LOCUS       NM_022253               1353 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus phosphoprotein membrane anchor with
            glycosphingolipid microdomains 1 (Pag1), mRNA.
ACCESSION   NM_022253
VERSION     NM_022253.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Tanaka,H., Akagi,K., Oneyama,C., Tanaka,M., Sasaki,Y., Kanou,T.,
            Lee,Y.H., Yokogawa,D., Dobenecker,M.W., Nakagawa,A., Okada,M. and
            Ikegami,T.
  TITLE     Identification of a new interaction mode between the Src homology 2
            domain of C-terminal Src kinase (Csk) and Csk-binding
            protein/phosphoprotein associated with glycosphingolipid
            microdomains
  JOURNAL   J Biol Chem 288 (21), 15240-15254 (2013)
   PUBMED   23548896
  REMARK    GeneRIF: an allosteric connection between Cbp binding and Csk
            activation that arises from residues in the betaB/betaC loop of the
            SH2 domain.
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Dey,N., Howell,B.W., De,P.K. and Durden,D.L.
  TITLE     CSK negatively regulates nerve growth factor induced neural
            differentiation and augments AKT kinase activity
  JOURNAL   Exp Cell Res 307 (1), 1-14 (2005)
   PUBMED   15890337
  REMARK    GeneRIF: Csk negatively regulates growth factor induced neural
            differentiation and augments Akt kinase activity.
REFERENCE   3  (bases 1 to 1353)
  AUTHORS   Matsuoka,H., Nada,S. and Okada,M.
  TITLE     Mechanism of Csk-mediated down-regulation of Src family tyrosine
            kinases in epidermal growth factor signaling
  JOURNAL   J Biol Chem 279 (7), 5975-5983 (2004)
   PUBMED   14613929
REFERENCE   4  (bases 1 to 1353)
  AUTHORS   Davidson,D., Bakinowski,M., Thomas,M.L., Horejsi,V. and
            Veillette,A.
  TITLE     Phosphorylation-dependent regulation of T-cell activation by
            PAG/Cbp, a lipid raft-associated transmembrane adaptor
  JOURNAL   Mol Cell Biol 23 (6), 2017-2028 (2003)
   PUBMED   12612075
REFERENCE   5  (bases 1 to 1353)
  AUTHORS   Ohtake,H., Ichikawa,N., Okada,M. and Yamashita,T.
  TITLE     Cutting Edge: Transmembrane phosphoprotein Csk-binding
            protein/phosphoprotein associated with glycosphingolipid-enriched
            microdomains as a negative feedback regulator of mast cell
            signaling through the FcepsilonRI
  JOURNAL   J Immunol 168 (5), 2087-2090 (2002)
   PUBMED   11859092
  REMARK    GeneRIF: negative feedback regulator of mast cell signaling through
            the FcepsilonRI
REFERENCE   6  (bases 1 to 1353)
  AUTHORS   Takeuchi,S., Takayama,Y., Ogawa,A., Tamura,K. and Okada,M.
  TITLE     Transmembrane phosphoprotein Cbp positively regulates the activity
            of the carboxyl-terminal Src kinase, Csk
  JOURNAL   J Biol Chem 275 (38), 29183-29186 (2000)
   PUBMED   10918051
REFERENCE   7  (bases 1 to 1353)
  AUTHORS   Brdicka,T., Pavlistova,D., Leo,A., Bruyns,E., Korinek,V.,
            Angelisova,P., Scherer,J., Shevchenko,A., Hilgert,I., Cerny,J.,
            Drbal,K., Kuramitsu,Y., Kornacker,B., Horejsi,V. and Schraven,B.
  TITLE     Phosphoprotein associated with glycosphingolipid-enriched
            microdomains (PAG), a novel ubiquitously expressed transmembrane
            adaptor protein, binds the protein tyrosine kinase csk and is
            involved in regulation of T cell activation
  JOURNAL   J Exp Med 191 (9), 1591-1604 (2000)
   PUBMED   10790433
REFERENCE   8  (bases 1 to 1353)
  AUTHORS   Kawabuchi,M., Satomi,Y., Takao,T., Shimonishi,Y., Nada,S.,
            Nagai,K., Tarakhovsky,A. and Okada,M.
  TITLE     Transmembrane phosphoprotein Cbp regulates the activities of
            Src-family tyrosine kinases
  JOURNAL   Nature 404 (6781), 999-1003 (2000)
   PUBMED   10801129
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB038038.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB038038.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q23"
     gene            1..1353
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /note="phosphoprotein membrane anchor with
                     glycosphingolipid microdomains 1"
                     /db_xref="GeneID:64019"
                     /db_xref="RGD:620394"
     exon            1..205
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1349
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /note="csk-binding protein; transmembrane phosphoprotein
                     Cbp; Csk binding protein; phosphoprotein associated with
                     glycosphingolipid microdomains 1"
                     /codon_start=1
                     /product="phosphoprotein associated with
                     glycosphingolipid-enriched microdomains 1"
                     /protein_id="NP_071589.1"
                     /db_xref="GeneID:64019"
                     /db_xref="RGD:620394"
                     /translation="MGPAGSALSSGQMQMQMVLWGSLAAVAMFFLITFLILLCSSCDR
                     DKKPRQHSGDHESLMNVPSDKEMFSHSATSLTTDALASSEQNGVLTNGDILSEDSTMT
                     CMQHYEEVQTSASDLLDSQDSTGKAKCHQSRELPRIPPENAVDAMLTARAADGDSGPG
                     VEGPYEVLKDSSSQENMVEDCLYETVKEIKEVADKSQGGKSKSTSALKELQGAHAEGK
                     ADFAEYASVDRNKKCRHSTNAESILGTSSDLDEETPPPVPVKLLDENANLPEKGEHGA
                     EEQAPEAPSGHSKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQSAHKPGPESTCQ
                     CPQGPPQRSSSSCNDLYATVKDFEKTPNSISMLPPARRPGEEPEPDYEAIQTLNREDD
                     KVPLGTNGHLVPKENDYESIGDLQQGRDVTRL"
     exon            206..257
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /inference="alignment:Splign:2.1.0"
     exon            258..354
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /inference="alignment:Splign:2.1.0"
     exon            355..941
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1001
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1353
                     /gene="Pag1"
                     /gene_synonym="Cbp"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagggaagcc ctctagaccc atgtgatctc ctgtgaagaa ccgagcccac ctgctggtca
       61 ccccagctcc caccatggga cctgcaggaa gcgccctgag cagcgggcag atgcagatgc
      121 agatggtgct gtggggaagt ctggctgcgg tggccatgtt cttcctcatc accttcctca
      181 tccttctgtg ctccagttgt gacagagaca agaagccacg gcagcatagt ggagaccatg
      241 agagcctgat gaatgtgcct tccgacaagg agatgttcag ccattcagcg accagcctga
      301 ccacagatgc actggccagc agcgaacaga atggggtgct caccaacggc gacattcttt
      361 cagaagacag cacaatgacc tgcatgcagc attatgagga agtccagacc tcagcttcag
      421 atctgctgga ctcccaggac agcacgggaa aggccaagtg ccaccagagc cgggaactgc
      481 ccagaatccc gcctgagaac gcagtggacg cgatgctcac cgccagggcc gcagatgggg
      541 actccgggcc cggcgtggag gggccctacg aggtgctcaa ggatagttcc tcccaggaga
      601 acatggtgga ggactgcttg tatgagacag tgaaggaaat caaggaggtg gcagacaaaa
      661 gccagggcgg caagtccaag tccacttctg ccctgaagga gcttcagggg gcccacgccg
      721 agggcaaggc cgactttgct gaatacgcct ctgtggacag aaacaaaaag tgccgccaca
      781 gcacaaatgc agagagcatt ctgggaacgt ccagtgacct ggatgaggaa accccaccgc
      841 ctgtcccagt taaacttctg gatgagaatg ccaaccttcc agagaaggga gagcacgggg
      901 cagaagagca agctccagaa gcacccagtg gacacagcaa gagatttagt tccttgtcat
      961 acaagtctcg agaagaagac ccaactctta cagaagagga gatctcagca atgtattctt
     1021 cagtgaataa gcctggacaa tcggcacaca aacctgggcc ggaatctacc tgccagtgcc
     1081 cgcagggtcc ccctcagagg tcatcatctt cctgtaacga cctctatgcc actgtgaaag
     1141 acttcgagaa aactcccaac agcatcagca tgctgccacc agcaaggagg cccggtgagg
     1201 aaccagagcc tgactatgaa gccatacaga ctctaaacag agaggacgac aaggtccctc
     1261 tggggaccaa tggccacctt gtccccaagg agaatgacta tgagagcatt ggggacttgc
     1321 agcaaggcag ggatgtcacc aggctctagc aac
//
