LOCUS       NM_053656               1833 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus purinergic receptor P2X 2 (P2rx2), mRNA.
ACCESSION   NM_053656
VERSION     NM_053656.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Teixeira,J.M., Parada,C.A. and Tambeli,C.H.
  TITLE     P2X3 and P2X2/3 receptors activation induces articular hyperalgesia
            by an indirect sensitization of the primary afferent nociceptor in
            the rats' knee joint
  JOURNAL   Eur J Pharmacol 879, 173054 (2020)
   PUBMED   32145326
  REMARK    GeneRIF: P2X3 and P2X2/3 receptors activation induces articular
            hyperalgesia by an indirect sensitization of the primary afferent
            nociceptor in the rats' knee joint.
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Zhang,Y.L., Liu,Y.G., Chen,D.J., Yang,B.L., Liu,T.T., Li,J.J.,
            Wang,X.Q., Li,H.R. and Liu,Z.X.
  TITLE     Microencapsulated Schwann cell transplantation inhibits P2X2/3
            receptors overexpression in a sciatic nerve injury rat model with
            neuropathic pain
  JOURNAL   Neurosci Lett 676, 51-57 (2018)
   PUBMED   29608947
  REMARK    GeneRIF: Study showed that in a rat model of neuropathic pain,
            Schwann cell and microencapsulated-Schwann cell transplantation
            reduce P2X2/3 receptor expression in L4-5 dorsal root ganlia.
REFERENCE   3  (bases 1 to 1833)
  AUTHORS   Emerit,M.B., Baranowski,C., Diaz,J., Martinez,A., Areias,J.,
            Alterio,J., Masson,J., Boue-Grabot,E. and Darmon,M.
  TITLE     A New Mechanism of Receptor Targeting by Interaction between Two
            Classes of Ligand-Gated Ion Channels
  JOURNAL   J Neurosci 36 (5), 1456-1470 (2016)
   PUBMED   26843630
  REMARK    GeneRIF: A trafficking mechanism for 5-HT3A receptor depends on a
            functional interaction with P2X2 receptor.
REFERENCE   4  (bases 1 to 1833)
  AUTHORS   Habermacher,C., Martz,A., Calimet,N., Lemoine,D., Peverini,L.,
            Specht,A., Cecchini,M. and Grutter,T.
  TITLE     Photo-switchable tweezers illuminate pore-opening motions of an
            ATP-gated P2X ion channel
  JOURNAL   Elife 5, e11050 (2016)
   PUBMED   26808983
  REMARK    GeneRIF: These data further reveal that the inner and outer ends of
            adjacent pore-lining helices come closer during opening, likely
            through a hinge-bending motion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1833)
  AUTHORS   Liu,Y., Tian,X., Wu,Y., Chen,L., Yi,C.L., Li,Z.W., Zhang,Y. and
            Li,C.Y.
  TITLE     Phenotypes of ATP-activated current associated with their genotypes
            of P2X1-6 subunits in neurons innervating tooth-pulp
  JOURNAL   Biochem Biophys Res Commun 458 (3), 596-600 (2015)
   PUBMED   25680470
  REMARK    GeneRIF: The study explores the association of the phenotype of
            ATP-activated current with the genotype of P2X1-6 subunits in
            neurons innervating tooth-pulp.
REFERENCE   6  (bases 1 to 1833)
  AUTHORS   Troyanovskaya,M. and Wackym,P.A.
  TITLE     Evidence for three additional P2X2 purinoceptor isoforms produced
            by alternative splicing in the adult rat vestibular end-organs
  JOURNAL   Hear Res 126 (1-2), 201-209 (1998)
   PUBMED   9872146
REFERENCE   7  (bases 1 to 1833)
  AUTHORS   Salih,S.G., Housley,G.D., Burton,L.D. and Greenwood,D.
  TITLE     P2X2 receptor subunit expression in a subpopulation of cochlear
            type I spiral ganglion neurones
  JOURNAL   Neuroreport 9 (2), 279-282 (1998)
   PUBMED   9507969
REFERENCE   8  (bases 1 to 1833)
  AUTHORS   Brandle,U., Spielmanns,P., Osteroth,R., Sim,J., Surprenant,A.,
            Buell,G., Ruppersberg,J.P., Plinkert,P.K., Zenner,H.P. and
            Glowatzki,E.
  TITLE     Desensitization of the P2X(2) receptor controlled by alternative
            splicing
  JOURNAL   FEBS Lett 404 (2-3), 294-298 (1997)
   PUBMED   9119082
REFERENCE   9  (bases 1 to 1833)
  AUTHORS   Housley,G.D., Greenwood,D., Bennett,T. and Ryan,A.F.
  TITLE     Identification of a short form of the P2xR1-purinoceptor subunit
            produced by alternative splicing in the pituitary and cochlea
  JOURNAL   Biochem Biophys Res Commun 212 (2), 501-508 (1995)
   PUBMED   7542879
REFERENCE   10 (bases 1 to 1833)
  AUTHORS   Brake,A.J., Wagenbach,M.J. and Julius,D.
  TITLE     New structural motif for ligand-gated ion channels defined by an
            ionotropic ATP receptor
  JOURNAL   Nature 371 (6497), 519-523 (1994)
   PUBMED   7523952
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Nov 27, 2020 this sequence version replaced NM_053656.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U14414.1, SRR26643291.33155.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               JAXUCZ010000012.1  51999433-51999605
            174-309             JAXUCZ010000012.1  51999749-51999884
            310-381             JAXUCZ010000012.1  51999982-52000053
            382-457             JAXUCZ010000012.1  52000133-52000208
            458-554             JAXUCZ010000012.1  52000287-52000383
            555-635             JAXUCZ010000012.1  52000537-52000617
            636-774             JAXUCZ010000012.1  52000719-52000857
            775-905             JAXUCZ010000012.1  52001180-52001310
            906-999             JAXUCZ010000012.1  52001474-52001567
            1000-1065           JAXUCZ010000012.1  52001709-52001774
            1066-1833           JAXUCZ010000012.1  52001860-52002627
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..1833
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /note="purinergic receptor P2X 2"
                     /db_xref="GeneID:114115"
                     /db_xref="RGD:620251"
     exon            1..173
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..1455
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /note="ATP receptor; purinergic receptor P2X, ligand-gated
                     ion channel, 2"
                     /codon_start=1
                     /product="P2X purinoceptor 2"
                     /protein_id="NP_446108.2"
                     /db_xref="GeneID:114115"
                     /db_xref="RGD:620251"
                     /translation="MVRRLARGCWSAFWDYETPKVIVVRNRRLGFVHRMVQLLILLYF
                     VWYVFIVQKSYQDSETGPESSIITKVKGITMSEDKVWDVEEYVKPPEGGSVVSIITRI
                     EVTPSQTLGTCPESMRVHSSTCHSDDDCIAGQLDMQGNGIRTGHCVPYYHGDSKTCEV
                     SAWCPVEDGTSDNHFLGKMAPNFTILIKNSIHYPKFKFSKGNIASQKSDYLKHCTFDQ
                     DSDPYCPIFRLGFIVEKAGENFTELAHKGGVIGVIINWNCDLDLSESECNPKYSFRRL
                     DPKYDPASSGYNFRFAKYYKINGTTTTRTLIKAYGIRIDVIVHGQAGKFSLIPTIINL
                     ATALTSIGVGSFLCDWILLTFMNKNKLYSHKKFDKVRTPKHPSSRWPVTLALVLGQIP
                     PPPSHYSQDQPPSPPSGEGPTLGEGAELPLAVQSPRPCSISALTEQVVDTLGQHMGQR
                     PPVPEPSQQDSTSTDPKGLAQL"
     exon            174..309
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            310..381
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            382..457
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            458..554
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            555..635
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            636..774
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            775..905
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            906..999
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1065
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1833
                     /gene="P2rx2"
                     /gene_synonym="P2X2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgctgcac agccccggct tcccgcgggg gcggccatgg tccggcgctt ggcccggggc
       61 tgctggtccg cgttctggga ctacgagacg cctaaggtga tcgtggtgcg gaatcggcgc
      121 ctgggattcg tgcaccgcat ggtgcagctt ctcatcctgc tttacttcgt gtggtacgtc
      181 ttcatcgtgc agaaaagcta ccaggacagc gagaccggac cggagagctc catcatcacc
      241 aaagtcaagg ggatcaccat gtcggaagac aaagtgtggg acgtggagga atacgtaaag
      301 cccccggagg ggggcagtgt agtcagcatc atcaccagga tcgaggttac cccttcccag
      361 accttgggaa catgcccaga gagcatgagg gttcacagct ctacctgcca ttcagacgac
      421 gactgtattg ccggacagct ggacatgcaa ggcaatggga ttcgcacagg gcactgtgta
      481 ccctattacc atggggactc caagacctgc gaggtgtcag cctggtgccc ggtggaggat
      541 ggaacttctg acaaccattt tctgggtaaa atggccccaa atttcaccat cctcatcaag
      601 aacagcatcc actaccccaa gttcaagttc tcaaagggca acattgcaag ccagaagagt
      661 gactacctca agcattgcac atttgatcag gactctgacc catactgtcc catcttcagg
      721 ctgggtttca ttgttgagaa ggcaggagag aacttcacag aactggcaca caagggcggt
      781 gtcattggag tcatcatcaa ctggaactgt gacctggact tgtctgaatc agagtgcaac
      841 cccaaatatt ctttccggag gctcgacccc aagtatgacc ctgcctcctc aggctacaac
      901 ttcaggtttg ccaagtatta caagataaac ggcactacca ccactcgaac tctcatcaaa
      961 gcctatggga ttcgaatcga tgttatcgtg catggacagg cagggaaatt cagtctcatt
     1021 cccaccatca tcaatctggc cactgctctg acctccatcg gggtgggctc cttcctgtgt
     1081 gactggattt tgttaacgtt catgaacaaa aacaagctct acagccataa gaagttcgac
     1141 aaggtgcgta ctccaaagca tccctcaagt agatggcctg tgacccttgc ccttgtcttg
     1201 ggccagatcc ctcccccacc tagtcactac tcccaggatc agccacccag ccctccatca
     1261 ggtgaaggac caactttggg agaaggggca gagctaccac tggctgtcca gtctcctcgg
     1321 ccttgctcca tctctgctct gactgagcag gtggtggaca cacttggcca gcatatggga
     1381 caaagacctc ctgtccctga gccttcccaa caggactcca catccacgga ccccaaaggt
     1441 ttggcccaac tttgatctcg tcctcactaa actacagacc tggacctggg aaggcagaga
     1501 cagctttggc tgctaaggca gtcctagaga agatctgcgc tcttcagtaa ccatgtccat
     1561 gtgactggga aacagaaacc tgtgcaagag gacaggcgtc ttgctttagc ccaagcttac
     1621 attcttcctc tccctaaggc ctctggggag aagtgggttc cctgccatct cctttcccaa
     1681 cagaactcct cataggacct ttccctgctc acctcttgta ctctcataca gtattcaggg
     1741 accccaagtt aggggctatg ccctcctgtt gtataatttc aagcccccct ttagaagttg
     1801 cagcatgctg agttcaataa accagtgatg agc
//
