LOCUS       NM_017361               2277 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus nucleoporin 54 (Nup54), mRNA.
ACCESSION   NM_017361
VERSION     NM_017361.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Sharma,A., Solmaz,S.R., Blobel,G. and Melcak,I.
  TITLE     Ordered Regions of Channel Nucleoporins Nup62, Nup54, and Nup58
            Form Dynamic Complexes in Solution
  JOURNAL   J Biol Chem 290 (30), 18370-18378 (2015)
   PUBMED   26025361
  REMARK    GeneRIF: we carried out solution analysis utilizing the entire
            ordered regions of Nup62, Nup54, and Nup58, and demonstrate that
            they form a dynamic 'triple complex'
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Koh,J. and Blobel,G.
  TITLE     Allosteric Regulation in Gating the Central Channel of the Nuclear
            Pore Complex
  JOURNAL   Cell 161 (6), 1361-1373 (2015)
   PUBMED   26046439
REFERENCE   3  (bases 1 to 2277)
  AUTHORS   Bonnet,A. and Palancade,B.
  TITLE     Regulation of mRNA trafficking by nuclear pore complexes
  JOURNAL   Genes (Basel) 5 (3), 767-791 (2014)
   PUBMED   25184662
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2277)
  AUTHORS   Ulrich,A., Partridge,J.R. and Schwartz,T.U.
  TITLE     The stoichiometry of the nucleoporin 62 subcomplex of the nuclear
            pore in solution
  JOURNAL   Mol Biol Cell 25 (9), 1484-1492 (2014)
   PUBMED   24574455
  REMARK    GeneRIF: A 1:1:1 stoichiometry of the Nup62 complex, where Nup54 is
            central with direct binding to Nup62 and Nup58.
REFERENCE   5  (bases 1 to 2277)
  AUTHORS   Solmaz,S.R., Chauhan,R., Blobel,G. and Melcak,I.
  TITLE     Molecular architecture of the transport channel of the nuclear pore
            complex
  JOURNAL   Cell 147 (3), 590-602 (2011)
   PUBMED   22036567
  REMARK    GeneRIF: Study reports the crystal structures of the Nup54-Nup58
            and Nup62-Nup54 interacting domains and reconstruct the molecular
            architecture of the NPC transport channel.
REFERENCE   6  (bases 1 to 2277)
  AUTHORS   Ben-Efraim,I. and Gerace,L.
  TITLE     Gradient of increasing affinity of importin beta for nucleoporins
            along the pathway of nuclear import
  JOURNAL   J Cell Biol 152 (2), 411-417 (2001)
   PUBMED   11266456
REFERENCE   7  (bases 1 to 2277)
  AUTHORS   Hu,T., Guan,T. and Gerace,L.
  TITLE     Molecular and functional characterization of the p62 complex, an
            assembly of nuclear pore complex glycoproteins
  JOURNAL   J Cell Biol 134 (3), 589-601 (1996)
   PUBMED   8707840
REFERENCE   8  (bases 1 to 2277)
  AUTHORS   Guan,T., Muller,S., Klier,G., Pante,N., Blevitt,J.M., Haner,M.,
            Paschal,B., Aebi,U. and Gerace,L.
  TITLE     Structural analysis of the p62 complex, an assembly of O-linked
            glycoproteins that localizes near the central gated channel of the
            nuclear pore complex
  JOURNAL   Mol Biol Cell 6 (11), 1591-1603 (1995)
   PUBMED   8589458
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from U63840.1.
            
            [WARNING] On Oct 10, 2024 this sequence was replaced by
            NM_017361.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U63840.1, BC078858.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132268
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p22"
     gene            1..2277
                     /gene="Nup54"
                     /note="nucleoporin 54"
                     /db_xref="GeneID:53372"
                     /db_xref="RGD:619926"
     exon            1..133
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..1599
                     /gene="Nup54"
                     /note="54 kDa nucleoporin; nucleoporin Nup54; nucleoporin
                     p54"
                     /codon_start=1
                     /product="nuclear pore complex protein Nup54"
                     /protein_id="NP_059057.1"
                     /db_xref="GeneID:53372"
                     /db_xref="RGD:619926"
                     /translation="MAFNFGAPSGTSGTSTATAAPAGGFGGFGTTTTTAGSAFSFSAP
                     TNTGSTGLLGGTQNKGFGFGTGFGTSTGTGTGLGTGLGTGLGFGGFNTQQQQQQQQTS
                     LGGLFSQPAQAPAQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGYFNN
                     NIPPVEFTQENPFCRFKAVGYSCMPNNKDEDGLVVLIFNKKETDIRSQQQQLVESLHK
                     VLGGNQTLTVNVEGIKTLPDDQTEVVIYIVERSPNGTSRRVPATTLYAHFEQANIKTQ
                     LQQLGVTLSMTRTELSPAQIKQLLQNPPAGVDPIIWEQAKVDNPDSEKLIPVPMVGFK
                     ELLRRLKVQDQMTKQHQTRLDIISEDISELQKNQTTTMAKIAQYKRKLMDLSHRTLQV
                     LIKQEIQRKSGYAIQAEEEQLRVQLDTIQGELNAPTQFKGRLNELMSQIRMQNHFGAV
                     KSEEKYYIDADLLREIKQHLKQQQEGLSHLISIIKDDLEDIKLVEHGLNETIHSRGGV
                     FS"
     exon            134..217
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            218..370
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            371..597
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            598..785
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            786..982
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1037
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1131
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1239
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1359
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1470
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..2257
                     /gene="Nup54"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aagagcggag agcactgggc gtcgcgccac cggagccggg gaccacttaa gtgctgctcc
       61 cgcgacatgg cattcaattt tggggctcct tcgggcactt cgggcacctc tacagccacc
      121 gcggcaccgg cgggcgggtt tggcggattt ggaacaacaa ctacaactgc aggctctgca
      181 ttcagctttt ctgccccaac aaacacaggc agtacaggcc ttctcggcgg cactcagaac
      241 aaaggttttg gctttggcac tggttttggc acatcgacgg gtactggcac tggtttaggc
      301 actggcttgg gaaccggact tggattcgga ggatttaaca cccagcagca gcagcagcag
      361 cagcagactt ctttaggcgg tctcttcagt cagcctgcac aggcccctgc gcagtccaac
      421 caactcatca acactgccag cgctctttct gctccaacgc tattggggga tgagagagat
      481 gccatcttgg caaagtggaa ccagttgcag gccttctggg gaacaggaaa aggatatttc
      541 aataataaca ttcctccagt ggagtttaca caggaaaacc ccttttgccg atttaaggct
      601 gtcggttaca gttgtatgcc caataacaaa gatgaagatg gactagtggt tctaattttc
      661 aacaaaaaag aaacagatat tagaagccaa caacagcagt tggtagaatc attgcataaa
      721 gttctgggag gaaaccagac cctcactgtc aatgtggagg gcattaagac actgcccgac
      781 gatcagacag aagttgtcat ttacattgtt gagcgctctc caaatggcac ctcgagaaga
      841 gttccagcga caacgctgta tgcccacttt gagcaagcca acattaaaac acagcttcag
      901 cagcttggcg tgaccctttc tatgaccaga acagagctgt ctcctgctca gatcaagcag
      961 ctcctacaga accctcctgc tggtgttgat cccattattt gggaacaggc caaggtggat
     1021 aacccggatt ctgaaaaatt aattcctgtt ccaatggtgg gtttcaaaga attgcttcga
     1081 agactgaagg ttcaagacca gatgactaag cagcaccaga ccagattaga tatcatatct
     1141 gaagatatta gtgaactgca aaagaatcaa actacaacta tggccaaaat agcacaatac
     1201 aagaggaaac tgatggatct ctcacacaga accttacagg tcctaatcaa gcaggaaatc
     1261 cagaggaaga gtggctatgc catccaggct gaggaagaac agttgcgggt tcagctagat
     1321 acgattcaag gagagctgaa tgcacccact cagttcaaag gtcgactgaa tgaactgatg
     1381 tcccagatca ggatgcagaa tcattttgga gctgtgaaat ctgaagaaaa gtattacata
     1441 gatgcagacc tattacgaga gatcaagcag catttgaaac agcaacagga aggccttagc
     1501 cacctgatta gcatcataaa agacgacctg gaagatatca agctggtaga gcatggactg
     1561 aatgagacca tccacagcag aggcggggtc tttagctgac cattgcgctg tccagaggtt
     1621 cataaagctg tctgcttgct acgtcaggcc ttctttacga tgaccacatg gacaggaaaa
     1681 gggttctttt cctcactggg atgtttgagg ctcctcagaa attactgtac attaaatctg
     1741 aaatggtcac cacaaagctc ttcaaagaga gtctttgaaa agtttttgtc taaaagctgt
     1801 atttactttt tgaaaagtat tacccagagc ataaacactg ttgttcattt tataacagcg
     1861 ttaagtgtgg tcgtggggct tggtggtcat tctccactct tcccatagca caggatgagg
     1921 ccgagctgct tcctacgtaa cacgacaagc taatcccctg ccagttggag actgttcaga
     1981 gtctgttcag agtataaatg cgtctcagct tctatatctg tgaaatcagc tgtgggagta
     2041 aagaaaatcc caaagtattt aatgatttgt gtggttttcc ttttgttcta taaagacttg
     2101 aaaatatatt tttatattat tttacttatt tagaacttac agaaaatacc taaccagtta
     2161 ggatataaca aaatcaccac ttttgcaagc tttttttttt ttaattttta tttctaaaaa
     2221 tgaaatcctt aaaataaatt gttaaattgt aattttaaaa aaaaaaaaaa aaaaaaa
//
