LOCUS       NM_001399539            2258 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus prolyl 4-hydroxylase subunit alpha 2 (P4ha2),
            transcript variant 2, mRNA.
ACCESSION   NM_001399539 XM_006246300
VERSION     NM_001399539.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Helaakoski,T., Annunen,P., Vuori,K., MacNeil,I.A., Pihlajaniemi,T.
            and Kivirikko,K.I.
  TITLE     Cloning, baculovirus expression, and characterization of a second
            mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an
            alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta
            subunit
  JOURNAL   Proc Natl Acad Sci U S A 92 (10), 4427-4431 (1995)
   PUBMED   7753822
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Jan 12, 2022 this sequence version replaced XM_006246300.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582424.1699423.1,
                                           SRR26360172.69932.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                JAXUCZ010000010.1  38743894-38743959
            67-197              JAXUCZ010000010.1  38744410-38744540
            198-297             JAXUCZ010000010.1  38752515-38752614
            298-394             JAXUCZ010000010.1  38753285-38753381
            395-546             JAXUCZ010000010.1  38753759-38753910
            547-684             JAXUCZ010000010.1  38756455-38756592
            685-924             JAXUCZ010000010.1  38759859-38760098
            925-1118            JAXUCZ010000010.1  38761482-38761675
            1119-1295           JAXUCZ010000010.1  38762418-38762594
            1296-1366           JAXUCZ010000010.1  38766215-38766285
            1367-1466           JAXUCZ010000010.1  38766506-38766605
            1467-1520           JAXUCZ010000010.1  38767357-38767410
            1521-1586           JAXUCZ010000010.1  38767938-38768003
            1587-1655           JAXUCZ010000010.1  38770202-38770270
            1656-1752           JAXUCZ010000010.1  38770582-38770678
            1753-2258           JAXUCZ010000010.1  38772236-38772741
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..2258
                     /gene="P4ha2"
                     /note="prolyl 4-hydroxylase subunit alpha 2"
                     /db_xref="GeneID:360526"
                     /db_xref="RGD:1309673"
     exon            1..66
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            67..197
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            198..297
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1823
                     /gene="P4ha2"
                     /EC_number="1.14.11.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 2; procollagen-proline, 2-oxoglutarate
                     4-dioxygenase (proline 4-hydroxylase), alpha polypeptide
                     II; procollagen-proline, 2-oxoglutarate 4-dioxygenase
                     (proline 4-hydroxylase), alpha II polypeptide; prolyl
                     4-hydroxylase, alpha polypeptide II"
                     /codon_start=1
                     /product="prolyl 4-hydroxylase subunit alpha-2 isoform 1
                     precursor"
                     /protein_id="NP_001386468.1"
                     /db_xref="GeneID:360526"
                     /db_xref="RGD:1309673"
                     /translation="MKLQVLVLLMSWFGVLSWVQAEFFTSIGHMTDLIYAEKDLVQSL
                     KEYILAEEAKLSKIKSWASKMEALTSKSAADPEGYLAHPVNAYKLVKRLNTDWPALGD
                     LVLQDAAAGFVANLSVQRQFFPTDEDESGAARALMRLQDTYKLDPDMISRGELPGTKY
                     QAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSYAVF
                     QLGDLHRAVELTRRLLSLDPSHERAGGNLRYFERLLEEERGKSLSNQTDAGLASQENL
                     YERPVDYLPERDVYESLCRGEGIKMTPRRQKRLFCRYHHGNRVPQLLIAPFKEEDEWD
                     SPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDD
                     PVVARVNRRMQHITGLTVKTAELLQVANYGMGGQYEPHFDFSRSDERDAFKRLGTGNR
                     VATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLV
                     GCKWVSNKWFHERGQEFLRPCGTTEVD"
     exon            298..394
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            395..546
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            547..684
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            685..924
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1118
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1295
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1366
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1466
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1520
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1586
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1655
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1752
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..2258
                     /gene="P4ha2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagccgaga gcgcccgggc gacgccgcgg gaggttctgg aaacgccggc tgctgcgcgt
       61 gtccagtagt ttcagagacc ggtggcgatt ggctgactga ttcaacaaat agagcattct
      121 ctgtgcctgg agactttcaa ggacggaggc aggcagaagg gaagactcag aaagtccgtg
      181 tccagagcac cggcaaggtc ccgccctttc cagtcatgaa gctccaggtg ttggtgttgc
      241 tgatgtcctg gtttggtgtc ctgagctggg tgcaggcgga attcttcacc tccattgggc
      301 acatgaccga cctgatttat gcagagaagg acctcgtcca gtctctgaag gagtacatcc
      361 tggcagagga agccaagctc tccaagatta agagctgggc cagtaagatg gaggccctga
      421 ccagcaagtc agctgctgac cccgagggct acctggccca tcctgtgaac gcctataagc
      481 tggtgaagcg attgaacaca gactggcctg ccctggggga ccttgtcctc caggacgcgg
      541 ctgcaggttt tgtggctaac ctctcagttc agcggcagtt cttccccact gacgaggacg
      601 agtctggagc tgccagagcc ctgatgcgac ttcaggacac ctacaaactg gatccggaca
      661 tgatttctag aggggagctt ccaggaacaa agtaccaggc catgctgagt gtggatgact
      721 gctttgggat gggccgctca gcttacaacg aaggagacta ttaccacacc gtgttgtgga
      781 tggagcaggt gctgaagcag ctggatgctg gggaggaggc cactgttacc aaatccctgg
      841 tgctggacta cctgagctat gccgtcttcc agctgggtga cctgcaccgt gcggtggaac
      901 tcacccgtcg cctgctctct cttgacccga gccatgaacg agccggaggg aacctgcggt
      961 actttgagcg gctgttagag gaagaaagag gaaaatcatt gtcgaatcag acggacgctg
     1021 gactagcatc gcaggaaaac ttgtacgaga ggcctgtaga ctacctgccc gagagggacg
     1081 tgtatgagag cctgtgtcga ggggagggca tcaaaatgac accccggagg cagaagaggc
     1141 ttttctgtag ataccaccat ggaaacagag tgccacagct cctcattgcc cccttcaaag
     1201 aggaagacga gtgggacagc ccacacatcg tcaggtacta tgatgtgatg tcggacgaag
     1261 agatcgagag gatcaaggag attgctaagc ccaaactcgc acgagccact gtgcgtgatc
     1321 ccaagacagg tgtcctcact gttgctagct acagagtttc caaaagctcc tggctagagg
     1381 aagacgatga ccctgttgtg gcccgagtca accgcaggat gcagcacatc acagggctga
     1441 cggtgaagac tgcagagcta ttgcaggtcg caaactacgg aatgggggga cagtacgaac
     1501 cacactttga cttctcgagg agcgatgagc gagatgcttt caagcgttta gggactggga
     1561 accgtgtggc cacatttcta aactacatga gtgatgttga agctggtggc gccactgtct
     1621 tccccgactt gggagctgcg atttggccca agaagggcac ggctgtattt tggtataacc
     1681 ttcttcggag cggggaaggc gattatcgaa cgaggcacgc ggcctgccct gtgcttgtgg
     1741 gctgcaagtg ggtctccaat aagtggttcc atgagcgagg acaggagttc ttgaggcctt
     1801 gtgggacaac ggaagtcgat tgacgtcctt ttctgctctg cccctccctg tcccacagtc
     1861 caaatcatct tcaagttcag tgtgacagct tcctttgtat gtcccagctc ctgtcaagca
     1921 ggccattggg ggagccagtg tttgtctgaa ttgagagagt gtgtcctgag ccgagtcctg
     1981 ggtgacctgg gccgcaggct ctggccagcc tatgcctgcc ctggctccca gggtagtctt
     2041 gtcatggctg tagtagagcc agactgtagc acccagcacg gtgcctttgt acctcagata
     2101 tttcaggtgg acgatgtttc agtgaaacca aagttctgac actgtttaca tgtttgtttt
     2161 ctatgacatt tctatttgtt gtggctttaa ccaaaaaata aaataaaaca aaacaaaaca
     2221 aaataaaatg ttcctgccag aagccttaaa gagcttta
//
