LOCUS       NM_001025744            1554 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus NPR2-like, GATOR1 complex subunit (Nprl2), mRNA.
ACCESSION   NM_001025744 XM_343475
VERSION     NM_001025744.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Wolfson,R.L., Chantranupong,L., Wyant,G.A., Gu,X., Orozco,J.M.,
            Shen,K., Condon,K.J., Petri,S., Kedir,J., Scaria,S.M.,
            Abu-Remaileh,M., Frankel,W.N. and Sabatini,D.M.
  TITLE     KICSTOR recruits GATOR1 to the lysosome and is necessary for
            nutrients to regulate mTORC1
  JOURNAL   Nature 543 (7645), 438-442 (2017)
   PUBMED   28199306
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Bar-Peled,L. and Sabatini,D.M.
  TITLE     Regulation of mTORC1 by amino acids
  JOURNAL   Trends Cell Biol 24 (7), 400-406 (2014)
   PUBMED   24698685
  REMARK    Review article
REFERENCE   3  (bases 1 to 1554)
  AUTHORS   Bar-Peled,L., Chantranupong,L., Cherniack,A.D., Chen,W.W.,
            Ottina,K.A., Grabiner,B.C., Spear,E.D., Carter,S.L., Meyerson,M.
            and Sabatini,D.M.
  TITLE     A Tumor suppressor complex with GAP activity for the Rag GTPases
            that signal amino acid sufficiency to mTORC1
  JOURNAL   Science 340 (6136), 1100-1106 (2013)
   PUBMED   23723238
REFERENCE   4  (bases 1 to 1554)
  AUTHORS   Kurata,A., Katayama,R., Watanabe,T., Tsuruo,T. and Fujita,N.
  TITLE     TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1
            tyrosine phosphorylation and its downstream signaling
  JOURNAL   Cancer Sci 99 (9), 1827-1834 (2008)
   PUBMED   18616680
REFERENCE   5  (bases 1 to 1554)
  AUTHORS   Lee,N.H., Weinstock,K.G., Kirkness,E.F., Earle-Hughes,J.A.,
            Fuldner,R.A., Marmaros,S., Glodek,A., Gocayne,J.D., Adams,M.D.,
            Kerlavage,A.R. et al.
  TITLE     Comparative expressed-sequence-tag analysis of differential gene
            expression profiles in PC-12 cells before and after nerve growth
            factor treatment
  JOURNAL   Proc Natl Acad Sci U S A 92 (18), 8303-8307 (1995)
   PUBMED   7667285
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CV077924.1, DV714395.1, AA684618.1 and BI285715.1.
            
            On Oct 19, 2007 this sequence version replaced NM_001025744.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-647               CV077924.1         1-647
            648-1278            DV714395.1         56-686
            1279-1425           AA684618.1         1-147               c
            1426-1554           BI285715.1         1-129               c
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q32"
     gene            1..1554
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /note="NPR2-like, GATOR1 complex subunit"
                     /db_xref="GeneID:363138"
                     /db_xref="RGD:1308927"
     exon            1..213
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..1278
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /note="tumor suppressor candidate 4; GATOR complex protein
                     NPRL2"
                     /codon_start=1
                     /product="GATOR1 complex protein NPRL2"
                     /protein_id="NP_001020915.2"
                     /db_xref="GeneID:363138"
                     /db_xref="RGD:1308927"
                     /translation="MGSSCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYI
                     ITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIV
                     KKLAGYLTTLELESSFVSTEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVI
                     EQRPDPPVAQEYDVPVFTKDKEDFFSSQWDLTTQQILPYIDGFRHVQKISAEADVELN
                     LVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRA
                     SLRDVFQLYCSLSPGTTVRDLIGRHPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVS
                     REERSHPARLYTGCHSYDEICCKTGMSYHELDERLENDPNIIICWK"
     exon            214..305
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            306..474
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            475..583
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            584..720
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            721..818
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            819..855
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            856..949
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1067
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1210
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1537
                     /gene="Nprl2"
                     /gene_synonym="Tusc4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acggctcttt tggtttgagc tctcaaacct gtgtgaccta gccaggcctc tttctctttt
       61 tgacaggtat tctaaggggt ggaggcgacc accacacgaa aagcaggggt tacagacttg
      121 ggtccggaca gcgccatggg cagcagctgc cgcatcgaat gcatattctt cagcgagttc
      181 caccccacgc tgggacccaa aattacctat caggtccctg aggatttcat ctcccgagag
      241 ctgtttgaca ctgtccaagt atacatcatc accaagcccg agttacagaa caagcttatc
      301 actgtcacgg ccatggagaa gaagctgatt ggctgccccg tgtgcatcga acacaagaag
      361 tacagccgca acgcactcct cttcaacctg ggcttcgtgt gtgatgctca ggctaagact
      421 tgtgccctgg aacccattgt taagaagctg gccggctact taaccacact ggagctcgaa
      481 agtagctttg tatccaccga ggagagcaag cagaagctag tgcccatcat gactatctta
      541 ctggaagagc taaatgcctc tggccggtgc actctaccca tcgatgaatc caacaccatc
      601 cacttgaagg tgattgagca gcggcctgac cctcccgttg cgcaggaata tgatgtgcct
      661 gtctttacaa aggacaaaga ggacttcttc agctcccagt gggacctcac cacacagcag
      721 atcctcccct acatcgatgg atttcgtcat gtccaaaaga tctccgctga ggcagacgtg
      781 gagctcaacc tggtgcgcat tgccattcag aacctgctgt actatggtgt tgtgacactg
      841 gtatccattc tccagtactc caatgtgtat tgcccaacgc caaaagtcca ggacctggtg
      901 gatgacaagt ccctgcagga ggcatgtcta tcctacgtca ccaaacaagg acacaaaaga
      961 gccagtctcc gagatgtatt ccagctgtac tgcagcctga gccctggtac cactgtgcga
     1021 gacctcatcg gccgccaccc ccagcagtta caacatgttg atgaaaggaa gctgatccag
     1081 tttgggctta tgaagaacct catccggaga ctacagaaat atccagtccg agtgtctcgg
     1141 gaggaacgaa gccaccctgc ccggctctac acaggctgtc acagctatga tgagatctgc
     1201 tgcaagacag gcatgagcta ccatgagctg gacgagcgac tagaaaatga cccaaacatc
     1261 atcatctgct ggaagtgagg ctggctggct ggagcatatt actgtggtta cttgccccta
     1321 ctaagccctc attggtccat gggagctaga caagtagtct agtacatgtg gtctggaggg
     1381 gtgagcctct gttttgtaaa taaagtcatg tgtttcatcc tgtggaatat aaaggccagt
     1441 gaatgtgaaa gctatgtcaa ccgactgggt tgactggagc gccatctagt ggacagaaat
     1501 gtcaataaac aggccaagag gctttgtttt atttttaaaa aaaaaaaaaa aaaa
//
