LOCUS       NM_130828               1631 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus NPHS2 stomatin family member, podocin (Nphs2),
            mRNA.
ACCESSION   NM_130828
VERSION     NM_130828.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Baijnath,S., Murugesan,S., Mackraj,I., Gathiram,P. and Moodley,J.
  TITLE     The effects of sildenafil citrate on urinary podocin and nephrin
            mRNA expression in an L-NAME model of pre-eclampsia
  JOURNAL   Mol Cell Biochem 427 (1-2), 59-67 (2017)
   PUBMED   27995418
  REMARK    GeneRIF: The effects of sildenafil citrate on urinary podocin and
            nephrin mRNA expression in an L-NAME model of pre-eclampsia.
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Fukuda,H., Hidaka,T., Takagi-Akiba,M., Ichimura,K., Oliva
            Trejo,J.A., Sasaki,Y., Wang,J., Sakai,T., Asanuma,K. and Tomino,Y.
  TITLE     Podocin is translocated to cytoplasm in puromycin aminonucleoside
            nephrosis rats and in poor-prognosis patients with IgA nephropathy
  JOURNAL   Cell Tissue Res 360 (2), 391-400 (2015)
   PUBMED   25676004
  REMARK    GeneRIF: translocation of podocin by endocytosis could be a key
            traffic event of critical podocyte injury and that the podocin gap
            could indicate the prognosis of IgA nephropathy.
REFERENCE   3  (bases 1 to 1631)
  AUTHORS   Enatsu,N., Miyake,H., Chiba,K. and Fujisawa,M.
  TITLE     Candesartan Mediated Amelioration of Cisplatin-Induced Testicular
            Damage Is Associated with Alterations in Expression Patterns of
            Nephrin and Podocin
  JOURNAL   Biomed Res Int 2015, 273784 (2015)
   PUBMED   26539476
  REMARK    GeneRIF: These findings suggest that candesartan can prevent
            cisplatin-induced testicular damage by regulating expression
            patterns of the nephrin-podocin complex in the testes.
REFERENCE   4  (bases 1 to 1631)
  AUTHORS   Pan,Q.R., Ren,Y.L., Zhu,J.J., Hu,Y.J., Zheng,J.S., Fan,H., Xu,Y.,
            Wang,G. and Liu,W.X.
  TITLE     Resveratrol increases nephrin and podocin expression and alleviates
            renal damage in rats fed a high-fat diet
  JOURNAL   Nutrients 6 (7), 2619-2631 (2014)
   PUBMED   25025298
  REMARK    GeneRIF: Resveratrol alleviated dyslipidemia and renal damage
            induced by high-fat diet also reduced TNF-alpha and MCP-1 levels,
            and reduced expressions of nephrin and podocin.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1631)
  AUTHORS   Roshanravan,H. and Dryer,S.E.
  TITLE     ATP acting through P2Y receptors causes activation of podocyte
            TRPC6 channels: role of podocin and reactive oxygen species
  JOURNAL   Am J Physiol Renal Physiol 306 (9), F1088-F1097 (2014)
   PUBMED   24553432
  REMARK    GeneRIF: ATP evokes activation of TRPC6 channels in podocytes
            through P2Y receptors, podocin, and generation of reactive oxygen
            species.
REFERENCE   6  (bases 1 to 1631)
  AUTHORS   Nakatsue,T., Koike,H., Han,G.D., Suzuki,K., Miyauchi,N., Yuan,H.,
            Salant,D.J., Gejyo,F., Shimizu,F. and Kawachi,H.
  TITLE     Nephrin and podocin dissociate at the onset of proteinuria in
            experimental membranous nephropathy
  JOURNAL   Kidney Int 67 (6), 2239-2253 (2005)
   PUBMED   15882266
REFERENCE   7  (bases 1 to 1631)
  AUTHORS   Li,H., Lemay,S., Aoudjit,L., Kawachi,H. and Takano,T.
  TITLE     SRC-family kinase Fyn phosphorylates the cytoplasmic domain of
            nephrin and modulates its interaction with podocin
  JOURNAL   J Am Soc Nephrol 15 (12), 3006-3015 (2004)
   PUBMED   15579503
  REMARK    GeneRIF: Fyn phosphorylates the cytoplasmic domain of nephrin on
            tyrosine, leading to enhanced association with podocin and
            downstream signaling of nephrin.
REFERENCE   8  (bases 1 to 1631)
  AUTHORS   Kawachi,H., Koike,H., Kurihara,H., Sakai,T. and Shimizu,F.
  TITLE     Cloning of rat homologue of podocin: expression in proteinuric
            states and in developing glomeruli
  JOURNAL   J Am Soc Nephrol 14 (1), 46-56 (2003)
   PUBMED   12506137
  REMARK    GeneRIF: podocin is one of critical components of slit diaphragm
            for maintaining barrier function of glomerular capillary wall
REFERENCE   9  (bases 1 to 1631)
  AUTHORS   Roselli,S., Gribouval,O., Boute,N., Sich,M., Benessy,F., Attie,T.,
            Gubler,M.C. and Antignac,C.
  TITLE     Podocin localizes in the kidney to the slit diaphragm area
  JOURNAL   Am J Pathol 160 (1), 131-139 (2002)
   PUBMED   11786407
REFERENCE   10 (bases 1 to 1631)
  AUTHORS   Schwarz,K., Simons,M., Reiser,J., Saleem,M.A., Faul,C., Kriz,W.,
            Shaw,A.S., Holzman,L.B. and Mundel,P.
  TITLE     Podocin, a raft-associated component of the glomerular slit
            diaphragm, interacts with CD2AP and nephrin
  JOURNAL   J Clin Invest 108 (11), 1621-1629 (2001)
   PUBMED   11733557
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Nov 27, 2020 this sequence version replaced NM_130828.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY039651.1, BC098649.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-335               JAXUCZ010000013.1  70999186-70999520
            336-439             JAXUCZ010000013.1  71001441-71001544
            440-512             JAXUCZ010000013.1  71002742-71002814
            513-595             JAXUCZ010000013.1  71004767-71004849
            596-799             JAXUCZ010000013.1  71006329-71006532
            800-855             JAXUCZ010000013.1  71008665-71008720
            856-934             JAXUCZ010000013.1  71009924-71010002
            935-1631            JAXUCZ010000013.1  71010876-71011572
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q22"
     gene            1..1631
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /note="NPHS2 stomatin family member, podocin"
                     /db_xref="GeneID:170672"
                     /db_xref="RGD:620461"
     exon            1..335
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1213
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /note="nephrosis 2 homolog, podocin; NPHS2, podocin;
                     nephrosis 2, idiopathic, steroid-resistant"
                     /codon_start=1
                     /product="podocin"
                     /protein_id="NP_570841.3"
                     /db_xref="GeneID:170672"
                     /db_xref="RGD:620461"
                     /translation="MDSRARSSSRKTHGRGSRSSSRDDKKSKAGRGNRGRARPDAGAE
                     RQSAGRTGTREEHRAPAATVVNVDEVRSPGEEGTEVVALLESERPEEGIKPSGLGACE
                     WLLVLSSLIFIIVTFPFSIWFCIKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLD
                     TYHKVDLRLQTLEIPFHEVVTKDMFIMEIDAVCYYRMENASLLLSSLAHVSKAIQFLV
                     QTTMKRLLAHRSLTEILLERKSIAQDVKVALDSVTCVWGIKVERTEIKDVRLPAGLQH
                     SLAVEAEAQRQAKVRVIAAEGEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTDK
                     PSTVVLPLPFDMLNLLSSPSNRAQGSINYPSSPKPVEPLNPKRKDSPML"
     exon            336..439
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     exon            440..512
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     exon            513..595
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     exon            596..799
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     exon            800..855
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     exon            856..934
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1631
                     /gene="Nphs2"
                     /gene_synonym="podocin"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agaaaattgg ggctgcgact ctgccagcat ctggctttga ggggcgtgcc cgccgcgtag
       61 aatggacagc agggcgcgga gctcttccag aaagacccac gggagaggta gcaggtcctc
      121 ttctagggat gacaagaagt caaaggccgg gaggggcaac agaggccgcg cgcgcccgga
      181 tgctggggca gagcggcaga gcgccgggcg gacggggacc cgggaggagc accgagctcc
      241 agcagccacg gtagtgaatg tggacgaggt tcgaagcccg ggtgaggagg gtacggaagt
      301 ggtggccctg ctggagagcg agcgaccaga ggaagggatc aagccctctg gattaggggc
      361 ctgcgagtgg cttcttgtcc tctcctccct gatcttcatc atcgtaacgt ttcccttttc
      421 catctggttc tgcataaagg ttgttcaaga atacgaaaga gtaattatat tccgactggg
      481 acatctgctt cctggaagag ccaaaggacc tggcctgttc ttttttctac cctgcctgga
      541 cacctatcac aaggttgacc tccgtctcca gaccttggaa atacctttcc atgaggtggt
      601 aaccaaagat atgttcataa tggagataga cgctgtctgc tactaccgca tggaaaatgc
      661 ctcccttctt ctaagcagtc tagctcatgt gtccaaagcc atccagttcc tggtgcaaac
      721 caccatgaag cgcctcttgg cacatcgatc cctcactgaa attctcctgg aaaggaagag
      781 cattgcccaa gatgtaaagg ttgccttgga ctcagtgacc tgtgtttggg gcatcaaagt
      841 ggagagaact gaaattaagg atgtacggct gccagctggg cttcagcact ctctggctgt
      901 ggaagctgag gcacaaagac aggccaaagt gcgggtgatt gctgccgaag gggaaaaagc
      961 tgcctctgag tccctgagga tggcggctga gattctgtcg ggcaccccag ctgctgtcca
     1021 gctccggtac ctgcacactc ttcagtcctt gtccacagac aagccgtcca ccgtggtttt
     1081 gcctttaccc tttgacatgc tgaaccttct ctcctctccc agcaacagag cacaaggaag
     1141 catcaactac ccaagttctc ccaaacctgt tgaaccacta aatcccaaaa ggaaggactc
     1201 tcctatgcta taggggcaag tggacaagag taatgggaat acaccatata aagccgtatc
     1261 cctgagcgag gcattcggtc cccacgccca ggcccaccct gcccttgttg tttgcctttt
     1321 gagtgtatca tgtcacaaga tggacacacg catgagaaca cagtgaaatg gcagagaaga
     1381 catccagcca cacaagtggg tcgtctcatc attcattaca ggaaagaaag agatttagaa
     1441 ttttgggttg aggggctgga gagatggctc agtggttatg aacactgact gctcttccag
     1501 aggtcctgag ttcaaatccc agcaaccaca tggtggctca caaccatctg taatgggatc
     1561 cgatgccctc ttctggtgtg taagacagtg acagtgtact catcatatat aagatgaata
     1621 aataaaacct t
//
