LOCUS       NM_001009399            1750 bp    mRNA    linear   ROD 07-JAN-2025
DEFINITION  Rattus norvegicus NAD(P) dependent 3-beta-hydroxysteroid
            dehydrogenase NSDHL (Nsdhl), mRNA.
ACCESSION   NM_001009399 XM_219730
VERSION     NM_001009399.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Moessinger,C., Kuerschner,L., Spandl,J., Shevchenko,A. and
            Thiele,C.
  TITLE     Human lysophosphatidylcholine acyltransferases 1 and 2 are located
            in lipid droplets where they catalyze the formation of
            phosphatidylcholine
  JOURNAL   J Biol Chem 286 (24), 21330-21339 (2011)
   PUBMED   21498505
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Jiang,F. and Herman,G.E.
  TITLE     Analysis of Nsdhl-deficient embryos reveals a role for Hedgehog
            signaling in early placental development
  JOURNAL   Hum Mol Genet 15 (22), 3293-3305 (2006)
   PUBMED   17028112
REFERENCE   3  (bases 1 to 1750)
  AUTHORS   Waterham,H.R.
  TITLE     Defects of cholesterol biosynthesis
  JOURNAL   FEBS Lett 580 (23), 5442-5449 (2006)
   PUBMED   16876788
  REMARK    Review article
REFERENCE   4  (bases 1 to 1750)
  AUTHORS   Caldas,H., Cunningham,D., Wang,X., Jiang,F., Humphries,L.,
            Kelley,R.I. and Herman,G.E.
  TITLE     Placental defects are associated with male lethality in bare
            patches and striated embryos deficient in the NAD(P)H Steroid
            Dehydrogenase-like (NSDHL) Enzyme
  JOURNAL   Mol Genet Metab 84 (1), 48-60 (2005)
   PUBMED   15639195
REFERENCE   5  (bases 1 to 1750)
  AUTHORS   Fujimoto,Y., Itabe,H., Sakai,J., Makita,M., Noda,J., Mori,M.,
            Higashi,Y., Kojima,S. and Takano,T.
  TITLE     Identification of major proteins in the lipid droplet-enriched
            fraction isolated from the human hepatocyte cell line HuH7
  JOURNAL   Biochim Biophys Acta 1644 (1), 47-59 (2004)
   PUBMED   14741744
REFERENCE   6  (bases 1 to 1750)
  AUTHORS   Herman,G.E.
  TITLE     Disorders of cholesterol biosynthesis: prototypic metabolic
            malformation syndromes
  JOURNAL   Hum Mol Genet 12 Spec No 1, R75-R88 (2003)
   PUBMED   12668600
  REMARK    Review article
REFERENCE   7  (bases 1 to 1750)
  AUTHORS   Liu,X.Y., Dangel,A.W., Kelley,R.I., Zhao,W., Denny,P.,
            Botcherby,M., Cattanach,B., Peters,J., Hunsicker,P.R., Mallon,A.M.,
            Strivens,M.A., Bate,R., Miller,W., Rhodes,M., Brown,S.D. and
            Herman,G.E.
  TITLE     The gene mutated in bare patches and striated mice encodes a novel
            3beta-hydroxysteroid dehydrogenase
  JOURNAL   Nat Genet 22 (2), 182-187 (1999)
   PUBMED   10369263
REFERENCE   8  (bases 1 to 1750)
  AUTHORS   Phillips,R.J. and Kaufman,M.H.
  TITLE     Bare-patches, a new sex-linked gene in the mouse, associated with a
            high production of XO females. II. Investigations into the nature
            and mechanism of the XO production
  JOURNAL   Genet Res 24 (1), 27-41 (1974)
   PUBMED   4435359
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC087626.1.
            
            On Jan 7, 2005 this sequence version replaced XM_219730.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087626.1, SRR8487226.4265.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq37"
     gene            1..1750
                     /gene="Nsdhl"
                     /note="NAD(P) dependent 3-beta-hydroxysteroid
                     dehydrogenase NSDHL"
                     /db_xref="GeneID:309262"
                     /db_xref="RGD:1359337"
     exon            1..74
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     exon            75..191
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1205
                     /gene="Nsdhl"
                     /EC_number="1.1.1.170"
                     /note="NAD(P) dependent steroid dehydrogenase-like"
                     /codon_start=1
                     /product="sterol-4-alpha-carboxylate 3-dehydrogenase,
                     decarboxylating"
                     /protein_id="NP_001009399.1"
                     /db_xref="GeneID:309262"
                     /db_xref="RGD:1359337"
                     /translation="MEQAVRSESKKGQVTGTDLINEVSKAKKCTVIGGSGFLGQHMVE
                     QLLSRGYAVNVFDVRQGFDNPRVQFFIGDLCNQQDLYPALKGVSTVFHCASPPSNSNN
                     KELFYRVNSTGTKTVIETCKEAGVQKLILTSSASVVFEGVDIKNGTEDLPYAMKPIDY
                     YTETKILQERAVLDANDPKKNFLTAAIRPHGIFGPRDPQLVPVLIDAARKGKMKFMIG
                     NGKNLVDFTFVENVVHGHILAAEHLSRDAGLGGKAFHITNDEPIPFWTFLSRILTGLN
                     YEAPKYHIPYRVAYYLAFLLSLLVMVLSPLIQIQTTFTPFRVALAGTFHYYSCEKAKK
                     LIGYRPLVTMDDAVERTVQSFHHLRKDK"
     exon            192..350
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     exon            351..497
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     exon            498..626
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     exon            627..769
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     exon            770..872
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1726
                     /gene="Nsdhl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctggtattca gctggagttc gctggttgcc ggttgcttgc aagctgaggt ctatcgtttg
       61 agtgtctaaa cccggaagaa gagttgatta caaacgagac catactttga gccataatgg
      121 aacaggctgt tcgtagtgaa tcaaagaaag gccaggtcac agggacagat ttgataaatg
      181 aagtttccaa agctaagaag tgcacagtga ttggaggctc tgggttcctg gggcagcaca
      241 tggtagagca gttgctatcg cgaggctatg ctgtcaatgt atttgatgtc cgccaaggct
      301 ttgataaccc tcgggtgcag ttcttcatag gtgacctgtg caaccaacag gacctgtacc
      361 cagctctcaa aggtgtaagc acagttttcc actgtgcgtc acccccgtca aacagtaaca
      421 acaaggagct cttttataga gtgaattcca ctggcaccaa gactgtcatt gaaacctgca
      481 aagaggctgg agttcagaaa ctcattttaa ccagctctgc cagtgttgtc tttgagggtg
      541 tcgatataaa aaatggaact gaagacctcc cttatgccat gaagcccatt gactactata
      601 cagagaccaa gatcttgcag gaaagagcag tactggatgc caacgaccct aagaagaatt
      661 ttttaactgc agccattcgt cctcatggca tatttggccc aagagatccc cagttggtcc
      721 cagtcttaat tgatgcagct agaaaaggca aaatgaagtt catgattgga aatgggaaga
      781 acctggtgga cttcaccttc gtggagaatg tagttcatgg acacatctta gcagctgagc
      841 acctctctcg agatgcaggt ctgggtggga aggcatttca catcaccaac gatgagccaa
      901 tccctttctg gacgttcctg tcccgcattc tgacaggcct caattatgag gcccccaagt
      961 accacatccc ctacagggtg gcctactatc ttgctttcct gctatctcta ctggtaatgg
     1021 tgctcagccc tctcatccaa atccagacaa cttttacacc atttcgggtg gcactggctg
     1081 gaacattcca ctattacagt tgtgagaaag ccaaaaagct cattgggtac cggccactgg
     1141 tcaccatgga tgatgctgtg gaaaggactg tgcagagttt ccaccacctg cggaaggaca
     1201 agtgaagctc cccagtgccc tcaccagtaa ctttctcctc tctgttctaa agaaccaata
     1261 tccctaagtg agtttcttct gagctgcatg ccccttgctg gttagatgac agcatacttg
     1321 actccctcta tgatattgga ggtgacaaca acaggagaca tttctcccag aatagcattg
     1381 gacttctcag gcagactcac agtctactgt tttggtcttt ctctctcctc cccctacttc
     1441 tgtctcctgg gttcattatc agaaagataa tactaaagtg aaagctttgt ttaaggtctt
     1501 aaaaattgaa gaaaatcaga aattgtaaag acagtaagac ttcagacata cattttataa
     1561 gatcacagta caatagttag aagtactgat gagtgtattc ccaatccctg gtccctaagg
     1621 ctaaatccac tgcttgttcc ttgctccctc gtatactctc aaggtctctt tcaaagatgg
     1681 ttgcagtgtt tgtctccatt gtttttccat aaagtatttc catttaaaaa aaaaaaaaaa
     1741 aaaaaaaaaa
//
