LOCUS       NM_001101018             878 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Rattus norvegicus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.
ACCESSION   NM_001101018
VERSION     NM_001101018.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Ramos-Molina,B., Lambertos,A., Lopez-Contreras,A.J., Kasprzak,J.M.,
            Czerwoniec,A., Bujnicki,J.M., Cremades,A. and Penafiel,R.
  TITLE     Structural and degradative aspects of ornithine decarboxylase
            antizyme inhibitor 2
  JOURNAL   FEBS Open Bio 4, 510-521 (2014)
   PUBMED   24967154
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  AUTHORS   Bastos-Amador,P., Royo,F., Gonzalez,E., Conde-Vancells,J.,
            Palomo-Diez,L., Borras,F.E. and Falcon-Perez,J.M.
  TITLE     Proteomic analysis of microvesicles from plasma of healthy donors
            reveals high individual variability
  JOURNAL   J Proteomics 75 (12), 3574-3584 (2012)
   PUBMED   22516433
REFERENCE   3  (bases 1 to 878)
  AUTHORS   Olsen,R.R. and Zetter,B.R.
  TITLE     Evidence of a role for antizyme and antizyme inhibitor as
            regulators of human cancer
  JOURNAL   Mol Cancer Res 9 (10), 1285-1293 (2011)
   PUBMED   21849468
  REMARK    Review article
REFERENCE   4  (bases 1 to 878)
  AUTHORS   Ruan,Y., Cheng,M., Ou,Y., Oko,R. and van der Hoorn,F.A.
  TITLE     Ornithine decarboxylase antizyme Oaz3 modulates protein phosphatase
            activity
  JOURNAL   J Biol Chem 286 (33), 29417-29427 (2011)
   PUBMED   21712390
  REMARK    GeneRIF: Ornithine decarboxylase antizyme Oaz3 modulates protein
            phosphatase activity.
REFERENCE   5  (bases 1 to 878)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   6  (bases 1 to 878)
  AUTHORS   Kanerva,K., Makitie,L.T., Pelander,A., Heiskala,M. and
            Andersson,L.C.
  TITLE     Human ornithine decarboxylase paralogue (ODCp) is an antizyme
            inhibitor but not an arginine decarboxylase
  JOURNAL   Biochem J 409 (1), 187-192 (2008)
   PUBMED   17900240
REFERENCE   7  (bases 1 to 878)
  AUTHORS   Fitzgerald,C., Sikora,C., Lawson,V., Dong,K., Cheng,M., Oko,R. and
            van der Hoorn,F.A.
  TITLE     Mammalian transcription in support of hybrid mRNA and protein
            synthesis in testis and lung
  JOURNAL   J Biol Chem 281 (50), 38172-38180 (2006)
   PUBMED   17040916
  REMARK    GeneRIF: N-terminal protein sequencing suggests translation
            initiation from a non-AUG (CUG) start site.
REFERENCE   8  (bases 1 to 878)
  AUTHORS   Lopez-Contreras,A.J., Lopez-Garcia,C., Jimenez-Cervantes,C.,
            Cremades,A. and Penafiel,R.
  TITLE     Mouse ornithine decarboxylase-like gene encodes an antizyme
            inhibitor devoid of ornithine and arginine decarboxylating activity
  JOURNAL   J Biol Chem 281 (41), 30896-30906 (2006)
   PUBMED   16916800
REFERENCE   9  (bases 1 to 878)
  AUTHORS   Zhang,J., Wang,Y., Zhou,Y., Cao,Z., Huang,P. and Lu,B.
  TITLE     Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are
            potential interaction partners of testicular germ cell-specific
            protein GGN1
  JOURNAL   FEBS Lett 579 (2), 559-566 (2005)
   PUBMED   15642376
REFERENCE   10 (bases 1 to 878)
  AUTHORS   Ivanov,I.P., Rohrwasser,A., Terreros,D.A., Gesteland,R.F. and
            Atkins,J.F.
  TITLE     Discovery of a spermatogenesis stage-specific ornithine
            decarboxylase antizyme: antizyme 3
  JOURNAL   Proc Natl Acad Sci U S A 97 (9), 4808-4813 (2000)
   PUBMED   10781085
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DQ431008.1 and AW529628.1.
            
            Summary: The protein encoded by this gene belongs to the ornithine
            decarboxylase antizyme family, which plays a role in cell growth
            and proliferation by regulating intracellular polyamine levels.
            Expression of antizymes requires +1 ribosomal frameshifting, which
            is enhanced by high levels of polyamines. Antizymes in turn bind to
            and inhibit ornithine decarboxylase (ODC), the key enzyme in
            polyamine biosynthesis; thus, completing the auto-regulatory
            circuit. This gene encodes antizyme 3, the third member of the
            antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC
            activity and polyamine uptake; however, it does not stimulate ODC
            degradation. Also, while antizymes 1 and 2 have broad tissue
            distribution, expression of antizyme 3 is restricted to haploid
            germ cells in testis, suggesting a distinct role for this antizyme
            in spermiogenesis. Antizyme 3 gene knockout studies showed that
            homozygous mutant male mice were infertile, and indicated the
            likely role of this antizyme in the formation of a rigid connection
            between the sperm head and tail during spermatogenesis. This
            transcript initiates translation from a non-AUG (CUG) codon that is
            highly conserved among the antizyme 3 orthologs. [provided by
            RefSeq, Dec 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ431008.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 17040916
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ribosomal slippage       :: inferred from conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-860               DQ431008.1         1-860
            861-878             AW529628.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..878
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /note="ornithine decarboxylase antizyme 3"
                     /db_xref="GeneID:689588"
                     /db_xref="RGD:1599278"
     exon            1..178
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /inference="alignment:Splign:2.1.0"
     CDS             join(12..269,271..744)
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /ribosomal_slippage
                     /note="protein translation is dependent on
                     polyamine-induced +1 ribosomal frameshift; non-AUG (CUG)
                     translation initiation codon; antizyme 3"
                     /codon_start=1
                     /product="ornithine decarboxylase antizyme 3"
                     /protein_id="NP_001094488.1"
                     /db_xref="GeneID:689588"
                     /db_xref="RGD:1599278"
                     /translation="MPCTRSRPSLYSLSYIKRGKTRNCLYPFWSPYAYYLYCYKYRIT
                     LREKMLPCCYRSITYKEQEDLTLRPHCCLPCSCLPYSCLPCSESLEGLQVGRSTAQEK
                     DHSQLKELYSAGNLTVLSADPLLHQDPVQLDFHFRLTPHSSAHWHGLLCDHQLFLDIP
                     FQALEQGNRESLTATLEYVEEKTNVDSVFVNFQSNHKDRGALLRAFSYMGFEVVRPDH
                     PALPPWDNVIFMVYPLERDLGQPGQ"
     exon            179..348
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /inference="alignment:Splign:2.1.0"
     exon            349..524
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /inference="alignment:Splign:2.1.0"
     exon            525..610
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /inference="alignment:Splign:2.1.0"
     exon            611..864
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      843..848
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
     polyA_site      864
                     /gene="Oaz3"
                     /gene_synonym="Az3; Oaz-t; ODC-Az 3"
ORIGIN      
        1 gaacagagaa actgccttgt accaggtccc gcccctctct ctactccctt tcttatatta
       61 agaggggaaa aacacggaac tgcctctacc cattctggtc accatacgcc tattacctct
      121 actgttacaa ataccggatc accctccggg agaagatgct gccttgttgt tacagaagca
      181 tcacttacaa ggaacaggag gacctgactc tccggcccca ttgctgcctc ccgtgctcct
      241 gcctcccgta ctcctgcctc ccgtgctcct gagtccctag aaggactcca ggtgggtagg
      301 agcactgcac aggaaaaaga ccacagccag cttaaagaac tctattcagc tgggaacctg
      361 acagtgctat cagccgaccc cctgcttcac caagacccag ttcagttaga cttccacttt
      421 cgtcttaccc cccattcctc tgctcattgg cacggccttc tgtgtgacca ccaactcttc
      481 ctggatatcc catttcaggc cttggagcaa ggcaaccgag aaagtttgac agcaacactg
      541 gagtatgtgg aggagaaaac caatgtggac tctgtgtttg tgaacttcca aagcaatcat
      601 aaggacagag gtgccctgct tcgagccttt agctacatgg gctttgaggt ggtcagacca
      661 gatcatccag ccctccctcc ctgggacaat gtcatcttca tggtgtatcc ccttgaaagg
      721 gaccttggcc agcctggcca gtgagcctcc ctaaacatgt tctctctctg tgaggggttg
      781 aaaacctcaa cacacgggac tctggggccc aggatgtgat ttaagacact tccatcctag
      841 gaaataaagg gtagtgcaat caaaaaaaaa aaaaaaaa
//
