LOCUS       NM_001401354            3111 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus OTU deubiquitinase, ubiquitin aldehyde binding 2
            (Otub2), transcript variant 1, mRNA.
ACCESSION   NM_001401354 XM_006240477
VERSION     NM_001401354.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Mevissen,T.E., Hospenthal,M.K., Geurink,P.P., Elliott,P.R.,
            Akutsu,M., Arnaudo,N., Ekkebus,R., Kulathu,Y., Wauer,T., El
            Oualid,F., Freund,S.M., Ovaa,H. and Komander,D.
  TITLE     OTU deubiquitinases reveal mechanisms of linkage specificity and
            enable ubiquitin chain restriction analysis
  JOURNAL   Cell 154 (1), 169-184 (2013)
   PUBMED   23827681
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   3  (bases 1 to 3111)
  AUTHORS   Edelmann,M.J., Iphofer,A., Akutsu,M., Altun,M., di Gleria,K.,
            Kramer,H.B., Fiebiger,E., Dhe-Paganon,S. and Kessler,B.M.
  TITLE     Structural basis and specificity of human otubain 1-mediated
            deubiquitination
  JOURNAL   Biochem J 418 (2), 379-390 (2009)
   PUBMED   18954305
REFERENCE   4  (bases 1 to 3111)
  AUTHORS   Nanao,M.H., Tcherniuk,S.O., Chroboczek,J., Dideberg,O., Dessen,A.
            and Balakirev,M.Y.
  TITLE     Crystal structure of human otubain 2
  JOURNAL   EMBO Rep 5 (8), 783-788 (2004)
   PUBMED   15258613
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Feb 9, 2022 this sequence version replaced XM_006240477.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643294.26564.1,
                                           SRR21887623.2725132.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               JAXUCZ010000006.1  128309863-128310062
            201-518             JAXUCZ010000006.1  128314891-128315208
            519-614             JAXUCZ010000006.1  128322183-128322278
            615-733             JAXUCZ010000006.1  128322921-128323039
            734-818             JAXUCZ010000006.1  128324956-128325040
            819-1013            JAXUCZ010000006.1  128325373-128325567
            1014-3111           JAXUCZ010000006.1  128326347-128328444
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q32"
     gene            1..3111
                     /gene="Otub2"
                     /note="OTU deubiquitinase, ubiquitin aldehyde binding 2"
                     /db_xref="GeneID:314405"
                     /db_xref="RGD:1306688"
     exon            1..200
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..1220
                     /gene="Otub2"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin thioesterase OTUB2; OTU domain, ubiquitin
                     aldehyde binding 2"
                     /codon_start=1
                     /product="ubiquitin thioesterase OTUB2 isoform 1"
                     /protein_id="NP_001388283.1"
                     /db_xref="GeneID:314405"
                     /db_xref="RGD:1306688"
                     /translation="MTWGKDTLRPRSLTLEEMALPSSLQDFTPHFHFRLHPHGLFCAS
                     SCRRSRTESSGKTPTLRSALRPVLPTPKSPFVVCSQLRRLLQHPPGKCPGCSHSPAPK
                     PSHLQSETSFNLISEKCDILSILRDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYR
                     ALGYSYLESLLGKGREILKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKD
                     SSVSSLLKVFNDQSSSDRIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEP
                     MAMECDHVQITALSQALNIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNIL
                     YAAEKH"
     exon            201..518
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
     exon            519..614
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..733
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
     exon            734..818
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
     exon            819..1013
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..3111
                     /gene="Otub2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aatcgcgggc cgtgcgtctt gctgggacac agtggaggtc tgcgctccgg tctgaggggc
       61 ggtcgggtgt attctgcact caccccacac ccgcgaggcc cgcgccgccc gacccaacgg
      121 cggagccgcc cagcgcctcc cctggcaccc gctcgctcct cgctggagcg agacgtggcc
      181 cgacggctcc ggtcactatg acctggggca aggacaccct gcggccacgt tctttgacac
      241 ttgaggagat ggcccttcct tccagtctcc aggacttcac tccccacttc cacttcaggc
      301 tacatcctca cggccttttc tgtgccagct cctgccggcg ttctcgcaca gaatcttctg
      361 ggaagacccc cactttacgg tccgctctcc gtcctgtctt acccacacca aaatcgccct
      421 tcgtggtctg ttcacagctt cgaaggttgc tccagcatcc gcctgggaag tgtcccggct
      481 gtagccactc cccggccccg aagccttcac atctgcagag tgaaacatct ttcaacctaa
      541 tatcagaaaa atgtgacatt ctatctattc ttcgggatca tcctgaaaac aggatttacc
      601 agaggaaaat ccaggaactc agcaaaagat tcacctcaat ccgaaagacc aaaggagacg
      661 ggaactgctt ctacagggcc ttgggctatt cctacctgga gtctttgctg ggcaagggca
      721 gagagatcct caagttcaaa gagcgtgtgc tacagacccc aaacgacctt ttggctgctg
      781 gctttgagga acataagttc agaaacttct ttaatgcttt ttacagtgtg gtagagctgg
      841 tagagaagga cagctcagtg tccagcctgc tgaaggtgtt caacgaccag agctcctcgg
      901 atcgaatagt gcagttctta cgcctcctca catcagcctt catcaggaac cgagctgact
      961 tcttccgaca tttcatcgat gaggagatgg acatcaaaga cttctgcact cacgaagtag
     1021 agcccatggc catggagtgt gaccatgtgc agatcacagc cctgtcgcag gccctcaaca
     1081 tcgctctgca ggtggagtat gtggatgaga tggacacagc tctgaaccac cacgtgttcc
     1141 ccgaggctgc caccccttcc gtttatctgc tctataaaac atcccactac aacatccttt
     1201 atgcagctga aaaacactga ttattaggcc atgccagagc ctgtcatcta atggactgca
     1261 ctctgaatgg aacatttctg ccttggcaac tttttaagtg atttagacaa ctaaaaaaca
     1321 tatagccttt tgggtaaagc cactgaactg agacctgtgc ccactatgga ctgtgatgac
     1381 ttaataggaa ttttagtatt tattttgata gatatataat gcctgctaac tctggaccag
     1441 aaggccagaa accctgtctc tgtacccatt tacttcaaga acctcgttgg acctatttat
     1501 ctggcagagg ttcctgacag tgctgatatg ctgctgtgtg aaaatgggga tatcgggctt
     1561 gtctcaccca gggcagtggc cctcataggg aagacaagct taggccagct gtcgtgtaga
     1621 ggtgacttac cagacctatg aagagtccag cccggctatg tggatgggtc atggcgaact
     1681 gtctgaatgt gagggtgtgt atactttcgt tcatggtggt atacttgcct atccactgaa
     1741 ggagagagaa aaaagactta ccaccgtcca gagaaggaca ttagccttca gcatgacgct
     1801 ccggttgtga ggtcctctac ctcaggacca gaactcctga ctctggagga caggcccttc
     1861 agggttctgt caaccccagc tgaataaggc ttgctgagga caatggcttg tgcctaattc
     1921 agtgggtcca tgtctgggac caaagggcta tggctaccca tcctccccag tgaattctag
     1981 gagtagggcc taggtgatcg gctgggtatg tgcagcacag aacctagaag ggctgaggac
     2041 aggtttccca aggatctctg gttttgtcca ggccagcttc ataggcagct gggcagggaa
     2101 ggacaaacaa gccatagaca tgggcagcag ggatgtttcc tcctgtgtgt gtacccctta
     2161 atagcccctt gccttgcctt gctgcccgaa gacatttagg agtccacctg aggacccacc
     2221 cagctgttgc tagccacacc ctcctatgta ggacaaccat gggacaacct ttccttggtt
     2281 acagggcttc cctcctgctg cactctgcag gaattagaca attggtgcta ctgtgagggt
     2341 ctgctgaaag cctgcacaca gggctgggcg caggatcttg acctgagtct acacagctta
     2401 gacacctgtc cttttgttcg aggaaagctg aaagcaccta gatgtttgtg cagtgtggga
     2461 cctctgccaa catttagacc caaaggctcc agctaagtga gtctatcgct ggcacaggag
     2521 tgtaaatctg cccttcatct tttgatgtgt tcagtgttgc agtctgatgt gggtacctct
     2581 gacaggtacc ctacaaaggt gggcacctat ccccatgact gtcttcagcc attagtctcc
     2641 acttccatta atctccactt ccaggcttaa tggtgtgagc acaggaaacc tgactgatgg
     2701 atgcgagttc caatgggtct ggggacaggg gatgggcttg ggaaaacgct aaaacctggc
     2761 tgagcatagc aaggctaggt tacagcacaa gtctgccagc tcttggtaga ggaggcaggg
     2821 gagcgcaggt gctgagttca tcctcgaaat gcttccaatt cctcctctcc cagagcagcc
     2881 agtggggcag cgcagagtgg ggcacctgct ctccgccctc tgagagcaga cggaagtgct
     2941 ggcaccagct gcccacgttc aactgtgtga tcttggccaa accctttcag ctctgaagca
     3001 aagaagcatg gcacctacaa gtgaaccggg tctaagggct tgggttcagt cagcttccct
     3061 taagtgaaag catcttccta ccattaaaac tgtttctatg aacaactttc a
//
