LOCUS       NM_139081               1067 bp    mRNA    linear   ROD 04-APR-2024
DEFINITION  Rattus norvegicus ornithine decarboxylase antizyme 1 (Oaz1),
            transcript variant 1, mRNA.
ACCESSION   NM_139081
VERSION     NM_139081.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ramos-Molina,B., Lambertos,A., Lopez-Contreras,A.J., Kasprzak,J.M.,
            Czerwoniec,A., Bujnicki,J.M., Cremades,A. and Penafiel,R.
  TITLE     Structural and degradative aspects of ornithine decarboxylase
            antizyme inhibitor 2
  JOURNAL   FEBS Open Bio 4, 510-521 (2014)
   PUBMED   24967154
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Olsen,R.R. and Zetter,B.R.
  TITLE     Evidence of a role for antizyme and antizyme inhibitor as
            regulators of human cancer
  JOURNAL   Mol Cancer Res 9 (10), 1285-1293 (2011)
   PUBMED   21849468
  REMARK    Review article
REFERENCE   3  (bases 1 to 1067)
  AUTHORS   Ma,J.M., Yang,H., Dong,J.F., Ning,Q.J. and Li,J.K.
  TITLE     The influence of corticosterone on antizyme gene expression in
            early regenerating rat liver
  JOURNAL   Acta Gastroenterol Belg 74 (2), 289-294 (2011)
   PUBMED   21861313
  REMARK    GeneRIF: Corticosterone treatment induces antizyme 1 protein
            synthesis in early regenerating rat liver. However, it has little
            effect on antizyme 1 gene transcription.
REFERENCE   4  (bases 1 to 1067)
  AUTHORS   Gubern,C., Hurtado,O., Rodriguez,R., Morales,J.R., Romera,V.G.,
            Moro,M.A., Lizasoain,I., Serena,J. and Mallolas,J.
  TITLE     Validation of housekeeping genes for quantitative real-time PCR in
            in-vivo and in-vitro models of cerebral ischaemia
  JOURNAL   BMC Mol Biol 10, 57 (2009)
   PUBMED   19531214
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1067)
  AUTHORS   Gandre,S., Bercovich,Z. and Kahana,C.
  TITLE     Mitochondrial localization of antizyme is determined by
            context-dependent alternative utilization of two AUG initiation
            codons
  JOURNAL   Mitochondrion 2 (4), 245-256 (2003)
   PUBMED   16120325
REFERENCE   6  (bases 1 to 1067)
  AUTHORS   Kankare,K., Uusi-Oukari,M. and Janne,O.A.
  TITLE     Structure, organization and expression of the mouse ornithine
            decarboxylase antizyme gene
  JOURNAL   Biochem J 324 (Pt 3) (Pt 3), 807-813 (1997)
   PUBMED   9210404
REFERENCE   7  (bases 1 to 1067)
  AUTHORS   Pujic,Z., Matsumoto,I., Yamataka,A., Miyano,T. and Wilce,P.
  TITLE     Induction of immediate-early, ornithine decarboxylase and antizyme
            gene expression in the rat small intestine after transient
            ischaemia
  JOURNAL   Life Sci 58 (25), 2289-2296 (1996)
   PUBMED   8649218
REFERENCE   8  (bases 1 to 1067)
  AUTHORS   Matsufuji,S., Matsufuji,T., Miyazaki,Y., Murakami,Y., Atkins,J.F.,
            Gesteland,R.F. and Hayashi,S.
  TITLE     Autoregulatory frameshifting in decoding mammalian ornithine
            decarboxylase antizyme
  JOURNAL   Cell 80 (1), 51-60 (1995)
   PUBMED   7813017
  REMARK    GeneRIF: antizyme expression requires programmed,+1 ribosomal
            frameshifting
REFERENCE   9  (bases 1 to 1067)
  AUTHORS   Mitchell,J.L., Judd,G.G., Bareyal-Leyser,A. and Ling,S.Y.
  TITLE     Feedback repression of polyamine transport is mediated by antizyme
            in mammalian tissue-culture cells
  JOURNAL   Biochem J 299 (Pt 1) (Pt 1), 19-22 (1994)
   PUBMED   8166639
REFERENCE   10 (bases 1 to 1067)
  AUTHORS   Miyazaki,Y., Matsufuji,S. and Hayashi,S.
  TITLE     Cloning and characterization of a rat gene encoding ornithine
            decarboxylase antizyme
  JOURNAL   Gene 113 (2), 191-197 (1992)
   PUBMED   1572540
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from FQ216817.1.
            
            On Jul 3, 2014 this sequence version replaced NM_139081.1.
            
            Summary: The protein encoded by this gene belongs to the ornithine
            decarboxylase antizyme family, which plays a role in cell growth
            and proliferation by regulating intracellular polyamine levels.
            Expression of antizymes requires +1 ribosomal frameshifting, which
            is enhanced by high levels of polyamines. Antizymes in turn bind to
            and inhibit ornithine decarboxylase (ODC), the key enzyme in
            polyamine biosynthesis; thus, completing the auto-regulatory
            circuit. This gene encodes antizyme 1, the first member of the
            antizyme family, that has broad tissue distribution, and negatively
            regulates intracellular polyamine levels by binding to and
            targeting ODC for degradation, as well as inhibiting polyamine
            uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and
            studies in rat suggest that alternative use of the two translation
            initiation sites results in N-terminally distinct protein isoforms
            with different subcellular localization. Alternatively spliced
            transcript variants have also been noted for this gene. [provided
            by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) encodes two isoforms resulting
            from the use of alternative in-frame translation initiation codons.
            The longer isoform (1, also known as 29 kDa form or AZ-1) is
            derived from an upstream AUG (at nt 80-82), whereas the shorter
            isoform (2, also known as 24.5 kDa form or AZ-2) is derived from a
            downstream AUG (at nt 179-181). This RefSeq represents the longer
            isoform, which is localized to the mitochondria (PMID:16120325).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ211375.1, FQ213504.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 16120325
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ribosomal slippage                 :: PMID: 7813017
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1067              FQ216817.1         1-1067
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..1067
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /note="ornithine decarboxylase antizyme 1"
                     /db_xref="GeneID:25502"
                     /db_xref="RGD:3219"
     exon            1..225
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /inference="alignment:Splign:2.1.0"
     CDS             join(80..283,285..764)
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /ribosomal_slippage
                     /note="protein translation is dependent on +1
                     polyamine-induced ribosomal frameshift; isoform 1 is
                     encoded by transcript variant 1"
                     /codon_start=1
                     /product="ornithine decarboxylase antizyme 1 isoform 1"
                     /protein_id="NP_620781.1"
                     /db_xref="GeneID:25502"
                     /db_xref="RGD:3219"
                     /translation="MVKSSLQRILNSHCFAREKEGDKRSATLHASRTMPLLSQHSRGG
                     CSSESSRVALHCCSNLGPGPRWCSDVPHPPLKIPGGRGNSQRDHSLSASILYSDERLN
                     VTEEPTSNDKTRVLSIQCTLTEAKQVTWRAVWNGGGLYIELPAGPLPEGSKDSFAALL
                     EFAEEQLRADHVFICFPKNREDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMVYTL
                     EREDPGEED"
     exon            226..371
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      290..342
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /note="recoding_pseudoknot"
                     /function="stimulates +1 ribosomal frameshifting"
     exon            372..544
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            545..630
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            631..1049
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      830..835
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
     polyA_site      872
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
     regulatory      912..917
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
     polyA_site      938
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
     regulatory      1027..1032
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
     polyA_site      1049
                     /gene="Oaz1"
                     /gene_synonym="Oaz; ODC-Az; ODCAC"
ORIGIN      
        1 attttgcgaa cggcgagcag cggcggcggc gcgggaagtg cagcggaggt ttttgctggt
       61 ttcggacccc agcggccgga tggtgaaatc ctccctgcag cggatcctca acagccactg
      121 cttcgccaga gagaaagaag gggacaaacg cagcgccacg cttcacgcca gccgcaccat
      181 gccgcttctt agtcagcaca gccgcggcgg ctgcagcagc gagagttcta gggttgccct
      241 tcattgctgt agtaacctgg gtccggggcc tcggtggtgc tcctgatgtc cctcacccac
      301 ccctgaagat cccaggtggg cgagggaaca gtcagcggga tcacagtctt tcagctagca
      361 tcctgtactc cgacgagcgg ctgaatgtga cagaggagcc gacgtctaac gacaagacca
      421 gggtcctgag catccagtgc acgctcacgg aggccaagca ggtcacctgg agggcggtgt
      481 ggaatggtgg tggcctctac atcgagctcc cagctgggcc tctgccagaa ggcagcaagg
      541 acagttttgc agctctacta gagttcgcag aggagcagct ccgggccgac cacgttttca
      601 tctgcttccc caagaaccgt gaggacagag ccgccctact ccgaaccttc agctttcttg
      661 gctttgagat tgtgagaccc gggcatcccc tcgtccccaa gagacccgac gcttgcttca
      721 tggtctacac gctggagaga gaggacccgg gtgaggagga ctaggtgcca gccctgccca
      781 gtgtccctgc accctctccc gggtttgtcc acatgtcatg attgtgcaga ataaacgctc
      841 actccattag cggggtgctt cttcgagctg aatgctgtgt ttgtcacact caagtggtgg
      901 ctttaattct aaataaaggt ttctatttta cttttttatt gctgtttaag atggtcaggt
      961 gacctgtttt agagcagcct cctttgaagt ctggaaaaat ggtgtcacct cccctggctc
     1021 aaacctaata aataaagtga tctcgttcaa aaaaaaaaaa aaaaaaa
//
