LOCUS       NM_053463               2378 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus nucleobindin 1 (Nucb1), mRNA.
ACCESSION   NM_053463 XM_006229165
VERSION     NM_053463.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Tulke,S., Williams,P., Hellysaz,A., Ilegems,E., Wendel,M. and
            Broberger,C.
  TITLE     Nucleobindin 1 (NUCB1) is a Golgi-resident marker of neurons
  JOURNAL   Neuroscience 314, 179-188 (2016)
   PUBMED   26666627
  REMARK    GeneRIF: NUCB1 mRNA and protein was found to be present in all
            brain regions, extending to the spinal cord and dorsal root
            ganglia; the protein was detected in cell somata and proximal
            dendrites, but not in axons or terminal structures. Further
            examination of the subcellular distribution of NUCB1 using
            organelle-specific markers revealed its consistent presence in the
            Golgi apparatus.
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Palasz,A., Rojczyk,E., Bogus,K., Worthington,J.J. and
            Wiaderkiewicz,R.
  TITLE     The novel neuropeptide phoenixin is highly co-expressed with
            nesfatin-1 in the rat hypothalamus, an immunohistochemical study
  JOURNAL   Neurosci Lett 592, 17-21 (2015)
   PUBMED   25736948
  REMARK    GeneRIF: a wide distribution of phoenixin in the hypothalamus which
            could implicate a potential functional relationship with
            nesfatin-1, possibly in the regulation of the
            hypothalamic-pituitary-gonadal axis or other autonomic functions
REFERENCE   3  (bases 1 to 2378)
  AUTHORS   Zhang,W., Zhang,C., Fritze,D., Chai,B., Li,J. and Mulholland,M.W.
  TITLE     Modulation of food intake by mTOR signalling in the dorsal motor
            nucleus of the vagus in male rats: focus on ghrelin and nesfatin-1
  JOURNAL   Exp Physiol 98 (12), 1696-1704 (2013)
   PUBMED   23955309
  REMARK    GeneRIF: When administrated by injection into the fourth ventricle,
            nesfatin-1 suppressed food intake in comparison with the control
            vehicle.
REFERENCE   4  (bases 1 to 2378)
  AUTHORS   Garcia-Marcos,M., Kietrsunthorn,P.S., Wang,H., Ghosh,P. and
            Farquhar,M.G.
  TITLE     G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2
            (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs
  JOURNAL   J Biol Chem 286 (32), 28138-28149 (2011)
   PUBMED   21653697
  REMARK    GeneRIF: structural basis for the properties of Calnuc and NUCB2
            binding to Galpha subunits and its regulation by calcium ions.
REFERENCE   5  (bases 1 to 2378)
  AUTHORS   Kapoor,N., Gupta,R., Menon,S.T., Folta-Stogniew,E., Raleigh,D.P.
            and Sakmar,T.P.
  TITLE     Nucleobindin 1 is a calcium-regulated guanine nucleotide
            dissociation inhibitor of G{alpha}i1
  JOURNAL   J Biol Chem 285 (41), 31647-31660 (2010)
   PUBMED   20679342
  REMARK    GeneRIF: Nucleobindin 1 is a calcium-regulated guanine nucleotide
            dissociation inhibitor of G{alpha}i1.
REFERENCE   6  (bases 1 to 2378)
  AUTHORS   Lin,P., Fischer,T., Weiss,T. and Farquhar,M.G.
  TITLE     Calnuc, an EF-hand Ca(2+) binding protein, specifically interacts
            with the C-terminal alpha5-helix of G(alpha)i3
  JOURNAL   Proc Natl Acad Sci U S A 97 (2), 674-679 (2000)
   PUBMED   10639138
REFERENCE   7  (bases 1 to 2378)
  AUTHORS   Adams,L.D., Lemire,J.M. and Schwartz,S.M.
  TITLE     A systematic analysis of 40 random genes in cultured vascular
            smooth muscle subtypes reveals a heterogeneity of gene expression
            and identifies the tight junction gene zonula occludens 2 as a
            marker of epithelioid 'pup' smooth muscle cells and a participant
            in carotid neointimal formation
  JOURNAL   Arterioscler Thromb Vasc Biol 19 (11), 2600-2608 (1999)
   PUBMED   10559001
REFERENCE   8  (bases 1 to 2378)
  AUTHORS   Lin,P., Yao,Y., Hofmeister,R., Tsien,R.Y. and Farquhar,M.G.
  TITLE     Overexpression of CALNUC (nucleobindin) increases agonist and
            thapsigargin releasable Ca2+ storage in the Golgi
  JOURNAL   J Cell Biol 145 (2), 279-289 (1999)
   PUBMED   10209024
REFERENCE   9  (bases 1 to 2378)
  AUTHORS   Lin,P., Le-Niculescu,H., Hofmeister,R., McCaffery,J.M., Jin,M.,
            Hennemann,H., McQuistan,T., De Vries,L. and Farquhar,M.G.
  TITLE     The mammalian calcium-binding protein, nucleobindin (CALNUC), is a
            Golgi resident protein
  JOURNAL   J Cell Biol 141 (7), 1515-1527 (1998)
   PUBMED   9647645
REFERENCE   10 (bases 1 to 2378)
  AUTHORS   Wendel,M., Sommarin,Y., Bergman,T. and Heinegard,D.
  TITLE     Isolation, characterization, and primary structure of a
            calcium-binding 63-kDa bone protein
  JOURNAL   J Biol Chem 270 (11), 6125-6133 (1995)
   PUBMED   7890746
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On or before Nov 29, 2022 this sequence version replaced
            XM_006229165.2, NM_053463.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC100643.1, Z36277.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840115 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                JAXUCZ010000001.1  105125364-105125430 c
            68-209              JAXUCZ010000001.1  105124773-105124914 c
            210-317             JAXUCZ010000001.1  105116311-105116418 c
            318-450             JAXUCZ010000001.1  105114980-105115112 c
            451-554             JAXUCZ010000001.1  105112913-105113016 c
            555-740             JAXUCZ010000001.1  105111480-105111665 c
            741-831             JAXUCZ010000001.1  105111168-105111258 c
            832-890             JAXUCZ010000001.1  105107365-105107423 c
            891-983             JAXUCZ010000001.1  105107046-105107138 c
            984-1076            JAXUCZ010000001.1  105106880-105106972 c
            1077-1247           JAXUCZ010000001.1  105106465-105106635 c
            1248-1344           JAXUCZ010000001.1  105106002-105106098 c
            1345-2378           JAXUCZ010000001.1  105104793-105105826 c
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..2378
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /note="nucleobindin 1"
                     /db_xref="GeneID:84595"
                     /db_xref="RGD:620030"
     exon            1..67
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            68..209
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1457
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /note="CALNUC; bone 63 kDa calcium-binding protein"
                     /codon_start=1
                     /product="nucleobindin-1 precursor"
                     /protein_id="NP_445915.1"
                     /db_xref="GeneID:84595"
                     /db_xref="RGD:620030"
                     /translation="MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETP
                     DTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDEL
                     KRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTA
                     TRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKV
                     NVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVY
                     DPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKT
                     VEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAV
                     LQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPV
                     LPQLDSQHL"
     exon            210..317
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            318..450
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            451..554
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            555..740
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            741..831
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            832..890
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            891..983
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1076
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1247
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1344
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..2378
                     /gene="Nucb1"
                     /gene_synonym="Nucb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggagggaaa tcaccagaaa cctggcggag gtcgcgggct gggaaaacgc cctctgcgct
       61 aggagagacc agctacgatg cctacctctg tgccccgcgg ggcgcctttc cttctcctac
      121 cacctttact gatgctgtct gctgtgctgg cagtgcctgt ggaccgcgca gcacctcatc
      181 aggaggacaa ccaggccact gagaccccgg acacaggcct gtactaccat cggtacctcc
      241 aggaggtcat caacgtgcta gagacagatg ggcacttccg ggagaagctg caagctgcca
      301 acgctgagga cattaagagt ggaaagctga gtcaagagct ggacttcgtc agccacaacg
      361 tccgcaccaa gctggatgag ctcaagcgac aggaggtatc aaggcttcgg atgctgctca
      421 aggccaagat ggatgcaaag caggagccta acttgcaggt ggaccacatg aaccttctga
      481 agcagtttga acacctggac cctcagaacc agcacacgtt tgaggctcgg gacctagagc
      541 tgctgatcca gacggccacc cgagacctcg cccagtatga cgctgcacac catgaagagt
      601 tcaaacgcta cgagatgctc aaggaacatg agcgaagacg ttacctggag tctctgggag
      661 aggagcagcg gaaggaggct gagaggaagc tacaagagca gcagcgcaga caccgggaac
      721 accccaaagt caatgttcct ggcagccaag cccagttgaa ggaggtgtgg gaggagctag
      781 atggattgga ccccaacagg ttcaacccca agaccttctt catactgcat gacatcaaca
      841 gcgatggtgt cctagatgag caagaactgg aagctctgtt taccaaggag ctggagaagg
      901 tgtacgaccc gaagaacgag gaggacgaca tgagggagat ggaggaggag cggctgcgca
      961 tgcgggagca tgtgatgaag aacgtggaca ccaaccagga ccgacttgtg accctggagg
     1021 aattcctggc atccacacag aggaaggagt ttggggaaac tgcggaggga tggaagacag
     1081 tggaaatgta tccagcctac acagaggaag agctgaagcg ttttgaagag gagcttgctg
     1141 cccgggaggc tgagctaaat gccagagccc agcgcctcag ccaggagaca gaggccctgg
     1201 ggcgctccca ggaccgccta gaggctcaga agagagagct gcagcaggcc gttctgcaga
     1261 tggagcaaag gaagcagcaa cagcaagagc agagcgctcc gccttcccaa cctgatgggc
     1321 agctgcagtt ccgtgcagac acaggggatg ctcctgtccc agctccagca ggtgaccaga
     1381 aagacgtgcc tgcttctgaa aagaaggttc cagagcagcc ccctgtgctg ccacagctgg
     1441 attctcagca tttataagcc ctcagagatc ccggccctca agttcccaca gacagtgatg
     1501 gaagctggat gaagtgtcct tgcagccttt ctggagacca aagccttcat ggcctccagg
     1561 ggagaacagg gccaccctgt tcctcagatg cgcttctgcc catgtctacc agtctgttct
     1621 cacagcctct gggtcccagt cctgccttcc tccctccctt ccttggcagg gactcaactg
     1681 caggagcccc tcaccccacc tgagtgcaca ccccaggcct gcctgccttc ctgctttgct
     1741 cttacctgtc ccaggctcaa cctttctgct tcctgtacct gtcagtgggt cacaggagct
     1801 tgacttggtc tctccaactc tttgccttgt gagaggccaa tgatctcaat tccttttggt
     1861 ggctagaggc cgccacaatg tggggtgtcc tatcagctct gcctgggact cctgaactcc
     1921 accaccaccc caccagcctt accctaacca cctgggccca atacctacct gagggtccca
     1981 gaaggggccc agatatggct acacccctta ctgaggtcca gcctcactca gccccctatg
     2041 ggggctcctg aaaattggtt cacccacttc cctggcttca ggccacactg ctgtgtcctg
     2101 ctctgtgtcc cagtctcatc caactatagc ctggcacaaa taaacattag catatctgtg
     2161 tgaatgtact gccctcttgc aagtccccct gcatgccacc tgctcgactc cctggtgtcc
     2221 tgtggcacat acttccactg ccaggaggct tgccgggggc tttctgtgtg ctcttgccag
     2281 aatggcctcc ttgcttcatt tttgccatgg aaacagaaaa cccaaacctg agcttgaaaa
     2341 aaaattaata tcttaaataa ttaaaatata tatatgta
//
