LOCUS       NM_001108403            4664 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus NOP2/Sun RNA methyltransferase 2 (Nsun2), mRNA.
ACCESSION   NM_001108403 XM_001057743 XM_341474
VERSION     NM_001108403.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Shinoda S, Kitagawa S, Nakagawa S, Wei FY, Tomizawa K, Araki K,
            Araki M, Suzuki T and Suzuki T.
  TITLE     Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial
            tRNAs
  JOURNAL   Nucleic Acids Res 47 (16), 8734-8745 (2019)
   PUBMED   31287866
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Van Haute L, Lee SY, McCann BJ, Powell CA, Bansal D, Vasiliauskaite
            L, Garone C, Shin S, Kim JS, Frye M, Gleeson JG, Miska EA, Rhee HW
            and Minczuk M.
  TITLE     NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs
  JOURNAL   Nucleic Acids Res 47 (16), 8720-8733 (2019)
   PUBMED   31276587
REFERENCE   3  (bases 1 to 4664)
  AUTHORS   Chen X, Li A, Sun BF, Yang Y, Han YN, Yuan X, Chen RX, Wei WS, Liu
            Y, Gao CC, Chen YS, Zhang M, Ma XD, Liu ZW, Luo JH, Lyu C, Wang HL,
            Ma J, Zhao YL, Zhou FJ, Huang Y, Xie D and Yang YG.
  TITLE     5-methylcytosine promotes pathogenesis of bladder cancer through
            stabilizing mRNAs
  JOURNAL   Nat Cell Biol 21 (8), 978-990 (2019)
   PUBMED   31358969
REFERENCE   4  (bases 1 to 4664)
  AUTHORS   Wang N, Tang H, Wang X, Wang W and Feng J.
  TITLE     Homocysteine upregulates interleukin-17A expression via
            NSun2-mediated RNA methylation in T lymphocytes
  JOURNAL   Biochem Biophys Res Commun 493 (1), 94-99 (2017)
   PUBMED   28919411
  REMARK    GeneRIF: In conclusion, NSun2 mediates HHcy-induced upregulation of
            IL-17A expression by methylating IL-17A mRNA and promoting its
            translation in T lymphocytes.
REFERENCE   5  (bases 1 to 4664)
  AUTHORS   Yang X, Yang Y, Sun BF, Chen YS, Xu JW, Lai WY, Li A, Wang X,
            Bhattarai DP, Xiao W, Sun HY, Zhu Q, Ma HL, Adhikari S, Sun M, Hao
            YJ, Zhang B, Huang CM, Huang N, Jiang GB, Zhao YL, Wang HL, Sun YP
            and Yang YG.
  TITLE     5-methylcytosine promotes mRNA export - NSUN2 as the
            methyltransferase and ALYREF as an m5C reader
  JOURNAL   Cell Res 27 (5), 606-625 (2017)
   PUBMED   28418038
REFERENCE   6  (bases 1 to 4664)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y,
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E,
            Bonneau R, Selbach M, Dieterich C and Landthaler M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   7  (bases 1 to 4664)
  AUTHORS   Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein
            C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J and
            Hentze MW.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   8  (bases 1 to 4664)
  AUTHORS   Zhang X, Liu Z, Yi J, Tang H, Xing J, Yu M, Tong T, Shang Y,
            Gorospe M and Wang W.
  TITLE     The tRNA methyltransferase NSun2 stabilizes p16INK mRNA by
            methylating the 3'-untranslated region of p16
  JOURNAL   Nat Commun 3, 712 (2012)
   PUBMED   22395603
  REMARK    Erratum:[Nat Commun. 2013;4:2703]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4664)
  AUTHORS   Blanco S, Kurowski A, Nichols J, Watt FM, Benitah SA and Frye M.
  TITLE     The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells
            to differentiate
  JOURNAL   PLoS Genet 7 (12), e1002403 (2011)
   PUBMED   22144916
REFERENCE   10 (bases 1 to 4664)
  AUTHORS   Brzezicha B, Schmidt M, Makalowska I, Jarmolowski A, Pienkowska J
            and Szweykowska-Kulinska Z.
  TITLE     Identification of human tRNA:m5C methyltransferase catalysing
            intron-dependent m5C formation in the first position of the
            anticodon of the pre-tRNA Leu (CAA)
  JOURNAL   Nucleic Acids Res 34 (20), 6034-6043 (2006)
   PUBMED   17071714
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474002.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_341474.3, XM_001057743.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1p11"
     gene            1..4664
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /note="NOP2/Sun RNA methyltransferase 2"
                     /db_xref="GeneID:361191"
                     /db_xref="RGD:1311954"
     exon            1..173
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     CDS             3..2351
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /EC_number="1.3.99.5"
                     /EC_number="2.1.1.29"
                     /note="tRNA (cytosine-5-)-methyltransferase NSUN2;
                     NOL1/NOP2/Sun domain family, member 2; tRNA
                     (cytosine(34)-C(5))-methyltransferase; NOP2/Sun RNA
                     methyltransferase family, member 2"
                     /codon_start=1
                     /product="RNA cytosine C(5)-methyltransferase NSUN2"
                     /protein_id="NP_001101873.1"
                     /db_xref="GeneID:361191"
                     /db_xref="RGD:1311954"
                     /translation="MQPEVRRVCCAVGSCVRSACAAAWAMGRRARGRRFQQPPQPEGE
                     EDGSDGSRKRGQEGWEGGYPEIIKENKLFEHYYQELKIVPEGEWDQFMGSLREPLPAT
                     LRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKI
                     LRKSPLLAKFHQFLVSETESGNISRQEAVSMIPPLLLNVEPHHKILDMCAAPGSKTTQ
                     LIEMLHADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLTV
                     DVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQ
                     LAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSAELPGLKWMPGVSQWKVM
                     TRDGQWFADWQEVPQGRHTQIRPTMFPPKDLDKLQAMHLERCLRILPHHQNTGGFFVA
                     VLVKKAPMPWNKRQPKVQSKSAQARDPKASNTVAATKGNPSDQSELESQMITGAGDSE
                     AAQTTENTESNEKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPS
                     FPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCA
                     FRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENPFFRKLSSEAYSQVKDLTKGSVV
                     LKYEPDSANPDTLRCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVI
                     LTNESVASPEQPEEEDAKQTAQDPCNPDSVPGCDPGAAEPSR"
     exon            174..331
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            332..436
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            437..542
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            543..614
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            615..699
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            700..892
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            893..967
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1098
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1172
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1303
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1400
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1582
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1675
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1811
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1892
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2031
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2071
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..4664
                     /gene="Nsun2"
                     /gene_synonym="RGD1311954"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggatgcagcc ggaagtacgg cgtgtgtgct gtgcggtggg tagctgtgtg cgctccgcgt
       61 gtgctgccgc gtgggccatg gggcggcggg cgcggggccg gcggttccag cagccgccgc
      121 agcctgaggg cgaggaagac ggcagcgacg gcagcagaaa gcgcggccag gagggctggg
      181 aaggtggata tcccgagatc ataaaggaga acaagctctt tgagcactac tatcaggaac
      241 tcaagatcgt gccggaggga gaatgggacc aattcatggg ctcacttcga gagcccctcc
      301 cagccacatt gagaatcact ggttacaaaa gccatgcaaa agagattctc cattgcttga
      361 agaacaagta ttttaaggaa ttggaggacc tggaggtaga tggacagaaa gttgaagttc
      421 ctcagccact aagctggtac cctgaagaac ttgcctggca cacaaattta agtcggaaaa
      481 tcttgagaaa gtccccattg ttggcaaagt ttcatcagtt cctggtcagc gagacggagt
      541 ctggaaacat cagccgccag gaggctgtca gcatgattcc cccactgctg ctcaacgttg
      601 agccccatca taagatctta gacatgtgtg cagcccctgg atccaagacc acacagttaa
      661 ttgaaatgtt gcatgcagac atgagtgtgc cctttccaga gggatttgta atcgcaaatg
      721 acgtggacaa caagcgctgc tatctgctcg tccatcaggc caaacggttg agcagtccct
      781 gcatcatggt ggtgaaccat gacgcatcca gcattcctag actcacagta gatgtggatg
      841 gaaggaaaga gattctcttc tatgatcgga ttttgtgtga tgtcccttgc agtggcgatg
      901 gcacaatgag aaaaaacatt gatgtctgga agaaatggac taccttaaac agcttgcagc
      961 tccatggcct acagcttcgg attgcaaccc gaggtgctga gcagctggcc gagggtggca
     1021 gaatggtgta ttccacctgt tccttgaacc ctgtggaaga tgaagccgtc atagcagctc
     1081 tgctggagaa gagtgaaggt gccctggagc ttgctgatgt atctgctgag ttgccaggac
     1141 taaagtggat gcctggagtc tcacagtgga aggtcatgac tagagatggg cagtggtttg
     1201 cagactggca ggaggttccc cagggcaggc acacgcaaat acggcccacc atgttccccc
     1261 caaaggacct ggacaaacta caggcaatgc atttagagcg atgccttcga atactgcccc
     1321 atcatcagaa tactggaggg ttctttgtgg cagtattggt caagaaagca ccaatgccat
     1381 ggaacaaacg tcagcccaag gtacagagta aatctgcaca ggccagagac cccaaagcat
     1441 ccaacactgt ggctgccaca aagggaaatc ccagtgacca gtctgagctg gaaagccaga
     1501 tgatcactgg agctggtgac tcagaagcag ctcagacgac tgagaacaca gagagtaatg
     1561 agaagaaaga tggcgtatgt ggccctcctc catcaaagaa aatgaagttg tttggattca
     1621 aagaagatcc gttcgtgttc attcctgagg atgatccttt atttccacct attgagaagt
     1681 tctatgcctt ggatccttca ttcccgagga tgaatttgct aacccgaacc acagaaggaa
     1741 agaagcggca gctttatatg gtctccaagg agttgaggaa tgtactgctc aacaacagcg
     1801 agaagatgaa ggtcattaac actgggataa aagtctggtg tcgaaataac agtggtgaag
     1861 agtttgactg tgcgttccgt ttggcacagg agggaatata tacattgtat ccatttatca
     1921 attcaagaat cattactgta tcaatggaag atgtgaagac actgttgacc caggagaacc
     1981 cattctttag aaaactgagc agtgaggcct acagtcaagt taaggacctc acaaagggaa
     2041 gtgttgtgct gaagtacgaa ccagattctg cgaatccaga caccctccgg tgccccattg
     2101 tgctatgtgg gtggcgggga aaggcctcta ttagaacttt tgtgcccaaa aatgagcggc
     2161 tgcattatct caggatgatg ggtctagagg tattgggaga aaagaaaaaa gagggtgtca
     2221 ttcttaccaa tgagagtgtt gccagcccag aacagcctga agaagaggat gccaagcaga
     2281 cagcacaaga cccctgtaac ccagactctg ttcctggatg tgacccaggt gcagctgagc
     2341 catcccggtg aagatgtccc acacactgaa ttggtaccat ccttttgttg gagatcaaac
     2401 ctagaatgtc cttagctggg acctggagat gagtgggtgg ctagtctagg gagagctata
     2461 ttttctagca tttggaagtc gcttttgcag aatggattca tgttcctgct ttaaaggcta
     2521 atatgacaca gcctttgcag aagtcgcttt tgcagagtgg atacatgttt ctgctttaaa
     2581 ggctaatatg acacagcctt tgcagaagtt cggatccctt gtcgggccaa tattggccta
     2641 gtctaaaatt atagcaaaaa tctacaagtc ttgggaagca actctatgct tttaatattt
     2701 tttgggctgc acatcagtgt gctgtgcagt cacgctgaca ctgaggtctc aggtgacttc
     2761 tgtgcctttg tgataaaggg tgagggtaag cctggtgaat catgacagca gttttgggga
     2821 ccgtttgtgc ttatcctgga agtgctttaa gtggggtgga gattgctggg tcatgaaata
     2881 aagagctatt ataatgtgtc ttgacatatg acttggattt gcttttagtt ggctaatggg
     2941 agagagagaa ccaagccctc ttgtgtctca tataggaatt gaagctgggc tgctgcagtg
     3001 ttgtcagcca ctgtgtcctg agcagcttcc tgctacagtg aggtgaggca gatggacctg
     3061 ggctgtgctg gatcctagac tggctgtagc acagacccaa gctcatgccc actgagccat
     3121 tgatgtctca gcatttaagg gtgaagaagt agagctgggt ggacctgcac ttgtgtcccc
     3181 tgcctggctt ccaggagtgc tagcctggcc ggccacgttt atcctcctca gcagtgtccc
     3241 ccatcagcct catgctcaga gtttggttat cattgaacac aaatgtaggg cagacgtatc
     3301 cagtgacgtc tctgtattcg tgtcctcttg aaacattttc ctgtcagctg ctgtgttccc
     3361 cctcacccag attttccggg cagagttctt gcaggtgctc tttcttccct gtgtatgtaa
     3421 tatgttttgc ctgcactgtt gagcattaat tacatgcatg ttctccaggc cttcctatat
     3481 atgctctgag ccaaacacca gctatctctg ggatagctgt gccttgctca taaaagttca
     3541 atccagttgg atctgttgac tgtttatccc ctgaggagag acataggatc ctcacctgaa
     3601 tgtccagctg cgccatacca caagtatgta actctgcctt ggggagggcc agaggataaa
     3661 gaagaccagg gtgggaatcc catctatgtg gctatgggga agccatcttc tggttgaaga
     3721 cttgggctac aaaagactgt cttgcagggg aaggaaaccc aagtccctgt aagtagccct
     3781 cacctgtcct acaagtaagc cctgtaattc accggttccc cagaatgaat tctggtagaa
     3841 gtgtggggtt tagagaaagc atggtgttgg gctctaagga ggaggggcac actttgctta
     3901 cttccctgtc caggggaggg agacatttta gcagtagtgg gtctctccta acataggtga
     3961 gcctgacctt gagaagacca cttagtgaga agacctctaa gctgtagcca ttggaagtac
     4021 ctcactgccg agtagcctgg tgacaacctc agccagtgct gccctggatg cctccatctt
     4081 gtgagtcctt ctcttacagg atgtgggttc gttactttgt agagtgttct cgggctcttc
     4141 cttcttacta gagcaggtag gcagtgcagc tagggccgtg gcactgttgg ttgtctgtca
     4201 ccttgtggca gctttgcttg ttggcagaca gctacctgcc attgtgccca tcccgtgttt
     4261 gtgtattata gggaagtccc ttcaagactt tgttcttttt cctcagactt caacagtcag
     4321 catagtttct gagacgtcat tttgttaaat aagttatgaa cctgagaacc tcctgcttca
     4381 gcctcccaag tactgttttc cccttgtttc ctctgaattt aggagtggat ggccatgagt
     4441 tactatgtcc agctttggat gccgtcttgt agagaccatc taagcagatg aaactgtctg
     4501 attcttggtc aaatcctact ggacgtgcag gtcaggtggc agtcctgggg ccacatagac
     4561 gtggaaccat tcaggctgtg tgcattaacc ttgggtccta ggatagttaa agtgcttata
     4621 gtgtagtatg ttttcttgtc aatttcagta agggttgtct gatt
//
