LOCUS       NM_001401711            2607 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus 5'-nucleotidase, cytosolic II (Nt5c2), mRNA.
ACCESSION   NM_001401711 XM_006231586
VERSION     NM_001401711.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Mirzaee,S., Eriksson,S. and Albertioni,F.
  TITLE     Differences in cytosolic and mitochondrial 5'-nucleotidase and
            deoxynucleoside kinase activities in Sprague-Dawley rat and CD-1
            mouse tissues: implication for the toxicity of nucleoside analogs
            in animal models
  JOURNAL   Toxicology 267 (1-3), 159-164 (2010)
   PUBMED   19913594
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wallden,K., Stenmark,P., Nyman,T., Flodin,S., Graslund,S.,
            Loppnau,P., Bianchi,V. and Nordlund,P.
  TITLE     Crystal structure of human cytosolic 5'-nucleotidase II: insights
            into allosteric regulation and substrate recognition
  JOURNAL   J Biol Chem 282 (24), 17828-17836 (2007)
   PUBMED   17405878
REFERENCE   3  (bases 1 to 2607)
  AUTHORS   Mackiewicz,M., Nikonova,E.V., Zimmerman,J.E., Galante,R.J.,
            Zhang,L., Cater,J.R., Geiger,J.D. and Pack,A.I.
  TITLE     Enzymes of adenosine metabolism in the brain: diurnal rhythm and
            the effect of sleep deprivation
  JOURNAL   J Neurochem 85 (2), 348-357 (2003)
   PUBMED   12675911
REFERENCE   4  (bases 1 to 2607)
  AUTHORS   Spychala,J., Madrid-Marina,V. and Fox,I.H.
  TITLE     High Km soluble 5'-nucleotidase from human placenta. Properties and
            allosteric regulation by IMP and ATP
  JOURNAL   J Biol Chem 263 (35), 18759-18765 (1988)
   PUBMED   2848805
REFERENCE   5  (bases 1 to 2607)
  AUTHORS   Itoh,R.
  TITLE     Purification and some properties of cytosol 5'-nucleotidase from
            rat liver
  JOURNAL   Biochim Biophys Acta 657 (2), 402-410 (1981)
   PUBMED   6260203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Feb 22, 2022 this sequence version replaced XM_006231586.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.5662.1,
                                           SRR26643299.7552.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760386,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                JAXUCZ010000001.1  255787231-255787267 c
            38-157              JAXUCZ010000001.1  255774875-255774994 c
            158-231             JAXUCZ010000001.1  255748570-255748643 c
            232-349             JAXUCZ010000001.1  255730260-255730377 c
            350-445             JAXUCZ010000001.1  255729211-255729306 c
            446-537             JAXUCZ010000001.1  255725145-255725236 c
            538-595             JAXUCZ010000001.1  255724965-255725022 c
            596-689             JAXUCZ010000001.1  255723940-255724033 c
            690-743             JAXUCZ010000001.1  255722876-255722929 c
            744-827             JAXUCZ010000001.1  255720952-255721035 c
            828-869             JAXUCZ010000001.1  255720119-255720160 c
            870-977             JAXUCZ010000001.1  255719085-255719192 c
            978-1044            JAXUCZ010000001.1  255718797-255718863 c
            1045-1215           JAXUCZ010000001.1  255718017-255718187 c
            1216-1267           JAXUCZ010000001.1  255716348-255716399 c
            1268-1328           JAXUCZ010000001.1  255716173-255716233 c
            1329-1505           JAXUCZ010000001.1  255715869-255716045 c
            1506-2607           JAXUCZ010000001.1  255713909-255715010 c
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q54"
     gene            1..2607
                     /gene="Nt5c2"
                     /note="5'-nucleotidase, cytosolic II"
                     /db_xref="GeneID:100363253"
                     /db_xref="RGD:2323387"
     exon            1..37
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            38..157
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..1739
                     /gene="Nt5c2"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.99"
                     /EC_number="2.7.1.77"
                     /note="cytosolic nucleoside phosphotransferase 5'N"
                     /codon_start=1
                     /product="cytosolic purine 5'-nucleotidase"
                     /protein_id="NP_001388640.1"
                     /db_xref="GeneID:100363253"
                     /db_xref="RGD:2323387"
                     /translation="MMTSWSDRLQNAADVPANMDKHALKKYRREAYHRVFVNRSLAME
                     KIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLNFAYDSTFPTRGLVF
                     DTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLP
                     ETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKT
                     VENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSH
                     RPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDT
                     ICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEEL
                     QSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLF
                     ASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRN
                     RTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEE"
     exon            158..231
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            232..349
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            350..445
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            446..537
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            538..595
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            596..689
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            690..743
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            744..827
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            828..869
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            870..977
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1044
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1215
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1267
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1328
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1505
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..2607
                     /gene="Nt5c2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agttgcgcgc taggattgct gccgtgcggt ggggccgaat tcatcctaca aataaaatga
       61 tgacttcctg gagtgaccgc ttacagaacg cagcagatgt gcctgctaac atggataagc
      121 atgccctgaa aaagtaccgt cgagaagcct atcaccgggt gtttgtgaac cgaagtttag
      181 ccatggaaaa aataaagtgt tttgggtttg acatggatta taccctggct gtgtacaagt
      241 ctccggaata tgaatccctt ggctttgagc ttactgtgga gagattagtg tccatcggct
      301 atcctcagga gctgctcaac tttgcatatg attcgacatt ccctaccaga ggacttgtct
      361 ttgacacact ctatggaaat ctcttgaaag ttgatgccta tggaaacctc ttggtttgtg
      421 cacatggatt taacttcata agaggaccag aaactagaga gcagtatcca aataagttta
      481 tccaacgaga tgatactgaa cggttttaca ttctgaacac actcttcaac cttccagaaa
      541 cctacctgtt ggcctgccta gtagattttt ttactaattg tcccagatat accagttgtg
      601 acacaggatt taaagatggg gacctcttca tgtcctacag gagtatgttc caggatgtaa
      661 gggacgcagt ggactgggtc cattacaagg gttcccttaa ggaaaagaca gttgaaaatc
      721 ttgagaagta tgtagtcaaa gatggcaagt tgccgttgct tctgagccga atgaaggaag
      781 tggggaaagt gtttctggcc actaacagtg actataagta cacagataaa atcatgactt
      841 acctgtttga tttcccacat ggccccaagc ctgggagctc ccaccggccg tggcagtcct
      901 actttgatct gattttggtg gatgcacgga aaccgctctt ttttggagaa ggcacagtgc
      961 tgcgtcaggt ggacactaaa accggaaaac tgaaaatcgg cacctacacg ggccccctgc
     1021 agcatggcat cgtctactca ggaggttcat ctgatacaat ctgtgacctg ttgggagcta
     1081 agggcaaaga cattttgtat attggagatc acatttttgg agacattttg aagtcaaaga
     1141 aacggcaagg gtggagaact ttcttggtga ttcctgagct tgcacaagag ctgcacgtct
     1201 ggactgacaa aagttcactt tttgaagaac ttcaaagctt ggatattttc ttggctgaac
     1261 tctacaagca cctggacagc agcagcaacg agcgcccaga catcagttcc atccagagac
     1321 ggatcaagaa agtgactcat gacatggaca tgtgctacgg gatgatggga agcctgttcc
     1381 gcagtggctc ccggcagacg ctctttgcca gccaagtgat gcggtacgct gacctctatg
     1441 cagcatcctt catcaacctg ctgtactacc cattcagcta cctcttcaga gcggcccacg
     1501 tcctgatgcc gcacgaatca acagtggagc acacacatgt ggatatcaat gagatggagt
     1561 cccctctcgc cacccgcaac cgcacatcgg tggatttcaa agacactgac tataagcgac
     1621 accagctcac gaggtccatt agtgagatca agcctcccaa cctcttccct ctggctcccc
     1681 aggagatcac acactgccat gatgaagatg atgatgagga agaggaagag gaagaataag
     1741 gcagactgaa aaccctgagc acccatcaag tctgacggac tcgtgggggc aaggggcaga
     1801 ttgtttggat tgttagggag ggtgggagga ctccatcaga agcacgtttg gaaagtacgt
     1861 ttctgaaggc tttaaataat tctaatcata gctacttcag ttttgtgtat ctgtattctc
     1921 tgcagatggt ttgaaactgc ataggaagag caggtaaagt caggtggaat gcatgcagac
     1981 ggcaccatgt ggcttgggat ggtttctgtg cctgtcgtcg tgtcatctgg atgcacgaag
     2041 gtagtaaaag gttcagagca gaggggctga tgggaagagg agacccacaa atgctgtgta
     2101 caaactgtac cattgctcca atcccacccg gcctgtgcgt cacagttgct caccagtgga
     2161 gaatacacac ttttatcacc aagtgtagtc agcctctgtt gaggtcaagt ttccctccgt
     2221 tagcttttgg gtttcctcgg ccctggcgag gctggtgctg tgtcagtcag tgcctttgtg
     2281 ggtttccttg ctgtaggacg attctagact gcagggattt gaatttgagg caaaagtcct
     2341 catccttgct acatttttta aagttggtag cacaaatgct ttgtgttaca taaaaccaat
     2401 ccttttgggg aatgtagctg aggagtacaa aggagcaggt tcccagaaca gcagccactg
     2461 tcacagccag agctgccttc agcagcgtgg cctgctccca gccacagaga cgggaaggct
     2521 ctacagcaga agggacagag gggatgagtt cactcgactg ggaaatgacc tctggctctg
     2581 aataaagcag gagcctgtaa acacgaa
//
