   J Biol Chem 292 (23), 9523-9539 (2017)
   PUBMED   28408623
  REMARK    GeneRIF: PAK-mediated phosphorylation of PKD1 at Ser203 triggers
            its membrane dissociation and subsequent entry into the nucleus,
            thereby regulating the phosphorylation of PKD1 nuclear targets,
            including class IIa histone deacetylases.
REFERENCE   6  (bases 1 to 2535)
  AUTHORS   Hirai,S., Izawa,M., Osada,S., Spyrou,G. and Ohno,S.
  TITLE     Activation of the JNK pathway by distantly related protein kinases,
            MEKK and MUK
  JOURNAL   Oncogene 12 (3), 641-650 (1996)
   PUBMED   8637721
REFERENCE   7  (bases 1 to 2535)
  AUTHORS   Manser,E., Chong,C., Zhao,Z.S., Leung,T., Michael,G., Hall,C. and
            Lim,L.
  TITLE     Molecular cloning of a new member of the p21-Cdc42/Rac-activated
            kinase (PAK) family
  JOURNAL   J Biol Chem 270 (42), 25070-25078 (1995)
   PUBMED   7559638
REFERENCE   8  (bases 1 to 2535)
  AUTHORS   Martin,G.A., Bollag,G., McCormick,F. and Abo,A.
  TITLE     A novel serine kinase activated by rac1/CDC42Hs-dependent
            autophosphorylation is related to PAK65 and STE20
  JOURNAL   EMBO J 14 (9), 1970-1978 (1995)
   PUBMED   7744004
  REMARK    Erratum:[EMBO J. 1995 Sep 1;14(17):4385. doi:
            10.1002/j.1460-2075.1995.tb00113.x.. PMID: 7556080]
REFERENCE   9  (bases 1 to 2535)
  AUTHORS   Manser,E., Leung,T., Salihuddin,H., Zhao,Z.S. and Lim,L.
  TITLE     A brain serine/threonine protein kinase activated by Cdc42 and Rac1
  JOURNAL   Nature 367 (6458), 40-46 (1994)
   PUBMED   8107774
REFERENCE   10 (bases 1 to 2535)
  AUTHORS   Williams,A.F., Barclay,A.N., Letarte-Muirhead,M. and Morris,R.J.
  TITLE     Rat thy-1 antigens from thymus and brain: their tissue
            distribution, purification, and chemical composition
  JOURNAL   Cold Spring Harb Symp Quant Biol 41 Pt 1, 51-61 (1977)
   PUBMED   70317
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Nov 26, 2020 this sequence version replaced NM_017198.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U49953.1, SRR26643293.4397.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-364               JAXUCZ010000001.1  161522450-161522813
            365-578             JAXUCZ010000001.1  161585186-161585399
            579-679             JAXUCZ010000001.1  161593132-161593232
            680-827             JAXUCZ010000001.1  161593737-161593884
            828-865             JAXUCZ010000001.1  161598204-161598241
            866-982             JAXUCZ010000001.1  161611958-161612074
            983-1157            JAXUCZ010000001.1  161614765-161614939
            1158-1221           JAXUCZ010000001.1  161616758-161616821
            1222-1270           JAXUCZ010000001.1  161620217-161620265
            1271-1383           JAXUCZ010000001.1  161624255-161624367
            1384-1501           JAXUCZ010000001.1  161626011-161626128
            1502-1601           JAXUCZ010000001.1  161629277-161629376
            1602-1798           JAXUCZ010000001.1  161630287-161630483
            1799-1936           JAXUCZ010000001.1  161633214-161633351
            1937-2535           JAXUCZ010000001.1  161636354-161636952
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..2535
                     /gene="Pak1"
                     /note="p21 (RAC1) activated kinase 1"
                     /db_xref="GeneID:29431"
                     /db_xref="RGD:3250"
     exon            1..364
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            365..578
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     CDS             389..2023
                     /gene="Pak1"
                     /EC_number="2.7.11.1"
                     /note="PAK-1; p68-PAK; alpha-PAK; protein kinase MUK2;
                     p21-activated kinase 1; p21 (CDKN1A)-activated kinase 1;
                     p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related);
                     p21 protein (Cdc42/Rac)-activated kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 1"
                     /protein_id="NP_058894.1"
                     /db_xref="GeneID:29431"
                     /db_xref="RGD:3250"
                     /translation="MSNNGLDVQDKPPAPPMRNTSTMIGAGSKDPGTLNHGSKPLPPN
                     PEEKKKKDRFYRSILAGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQ
                     WARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNTLN
                     VKTVSETPAVPPVSEDEDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVAT
                     SPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIG
                     QGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYL
                     VGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSD
                     NILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLG
                     IMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKR
                     GSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH"
     exon            579..679
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            680..827
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..865
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            866..982
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1157
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1221
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1270
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1383
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1501
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1601
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1798
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1936
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2535
                     /gene="Pak1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccgggcgga aggtaccgcc gcacggtggg cgctcctcac cgcttcccca cacctcccag
       61 tcccactgca cctctaggct gcgcggagtc ctctcggcta ttaccggctc tttgcagccg
      121 cctgtgcccc ctcagagccc gcgctctcca cagtctcctc agagaaggac cccctcagcg
      181 agcgcacccc gccccttcgc agtccgggcg cgcctcccgc tccccgcggc ccgcagcgtc
      241 ccgccctcca gagcccggct cgagcggcga gaggagaacg cggggctgcc gcccgcgcac
      301 actcccgcgc tctctctggg tttggctgcc gccaggagga ggaggagcgg agcgagcgca
      361 gaagtagctg ctgctgctgg tggtgacaat gtcaaataac ggcttagacg tccaggacaa
      421 acccccagcc cctccgatga gaaacaccag cactatgatt ggagctggca gcaaagaccc
      481 tggaacccta aaccacggtt ccaaacctct gcctccaaac ccagaggaga agaaaaagaa
      541 ggaccggttc tatcgatcca tcttagctgg agataaaaca aataaaaaga aggagaagga
      601 gcggccagag atttctcttc cttcagattt tgagcataca attcatgttg gttttgatgc
      661 tgtcacaggg gagtttacgg ggatgccaga acagtgggcc cgcttgcttc aaacatcaaa
      721 tatcaccaag tcagagcaga agaaaaaccc acaggctgtt ctggatgtgt tggaatttta
      781 taactccaag aagacctcca atagtcagaa gtacatgagt tttacagata agtcagctga
      841 agattataat tcttctaata ctttgaatgt gaagactgtg tctgagaccc cagcagtgcc
      901 accagtgtca gaagatgaag atgatgatga cgatgctacc ccacctccag tgattgctcc
      961 acgcccagaa cacacaaaat ctgtatatac acggtctgtg attgaaccac ttcctgttac
     1021 tccaactcgg gatgtggcta catctcctat ttctcctact gagaataaca ccactccgcc
     1081 agatgctttg acccggaata ctgaaaagca gaagaagaag cctaaaatgt ctgatgagga
     1141 gatcttagag aaattacgga gcatagtgag tgtgggcgat cctaagaaga agtacacacg
     1201 cttcgagaag attggacaag gtgcttcagg cacagtgtac actgcaatgg atgtagccac
     1261 agggcaggag gtggccatta aacagatgaa ccttcagcag cagccgaaga aagaactcat
     1321 tattaatgag atcctggtca tgagggaaaa caaaaaccca aacattgtga actatctgga
     1381 cagttacctt gtgggagatg agctatgggt tgtcatggaa tacttggccg gaggctcctt
     1441 aacagatgtg gtgacagaaa cctgtatgga tgaaggccag atagcagctg tgtgccgaga
     1501 gtgtctacaa gctttggagt tcctgcattc aaaccaagtc attcacagag acatcaagag
     1561 tgacaatatt ctgctgggaa tggatggctc tgtcaaatta actgactttg gattctgtgc
     1621 acagataact ccagagcaga gcaaaaggag caccatggtg ggaactccat attggatggc
     1681 acctgaagtt gtgacacgca aggcctatgg acccaaggtt gacatctggt ccctgggtat
     1741 tatggcaatt gaaatgattg agggggagcc cccatacctc aatgaaaacc ctttgagagc
     1801 cttgtacctc attgctacca atgggacgcc agagcttcag aacccagaga agttgtcagc
     1861 tattttccgg gactttttga accgctgtct tgagatggat gtggagaaga gaggttcagc
     1921 taaagagcta ctgcagcatc aattcctgaa gattgccaag cctctctcca gcctgactcc
     1981 actgattgct gcagcaaaag aggcaaccaa gaacaatcac tgaaaccacg ctcaccccag
     2041 cctcatgtgc caagccttct atgaaataaa cactcgtttc gggaactccg acccctcatg
     2101 tcctcttctc ctttccttgc ttctcccatt tcctgatcta gtgctctcaa gactttgatc
     2161 cttggaaacc gtctagcact gaagagaacc gcaaccggat gactaatcca gcagaggcca
     2221 tttctaaata ggaattccct ttagcttgtg ggcatggagg ggactgatga gcaagggttt
     2281 acctgaataa acctgtttct acgaaacaga aatctcaacc atcccattcc ttacccctca
     2341 caatcagttc ttaactctat aaacttatgg tttgatagca ttatcaattt gctatcagtt
     2401 gaaattgctt tcgtttttta tttctgtgac caaattgccc aaacacttca ttgtatttga
     2461 aaaccagaac agctttgaaa tgccacgggg cctgataatc tgccagggac atgaagaggc
     2521 tctgtttcct gaacc
//
