LOCUS       NM_021663               1644 bp    mRNA    linear   ROD 12-AUG-2024
DEFINITION  Rattus norvegicus nucleobindin 2 (Nucb2), mRNA.
ACCESSION   NM_021663
VERSION     NM_021663.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Yu,H., Liu,Q., Xie,M., Fan,J., Luo,J., Huang,J. and Chen,L.
  TITLE     Nesfatin-1 inhibits cerebral aneurysms by activating Nrf2 and
            inhibiting NF-kappaB signaling
  JOURNAL   CNS Neurosci Ther 30 (8), e14864 (2024)
   PUBMED   39097921
  REMARK    GeneRIF: Nesfatin-1 inhibits cerebral aneurysms by activating Nrf2
            and inhibiting NF-kappaB signaling.
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Zhang,L., Zhuo,Z., Chen,L., Liu,J., Huang,J., Deng,J., Lu,W. and
            Jiang,X.
  TITLE     Nesfatin-1 alleviates hyperoxia-induced bronchopulmonary dysplasia
            (BPD) via the nuclear factor-kappaB (NF-kappaB) p65 signaling
            pathway
  JOURNAL   J Biochem Mol Toxicol 38 (4), e23680 (2024)
   PUBMED   38511245
  REMARK    GeneRIF: Nesfatin-1 alleviates hyperoxia-induced bronchopulmonary
            dysplasia (BPD) via the nuclear factor-kappaB (NF-kappaB) p65
            signaling pathway.
REFERENCE   3  (bases 1 to 1644)
  AUTHORS   Dang,Q., Zhu,Y., Zhang,Y., Hu,Z., Wei,Y., Chen,Z., Jiang,X., Cai,X.
            and Yu,H.
  TITLE     Nuclear Binding Protein 2/Nesfatin-1 Affects Trophoblast Cell
            Fusion during Placental Development via the EGFR-PLCG1-CAMK4
            Pathway
  JOURNAL   Int J Mol Sci 25 (3), 1925 (2024)
   PUBMED   38339201
  REMARK    GeneRIF: Nuclear Binding Protein 2/Nesfatin-1 Affects Trophoblast
            Cell Fusion during Placental Development via the EGFR-PLCG1-CAMK4
            Pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1644)
  AUTHORS   Lahane,G.P. and Dhar,A.
  TITLE     Nesfatin-1 peptide protects rat renal epithelial cells against high
            glucose and H2O2 induced injury via inhibition of oxidative stress,
            apoptosis, and fibrosis
  JOURNAL   Peptides 165, 171013 (2023)
   PUBMED   37105355
  REMARK    GeneRIF: Nesfatin-1 peptide protects rat renal epithelial cells
            against high glucose and H2O2 induced injury via inhibition of
            oxidative stress, apoptosis, and fibrosis.
REFERENCE   5  (bases 1 to 1644)
  AUTHORS   Li,Q., Wang,Y., Wei,R., An,Z., Dong,Z., Guan,Y. and Zhu,X.
  TITLE     A research on the expression of Nucb2/ cAMP / PKA in the
            hippocampus of alcohol-dependent rats
  JOURNAL   Biochem Biophys Res Commun 658, 148-152 (2023)
   PUBMED   37031608
  REMARK    GeneRIF: A research on the expression of Nucb2/ cAMP / PKA in the
            hippocampus of alcohol-dependent rats.
REFERENCE   6  (bases 1 to 1644)
  AUTHORS   Oh-I,S., Shimizu,H., Satoh,T., Okada,S., Adachi,S., Inoue,K.,
            Eguchi,H., Yamamoto,M., Imaki,T., Hashimoto,K., Tsuchiya,T.,
            Monden,T., Horiguchi,K., Yamada,M. and Mori,M.
  TITLE     Identification of nesfatin-1 as a satiety molecule in the
            hypothalamus
  JOURNAL   Nature 443 (7112), 709-712 (2006)
   PUBMED   17036007
  REMARK    GeneRIF: nesfatin, corresponding to NEFA/nucleobindin2 (NUCB2), a
            secreted protein of unknown function, is expressed in the
            appetite-control hypothalamic nuclei in rats
REFERENCE   7  (bases 1 to 1644)
  AUTHORS   Palmer,D.J., Kelly,V.C., Smit,A.M., Kuy,S., Knight,C.G. and
            Cooper,G.J.
  TITLE     Human colostrum: identification of minor proteins in the aqueous
            phase by proteomics
  JOURNAL   Proteomics 6 (7), 2208-2216 (2006)
   PUBMED   16502470
REFERENCE   8  (bases 1 to 1644)
  AUTHORS   Breuza,L., Halbeisen,R., Jeno,P., Otte,S., Barlowe,C., Hong,W. and
            Hauri,H.P.
  TITLE     Proteomics of endoplasmic reticulum-Golgi intermediate compartment
            (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies
            ERGIC-32, a new cycling protein that interacts with human Erv46
  JOURNAL   J Biol Chem 279 (45), 47242-47253 (2004)
   PUBMED   15308636
REFERENCE   9  (bases 1 to 1644)
  AUTHORS   Morel-Huaux,V.M., Pypaert,M., Wouters,S., Tartakoff,A.M.,
            Jurgan,U., Gevaert,K. and Courtoy,P.J.
  TITLE     The calcium-binding protein p54/NEFA is a novel luminal resident of
            medial Golgi cisternae that traffics independently of mannosidase
            II
  JOURNAL   Eur J Cell Biol 81 (2), 87-100 (2002)
   PUBMED   11893086
  REMARK    GeneRIF: Since NEFA is a peripheral luminal membrane constituent,
            its distinct trafficking from the transmembrane marker mannosidase
            II suggests a novel Golgi retention mechanism.
REFERENCE   10 (bases 1 to 1644)
  AUTHORS   Taniguchi,N., Taniura,H., Niinobe,M., Takayama,C.,
            Tominaga-Yoshino,K., Ogura,A. and Yoshikawa,K.
  TITLE     The postmitotic growth suppressor necdin interacts with a
            calcium-binding protein (NEFA) in neuronal cytoplasm
  JOURNAL   J Biol Chem 275 (41), 31674-31681 (2000)
   PUBMED   10915798
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC061778.1.
            
            On Dec 20, 2003 this sequence version replaced NM_021663.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061778.1, SRR8487230.112013.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q35"
     gene            1..1644
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /note="nucleobindin 2"
                     /db_xref="GeneID:59295"
                     /db_xref="RGD:620888"
     exon            1..52
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            53..213
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..1476
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /note="DNA-binding protein NEFA; nesfatin-1;
                     prepronefastin; prepronesfatin"
                     /codon_start=1
                     /product="nucleobindin-2 precursor"
                     /protein_id="NP_067695.1"
                     /db_xref="GeneID:59295"
                     /db_xref="RGD:620888"
                     /translation="MRWRTIQARYCFLLVPCVLTALEAVPIDVDKTKVHNVEPVESAR
                     IEPPDTGLYYDEYLKQVIEVLETDPHFREKLQKADIEEIRSGRLSQELDLVSHKVRTR
                     LDELKRQEVGRLRMLIKAKLDALQDTGMNHHLLLKQFEHLNHQNPDTFESKDLDMLIK
                     AATADLEQYDRTRHEEFKKYEMMKEHERREYLKTLSEEKRKEEEAKFAEMKRKHEDHP
                     KVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTKELDK
                     VYNPQNAEDDMIEMEEERLRMREHVMNEIDNNKDRLVTLEEFLRATEKKEFLEPDSWE
                     TLDQQQLFTEEELKEYESIIAIQESELKKKADELQKQKEELQRQHDHLEAQKQEYQQA
                     VQQLEQKKFQQGIAPSGPAGELKFEPHT"
     exon            214..357
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            358..465
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            466..592
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            593..696
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            697..882
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            883..973
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1032
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1125
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1215
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1386
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1468
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1617
                     /gene="Nucb2"
                     /gene_synonym="Nefa; p54"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcggcgggc atcagtcggc ttagcctcgc gcacggggac tctgcagaga agaacgcacc
       61 agtcagtttt tcaagggaaa aatctgggcc gggagccaag gcctgatcgc cacaaactta
      121 atattgtgta gattatttta ccacctgatc tcagtggcct catctgtgac aaaaagagag
      181 agaaaaagag ataaaacttt ggcctctctg aagatgaggt ggaggaccat ccaagcacgg
      241 tactgttttc tcttggttcc gtgcgtgctc actgcgctgg aagctgttcc tattgatgtg
      301 gacaagacca aagtgcacaa cgtcgagccg gtggaaagtg caaggataga accgccagac
      361 acgggacttt attatgatga atacctcaag caagtgattg aagtcttgga aacagatccg
      421 catttcagag aaaagctcca gaaagcagac atagaggaga taaggagcgg gaggctgagt
      481 caagagctgg acttagtaag tcacaaagtg aggacgagac tggatgaact gaagaggcaa
      541 gaagtaggaa gactgagaat gctcatcaaa gccaagctgg atgcccttca agacactggc
      601 atgaatcacc accttcttct taagcagttt gaacacctga accaccagaa tcctgacaca
      661 tttgaatcca aagacttgga tatgctaatc aaggcggcca ccgcggatct ggagcagtat
      721 gaccggactc ggcatgagga gtttaagaag tatgagatga tgaaggaaca tgaacgcaga
      781 gaatatttaa aaacgctgag tgaggagaag aggaaagagg aagaagccaa gtttgcagag
      841 atgaagagga agcatgaaga ccaccccaaa gttaatcacc ccggaagcaa agatcaacta
      901 aaagaggttt gggaagagac tgatggattg gaccctaatg actttgaccc caagacattt
      961 ttcaaattac atgatgttaa caacgatgga ttcctggatg aacaagaatt ggaagcactg
     1021 ttcacaaaag agttggacaa agtgtataac ccgcagaatg cagaggatga tatgatagaa
     1081 atggaagagg agaggctcag gatgagagag cacgtcatga atgagattga taacaacaaa
     1141 gaccgattgg tgactctgga ggaattcttg agagccacag agaagaaaga attcttggag
     1201 cccgatagct gggagacact ggaccagcag cagttattca ccgaggaaga gctcaaagag
     1261 tatgaaagta tcattgctat ccaagagagt gaacttaaga agaaggcaga tgaactgcag
     1321 aagcagaagg aggagctgca gcgccagcac gaccaccttg aggcccagaa gcaggagtat
     1381 cagcaggctg tacagcagct ggaacagaag aaattccaac aagggattgc tccatcaggg
     1441 ccggcaggag agctgaagtt tgagccacac acataaaagt cctgatgtct gccagaactt
     1501 ggaagaaaac cgttgactca acatctgttt catctttcaa catcccttct tttctcttca
     1561 ctcaataaat actttaaaag cacatttgga ataaaggaag agacttttaa gtgagaaaaa
     1621 aaaaaaaaaa aaaaaaaaaa aaaa
//
