LOCUS       NM_053904               4003 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), mRNA.
ACCESSION   NM_053904
VERSION     NM_053904.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ye,G.J., Breslow,E.B. and Meister,A.
  TITLE     The amino acid sequence of rat kidney 5-oxo-L-prolinase determined
            by cDNA cloning
  JOURNAL   J Biol Chem 271 (50), 32293-32300 (1996)
   PUBMED   8943290
  REMARK    Erratum:[J Biol Chem 1997 Feb 14;272(7):4646. Guo-jie GE [corrected
            to Ye GJ]]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from U70825.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U70825.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..4003
                     /gene="Oplah"
                     /note="5-oxoprolinase (ATP-hydrolysing)"
                     /db_xref="GeneID:116684"
                     /db_xref="RGD:620956"
     exon            1..52
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            53..276
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..3972
                     /gene="Oplah"
                     /EC_number="3.5.2.9"
                     /note="5-OPase; pyroglutamase; 5-oxo-L-prolinase"
                     /codon_start=1
                     /product="5-oxoprolinase"
                     /protein_id="NP_446356.1"
                     /db_xref="GeneID:116684"
                     /db_xref="RGD:620956"
                     /translation="MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYP
                     DAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTR
                     GFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDL
                     LEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSL
                     SSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAP
                     MDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFE
                     ASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVT
                     DANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSL
                     EEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTV
                     HIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQG
                     FPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFII
                     PERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGEL
                     GYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSI
                     FSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQE
                     TVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHH
                     ADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTR
                     NLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFG
                     TSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAI
                     TLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVD
                     VILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRIT
                     DPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPY
                     GLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPP
                     PGSPPLFPAFPERGSVFEYRRAQEAV"
     exon            277..468
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            469..568
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            569..692
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            693..888
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1054
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1193
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1261
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1527
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1640
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1811
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1949
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2088
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2201
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2353
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2514
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2515..2616
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2791
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..2976
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3123
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3258
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3408
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            3409..3566
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            3567..3727
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            3728..3825
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     exon            3826..4003
                     /gene="Oplah"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4003
                     /gene="Oplah"
                     /note="13 A nucleotides"
ORIGIN      
        1 agcacattca ccctctgagg caagtcgccg gccttttcag cgttttttgc tgttctccca
       61 gggttaccag ctgaagagcc attcctggac tccagcttca acgtcatggg cagcccagaa
      121 gggcgcttcc atttcgccat cgaccgcggt ggcaccttca cagatgtctt tgcccagtgc
      181 cctggagggc atgtgcgtgt cctgaagctg ctctcagagg accctgccaa ctatccagat
      241 gcacccacag agggcatccg ccgaattcta gagcaggagg agggtgtgct gctgcctcga
      301 ggccgaccgc tagacaccag tcgcattgcc agcatccgca tgggtaccac ggtggccacc
      361 aatgcactgt tggaacgaca gggagaacgg gtggcactgc tggtgactcg gggtttccga
      421 gacctgctgc atattggcac tcaggcccgc ccggacctct ttgacttggc tgtgcccatg
      481 ccagaggttc tgtatgagga agtgctggag gtagatgagc gagtggtgct gtatcgcgga
      541 gaaccaggtg ccggctctcc tgtcaaaggc cgcacagggg acctgctaga gatacagcag
      601 cctgtggacc tggaagccct gcgtgggaag ctggaggggc tcttgtctcg gggcattcac
      661 agtctggcag tggtgctcat gcattcgtac acgtgggccc agcatgagca gcaggtgggc
      721 acgctggccc gggagctggg cttcacgcac gtctccttgt cctcggaagt catgcccatg
      781 gtacgaattg ttcctcgggg ccatacagcc tgtgctgacg cttaccttac tcccaccatc
      841 cagcgctatg tgcagggctt ccgccgaggc ttccagggcc agctaaagaa tgtgcaagtt
      901 ctcttcatgc gctctgatgg tggcctcgca cccatggatg ctttcagtgg ttcccgggct
      961 gtgctctctg gccctgctgg gggtgtggtt ggctactcag ctaccaccta ccatctggaa
     1021 ggcggtcagc ctgtcattgg ctttgacatg ggaggcacat ccacagacgt gagccgctat
     1081 gctggagaat ttgagcatgt ctttgaggct agcacagcag gcgttaccct tcaggcaccc
     1141 cagttggaca tcaacacagt ggcagctggc gggggttccc gcctcttctt cagatctggc
     1201 ctctttgtgg ttggtccaga gtcagcaggt gcccacccag gtcctgcctg ctaccgtaaa
     1261 gggggtcctg tgacagtgac agatgctaat ctggtcctgg gtcgcctgct gcctgcctcc
     1321 ttcccctgca tttttgggcc aggagaagac cagccactgt ctcctgaggc ttcccgaaag
     1381 gctctagagg ctgtggccat ggaggtcaac agtttcttga ccaatggacc gtgcccagct
     1441 tcccaactaa gtctggaaga ggtggccatg gggtttgtgc gtgttgccaa tgaagccatg
     1501 tgccggccta tccgtgccct cacacaggca cgaggccatg acccctcagc ccatgtattg
     1561 gcttgctttg gaggagctgg tgggcaacac gcttgtgcca ttgcccgggc cctggggatg
     1621 gatactgtgc acattcacag gcacagcggg ctgctgtcag cactaggact ggccttggca
     1681 gatgtggttc acgaagcaca ggagccctgt tccctgtctt acacacctga aacctttgca
     1741 caactggacc agagactgag ccgcctggag gagcagtgtg tggatgcctt gcaggtccag
     1801 ggcttcccta ggtctcagat cagcaccgag agcttcctgc atcttcgcta ccaaggcact
     1861 gactgcgccc taatggtgtc tgcccatcag catccggcca cagcctgctc accccgagct
     1921 ggtgactttg gagccgcatt tgtggagagg tacatgagag agtttggctt cattatcccc
     1981 gagcggccgg tggtggtaga tgatgtacgt gtgaggggaa ctggccgtag tggacttcag
     2041 ctggaggaca cccccaaaat ccagactgga cctccccacg tggaaaaggt gacccagtgc
     2101 tactttgaag ggggttatca ggagactccc gtgtaccttt taggagaact aggctacggg
     2161 caccagctcc aagggccctg ccttatcatc gacaacaaca gcaccatcct tgtagaaccg
     2221 ggttgccaag cagaggtgac tgatacaggg gacatccgca tttctgtggg agctgagggt
     2281 cctagtatgg cagataccag gcttgacccc atccagctgt ctattttctc acaccgcttc
     2341 atgagcattg ctgagcagat gggccgcatc ctacagcgca cagccatctc taccaacatc
     2401 aaggaacgcc tcgacttctc ctgtgccctc tttgggccag atgggggcct cgtctccaat
     2461 gctccccaca ttcctgtgca cctgggtgcc atgcaagaga ctgtacagtt ccagattcag
     2521 cacttaggag ccgacctcca tcctggtgat gtgttgctca gcaaccatcc cagcgcaggg
     2581 ggcagccatc ttcctgacct gactgtcatt acaccggtgt tttggccagg ccagacgagg
     2641 cctgtgttct acgtggctag ccgagggcac cacgcagaca ttggaggaat cacaccgggc
     2701 tctatgccgc ctcactccac cacgctgcaa caggagggtg ccgtttttct gtccttcaaa
     2761 ctggtccagg gaggcgtctt ccaggaagag gcagtgacag aggccctacg ggcaccaggc
     2821 aagatctctg gctgtagtgg aaccaggaac ctgcatgaca acctgtcgga tcttcgtgcc
     2881 caggtggcag ctaaccagaa aggcatccag ctggtgggag agctgatcgg acagtatggc
     2941 ttagatgtgg tgcaggccta tatgggccat attcaggcga atgctgagct agcagtgaga
     3001 gacatgctcc gggcttttgg aacttcccgg caggccaggg gcctgcccct ggaggtgtct
     3061 gcagaggatc acatggatga tggctctccc atctgtctgc gtgttcagat caacctgagt
     3121 cagggcagtg cggtatttga ctttactggt tccgggtctg aggtgtttgg caatctcaat
     3181 gccccgagag ccataacact gtctgctctc atctattgct tacgctgtct agtgggccgt
     3241 gacatcccac ttaaccaggg ttgcctggct cctgtgcgtg tcataattcc caaaggctcc
     3301 atattggatc catccccaga ggcagcagtg gtcggcggca acgtgctcac atctcagcga
     3361 gtagtggatg tcattctggg ggcttttggg gcctgttcag cctcccaggg ctgcatgaac
     3421 aatgtgaccc tgggcaatgc ccgtatgggc tactatgaga cagtggctgg tggtgccggt
     3481 gcgggccctg gctggcatgg gcgcagtggt gtacacagtc acatgaccaa cacacgcatt
     3541 acggatccag agattctgga gagtcggtat ccagttatcc tgcgccgctt tgagctgagg
     3601 ccaggctccg ggggccgagg tcgcttccgg ggaggtgatg gcgtagtccg agagctggtc
     3661 tttcgggaag aggcgctgtt gtctgtgctc accgagcgcc gggccttcca gccttacggc
     3721 ctccacgggg gagagcctgg tgcgcgtggc ttaaacctcc tgatcagaaa agatgggcgc
     3781 acagtgaatt tgggcggcaa gacatctgtg accgtgtacc ccggggacgt gttctgcctc
     3841 cacacgcctg ggggtggggg ctacggagac ccggaggatc cagcgccacc accaggctcg
     3901 cccccgctat ttccagcctt ccccgagcgc ggcagtgtat tcgagtaccg ccgcgcccag
     3961 gaagccgtat gagtgcccca caataaagat cctttgaatc gca
//
