LOCUS       NM_021767               5592 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus neurexin 1 (Nrxn1), mRNA.
ACCESSION   NM_021767
VERSION     NM_021767.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Janz,P., Bainier,M., Marashli,S., Schoenenberger,P., Valencia,M.
            and Redondo,R.L.
  TITLE     Neurexin1alpha knockout rats display oscillatory abnormalities and
            sensory processing deficits back-translating key endophenotypes of
            psychiatric disorders
  JOURNAL   Transl Psychiatry 12 (1), 455 (2022)
   PUBMED   36307390
  REMARK    GeneRIF: Neurexin1alpha knockout rats display oscillatory
            abnormalities and sensory processing deficits back-translating key
            endophenotypes of psychiatric disorders.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Kang,Y., Xue,J., Zheng,J., Liang,J., Cai,C. and Wang,Y.
  TITLE     Upregulation of Hevin contributes to postoperative pain
            hypersensitivity by inducing neurexin1beta/neuroligin1-mediated
            synaptic targeting of GluA1-containing AMPA receptors in rat dorsal
            horn
  JOURNAL   Brain Res 1792, 148004 (2022)
   PUBMED   35820448
  REMARK    GeneRIF: Upregulation of Hevin contributes to postoperative pain
            hypersensitivity by inducing neurexin1beta/neuroligin1-mediated
            synaptic targeting of GluA1-containing AMPA receptors in rat dorsal
            horn.
REFERENCE   3  (bases 1 to 5592)
  AUTHORS   Zhang,S., Wu,D., Xu,Q., You,L., Zhu,J., Wang,J., Liu,Z., Yang,L.,
            Tong,M., Hong,Q. and Chi,X.
  TITLE     The protective effect and potential mechanism of NRXN1 on learning
            and memory in ADHD rat models
  JOURNAL   Exp Neurol 344, 113806 (2021)
   PUBMED   34228999
  REMARK    GeneRIF: The protective effect and potential mechanism of NRXN1 on
            learning and memory in ADHD rat models.
REFERENCE   4  (bases 1 to 5592)
  AUTHORS   Ribeiro,L.F., Verpoort,B., Nys,J., Vennekens,K.M., Wierda,K.D. and
            de Wit,J.
  TITLE     SorCS1-mediated sorting in dendrites maintains neurexin axonal
            surface polarization required for synaptic function
  JOURNAL   PLoS Biol 17 (10), e3000466 (2019)
   PUBMED   31658245
  REMARK    GeneRIF: SorCS1-mediated sorting in dendritic endosomes controls
            Nrxn axonal surface polarization required for proper synapse
            development and function.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5592)
  AUTHORS   Constance,W.D., Mukherjee,A., Fisher,Y.E., Pop,S., Blanc,E.,
            Toyama,Y. and Williams,D.W.
  TITLE     Neurexin and Neuroligin-based adhesion complexes drive axonal
            arborisation growth independent of synaptic activity
  JOURNAL   Elife 7, e31659 (2018)
   PUBMED   29504935
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5592)
  AUTHORS   Ichtchenko,K., Nguyen,T. and Sudhof,T.C.
  TITLE     Structures, alternative splicing, and neurexin binding of multiple
            neuroligins
  JOURNAL   J Biol Chem 271 (5), 2676-2682 (1996)
   PUBMED   8576240
REFERENCE   7  (bases 1 to 5592)
  AUTHORS   Ullrich,B., Ushkaryov,Y.A. and Sudhof,T.C.
  TITLE     Cartography of neurexins: more than 1000 isoforms generated by
            alternative splicing and expressed in distinct subsets of neurons
  JOURNAL   Neuron 14 (3), 497-507 (1995)
   PUBMED   7695896
REFERENCE   8  (bases 1 to 5592)
  AUTHORS   Ushkaryov,Y.A., Hata,Y., Ichtchenko,K., Moomaw,C., Afendis,S.,
            Slaughter,C.A. and Sudhof,T.C.
  TITLE     Conserved domain structure of beta-neurexins. Unusual cleaved
            signal sequences in receptor-like neuronal cell-surface proteins
  JOURNAL   J Biol Chem 269 (16), 11987-11992 (1994)
   PUBMED   8163501
REFERENCE   9  (bases 1 to 5592)
  AUTHORS   Perin,M.S.
  TITLE     The COOH terminus of synaptotagmin mediates interaction with the
            neurexins
  JOURNAL   J Biol Chem 269 (11), 8576-8581 (1994)
   PUBMED   8132583
REFERENCE   10 (bases 1 to 5592)
  AUTHORS   Ushkaryov,Y.A., Petrenko,A.G., Geppert,M. and Sudhof,T.C.
  TITLE     Neurexins: synaptic cell surface proteins related to the
            alpha-latrotoxin receptor and laminin
  JOURNAL   Science 257 (5066), 50-56 (1992)
   PUBMED   1621094
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            [WARNING] On Oct 22, 2024 this sequence was replaced by
            NM_021767.4.
            
            On Nov 27, 2020 this sequence version replaced NM_021767.2.
            
            Summary: This gene encodes a single-pass type I membrane protein
            that belongs to the neurexin family. Neurexins are synaptic
            transmembrane receptors that bind endogenous ligands that include
            neuroligins, dystroglycan, and neurexophilins. Neurexin complexes
            are required for efficient neurotransmission and are involved in
            synaptogenesis. In vertebrates, alternate promoter usage results in
            multiple isoform classes, of which the alpha and beta classes are
            the best characterized. In humans, allelic variants in this gene
            are associated with Pitt-Hopkins-like syndrome-2, while deletions
            have been associated with autism and schizophrenia. Mouse knockouts
            display decreased spontaneous and evoked vesicle release resulting
            in impaired synaptic transmission. In addition, knockout mice show
            altered social approach, reduced social investigation, reduced
            locomotor activity, and in males, increased aggression. [provided
            by RefSeq, Nov 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M96374.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1626              JAXUCZ010000006.1  8934868-8936493
            1627-1644           JAXUCZ010000006.1  9040484-9040501
            1645-1674           JAXUCZ010000006.1  9042642-9042671
            1675-1686           JAXUCZ010000006.1  9043426-9043437
            1687-1988           JAXUCZ010000006.1  9384416-9384717
            1989-2012           JAXUCZ010000006.1  9386835-9386858
            2013-2174           JAXUCZ010000006.1  9387864-9388025
            2175-2613           JAXUCZ010000006.1  9457231-9457669
            2614-2997           JAXUCZ010000006.1  9473049-9473432
            2998-3201           JAXUCZ010000006.1  9480714-9480917
            3202-3228           JAXUCZ010000006.1  9483376-9483402
            3229-3351           JAXUCZ010000006.1  9505900-9506022
            3352-3733           JAXUCZ010000006.1  9514063-9514444
            3734-3924           JAXUCZ010000006.1  9515572-9515762
            3925-4098           JAXUCZ010000006.1  9538277-9538450
            4099-4218           JAXUCZ010000006.1  9545583-9545702
            4219-4400           JAXUCZ010000006.1  9753984-9754165
            4401-4572           JAXUCZ010000006.1  9910596-9910767
            4573-4662           JAXUCZ010000006.1  9941381-9941470
            4663-4982           JAXUCZ010000006.1  9942806-9943125
            4983-5070           JAXUCZ010000006.1  10053189-10053276
            5071-5592           JAXUCZ010000006.1  10074043-10074564
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q11-q12"
     gene            1..5592
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /note="neurexin 1"
                     /db_xref="GeneID:60391"
                     /db_xref="RGD:628659"
     exon            1..1626
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             855..5378
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /note="neurexin-1-beta; neurexin-1-alpha; 60S ribosomal
                     protein L10a pseudogene; neurexin I-beta; neurexin
                     I-alpha; non-processed neurexin I-alpha"
                     /codon_start=1
                     /product="neurexin-1 precursor"
                     /protein_id="NP_068535.3"
                     /db_xref="GeneID:60391"
                     /db_xref="RGD:628659"
                     /translation="MGTALVQHGGCCLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFP
                     KWNACCESEMSFQLKTRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPAT
                     LLADTPVNDGAWHSVRIRRQFRNTTLYIDRAEAKWVEVKSKRRDMTVFSGLFVGGLPP
                     ELRAAALKLTLASVREREPFKGWIRDVRVNSSQALPVDGSEVKLDEEPPNSGGGSPCE
                     AGDEGDGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKS
                     KGKEEYIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLA
                     LKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVD
                     GILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRL
                     ELSRLAKQGDPKMKIHGVVAFKCENVATLDPITFETPESFISLPKWNAKKTGSISFDF
                     RTTEPNGLILFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGTIKIKAL
                     QKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAG
                     LVFPTEVWTALLNYGYVGCIRDLFIDGQSKDIRQMAEIQSTAGVKPSCSRETAKPCLS
                     NPCKNNGMCRDGWNRYVCDCSGTGYLGRSCEREATVLSYDGSMFMKIQLPVVMHTEAE
                     DVSLRFRSQRAYGILMATTSRDSADTLRLELDAGRVKLTVNLDCIRINCNSSKGPETL
                     FAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHTRLEFHNIETGIITERR
                     YLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFRNIIADPVTFKTKSS
                     YVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGA
                     NLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGG
                     VAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQ
                     EDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWPPNDRPS
                     TRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESN
                     AIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGNNDNERLAIARQRIPYRLGRVV
                     DEWLLDKGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAI
                     VGNVRLVGEVPSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTT
                     DDILVASAECPSDDEDIDPCEPSSGGLANPTRVGGREPYPGSAEVIRESSSTTGMVVG
                     IVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSAQSNGAVVKEKQPSSAKSA
                     NKNKKNKDKEYYV"
     exon            1627..1644
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1674
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1686
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1988
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2012
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2174
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2613
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2997
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3201
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3202..3228
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3229..3351
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3733
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3734..3924
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..4098
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4218
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            4219..4400
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            4401..4572
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4662
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            4663..4982
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            4983..5070
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
     exon            5071..5592
                     /gene="Nrxn1"
                     /gene_synonym="Nrxn1b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagatttctg ggttcatata aaacaacaaa atacaatcaa aatgcatcac caacatacac
       61 ccaattgaca tgctggaatc ttagggtggt cttgcagaga caaagacaag agaagagctg
      121 ttgggattga tttcaccatc taacagcttc tggctgtctc acaccagggt ttgagaccta
      181 ccgacaggac tcgtagggca tacacaggaa atttggcctt ggcatcagtg gtgctggaag
      241 cctgtgatat gacagtaggc ctctgaagct attctggtgt atcactctgc cccttctcct
      301 tctgtgtttg gagccaacat ctggaggagc ctcgaggcct gccaagcagc cgaaggtcct
      361 ggcttagttc tggcttcgtg ttcttctctg agaaaagtgt gttctttctg attctgctct
      421 cctattgctg gcaccacgcc ggtggtagtt gaaggtgttg gttctgcaag agtgaagtac
      481 ctgatggtgc cacagatgcc cttacttaac atcatcgttt gtaaatgaat cagtgatctc
      541 tcttgagcct cggtggcctt ctttttcaga acgttgcctc caaagtgtat cctccctttg
      601 gcctggaagt tctgtggcta tcccatacta ctggggtctc tgctttccag ttggaagggg
      661 actactggga gagaggaagg ggctgctgga agcgaagaag aaggaaaaaa gagctgaagg
      721 agggtgcgcc cctcccactg ccgcctcccc catccctccc actcctctgg ggacccccat
      781 tctccgtgac gttgtccagg accacttcga ccctgtcgcc cccagctccc ccccgccgca
      841 ccccagcccc gagcatgggg acggcgctgg tccagcatgg gggctgctgt ctcctctgcc
      901 tgtcgctgct gctgttgggc tgctgggcag agctgggcag tgggctggag ttcccaggcg
      961 ctgagggcca gtggacgcgc ttccccaagt ggaacgcgtg ctgcgagagt gagatgagct
     1021 tccagctgaa gacacgcagc gcccgcggcc tcgtgctcta cttcgacgac gagggcttct
     1081 gcgacttcct cgagctcatc ctgacacgcg gcggccgctt gcagctcagc ttctccatct
     1141 tctgcgccga gcccgccacg ctgttggccg acacgccagt caacgacggc gcctggcaca
     1201 gcgtgcgcat ccgccgccag ttccgcaaca ccactctcta catcgaccgc gctgaggcca
     1261 agtgggtgga ggtcaagtcc aagcgcaggg acatgacggt gttcagtggg ctgttcgtgg
     1321 gcggcctgcc gcctgaactg cgagccgctg cgctcaagct caccctggcc tcggtgcgtg
     1381 agcgggagcc cttcaagggc tggatccgtg atgtcagggt caactcgtcc caggcgctgc
     1441 cggtggacgg cagcgaggtc aagctggacg aagagccgcc caatagcggg ggcgggagcc
     1501 cgtgcgaggc gggcgatgag ggcgacggcg gcgtgtgcct caacgggggc gtgtgctccg
     1561 tggtcgacga ccaggccgtg tgtgactgct cgaggaccgg tttccgtggc aaagactgca
     1621 gccaagaaga caacaatgta gaaggtctgg cgcacctgat gatgggcgac caaggtaaaa
     1681 gtaaaggaaa agaagagtat attgccacat tcaagggatc tgagtacttc tgctacgact
     1741 tatcccaaaa ccccattcaa agcagcagcg acgaaataac tctgtccttt aaaactcttc
     1801 agaggaatgg actgatgctt cacacaggga aatcggctga ttatgtcaac cttgccttga
     1861 aaaatggagc tgtctctctg gtcattaatt tgggatcagg ggcctttgaa gcgctagtgg
     1921 agcccgtgaa tggaaagttt aatgataatg cctggcatga tgtgaaagtc accaggaatc
     1981 tgcgtcagca ctcaggcatt ggacacgcta tggtgacaat atcagtggat gggattctta
     2041 ccacaacggg ctacacgcaa gaagattaca ccatgctggg gtctgatgac tttttctatg
     2101 ttggaggcag ccccagcaca gccgaccttc cagggtcacc agtcagtaac aactttatgg
     2161 gctgtctcaa agaggtcgta tataaaaata atgatgtaag actggagtta tcgcgacttg
     2221 ccaagcaagg agatcctaag atgaagatcc atggtgtggt ggcttttaag tgtgaaaatg
     2281 tggcaacctt agacccaatc acttttgaga ccccagaatc tttcatttct ttgcctaagt
     2341 ggaatgcaaa gaaaacaggc tcaatatcat ttgatttccg tacaacggag ccaaatggcc
     2401 tcatcttgtt tagccatggc aagccaagac atcagaagga tgccaagcac ccgcagatga
     2461 ttaaggttga cttttttgct attgaaatgc tggatggcca cttgtacctc ctcttagaca
     2521 tgggatcagg tactataaaa atcaaagccc ttcagaagaa agtcaatgat ggagaatggt
     2581 atcacgtgga tttccagcga gatggacggt caggtaccat ttctgtcaac acacttcgta
     2641 ctccctacac agctcccggt gagagtgaga tcctagacct cgatgatgag ttgtatctgg
     2701 gtggcttgcc agaaaataag gctggcctgg tcttccccac tgaggtgtgg actgctctgc
     2761 tcaactatgg ctacgtaggc tgcatccggg atctattcat tgatggccag agcaaagata
     2821 tccggcagat ggcggaaatt cagagtactg ctggagtgaa gccatcctgc tcaagggaga
     2881 ctgcaaaacc atgccttagc aatccttgca aaaacaatgg catgtgcagg gatggctgga
     2941 acagatacgt ctgtgattgc tctggaacag gctatctcgg caggtcctgt gagagagagg
     3001 caacggtttt gagctacgat gggagtatgt ttatgaaaat tcagctccct gtggtgatgc
     3061 acactgaggc cgaggacgtt tccttacggt tcagatccca gcgtgcgtac ggcattctga
     3121 tggcgaccac ttctagagac tccgcagata cccttcgact ggagctagat gcagggcgcg
     3181 tgaaactcac ggtcaatcta gattgtatca ggattaactg taattccagc aaaggtcctg
     3241 agaccctttt tgccggctat aacctcaatg ataatgaatg gcacacagta cgtgtggttc
     3301 ggcgaggaaa aagtttaaag ctaaccgtgg atgatcaaca agccatgaca ggccaaatgg
     3361 caggtgatca tacaaggctg gagtttcata acatagagac aggcatcatc acagaacgac
     3421 gctatctttc ttctgtcccc tccaacttca ttgggcacct gcaaagcctc acatttaatg
     3481 gaatggcata cattgacttg tgcaaaaatg gtgatataga ttattgtgaa ctcaatgcca
     3541 ggtttggatt caggaacatc atcgcggatc ctgtcacctt caagaccaaa tcaagctatg
     3601 ttgccttagc tacattgcaa gcctacactt ctatgcatct atttttccaa ttcaagacaa
     3661 catccctaga tggactaatt ctgtataaca gtggggatgg aaatgacttt attgtggttg
     3721 aattagttaa agggtactta cattatgtgt ttgacttggg aaatggtgct aacctcatca
     3781 aagggagctc aaacaaacca ctcaacgaca atcagtggca caatgtgatg atttcaaggg
     3841 acaccagcaa tctccacaca gtaaagatcg acacaaaaat cacaacacaa atcactgcgg
     3901 gagccagaaa cttagacctc aagagtgact tgtatattgg aggagtggct aaagaaacat
     3961 acaaatcgtt gccgaaactg gtccatgcta aagaaggctt tcaaggttgc ctggcatctg
     4021 tggacttgaa tggcaggctt ccagacctca tatctgatgc tcttttctgc aatgggcaaa
     4081 ttgagagagg atgcgaaggg cccagcacaa cctgtcaaga ggactcatgt tcaaatcaag
     4141 gcgtgtgctt gcagcaatgg gatggcttta gctgtgactg tagcatgact tccttcagtg
     4201 gcccgctctg caatgaccct gggacgacgt atatctttag caaaggtggt ggacaaatta
     4261 catataagtg gcctcctaat gaccgaccca gtacacgagc agacaggctg gccatcgggt
     4321 ttagcactgt tcagaaggaa gcagtgttgg tgcgtgtgga cagttcctca ggcctgggtg
     4381 actaccttga gctgcacata caccaaggaa aaattggagt taaatttaat gttgggacag
     4441 atgacatcgc cattgaggag tccaatgcaa tcattaatga tggaaaatac catgtagtgc
     4501 gtttcacgag gagtggtggc aatgccacgt tacaggtgga cagctggcca gtcatcgaac
     4561 gataccctgc aggaaacaat gataacgagc gcctggcgat tgctagacag cgaattccat
     4621 atcgacttgg tcgagtagtt gatgaatggc tactcgacaa agggcgtcag ctcacaatct
     4681 tcaatagcca agcaaccata ataattggcg ggaaagagca gggccagccc ttccagggcc
     4741 agctctctgg gctttactac aatggcttga aagttctgaa tatggcggca gagaacgatg
     4801 ccaacatcgc catagtggga aatgtgaggc tggtcggtga agtgccttcc tctatgacaa
     4861 ctgagtcgac agccactgcc atgcagtccg agatgtccac ctcaatcatg gagaccacca
     4921 caaccctggc taccagcaca gctcgacgag gaaagccccc cacaaaggag cctatcagcc
     4981 agaccacgga tgatatcctt gtggcctcgg cagagtgtcc cagcgacgat gaggacattg
     5041 acccctgtga gccgagctca ggtgggttag ccaaccccac ccgagtgggc ggccgcgaac
     5101 catacccagg ctcggcagag gtgatccggg agtctagcag taccactggc atggtggtgg
     5161 ggattgtcgc agcagcagct ctgtgcatcc tcatcctcct ctatgccatg tacaagtaca
     5221 gaaaccggga tgaaggctca tatcacgtgg atgagagtcg aaactacatc agtaactcag
     5281 cacagtccaa tggggctgtg gtcaaggaga agcagcccag cagtgcgaaa agcgccaaca
     5341 aaaacaagaa gaacaaggat aaggagtatt atgtctgatc tcaagattcg aaaaggacac
     5401 ttgtatagaa atagtcttca ttttatctga gacataatat aaacttattt actttccttt
     5461 ttatgaagca catacaaaag aagacaggga atgcgatcag gagggaaaac tctttttaaa
     5521 aaaaaacaaa caagtatgtc atgctcttgt ttctcaaaaa aaaaacatac acaaaaaaaa
     5581 ggaaagagag aa
//
