LOCUS       NM_181389               3547 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus neutral sphingomyelinase activation associated
            factor (Nsmaf), mRNA.
ACCESSION   NM_181389 XM_039110148
VERSION     NM_181389.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Frazziano,G., Moreno,L., Moral-Sanz,J., Menendez,C., Escolano,L.,
            Gonzalez,C., Villamor,E., Alvarez-Sala,J.L., Cogolludo,A.L. and
            Perez-Vizcaino,F.
  TITLE     Neutral sphingomyelinase, NADPH oxidase and reactive oxygen
            species. Role in acute hypoxic pulmonary vasoconstriction
  JOURNAL   J Cell Physiol 226 (10), 2633-2640 (2011)
   PUBMED   21792922
  REMARK    GeneRIF: We propose an integrated signalling pathway for hypoxic
            pulmonary vasoconstriction which includes nSMase-PKCzeta-NADPH
            oxidase as a necessary step required for reactive oxygen species
            production.
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Lei,X., Zhang,S., Bohrer,A., Bao,S., Song,H. and Ramanadham,S.
  TITLE     The group VIA calcium-independent phospholipase A2 participates in
            ER stress-induced INS-1 insulinoma cell apoptosis by promoting
            ceramide generation via hydrolysis of sphingomyelins by neutral
            sphingomyelinase
  JOURNAL   Biochemistry 46 (35), 10170-10185 (2007)
   PUBMED   17685585
  REMARK    GeneRIF: These findings indicate that iPLA2beta activation
            participates in ER stress-induced INS-1 cell apoptosis by promoting
            ceramide generation via NSMase-catalyzed hydrolysis of
            sphingomyelins
REFERENCE   3  (bases 1 to 3547)
  AUTHORS   O'Brien,N.W., Gellings,N.M., Guo,M., Barlow,S.B., Glembotski,C.C.
            and Sabbadini,R.A.
  TITLE     Factor associated with neutral sphingomyelinase activation and its
            role in cardiac cell death
  JOURNAL   Circ Res 92 (6), 589-591 (2003)
   PUBMED   12637370
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On or before Mar 2, 2021 this sequence version replaced
            XM_039110148.1, NM_181389.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.12685.1,
                                           SRR26643296.14674.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-275               JAXUCZ010000005.1  24375081-24375355   c
            276-365             JAXUCZ010000005.1  24362183-24362272   c
            366-444             JAXUCZ010000005.1  24358241-24358319   c
            445-512             JAXUCZ010000005.1  24357921-24357988   c
            513-549             JAXUCZ010000005.1  24357806-24357842   c
            550-600             JAXUCZ010000005.1  24354739-24354789   c
            601-672             JAXUCZ010000005.1  24353673-24353744   c
            673-720             JAXUCZ010000005.1  24353257-24353304   c
            721-773             JAXUCZ010000005.1  24353108-24353160   c
            774-903             JAXUCZ010000005.1  24348231-24348360   c
            904-975             JAXUCZ010000005.1  24346223-24346294   c
            976-1084            JAXUCZ010000005.1  24344271-24344379   c
            1085-1261           JAXUCZ010000005.1  24342851-24343027   c
            1262-1341           JAXUCZ010000005.1  24340659-24340738   c
            1342-1432           JAXUCZ010000005.1  24339665-24339755   c
            1433-1496           JAXUCZ010000005.1  24339481-24339544   c
            1497-1548           JAXUCZ010000005.1  24337243-24337294   c
            1549-1669           JAXUCZ010000005.1  24337032-24337152   c
            1670-1801           JAXUCZ010000005.1  24336562-24336693   c
            1802-1844           JAXUCZ010000005.1  24336008-24336050   c
            1845-2008           JAXUCZ010000005.1  24335374-24335537   c
            2009-2117           JAXUCZ010000005.1  24333886-24333994   c
            2118-2176           JAXUCZ010000005.1  24332480-24332538   c
            2177-2244           JAXUCZ010000005.1  24327660-24327727   c
            2245-2312           JAXUCZ010000005.1  24326882-24326949   c
            2313-2436           JAXUCZ010000005.1  24326371-24326494   c
            2437-2520           JAXUCZ010000005.1  24324587-24324670   c
            2521-2671           JAXUCZ010000005.1  24317565-24317715   c
            2672-2774           JAXUCZ010000005.1  24317148-24317250   c
            2775-2884           JAXUCZ010000005.1  24316925-24317034   c
            2885-3547           JAXUCZ010000005.1  24315319-24315981   c
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q12"
     gene            1..3547
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /note="neutral sphingomyelinase activation associated
                     factor"
                     /db_xref="GeneID:353233"
                     /db_xref="RGD:727868"
     exon            1..275
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..2979
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /note="factor associated with neutral sphingomyelinase
                     activation; neutral sphingomyelinase (N-SMase) activation
                     associated factor"
                     /codon_start=1
                     /product="protein FAN"
                     /protein_id="NP_852054.2"
                     /db_xref="GeneID:353233"
                     /db_xref="RGD:727868"
                     /translation="MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQ
                     GSPEERKIRGSLKICSKSVIFEPDAISQPILKIPLRDCLKIGKHGENGANKHFAKAKS
                     LGISLIFSQVYFIKEHNIVAPYKIERGKMEYVFQLEVSGKVEDVVETLLQLHRASCLD
                     KLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMVTPLVTNPGHVCITDT
                     NLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQ
                     DRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYP
                     VFPWVISDYSSPELDLSNPATFRDLSKPVGALNPERLERLLTRYQEMPEPKFMYGSHY
                     SSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFY
                     DEDASFLINSLKLDLGKRQGGQMVDDVELPAWASSPQDFLQKNKDALESSYVSEHLHE
                     WIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDDKVAMLTQILEFGQTPKQ
                     LFVTPHPRRITPKFKSLSQASSYNASMTDSPVSPGEESFEDLTEESKTLAWNNITKLQ
                     LHEQYKIHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCL
                     LLPGDTTVISSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSASWDSTVK
                     VWSGVPAEMPGTKRHQFDLLAELEHDVSVNTINLNAVSTLLVSGTKEGLVNIWDLTTA
                     TLLHQISCHSGTVCDAAFSPDSRHVLSTGVDGCLNVIDVQTGMLISSMASEEPQRCFV
                     WDGNSVLSGSRTGELLVWDLLGAKVSERIQGHTGAVTCIWMNEQCSSIITGGEDRQVM
                     FWKLQY"
     exon            276..365
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            366..444
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            445..512
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            513..549
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            550..600
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            601..672
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            673..720
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            721..773
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            774..903
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            904..975
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1084
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1261
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1341
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1432
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1496
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1548
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1669
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1801
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1844
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2008
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2117
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2176
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2244
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2245..2312
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2436
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2520
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2521..2671
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2774
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2775..2884
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3547
                     /gene="Nsmaf"
                     /gene_synonym="FAN"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgtgggac gcggctcccg cggcctcagg tcacggcgcg cccctcctcc gggcctgagt
       61 cacggcgtcg ccggagtccc cacgcgagga tgctgcggtg agcgaggtcc ggacgccgcg
      121 ggccctgggg cgcagcgtgg ggctctcggc cgcccgccca gcgccgccgc tcgggactgc
      181 tcgggacagt cggcggcctg cgcgccccgg gcctccatgg cgttcacccg gaagaggcag
      241 cgcgagcagc agctgcagct ctactccaag gagaggttct ccttactgct gctcaacttg
      301 gaggagtact acttcgagca gcatacggct ttccatgtcc agcaccaggg cagccccgag
      361 gaaaggaaaa tcagaggctc cttaaagata tgttcaaaat cagtgatttt tgaaccagat
      421 gcaatatcgc agcccattct taagattcct ttgagagact gtttaaaaat aggaaaacat
      481 ggagaaaatg gagccaataa acactttgca aaggcaaaat ctttgggtat ttcactcatt
      541 ttcagtcagg tatatttcat taaggagcac aacatcgtgg caccgtataa gatagaacgg
      601 ggaaaaatgg agtatgtctt ccaactggaa gtttctggga aggtggaaga tgttgtggag
      661 acattgctac agctgcacag agcatcctgc cttgataaac ttggtgacca aatggccatg
      721 ataacagcta tcttgcagtc acgtttggct aggacatcat ttgacaaaaa caggttccaa
      781 agcgtttcag aaaagctgca catggagtgc aaagcagaga tggtgacgcc tctggtgacg
      841 aaccctgggc atgtgtgcat tactgacacc aacctgtatt tccagcctct caacgggtac
      901 ccgaaacctg tggtccagat aacgctccaa gatgtccggc gcatttacaa aaggaggcac
      961 ggcctcatgc ctttgggctt ggaagtgttt tgcacagaag atgacctgtg ttctgacata
     1021 tacctaaagt tctatgagcc tcaagacaga gatgacctct atttttatat tgccacgtac
     1081 ctagaacacc acgtggcaga acacactgcg gagagctaca tgctgcagtg gcagcgaggg
     1141 cacctgtcca actaccagta cctgctccac ctcaacaacc tggccgaccg cagctgcaat
     1201 gacctgtccc agtaccctgt gtttccatgg gtaataagtg actactccag cccagagcta
     1261 gatttgtcaa atcctgcaac cttccgagat cttagtaagc cagtgggagc cctaaatcca
     1321 gagaggctgg agagactgct gactcgatac caggaaatgc ctgagccgaa gttcatgtat
     1381 gggagccact actcttcccc aggctatgtg ctcttttacc tcgttaggat cgcaccagaa
     1441 tatatgctgt gtctacaaaa tggaagattt gataatgcag ataggatgtt caacagcatt
     1501 gcagaaacct ggaaaaactg tttggatggt gcgacggact tcaaagagtt gatcccagaa
     1561 ttctatgatg aagacgccag cttcttgatc aatagtctga agttggatct ggggaagaga
     1621 caaggagggc agatggttga cgacgtggag cttcctgcat gggcctcaag tccccaagac
     1681 tttctccaga agaacaagga cgccttggaa agcagttatg tgtctgagca ccttcatgag
     1741 tggattgatc tgatatttgg ctataagcaa aaagggagtg aggccatagg ggcccataac
     1801 gtgtttcacc ccctgaccta tgaaggcggt gtagacctga acagcattga ggatcccgat
     1861 gacaaagtag ccatgctgac ccaaattttg gaatttgggc agacaccaaa acagctgttt
     1921 gtgacaccac atcctcgaag gatcaccccc aagtttaaaa gtttgtccca agcgtccagc
     1981 tacaatgctt ctatgacaga ctccccagtt tctccagggg aggagtcatt tgaagacctg
     2041 actgaagaga gcaaaactct ggcctggaat aacatcacca agttgcagct acatgagcag
     2101 tacaaaatcc acaaagaggc ggttacaggg atagcagtgt cttgcaatgg gtcttcagtc
     2161 tttaccacct cacaagattc cactttgaag atgttttcta aggaatccaa aatgttacaa
     2221 agaagtatat cattttcaaa tatggcttta tcgtcttgtt tacttttgcc aggagacacc
     2281 actgtcataa gttcttcttg ggataacaac gtctattttt attccatagc atttggaaga
     2341 cgccaagaca cactgatggg acatgacgat gctgtcagta agatctgttg gcataatgac
     2401 aggctgtact ctgcatcttg ggactctact gtgaaggtgt ggtccggcgt tcctgcagaa
     2461 atgccaggca caaaaagaca ccagtttgac ttactggctg agcttgagca tgatgtcagt
     2521 gtcaatacga tcaatttaaa tgctgtgagt acactgctag tttctggcac caaggaaggc
     2581 ctggtgaata tttgggacct cactacagcc actctattac accagatttc atgccactca
     2641 gggacagtat gtgatgctgc ttttagccca gacagtcgcc acgtcctcag cacaggagta
     2701 gacggctgcc tgaacgtgat tgatgtgcag acgggcatgc tcatttcctc catggcctca
     2761 gaggagccgc agaggtgctt tgtctgggat ggaaactctg tcttatctgg aagtcggact
     2821 ggtgagctgc ttgtctggga cctccttgga gcaaaagtca gtgagcggat acagggccac
     2881 acaggtgccg tgacgtgcat atggatgaat gaacagtgta gcagcatcat caccggaggg
     2941 gaagacagac aagttatgtt ctggaaactg cagtattaag tgccttttcc tctctatcaa
     3001 gttcaattct atttaatgta tttttaaacc aaacttttaa ctggtgaatg tgcaatgata
     3061 gtacttagaa gtttgaccac atgaagaatt tgtggttcca acttcctaaa tcatggtgac
     3121 ttcattcaga gccatttgtt gccatccatt aagcagataa aacacagccg tgctagggga
     3181 aaggaaattc taagacagag gaaggactgt cacacatgac aacacttaga agagtacggg
     3241 gtggttcagg acaagaaaga tacccagttg gactgaggaa cattggacaa cactggccag
     3301 ctcagtcccc aagaggcgaa gcagcctctg acctaagctg cccaggtctg atgataacac
     3361 cgttcttcgt gtgtttcatg gactcgttcc tttctgtggt gctgtgtgcc cagcttctga
     3421 gaatctgggg gaggagtagc tgttcttata acaggaagtc tattactgtg tatagcaatg
     3481 gtttatattt cattatgcta cgaaatgttc atcttttcta cattaaaaat atttttctgt
     3541 aatgaaa
//
