LOCUS       NM_021748               3752 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus N-ethylmaleimide sensitive factor, vesicle fusing
            ATPase (Nsf), mRNA.
ACCESSION   NM_021748
VERSION     NM_021748.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Das,D., Bao,H., Courtney,K.C., Wu,L. and Chapman,E.R.
  TITLE     Resolving kinetic intermediates during the regulated assembly and
            disassembly of fusion pores
  JOURNAL   Nat Commun 11 (1), 231 (2020)
   PUBMED   31932584
  REMARK    GeneRIF: Once trans-SNARE complexes that form large, stable pores
            by Ca2+-synaptotagmin 1, they can only be closed by the action of
            the N-ethylmaleimide sensitive factor(NSF). Time-resolved
            measurements reveal that NSF-mediated pore closure occurs via a
            complex 'stuttering' mechanism.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Xiong,H., Casse,F., Zhou,M., Xiong,Z.Q., Joels,M., Martin,S. and
            Krugers,H.J.
  TITLE     Interactions between N-Ethylmaleimide-sensitive factor and GluA2
            contribute to effects of glucocorticoid hormones on AMPA receptor
            function in the rodent hippocampus
  JOURNAL   Hippocampus 26 (7), 848-856 (2016)
   PUBMED   26766634
  REMARK    GeneRIF: Results indicate that glucocorticoid hormones increase
            AMPA receptor mediated synaptic transmission and synaptic insertion
            of AMPA receptors via a mechanism that requires NSF/GluA2
            interaction. This may contribute to the memory enhancing effects of
            glucocorticoid hormones.
REFERENCE   3  (bases 1 to 3752)
  AUTHORS   Gomez-Chacon,B., Gamiz,F., Foster,T.C. and Gallo,M.
  TITLE     Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala
            and Perirhinal Cortex during Habituation of Taste Neophobia
  JOURNAL   Neural Plast 2016, 2726745 (2016)
   PUBMED   26839712
  REMARK    GeneRIF: We have found increased expression of NSF gene in
            basolateral amygdala and perirhinal cortex in groups of rats
            undergoing taste habituation.
REFERENCE   4  (bases 1 to 3752)
  AUTHORS   Qu,Y., Ma,K.N. and Li,X.Z.
  TITLE     Identification of differentially expressed proteins and validation
            of the changes of N-ethylmaleimide-sensitive factor in rats with
            focal cerebral ischemia after transection of the cervical
            sympathetic trunk
  JOURNAL   J Huazhong Univ Sci Technolog Med Sci 34 (6), 801-807 (2014)
   PUBMED   25480573
  REMARK    GeneRIF: TCST increases the expression of NSF in the hippocampus of
            adult rats with focal cerebral ischemia, which may contribute to
            the protection of the injured brain.
REFERENCE   5  (bases 1 to 3752)
  AUTHORS   Migues,P.V., Hardt,O., Finnie,P., Wang,Y.W. and Nader,K.
  TITLE     The maintenance of long-term memory in the hippocampus depends on
            the interaction between N-ethylmaleimide-sensitive factor and GluA2
  JOURNAL   Hippocampus 24 (9), 1112-1119 (2014)
   PUBMED   24753224
  REMARK    GeneRIF: Results indicate that the persistence of long-term memory
            depends on the maintenance of a steady-state level of synaptic
            GluA2/AMPARs, which requires the interaction of NSF with GluA2
REFERENCE   6  (bases 1 to 3752)
  AUTHORS   Nishimune,A., Isaac,J.T., Molnar,E., Noel,J., Nash,S.R., Tagaya,M.,
            Collingridge,G.L., Nakanishi,S. and Henley,J.M.
  TITLE     NSF binding to GluR2 regulates synaptic transmission
  JOURNAL   Neuron 21 (1), 87-97 (1998)
   PUBMED   9697854
REFERENCE   7  (bases 1 to 3752)
  AUTHORS   Matveeva,E.A., He,P. and Whiteheart,S.W.
  TITLE     N-Ethylmaleimide-sensitive fusion protein contains high and low
            affinity ATP-binding sites that are functionally distinct
  JOURNAL   J Biol Chem 272 (42), 26413-26418 (1997)
   PUBMED   9334216
REFERENCE   8  (bases 1 to 3752)
  AUTHORS   Hanson,P.I., Roth,R., Morisaki,H., Jahn,R. and Heuser,J.E.
  TITLE     Structure and conformational changes in NSF and its membrane
            receptor complexes visualized by quick-freeze/deep-etch electron
            microscopy
  JOURNAL   Cell 90 (3), 523-535 (1997)
   PUBMED   9267032
REFERENCE   9  (bases 1 to 3752)
  AUTHORS   Perier,F., Coulter,K.L., Liang,H., Radeke,C.M., Gaber,R.F. and
            Vandenberg,C.A.
  TITLE     Identification of a novel mammalian member of the
            NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in
            yeast
  JOURNAL   FEBS Lett 351 (2), 286-290 (1994)
   PUBMED   8082782
REFERENCE   10 (bases 1 to 3752)
  AUTHORS   Johnson,M.N. and Okunade,A.O.
  TITLE     Roles that nurses in the community can play in nursing research
  JOURNAL   Int Nurs Rev 22 (5), 147-149 (1975)
   PUBMED   1041498
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Nov 24, 2020 this sequence version replaced NM_021748.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ211783.1, SRR26360186.1469896.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                JAXUCZ010000010.1  89357326-89357383   c
            59-144              JAXUCZ010000010.1  89335446-89335531   c
            145-244             JAXUCZ010000010.1  89333775-89333874   c
            245-284             JAXUCZ010000010.1  89331158-89331197   c
            285-451             JAXUCZ010000010.1  89322128-89322294   c
            452-559             JAXUCZ010000010.1  89318720-89318827   c
            560-635             JAXUCZ010000010.1  89317728-89317803   c
            636-791             JAXUCZ010000010.1  89315263-89315418   c
            792-991             JAXUCZ010000010.1  89284270-89284469   c
            992-1157            JAXUCZ010000010.1  89275772-89275937   c
            1158-1232           JAXUCZ010000010.1  89275224-89275298   c
            1233-1420           JAXUCZ010000010.1  89274768-89274955   c
            1421-1516           JAXUCZ010000010.1  89266588-89266683   c
            1517-1673           JAXUCZ010000010.1  89265754-89265910   c
            1674-1807           JAXUCZ010000010.1  89262594-89262727   c
            1808-1874           JAXUCZ010000010.1  89253684-89253750   c
            1875-1954           JAXUCZ010000010.1  89252054-89252133   c
            1955-2089           JAXUCZ010000010.1  89233973-89234107   c
            2090-2203           JAXUCZ010000010.1  89232683-89232796   c
            2204-2259           JAXUCZ010000010.1  89229855-89229910   c
            2260-3752           JAXUCZ010000010.1  89227937-89229429   c
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.1"
     gene            1..3752
                     /gene="Nsf"
                     /note="N-ethylmaleimide sensitive factor, vesicle fusing
                     ATPase"
                     /db_xref="GeneID:60355"
                     /db_xref="RGD:621594"
     exon            1..58
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..2281
                     /gene="Nsf"
                     /EC_number="3.6.4.6"
                     /note="NEM-sensitive fusion protein;
                     N-ethylmaleimide-sensitive factor; vesicular-fusion
                     protein NSF; N-ethylmaleimide-sensitive fusion protein"
                     /codon_start=1
                     /product="vesicle-fusing ATPase"
                     /protein_id="NP_068516.2"
                     /db_xref="GeneID:60355"
                     /db_xref="RGD:621594"
                     /translation="MAGRTMQAARCPTDELSLSNCAVVNEKDYQSGQHVMVRTSPNHK
                     YIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFL
                     QKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSIL
                     KGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKM
                     GIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKM
                     LNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAIC
                     KQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVK
                     MEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQS
                     TAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGII
                     KWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIK
                     ICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQ
                     ALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALEL
                     LGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASP
                     LDFD"
     exon            59..144
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            145..244
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            245..284
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            285..451
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            452..559
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            560..635
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            636..791
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            792..991
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1157
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1232
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1420
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1516
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1673
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1807
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1874
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1954
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2089
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2203
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2259
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..3752
                     /gene="Nsf"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcagagtgc ggagctgcag cctgggagca ggacgtgtcc gcgaagatgg cgggccggac
       61 tatgcaagct gcgagatgcc ctactgatga attgtctcta agcaactgtg cagttgtgaa
      121 cgaaaaggat taccagtctg gccagcatgt gatggtgagg acgtccccca accacaagta
      181 catatttacg ctgcggaccc atccgtccgt ggttcccgga tgcatcgcgt tcagcttacc
      241 ccagcgaaaa tgggctggac tttctattgg gcaggacata gaagtcgcct tgtattcgtt
      301 tgacaaagcc aagcagtgca tcggcacaat gaccatcgag atcgacttcc tgcagaaaaa
      361 gaacatcgac tccaaccctt acgacaccga caagatggct gccgagttca tccagcagtt
      421 caacaaccag gccttctcag tgggacagca gcttgtcttt agcttcaatg ataagctctt
      481 tggattgctg gtgaaggaca tcgaagccat ggatcccagc atcctgaagg gagagcctgc
      541 gtcaggtaaa aggcagaaga ttgaagtagg actggtcgtt ggaaacagtc aagttgcatt
      601 tgaaaaagca gaaaactcat cactcaatct tattggcaag gctaaaacca aggaaaatcg
      661 ccagtctatc atcaatcccg actggaactt tgagaaaatg gggatcggcg gtctggataa
      721 ggagttttca gatattttcc gacgagcatt tgcttctcgg gtgttccctc cggagatcgt
      781 ggaacaaatg ggttgcaaac atgttaaagg catcctgtta tacggacccc ctggttgtgg
      841 taagactctc ttggctcgac agattggcaa gatgctgaat gcaagagagc ccaaggtggt
      901 caatgggccg gaaatcctta acaaatatgt gggagaatca gaggccaaca tccgcaagct
      961 ctttgctgat gcagaagagg agcagagacg gctcggtgcc aacagcggcc tgcacatcat
     1021 catctttgat gaaatcgacg ccatctgcaa gcagagaggg agcatggccg gcagcaccgg
     1081 ggtgcacgac actgtggtca accagctgct gtccaagatc gatggcgtcg agcagctgaa
     1141 caacatcctc gttattggaa tgaccaatcg accagatttg attgatgagg ctctccttcg
     1201 acctggaaga ctggaagtta agatggagat aggcttacca gatgagaagg gccgactgca
     1261 gatccttcac atccacacag caaggatgcg agggcaccag ctactgtctg cggatgtgga
     1321 catcaaggaa ctggctgtgg agactaagaa tttcagtggc gccgagctgg agggcctggt
     1381 gcgagcagcg cagtccacag ccatgaacag acacataaag gccagcacaa aagtggaagt
     1441 agacatggag aaggcagaga gcctgcaggt gacaagaggg gacttcctcg cttccctgga
     1501 gaatgatatc aaaccggcat tcggcacaaa ccaagaggac tatgcaagtt acattatgaa
     1561 tggaatcatc aagtggggtg accccgtgac ccgagtcctc gacgacggag agctgctggt
     1621 ccagcaaact aaaaacagtg accgcacacc gctggttagc gtccttctgg aaggccctcc
     1681 tcacagcggg aagactgccc tcgctgcaaa gattgccgag gaatcaaact tccccttcat
     1741 caagatctgc tcccccgata agatgattgg gttctctgag acagccaagt gtcaggccat
     1801 gaagaagatc tttgatgatg cttacaaatc tcagctcagt tgtgtggtcg tggatgacat
     1861 tgagaggttg ctcgattacg tccccatcgg cccccggttc tccaacctgg tgttacaagc
     1921 tctgctcgtg ttactgaaaa aggcacctcc tcagggccgc aagcttctca tcatcgggac
     1981 caccagccgc aaagatgtcc tgcaggaaat ggagatgctc aacgccttca gcaccaccat
     2041 ccacgtgccc aacatcgcca caggagagca gctgctggaa gctctggagc ttttgggcaa
     2101 cttcaaagat aaggaacgca ccacaattgc tcagcaagtc aaagggaaga aagtctggat
     2161 aggaatcaag aagttactaa tgttgattga gatgtccctg cagatggacc ctgagtaccg
     2221 tgtgaggaaa ttcttggccc tcatgagaga agaaggcgct agtcccctgg actttgactg
     2281 aaaactggac tgtttacaac agtgaccaag gtgatgatgg cctgggatcg tcactcctca
     2341 tactcaagac actggactaa gtgaagtgtc cacctccctc tccacactca ctctgcatga
     2401 ctagtgcagt aaactgcctc gtgcttccga gatgtcctat gttcctggac tgctgtgctt
     2461 ccctaccatg ctggctaaga tgattcccgc ttacgtggga ggcacgccac gcctgtgcac
     2521 cctgcccaca cctcgctccc cgccacgccc acccttggaa aacacttgac ctacccgaag
     2581 gtaggcttct acacgtgacc aagagcaaag atagaggagg ttccaaggga ttcgcttagc
     2641 actccccgtg ttaacaacca tgtggcccac agaatgggtt attatctttg cctaaagaag
     2701 caggattttg aatatctacg atgctattct caatcatttt cagagatatt tccaagttgc
     2761 aatcaggaga gcttttctaa tcggaagtca gatcactcct gtggtggctg tgatatgccc
     2821 tgagtgtaat taatttgctc tgtggtaaga gatatgctaa tcattaccac ttggtagatg
     2881 ccgttaaaac cttatgaaag atacatatgg aaatctgtac atccctaaac agaaagaaaa
     2941 attaaaataa tttacaagcc tctcagttct aattagcgtc cagctctaag tgggtcattt
     3001 ccttgggatg acgttagagg cctattagtg ccatactctt tcctctgcct gatgacttat
     3061 aatttatctg ccctgtatct gaagtgatct gtaatgtcta aagcacataa ataaaacact
     3121 actctccagc agaacaagcc accccctagt cctctcctcg gtccttgaat atccattcca
     3181 gcgtactcaa cacacttctt cccaaaagag gaggagctca ccaagccctt tgcagtgtgg
     3241 tttgcctaat cagtgggtta gaagtaactt aattagatca tcattcctgg agacgagtct
     3301 tagggagcat gccactctcc ccgctgctgc ttaaaagaca gacagccatc agaagccacc
     3361 caggagactt gctgccacag gggcacctag gcgtgactct gacactgtcc ccacagtaga
     3421 aggcaaatca tcccctcacc ttgctgtccg tataccttgt ttggggtttt catccctggc
     3481 ttattttgtg gctttgaaag agtggattgt gttgtgaaac tctgattccc gtggctagta
     3541 ccgtggttcc ccgtccatca ccgtggtccc tgttgtctgc ggtcctgcct ttttcctccc
     3601 aacaaagttg tacgtggatt aatttatgtt tcttgttcaa cgaagttaat gtcttgtgtt
     3661 tttgtccaaa atcatattga aaaagataaa tattatattg tgtaatgcaa agcgaaggaa
     3721 tgcaataaag agtaaatata cttgaaatgt ta
//
