LOCUS       NM_001399578            1324 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus 5',3'-nucleotidase, mitochondrial (Nt5m), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001399578 XM_006246460
VERSION     NM_001399578.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   3  (bases 1 to 1324)
  AUTHORS   Rinaldo-Matthis,A., Rampazzo,C., Reichard,P., Bianchi,V. and
            Nordlund,P.
  TITLE     Crystal structure of a human mitochondrial deoxyribonucleotidase
  JOURNAL   Nat Struct Biol 9 (10), 779-787 (2002)
   PUBMED   12352955
REFERENCE   4  (bases 1 to 1324)
  AUTHORS   Gallinaro,L., Crovatto,K., Rampazzo,C., Pontarin,G., Ferraro,P.,
            Milanesi,E., Reichard,P. and Bianchi,V.
  TITLE     Human mitochondrial 5'-deoxyribonucleotidase. Overproduction in
            cultured cells and functional aspects
  JOURNAL   J Biol Chem 277 (38), 35080-35087 (2002)
   PUBMED   12124385
REFERENCE   5  (bases 1 to 1324)
  AUTHORS   Rampazzo,C., Kost-Alimova,M., Ruzzenente,B., Dumanski,J.P. and
            Bianchi,V.
  TITLE     Mouse cytosolic and mitochondrial deoxyribonucleotidases: cDNA
            cloning of the mitochondrial enzyme, gene structures, chromosomal
            mapping and comparison with the human orthologs
  JOURNAL   Gene 294 (1-2), 109-117 (2002)
   PUBMED   12234672
REFERENCE   6  (bases 1 to 1324)
  AUTHORS   Rampazzo,C., Gallinaro,L., Milanesi,E., Frigimelica,E., Reichard,P.
            and Bianchi,V.
  TITLE     A deoxyribonucleotidase in mitochondria: involvement in regulation
            of dNTP pools and possible link to genetic disease
  JOURNAL   Proc Natl Acad Sci U S A 97 (15), 8239-8244 (2000)
   PUBMED   10899995
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Jan 12, 2022 this sequence version replaced XM_006246460.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC167058.1, SRR22582419.1327263.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-307               JAXUCZ010000010.1  45152505-45152811
            308-408             JAXUCZ010000010.1  45156061-45156161
            409-469             JAXUCZ010000010.1  45164421-45164481
            470-584             JAXUCZ010000010.1  45176706-45176820
            585-1324            JAXUCZ010000010.1  45177948-45178687
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..1324
                     /gene="Nt5m"
                     /note="5',3'-nucleotidase, mitochondrial"
                     /db_xref="GeneID:287368"
                     /db_xref="RGD:1309622"
     exon            1..307
                     /gene="Nt5m"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..727
                     /gene="Nt5m"
                     /EC_number="3.1.3.5"
                     /codon_start=1
                     /product="5'(3')-deoxyribonucleotidase, mitochondrial"
                     /protein_id="NP_001386507.1"
                     /db_xref="GeneID:287368"
                     /db_xref="RGD:1309622"
                     /translation="MLRLRGCCARPRGAPLRAERSRASSRALRVLVDMDGVLADFEGG
                     FLRKFRARFPDLPFVALEDRRGFWVSEQYGRLQPGLSEKAISIWESKDFFFELEPLPG
                     AVEAVKHMASLQNTDVFICTSPIKMFKYCPYEKYAWVEKHFGPDFLEQIVLTRDKTVV
                     SADLLIDDRPDITGAESHPSWEHILFTSCHNYHLQLQPPRRRLHSWADDWKAILDSKR
                     PR"
     exon            308..408
                     /gene="Nt5m"
                     /inference="alignment:Splign:2.1.0"
     exon            409..469
                     /gene="Nt5m"
                     /inference="alignment:Splign:2.1.0"
     exon            470..584
                     /gene="Nt5m"
                     /inference="alignment:Splign:2.1.0"
     exon            585..1324
                     /gene="Nt5m"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcctgcgcg tctgtccgcg gaggaggctg cgtctgccca gggccttggg cgaggagcgg
       61 ggcgatgctc aggctgcgcg gctgctgcgc gcgtccccgc ggtgccccgc tacgagctga
      121 gcggagccgg gcgagcagcc gcgccctgcg ggtgctagtg gacatggacg gcgtgcttgc
      181 ggactttgag ggcggcttcc tccggaagtt ccgcgcgcgc ttcccggacc tgcccttcgt
      241 ggcgctggag gaccggcggg gcttctgggt gtcggagcag tacggacgtc tgcagcctgg
      301 gctgagcgag aaggccatca gcatctggga gtcgaaggat ttcttctttg aacttgagcc
      361 ccttcctgga gctgtggaag ctgtgaagca tatggccagt ctgcagaaca ccgatgtttt
      421 catctgtacg agccccatca agatgttcaa gtactgtccc tatgagaagt atgcctgggt
      481 ggagaagcac tttggccctg actttctaga acaaattgtg ttgaccagag acaagaccgt
      541 ggtctctgcc gaccttctca tagatgaccg gccagacatc acaggggccg agtcacaccc
      601 cagctgggag cacatcctct tcacctcctg ccacaactac cacctgcagc tgcagccccc
      661 tcgccgcagg ctgcactcgt gggcagatga ctggaaggcc atcttggaca gcaaacggcc
      721 acgctgaggg ctgtgctgca cctgtctggt ggtccagtgt ggggctcaca gctcatgact
      781 ggacagcaaa caccccgctg agggctgtgc tgcagtttct ggtacttccc agaggaccca
      841 gtgaacactt cggtcaaaac aacttccgac acacgtccct tctcccccta gcccagcaag
      901 gccttaacct ggtattggga cagggctgga agaaaggcac gcactccaga caagttggtc
      961 tgacctcagg cagaggctcc ccaccactgc ctctcactgc gcacaatagg tgtgttctct
     1021 gagacgtgag agtaccctgg tccaggacct gcaaggcgtg gagtgccagg gatagatgac
     1081 agaggttccc tcggcaggca tgtgtccctc tacctctact gtagaatctc gtccagccta
     1141 cctactcagt ctctgtggct tgccaaggcc accaggctct tttggaggcc aacagtgacc
     1201 ctgcctatgg ccagtgccat ggtctcttgc tctggtgtcc ctagatccca ttcatggccc
     1261 agaaaggagt cagcaggctc gaatatttgt tgacgacatg agaaataaat gctgttcaca
     1321 ttta
//
