LOCUS       NM_172062               2145 bp    mRNA    linear   ROD 03-FEB-2024
DEFINITION  Rattus norvegicus prolyl 4-hydroxylase subunit alpha 1 (P4ha1),
            mRNA.
ACCESSION   NM_172062
VERSION     NM_172062.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Anantharajan,J., Koski,M.K., Kursula,P., Hieta,R., Bergmann,U.,
            Myllyharju,J. and Wierenga,R.K.
  TITLE     The structural motifs for substrate binding and dimerization of the
            alpha subunit of collagen prolyl 4-hydroxylase
  JOURNAL   Structure 21 (12), 2107-2118 (2013)
   PUBMED   24207127
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 2145)
  AUTHORS   Holster,T., Pakkanen,O., Soininen,R., Sormunen,R., Nokelainen,M.,
            Kivirikko,K.I. and Myllyharju,J.
  TITLE     Loss of assembly of the main basement membrane collagen, type IV,
            but not fibril-forming collagens and embryonic death in collagen
            prolyl 4-hydroxylase I null mice
  JOURNAL   J Biol Chem 282 (4), 2512-2519 (2007)
   PUBMED   17135260
REFERENCE   4  (bases 1 to 2145)
  AUTHORS   Takahashi,S., Dohi,N., Takahashi,Y. and Miura,T.
  TITLE     Cloning and characterization of the 5'-flanking region of the rat
            P4Halpha gene encoding the prolyl 4-hydroxylase alpha(I) subunit
  JOURNAL   Biochim Biophys Acta 1574 (3), 354-358 (2002)
   PUBMED   11997102
REFERENCE   5  (bases 1 to 2145)
  AUTHORS   Takahashi,Y., Takahashi,S., Shiga,Y., Yoshimi,T. and Miura,T.
  TITLE     Hypoxic induction of prolyl 4-hydroxylase alpha (I) in cultured
            cells
  JOURNAL   J Biol Chem 275 (19), 14139-14146 (2000)
   PUBMED   10799490
REFERENCE   6  (bases 1 to 2145)
  AUTHORS   Helaakoski,T., Annunen,P., Vuori,K., MacNeil,I.A., Pihlajaniemi,T.
            and Kivirikko,K.I.
  TITLE     Cloning, baculovirus expression, and characterization of a second
            mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an
            alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta
            subunit
  JOURNAL   Proc Natl Acad Sci U S A 92 (10), 4427-4431 (1995)
   PUBMED   7753822
REFERENCE   7  (bases 1 to 2145)
  AUTHORS   Hopkinson,I., Smith,S.A., Donne,A., Gregory,H., Franklin,T.J.,
            Grant,M.E. and Rosamond,J.
  TITLE     The complete cDNA derived sequence of the rat prolyl 4-hydroxylase
            alpha subunit
  JOURNAL   Gene 149 (2), 391-392 (1994)
   PUBMED   7959029
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC078703.1.
            
            [WARNING] On Mar 18, 2024 this sequence was replaced by
            NM_172062.3.
            
            On Aug 8, 2004 this sequence version replaced NM_172062.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078703.1, X78949.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20q11"
     gene            1..2145
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /note="prolyl 4-hydroxylase subunit alpha 1"
                     /db_xref="GeneID:64475"
                     /db_xref="RGD:621000"
     exon            1..208
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            209..311
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     CDS             236..1840
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /EC_number="1.14.11.2"
                     /note="4-PH alpha-1;
                     procollagen-proline,2-oxoglutarate-4-dioxygenase subunit
                     alpha-1; prolyl 4-hydroxylase alpha subunit;
                     procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline
                     4-hydroxylase), alpha polypeptide I; procollagen-proline,
                     2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase),
                     alpha 1 polypeptide; prolyl 4-hydroxylase, alpha
                     polypeptide I"
                     /codon_start=1
                     /product="prolyl 4-hydroxylase subunit alpha-1 precursor"
                     /protein_id="NP_742059.2"
                     /db_xref="GeneID:64475"
                     /db_xref="RGD:621000"
                     /translation="MIWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKD
                     YIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLI
                     LKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKS
                     FLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQ
                     GDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKK
                     GIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDK
                     PRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDP
                     VVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRI
                     ATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVG
                     NKWVSNKWLHERGQEFRRPCTLSELE"
     exon            312..408
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            409..560
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            561..698
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            699..938
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1135
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1312
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1383
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1483
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1537
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1603
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1672
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1769
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..2139
                     /gene="P4ha1"
                     /gene_synonym="PHalphaI"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgactgagc gcacgtagcg agagctgtgg ccgccccgtt ctcggcgcgc cggcgcggct
       61 ggggactata aaaggctcct ctggctccct ctgctgccca gtcgcaccgc cagcaggctg
      121 agcgcggcag ggagctacct agtggggact acaggcgaca ggaccgaccc gagccgagct
      181 gtcgccttcc tcacggcccg ctatccaggt gtgtgaacct gtggggtgct ccaagatgat
      241 ctggggtgta ttaatgatgg ggattctact tcctcagtgt tcagcccatc caggcttttt
      301 tacttcaatt ggtcagatga ctgacttgat ccataatgag aaagacctgg tgacgtcact
      361 aaaagattac attaaagcag aagaggacaa gttagagcaa atcaaaaaat gggcagagaa
      421 gttagaccgg ctaacaagta cagcaacaaa agatccagaa gggtttgtcg gacaccctgt
      481 aaatgcattc aagttaatga aacgtctgaa caccgagtgg agtgagttgg agaatctgat
      541 cctcaaggat atgtcagatg gcttcatctc taacctgacc attcagaggc agtacttccc
      601 taacgacgaa gaccaggttg gggctgcaaa agctttgttt cgtctgcaag acacctacaa
      661 cctagacacg aataccatct cgaagggcaa tcttccagga gtgaaacaca agtcttttct
      721 aacagctgag gactgctttg agttgggcaa agtggcctat acagaagcag attattacca
      781 cacagaactc tggatggagc aggctctgat gcagctggag gagggagaga tgtctactgt
      841 agacaaagtc tcggttctag attatttgag ctatgcagtg taccagcagg gtgacctgga
      901 taaggcactt ctgcttacaa agaaacttct tgaactagat cctgaacacc agagagccaa
      961 tggtaactta gtatattttg agtatataat gagtaaagaa aaagatgcca ataagtctgc
     1021 ttcgggtgac cagtctgatc agaaaactac accaaagaaa aaggggattg ctgtggacta
     1081 cctgccagag agacagaagt acgaaatgct gtgccgtggg gagggtatca aaatgactcc
     1141 tcggagacaa aaaaggctgt tctgccgcta ccatgatgga aaccggaatc ctaaatttat
     1201 cctggcccca gccaagcagg aggatgagtg ggacaagcct cgcatcattc gtttccatga
     1261 catcatctca gatgccgaga ttgagatcgt caaagattta gcaaagccca ggctgagccg
     1321 agctacagta catgaccctg agactgggaa attgaccaca gcacagtaca gagtatctaa
     1381 gagtgcttgg ctgtctggct atgaagatcc tgtggtgtct cgaattaata tgagaataca
     1441 agatctcaca ggactggatg tttccacggc agaggaatta caggtagcaa attatggagt
     1501 tggaggacag tatgaacccc attttgactt tgccaggaaa gacgagccgg atgcttttag
     1561 agagcttggg acaggaaata ggattgccac gtggctcttc tacatgagtg atgtgtctgc
     1621 tggaggcgct actgtttttc ctgaagtggg agccagtgtt tggcccaaaa aaggcactgc
     1681 tgtcttctgg tacaatctgt ttgccagtgg agaaggagat tacagtacac ggcacgcagc
     1741 ctgtcctgtg ctagtgggaa acaaatgggt atccaacaaa tggctccatg aacgtggaca
     1801 ggaatttcga aggccgtgta ccctgtcaga attggaatga caaccaggct tcccgtggct
     1861 cctctcgtcc tctaacgcac caggcatgat cgctgactgt aacattcaga agtttacagc
     1921 tgactaacac tccatgatta attcggccgt gaaccccatc ccatgtttca tctgtggaca
     1981 atcacttatt tttgtgaatt tttttaaatt aacactcagt caccacattg tgtaaaactt
     2041 atggttcagt tatcacagga tacaggatat ttaaaatgag gaacctagtt ctgttgttta
     2101 aaagagcttt ttggttgatt taaaaaaaaa aaaaaaaaaa aaaaa
//
