LOCUS       NM_021576               2826 bp    mRNA    linear   ROD 07-APR-2024
DEFINITION  Rattus norvegicus 5' nucleotidase, ecto (Nt5e), mRNA.
ACCESSION   NM_021576
VERSION     NM_021576.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Aoki,T., Wong,V., Yin,T., Nakamura,E., Endo,Y., Hayashida,K.,
            Robson,S.C., Nandurkar,H., Diamond,B., Kim,S.J., Murao,A., Wang,P.,
            Becker,L.B. and Shinozaki,K.
  TITLE     Immune cell expression patterns of CD39/CD73 ectonucleotidases in
            rodent models of cardiac arrest and resuscitation
  JOURNAL   Front Immunol 15, 1362858 (2024)
   PUBMED   38545102
  REMARK    GeneRIF: Immune cell expression patterns of CD39/CD73
            ectonucleotidases in rodent models of cardiac arrest and
            resuscitation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Adzic Bukvic,M., Laketa,D., Dragic,M., Lavrnja,I. and
            Nedeljkovic,N.
  TITLE     Expression of functionally distinct ecto-5'-nucleotidase/CD73
            glycovariants in reactive astrocytes in experimental autoimmune
            encephalomyelitis and neuroinflammatory conditions in vitro
  JOURNAL   Glia 72 (1), 19-33 (2024)
   PUBMED   37646205
  REMARK    GeneRIF: Expression of functionally distinct
            ecto-5'-nucleotidase/CD73 glycovariants in reactive astrocytes in
            experimental autoimmune encephalomyelitis and neuroinflammatory
            conditions in vitro.
REFERENCE   3  (bases 1 to 2826)
  AUTHORS   Carmo,M., Goncalves,F.Q., Canas,P.M., Oses,J.P., Fernandes,F.D.,
            Duarte,F.V., Palmeira,C.M., Tome,A.R., Agostinho,P., Andrade,G.M.
            and Cunha,R.A.
  TITLE     Enhanced ATP release and CD73-mediated adenosine formation sustain
            adenosine A2A receptor over-activation in a rat model of
            Parkinson's disease
  JOURNAL   Br J Pharmacol 176 (18), 3666-3680 (2019)
   PUBMED   31220343
  REMARK    GeneRIF: Enhanced ATP release and CD73-mediated adenosine formation
            sustain adenosine A2A receptor over-activation in a rat model of
            Parkinson's disease.
REFERENCE   4  (bases 1 to 2826)
  AUTHORS   Chen,X., Zeng,M., He,D., Yan,X., Chen,H., Chen,Y., Xia,C., Wang,J.,
            Shen,L., Zhu,D. and Wang,J.
  TITLE     Asthmatic Augmentation of Airway Vagal Activity Involves Decreased
            Central Expression and Activity of CD73 in Rats
  JOURNAL   ACS Chem Neurosci 10 (6), 2809-2822 (2019)
   PUBMED   30913879
  REMARK    GeneRIF: in experimental asthma, decreased CD73 expression and
            activity in airway vagal centers contribute to the augmentation of
            airway vagal activity
REFERENCE   5  (bases 1 to 2826)
  AUTHORS   Cardoso,A.M., Manfredi,L.H., Zanini,D., Bagatini,M.D.,
            Gutierres,J.M., Carvalho,F., Tremblay,A., Bello-Klein,A.,
            Rubin,M.A., Morsch,V.M., Sevigny,J. and Schetinger,M.R.C.
  TITLE     Physical exercise prevents memory impairment in an animal model of
            hypertension through modulation of CD39 and CD73 activities and A2A
            receptor expression
  JOURNAL   J Hypertens 37 (1), 135-143 (2019)
   PUBMED   30507864
  REMARK    GeneRIF: Studied effect of exercise on memory and cerebral cortex
            physiology of NTPDase-1 (CD39), 5' nucleotidase ecto (CD73), and
            adenosine A2a receptor (A2ar).
REFERENCE   6  (bases 1 to 2826)
  AUTHORS   Fuchs,J.L.
  TITLE     5'-Nucleotidase activity increases in aging rat brain
  JOURNAL   Neurobiol Aging 12 (5), 523-530 (1991)
   PUBMED   1770988
REFERENCE   7  (bases 1 to 2826)
  AUTHORS   Ogata,S., Hayashi,Y., Misumi,Y. and Ikehara,Y.
  TITLE     Membrane-anchoring domain of rat liver 5'-nucleotidase:
            identification of the COOH-terminal serine-523 covalently attached
            with a glycolipid
  JOURNAL   Biochemistry 29 (34), 7923-7927 (1990)
   PUBMED   2148114
REFERENCE   8  (bases 1 to 2826)
  AUTHORS   Misumi,Y., Ogata,S., Ohkubo,K., Hirose,S. and Ikehara,Y.
  TITLE     Primary structure of human placental 5'-nucleotidase and
            identification of the glycolipid anchor in the mature form
  JOURNAL   Eur J Biochem 191 (3), 563-569 (1990)
   PUBMED   2129526
REFERENCE   9  (bases 1 to 2826)
  AUTHORS   Misumi,Y., Ogata,S., Hirose,S. and Ikehara,Y.
  TITLE     Primary structure of rat liver 5'-nucleotidase deduced from the
            cDNA. Presence of the COOH-terminal hydrophobic domain for possible
            post-translational modification by glycophospholipid
  JOURNAL   J Biol Chem 265 (4), 2178-2183 (1990)
   PUBMED   2298743
REFERENCE   10 (bases 1 to 2826)
  AUTHORS   Song,C.S. and Bodansky,O.
  TITLE     Subcellular localization and properties of 5'-nucleotidase in the
            rat liver
  JOURNAL   J Biol Chem 242 (4), 694-699 (1967)
   PUBMED   6017738
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC098665.1.
            
            On Jun 10, 2007 this sequence version replaced NM_021576.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098665.1, J05214.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q31"
     gene            1..2826
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /note="5' nucleotidase, ecto"
                     /db_xref="GeneID:58813"
                     /db_xref="RGD:61956"
     exon            1..380
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1766
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.91"
                     /EC_number="3.1.3.89"
                     /EC_number="3.1.3.99"
                     /EC_number="3.1.3.35"
                     /note="5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase;
                     5'-deoxynucleotidase; thymidylate 5'-phosphatase;
                     IMP-specific 5'-nucleotidase"
                     /codon_start=1
                     /product="5'-nucleotidase precursor"
                     /protein_id="NP_067587.2"
                     /db_xref="GeneID:58813"
                     /db_xref="RGD:61956"
                     /translation="MRPAAATAPKWLLLALSALLPLWPTAKSWELTILHTNDVHSRLE
                     QTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLE
                     VAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAPQISGLY
                     LPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLKTLNVNKII
                     ALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRK
                     VPVVQAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLD
                     NYSTQELGRTIVYLNGSAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVN
                     GGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGE
                     FLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLEMDKVYKVVLPSYLVNGGD
                     GFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIKFSAASHYQGSFPLIILS
                     FWAVILVLYQ"
     exon            381..603
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            604..792
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            793..990
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1145
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1251
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1401
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1602
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..2826
                     /gene="Nt5e"
                     /gene_synonym="CD73; Nt5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggccagttca cccgctcaac gcgctcctgc cagccatgcg tcccgcggct gctacggcac
       61 ccaagtggct gcttctcgcg ctaagcgctc tactaccact gtggcccaca gccaaatcct
      121 gggagctcac gatcctgcac acaaacgacg tgcacagccg gctagagcaa accagcgatg
      181 actccaccaa gtgcctcaac gcaagcctgt gtgtgggcgg cgtggcccgg ctcttcacca
      241 aggtgcagca gatccgcaag gaagaaccca acgtactgct tttggatgct ggcgatcagt
      301 accagggcac catctggttc actgtttaca aaggcctgga agtggcacac ttcatgaacc
      361 tcctgggcta cgatgccatg gcactgggaa atcatgaatt tgataacggt gtggaaggac
      421 tgattgatcc cctcctcaga aatgtcaaat ttccaattct gagtgcaaat attaaggccc
      481 gggggccact agcacctcag atatccggac tttatttgcc atataaagtt ctctctgtcg
      541 gtggtgaggt tgtggggatt gttggatata cttcaaagga aacccccttc ctctcaaatc
      601 cagggacaaa tttagtcttc gaagatgaag tcactgcgtt gcagcctgaa gtggataaac
      661 taaaaactct aaacgtgaac aagatcattg ccctgggaca ctctggtttc gagatggata
      721 aactcattgc ccagaaggtg agaggcgtgg acgtcgtggt gggaggacac accaacacct
      781 ttctctacac aggaaatcca ccttccaaag aagtgcctgc tgggaagtac ccattcatag
      841 tcacctctga cgatgggcga aaggttcccg tggtccaggc ctatgccttt ggcaaatacc
      901 tgggctatct gaaggttgag tttgatgata aaggaaatgt tgtcacttcc tatggaaatc
      961 ccattcttct gaacagcacc attcgtgaag atgcagccat caaagcagac attaaccagt
     1021 ggaggataaa attagataat tattctacac aggaactcgg gagaaccatc gtctacctga
     1081 atggctccgc tcaggagtgc cgcttcagag agtgcaacat gggaaacctg atctgtgatg
     1141 ccatgattaa caacaacctc agacacccag atgaaatgtt ttggaaccac gtgtccatgt
     1201 gcattgtaaa tggaggtggc atccggtccc ccattgatga gaggaacaat ggtaccatca
     1261 cctgggagaa cctggctgct gtgctgccct ttggagggac atttgacctt gtccaattga
     1321 aagggtccac cctgaagaag gcttttgagc acagtgtgca tcgatatggc cagtccacag
     1381 gggagttcct gcaagtgggt ggaatccatg tggtgtatga tatttcccga aagccctggg
     1441 acagagtggt ccaattaaaa gttctctgca ccaagtgtcg agtgcccatc tatgagcctc
     1501 tcgaaatgga taaagtgtat aaagtggtcc tcccaagcta tctggtcaac ggtggggatg
     1561 gattccaaat gataaaagat gaattattaa aacatgactc tggtgaccaa gatatcagtg
     1621 tggtttctga atacatctca aaaatgaaag taatttaccc agcagttgaa ggacggatca
     1681 agttctctgc agcaagtcat taccaaggaa gctttccttt aataattctt tccttttggg
     1741 cagtgatcct tgttttgtac caataacagg aagtcttgtc cttggtgtca aactgcattt
     1801 ttcttccagt gatattcaaa tctgcctctg gaaagctggc tttgtgatgg tgctcatcat
     1861 cctcaaggct ccaggcagat gctcttcaca aggaagagac tgtaatatca tttgttggga
     1921 ccagcaactc aatgagcaga gagtcaaagt gaaccaacag ggtccttctg gaaggtagtg
     1981 ggtaggggaa acatctgggt gtagtttgca catccacata acacatctgg ctaccactga
     2041 gcctcaaaaa taatttttcc ctttctattc atttctaatc catcaaacaa ctgatgttta
     2101 catagaactt tatcattgcc agttctggtg gcacatgccc gtggtcacag aacttgggag
     2161 ggaggagagg atggctgcaa gttctaggcc agcctgacct atgtagagtt tcaagccagt
     2221 tagctagaca tcaagactca cacaaacaaa caaaaccatt ataatttaca agtagatttc
     2281 tgtagacaag tattgtgatg ttaatcagaa agggttgact tgttcaaggc cataaatctc
     2341 taaacaatac atctaggacc tgaacccaag tctttttacc tcaagtccaa tactctctcc
     2401 tacagtcaag tctcctctct tcctgccaat ggccccagat gacaaatctc tgtttcagcc
     2461 ctccatacta tcctttcttt tgggctccta ttgtctctca agtttgagag agtaactact
     2521 ggacaggaca tgtgcctttg atgagtcaca gacaaactgt ataaagcaga taatgggtta
     2581 gtccagggaa tgcaaaaggc agtcagggac agtggagaaa gggaaaggag aatgtgacca
     2641 ggactgatac attatagaag agagaatggg tatttagatg tgttacacaa acactagtta
     2701 agaagcaggg cataggggct ggggatttag ctcagtggta gagcgcttac ctaggaagcg
     2761 caaggccctg ggttcggtcc ccagctccga aaaaaagaac caaaaaaaaa aaaaaaaaaa
     2821 aaaaaa
//
