LOCUS       NM_001276720            7118 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus otoferlin (Otof), mRNA.
ACCESSION   NM_001276720 XM_001062291 XM_001065408 XM_002726716 XM_002729613
            XM_003750118 XM_003754154
VERSION     NM_001276720.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Vogl,C., Panou,I., Yamanbaeva,G., Wichmann,C., Mangosing,S.J.,
            Vilardi,F., Indzhykulian,A.A., Pangrsic,T., Santarelli,R.,
            Rodriguez-Ballesteros,M., Weber,T., Jung,S., Cardenas,E., Wu,X.,
            Wojcik,S.M., Kwan,K.Y., Del Castillo,I., Schwappach,B.,
            Strenzke,N., Corey,D.P., Lin,S.Y. and Moser,T.
  TITLE     Tryptophan-rich basic protein (WRB) mediates insertion of the
            tail-anchored protein otoferlin and is required for hair cell
            exocytosis and hearing
  JOURNAL   EMBO J 35 (23), 2536-2552 (2016)
   PUBMED   27458190
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Wu,W., Rahman,M.N., Guo,J., Roy,N., Xue,L., Cahill,C.M., Zhang,S.
            and Jia,Z.
  TITLE     Function coupling of otoferlin with GAD65 acts to modulate
            GABAergic activity
  JOURNAL   J Mol Cell Biol 7 (2), 168-179 (2015)
   PUBMED   25701657
  REMARK    GeneRIF: otoferlin, through GAD65, modulates GABAergic activity.
REFERENCE   3  (bases 1 to 7118)
  AUTHORS   Vogl,C., Cooper,B.H., Neef,J., Wojcik,S.M., Reim,K., Reisinger,E.,
            Brose,N., Rhee,J.S., Moser,T. and Wichmann,C.
  TITLE     Unconventional molecular regulation of synaptic vesicle
            replenishment in cochlear inner hair cells
  JOURNAL   J Cell Sci 128 (4), 638-644 (2015)
   PUBMED   25609709
REFERENCE   4  (bases 1 to 7118)
  AUTHORS   Ramakrishnan,N.A., Drescher,M.J., Morley,B.J., Kelley,P.M. and
            Drescher,D.G.
  TITLE     Calcium regulates molecular interactions of otoferlin with soluble
            NSF attachment protein receptor (SNARE) proteins required for hair
            cell exocytosis
  JOURNAL   J Biol Chem 289 (13), 8750-8766 (2014)
   PUBMED   24478316
  REMARK    GeneRIF: a direct role for otoferlin in exocytosis and modulation
            of calcium-dependent membrane fusion
REFERENCE   5  (bases 1 to 7118)
  AUTHORS   Duncker,S.V., Franz,C., Kuhn,S., Schulte,U., Campanelli,D.,
            Brandt,N., Hirt,B., Fakler,B., Blin,N., Ruth,P., Engel,J.,
            Marcotti,W., Zimmermann,U. and Knipper,M.
  TITLE     Otoferlin couples to clathrin-mediated endocytosis in mature
            cochlear inner hair cells
  JOURNAL   J Neurosci 33 (22), 9508-9519 (2013)
   PUBMED   23719817
  REMARK    GeneRIF: otoferlin-AP-2 interaction drives Ca(2+)- and
            stimulus-dependent compensating clathrin-mediated endocytosis in
            mature inner hair
REFERENCE   6  (bases 1 to 7118)
  AUTHORS   Tekin,M., Akcayoz,D. and Incesulu,A.
  TITLE     A novel missense mutation in a C2 domain of OTOF results in
            autosomal recessive auditory neuropathy
  JOURNAL   Am J Med Genet A 138 (1), 6-10 (2005)
   PUBMED   16097006
REFERENCE   7  (bases 1 to 7118)
  AUTHORS   Rodriguez-Ballesteros,M., del Castillo,F.J., Martin,Y.,
            Moreno-Pelayo,M.A., Morera,C., Prieto,F., Marco,J., Morant,A.,
            Gallo-Teran,J., Morales-Angulo,C., Navas,C., Trinidad,G.,
            Tapia,M.C., Moreno,F. and del Castillo,I.
  TITLE     Auditory neuropathy in patients carrying mutations in the otoferlin
            gene (OTOF)
  JOURNAL   Hum Mutat 22 (6), 451-456 (2003)
   PUBMED   14635104
REFERENCE   8  (bases 1 to 7118)
  AUTHORS   Migliosi,V., Modamio-Hoybjor,S., Moreno-Pelayo,M.A.,
            Rodriguez-Ballesteros,M., Villamar,M., Telleria,D., Menendez,I.,
            Moreno,F. and Del Castillo,I.
  TITLE     Q829X, a novel mutation in the gene encoding otoferlin (OTOF), is
            frequently found in Spanish patients with prelingual non-syndromic
            hearing loss
  JOURNAL   J Med Genet 39 (7), 502-506 (2002)
   PUBMED   12114484
REFERENCE   9  (bases 1 to 7118)
  AUTHORS   Yasunaga,S., Grati,M., Chardenoux,S., Smith,T.N., Friedman,T.B.,
            Lalwani,A.K., Wilcox,E.R. and Petit,C.
  TITLE     OTOF encodes multiple long and short isoforms: genetic evidence
            that the long ones underlie recessive deafness DFNB9
  JOURNAL   Am J Hum Genet 67 (3), 591-600 (2000)
   PUBMED   10903124
REFERENCE   10 (bases 1 to 7118)
  AUTHORS   Yasunaga,S., Grati,M., Cohen-Salmon,M., El-Amraoui,A., Mustapha,M.,
            Salem,N., El-Zir,E., Loiselet,J. and Petit,C.
  TITLE     A mutation in OTOF, encoding otoferlin, a FER-1-like protein,
            causes DFNB9, a nonsyndromic form of deafness
  JOURNAL   Nat Genet 21 (4), 363-369 (1999)
   PUBMED   10192385
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On or before Mar 1, 2013 this sequence version replaced
            XM_002729613.2, XM_001062291.3, XM_003750118.1, XM_001065408.3,
            XM_002726716.2, XM_003754154.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760393,
                              SAMEA5760433 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               JAXUCZ010000006.1  31647914-31648101
            189-247             JAXUCZ010000006.1  31668946-31669004
            248-336             JAXUCZ010000006.1  31685195-31685283
            337-436             JAXUCZ010000006.1  31691458-31691557
            437-618             JAXUCZ010000006.1  31693006-31693187
            619-692             JAXUCZ010000006.1  31706209-31706282
            693-819             JAXUCZ010000006.1  31707492-31707618
            820-874             JAXUCZ010000006.1  31708084-31708138
            875-1006            JAXUCZ010000006.1  31713380-31713511
            1007-1069           JAXUCZ010000006.1  31717506-31717568
            1070-1154           JAXUCZ010000006.1  31718005-31718089
            1155-1314           JAXUCZ010000006.1  31720469-31720628
            1315-1501           JAXUCZ010000006.1  31722097-31722283
            1502-1688           JAXUCZ010000006.1  31722446-31722632
            1689-1912           JAXUCZ010000006.1  31724336-31724559
            1913-2021           JAXUCZ010000006.1  31724971-31725079
            2022-2202           JAXUCZ010000006.1  31725532-31725712
            2203-2323           JAXUCZ010000006.1  31725813-31725933
            2324-2424           JAXUCZ010000006.1  31726376-31726476
            2425-2515           JAXUCZ010000006.1  31726578-31726668
            2516-2632           JAXUCZ010000006.1  31726782-31726898
            2633-2785           JAXUCZ010000006.1  31727171-31727323
            2786-2975           JAXUCZ010000006.1  31727601-31727790
            2976-3100           JAXUCZ010000006.1  31727892-31728016
            3101-3235           JAXUCZ010000006.1  31728358-31728492
            3236-3397           JAXUCZ010000006.1  31728956-31729117
            3398-3517           JAXUCZ010000006.1  31729278-31729397
            3518-3679           JAXUCZ010000006.1  31729884-31730045
            3680-3842           JAXUCZ010000006.1  31730170-31730332
            3843-3973           JAXUCZ010000006.1  31730864-31730994
            3974-4003           JAXUCZ010000006.1  31732057-31732086
            4004-4135           JAXUCZ010000006.1  31732355-31732486
            4136-4202           JAXUCZ010000006.1  31732857-31732923
            4203-4339           JAXUCZ010000006.1  31734337-31734473
            4340-4474           JAXUCZ010000006.1  31734570-31734704
            4475-4612           JAXUCZ010000006.1  31734894-31735031
            4613-4740           JAXUCZ010000006.1  31735307-31735434
            4741-4911           JAXUCZ010000006.1  31735538-31735708
            4912-5072           JAXUCZ010000006.1  31736319-31736479
            5073-5215           JAXUCZ010000006.1  31737157-31737299
            5216-5304           JAXUCZ010000006.1  31737608-31737696
            5305-5403           JAXUCZ010000006.1  31739435-31739533
            5404-5645           JAXUCZ010000006.1  31739674-31739915
            5646-5824           JAXUCZ010000006.1  31740354-31740532
            5825-5925           JAXUCZ010000006.1  31740632-31740732
            5926-6128           JAXUCZ010000006.1  31741123-31741325
            6129-7118           JAXUCZ010000006.1  31743487-31744476
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q14"
     gene            1..7118
                     /gene="Otof"
                     /note="otoferlin"
                     /db_xref="GeneID:84573"
                     /db_xref="RGD:620646"
     exon            1..188
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..6106
                     /gene="Otof"
                     /note="fer-1-like protein 2"
                     /codon_start=1
                     /product="otoferlin"
                     /protein_id="NP_001263649.1"
                     /db_xref="GeneID:84573"
                     /db_xref="RGD:620646"
                     /translation="MALIVHLKTVSELRGRADRIAKVTFRGQSFYSRVLENCEDVADF
                     DETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVEVSDTLID
                     DNNAIIKTSLSMEVRYQAADGTVGPWDDGDFLGDESLQEEEKDSQETDGLLPGSRPST
                     RTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDP
                     DSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVC
                     VEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLV
                     GSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKA
                     NETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKD
                     LVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDV
                     AIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFR
                     ARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDR
                     KNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDL
                     ASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEE
                     GLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSC
                     MRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSN
                     NKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYL
                     WLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSL
                     FAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPP
                     IIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDL
                     LAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLK
                     RVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNI
                     RVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTVRLLRGYHMLCNGGPSSC
                     PTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEE
                     EPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRA
                     AKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGK
                     TGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVL
                     VRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASF
                     PMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDP
                     MKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSA
                     LRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDIS
                     PRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSL
                     TGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSAD
                     DFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNEND
                     EFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYF
                     LWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA"
     exon            189..247
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            248..336
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            337..436
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            437..618
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            619..692
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            693..819
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            820..874
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1006
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1069
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1154
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1314
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1501
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1688
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1912
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2021
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2202
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2323
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2324..2424
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2515
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2632
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2785
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2975
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2976..3100
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3101..3235
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3236..3397
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3517
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3518..3679
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3680..3842
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3843..3973
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3974..4003
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4004..4135
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4136..4202
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4339
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4340..4474
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4475..4612
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4613..4740
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4741..4911
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4912..5072
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5073..5215
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5216..5304
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5305..5403
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5404..5645
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5646..5824
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5825..5925
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5926..6128
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            6129..7118
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcaggaggag gaggcggcag cagccagaga agagggaggc gtgtgaacca cgctccgcca
       61 gcaagcttct tcccgctgct ctggcactac ccaggttctc tccgccagta tggccctgat
      121 cgtccacctc aagactgttt ctgagcttcg aggcagagct gatcggatcg ccaaagtcac
      181 tttccgagga cagtccttct attcccgggt cctggagaac tgcgaggatg tggctgactt
      241 cgatgagaca ttccggtggc cagtggctag cagcatcgac aggaatgaag tgttggagat
      301 tcagattttc aactacagca aagtcttcag caacaaacta atcgggacct tccgcatggt
      361 gctgcagaag gtggtggagg agaatcgggt agaggtgtct gacacgctga tcgatgacaa
      421 caatgctatc atcaagacca gcctaagcat ggaggtccgg tatcaagccg cagatggcac
      481 agtaggccct tgggatgatg gagactttct gggagatgaa tcccttcagg aggaagagaa
      541 agacagccag gagacagatg gtctgctacc tggctcccga cccagcaccc ggacacccgg
      601 cgagaagagc tttcgaagag cgggaaggag tgtattctcg gccatgaaac tcggcaagac
      661 tcggtcccac aaagaggagc cccaaagaca agatgagcca gcggtgctgg agatggagga
      721 cctggaccac ctagccatcc ggctggggga tgggctggat cctgactcag tgtctctagc
      781 ctcggtcact gctctcacca gcaatgtctc caacaaacgg tctaagccag atattaaaat
      841 ggaaccgagt gctgggagac ccatggatta ccaggtcagc atcacagtga ttgaggctcg
      901 gcagctggtg ggtttgaaca tggaccccgt ggtgtgcgtg gaggtgggcg atgacaaaaa
      961 atacacatcc atgaaggagt ccacaaactg cccttactac aacgagtact tcgtctttga
     1021 cttccacgtc tctcctgatg tcatgtttga caagatcatc aagatctcgg ttatccattc
     1081 taagaacctg cttcgaagcg gcactctggt gggctccttc aaaatggatg tggggaccgt
     1141 gtactcccag cctgaacacc agttccatca caaatgggct atcctgtcag accccgatga
     1201 catctctgcc gggctgaagg gctatgtaaa gtgtgatgtc gccgtggtgg gcaagggaga
     1261 caacatcaag acaccccaca aggccaacga gacagatgag gatgacattg aagggaactt
     1321 gctgctcccc gagggcgtgc ccccagaacg gcagtgggcc cggttctacg tgaaaattta
     1381 ccgagcagag ggactgcccc ggatgaacac aagcctcatg gccaacgtaa agaaggcgtt
     1441 catcggtgag aacaaggacc tcgtcgaccc ctacgttcaa gtcttctttg ctggacagaa
     1501 gggcaaaaca tcagtgcaga agagcagcta tgagccgctg tggaatgagc aggtcgtctt
     1561 tacagatctg ttccccccac tctgcaagcg catgaaggtg cagatccgag actccgacaa
     1621 ggtcaacgac gtggccatcg gcacccactt catcgacctg cgcaagattt ccaacgatgg
     1681 agacaaaggc ttcctgccta cgctcggccc agcctgggtg aacatgtacg gctccacacg
     1741 caactacacg ctgctggacg agcaccagga cctgaatgag ggcctgggtg agggggtgtc
     1801 cttccgtgcc cgcctcatgt tgggactggc tgtggagatc ctagacacct ccaacccaga
     1861 gctcaccagc tccaccgagg tgcaggtgga gcaggccaca cctgtctcag agagctgcac
     1921 agggagaatg gaagaatttt tcctatttgg agccttcctg gaagcctcaa tgatcgaccg
     1981 gaaaaatggg gacaagccag tgacctttga ggtgaccata ggaaactatg gcaatgaagt
     2041 cgatggtaca tcccggcccc agaggcctcg gccccggaag gaacctgggg atgaagaaga
     2101 ggtagacctg attcagaact ccagtgacga cgagggtgat gaagccgggg acctggcctc
     2161 agtgtcctcc accccaccta tgaggcccca gatcacagac aggaactatt tccacctgcc
     2221 ctacctagag cgcaagccct gcatctacat caagagctgg tggcctgacc agcggcgccg
     2281 tctctacaac gcaaacatca tggaccacat tgctgacaag ctggaagaag gtctgaatga
     2341 tgtccaggag atgatcaaaa cggagaagtc ctacccggag cgacgcctgc ggggtgtgct
     2401 agaggaactc agctgtggct gccaccgctt cctctccctc tcggacaagg accagggtca
     2461 ctcgtcccgc accaggctgg atcgggagcg cctcaaatcc tgtatgaggg agctggagag
     2521 catgggacaa caagccaaga gcctgagggc tcaggtgaag cgacacactg tacgggacaa
     2581 gctgaggtta tgccagaact tcctgcagaa gctacgcttc ctagctgatg agccccagca
     2641 cagcatcccc gatgtgttca tttggatgat gagtaataac aaacgtatcg cctacgcccg
     2701 cgtgccttcc aaagaccttt tgttctccat tgtggaggag gaattgggca aggactgcgc
     2761 caaggtcaag accctcttcc tgaagctgcc aggaaagagg ggcttcggct cggcaggctg
     2821 gacagtacag gccaagctgg aactctacct gtggctgggc ctcagcaagc agagaaagga
     2881 cttcctgtgt ggcctgcctt gtggcttcga ggaggtcaag gcagcccaag gcctgggtct
     2941 gcattccttt ccgcccatca gccttgtcta caccaagaag caaaccttcc agctccgagc
     3001 ccacatgtat caggcccgaa gcctcttcgc cgctgacagc actgggctct ctgatccttt
     3061 tgcccgagtc ttcttcatca accagagcca gtgcacggag gtcctaaacg agacactgtg
     3121 tcctacttgg gaccaaatgc tggtatttga caacctggag ctgtacggtg aagctcatga
     3181 gttacgagat gatcccccca tcattgtcat tgaaatctac gaccaggaca gcatgggcaa
     3241 agctgacttc atgggccgga cctttgccaa gcccctggtg aagatggcag acgaggcata
     3301 ttgcccgcct cgcttcccgc cccagcttga gtactaccag atctaccgag gcaacgccac
     3361 ggccggagac ctactggctg cctttgagct gctgcagatt ggaccatcgg ggaaggctga
     3421 cctgccaccc atcaatggcc cagtggacat ggacagaggg cccatcatgc ctgtgcctgt
     3481 gggaatccgg cccgtgctca gcaagtacag agtggaggtg ctgttctggg gcctgaggga
     3541 cttaaagagg gtgaacctgg cccaggtgga ccggccacgg gtggacatcg agtgtgcggg
     3601 aaaaggggta caatcgtccc tgatccacaa ttataagaag aaccccaact tcaacaccct
     3661 ggtcaagtgg tttgaagtgg acctcccaga gaatgagctc ctgcacccac ccttgaacat
     3721 ccgggtggtg gactgccggg cctttggacg atacaccctg gtgggctccc atgcagtcag
     3781 ctcactacga cgcttcatct accgaccccc ggaccgctcg gccgccaact ggaacaccac
     3841 agtcaggctt ctccggggct accacatgct gtgcaatggg ggcccctctt cttgccccac
     3901 aggggaggtt gtagtgagca tggaacctga agtgccagtt aagaagctgg agaccctggt
     3961 gaagctggat gcgacttctg atgctgtggt caaggtggat gtggctgaag atgagaagga
     4021 aaggaagaag aagaaaaaga aaggcccatc agaggaggca gaggaggagg aacccgatga
     4081 gagcatgctg gattggtggt ccaagtactt tgcctccatt gacacaatga aggagcaact
     4141 tcgacaacat gagacctctg ggattgattt ggaagagaag gaagaaatgg atagcactga
     4201 gggcctgaag gggccagtga agaacaagga gaagtccaga gctgcaaagg aggaaaaaaa
     4261 gaagaaaaat cagaaccctg gccctggcca gggatcggag gctcctgaga agaagaaagc
     4321 caagatcgac gagcttaagg tgtatcccaa ggagctagag tcggagtttg acaactttga
     4381 ggactggctg cacacgttca acctgttacg gggcaagact ggggatgatg aggatggctc
     4441 cacagaggaa gaacgcatag taggccgatt caagggttcc ctctgtgtgt acaaagtgcc
     4501 actcccggag gatgtgtctc gtgaagctgg ctacgatccc acctacggaa tgttccaggg
     4561 catcccaagc aatgacccca ttaatgtgct ggtccgaatc tatgtggtcc gggccacaga
     4621 cctgcacccg gctgacatca atggcaaagc cgacccctat atcgccatca agttaggcaa
     4681 gactgacatc cgagacaagg agaactacat ctccaagcag ctcaaccctg tctttggaaa
     4741 gtcctttgac attgaggcct ccttccccat ggagtccatg ctgacagtgg ctgtgtacga
     4801 ctgggatctg gtgggcactg atgacctcat cggagaaacc aagatcgacc tggaaaaccg
     4861 cttctacagc aagcaccgtg ctacctgcgg catcgcgcag acctattcca tacatggcta
     4921 taatatctgg agagacccca tgaagcccag ccagatcctg acccgcctct gcaaagaggg
     4981 caaagtggac ggcccccact ttggtcccca tgggagagtg aaggtcgcca accgtgtctt
     5041 cacagggcct tcagagatag aggatgagaa tggtcagagg aagcccacag atgagcacgt
     5101 ggcactgtct gctctgagac actgggagga catcccccgg gtgggctgcc gccttgtacc
     5161 ggagcacgtg gagaccaggc cgttgctcaa ccctgacaag ccaggcattg agcagggccg
     5221 cctggagctg tgggtggata tgttccccat ggatatgccg gcccctggaa cacctctgga
     5281 tatctccccc aggaaaccca agaagtatga gctgcgcgtc atcgtgtgga acacagatga
     5341 ggtggtcctg gaagacgacg atttcttcac gggagagaag tccagtgata tttttgtgag
     5401 ggggtggctg aagggccagc aggaggacaa acaggacaca gacgtacatt atcactccct
     5461 cacgggtgag ggcaacttca actggcgata cctgttcccc ttcgactacc tggcggccga
     5521 agagaagatc gttatgtcca agaaggagtc catgttctcc tgggatgaga cggagtacaa
     5581 gatacctgcg cggctcaccc tgcagatctg ggacgctgac cacttctcag ccgatgactt
     5641 cctgggggcc atcgagctgg acctgaaccg gttcccgagg ggcgccaaga cagccaagca
     5701 gtgcaccatg gagatggcca ccggggaggt ggatgtgccc ctggtttcca tctttaaaca
     5761 gaaacgtgtc aaaggctggt ggcccctcct ggcccgcaat gagaatgatg aatttgaact
     5821 cacgggcaaa gtagaggcag agttacacct cctcacggca gaggaggcgg agaagaaccc
     5881 ggtgggcctg gctcgcaatg aacctgaccc tctagaaaaa cccaatcggc ccgacacaag
     5941 cttcatctgg ttcttgaacc ctctcaagtc tgcccgctac ttcctgtggc atacctaccg
     6001 ctggctgctc ctcaaactcc tgctgctcct cctcctgctg ctgctgctcg ccctgtttct
     6061 ctactctctg cctggctacc tggccaagaa gatccttggg gcctgagccc tgcagccgcc
     6121 tgggtctgcc gacccgacac ggcgttcgtc tggttcctga acccactcaa atctatcaag
     6181 tacctcattt gcacccggta caagtggctt atcatcaaga ttgtgctggc cctgcttggg
     6241 ctgctcatgc tggccctctt cctctatagc ctcccaggct acatggtcaa gaagctccta
     6301 ggggcatgaa gcgtgcccac cctagcccgc cccagcccgc cccagcatcc ctccaggggc
     6361 tgctgcgttt gccttccctc acctggactc tctcccagct ccctgcggaa ccgaccacgc
     6421 ctggcagcct tgagcaagac gcttgctggg cttccatccc caccccatcc ccaaagtgct
     6481 gcttgcctga actagtccca tgcctgcctg gggtttgggg tacagctgac cttcagaacc
     6541 agatactctc tcgccccact ttctaaggta ccaagttcct ctgcccagcc ccaagagtgg
     6601 tagtggccca accctccctt gtgctctcca aatcgtgtgt taaggctcca gtgaaatgaa
     6661 ccaaggaaca tggggcagtg tccaaggctc cgataagtag gaacacgtgt aaagcaccag
     6721 aaatgccagc agaggcagca catcactcta caaagcatcc tgaccaagat ggcagcggtg
     6781 gccagctgct cctgcgcatg ctctcctctt ggctccccct ccccccacag ccacttggcc
     6841 acgtagcatc aggctcctat caccagggat gcctctgcct cggccagccc agcgcgtgcc
     6901 agctagtatg caagctccaa gagtaaatcg ggtgtcctta gccgccctga atactgccct
     6961 gtcctgcctg ccgacggcca cacccgagtg tacgctgagt gctgccctcc tgcctctgcg
     7021 ggtctgctgg ctgcttttct tgagtgtgat tttttctctc tctctctccc tctctctcgc
     7081 tcaataaaat aaactctgag acagaagtgg agcagaaa
//
