LOCUS       NM_022586                452 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus neuropeptide FF-amide peptide precursor (Npff),
            mRNA.
ACCESSION   NM_022586
VERSION     NM_022586.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Wojciechowski,P., Andrzejewski,K. and Kaczynska,K.
  TITLE     Intracerebroventricular Neuropeptide FF Diminishes the Number of
            Apneas and Cardiovascular Effects Produced by Opioid Receptors'
            Activation
  JOURNAL   Int J Mol Sci 21 (23), 8931 (2020)
   PUBMED   33255594
  REMARK    GeneRIF: Intracerebroventricular Neuropeptide FF Diminishes the
            Number of Apneas and Cardiovascular Effects Produced by Opioid
            Receptors' Activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  AUTHORS   Lin,Y.T., Yu,Y.L., Hong,W.C., Yeh,T.S., Chen,T.C. and Chen,J.C.
  TITLE     NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in
            Rodents
  JOURNAL   Int J Mol Sci 18 (8), 1810 (2017)
   PUBMED   28825666
  REMARK    GeneRIF: we demonstrate, for the first time, that NPFFR2 positively
            modulates the HPA axis via hypothalamic corticotropin-releasing
            factor (CRF). Our finding that NPFFR2 regulates stress-dependent
            neural pathways advances our understanding of neuroendocrine
            regulation and opens new avenues for probing the functions of NPFF.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 452)
  AUTHORS   Grasso,G., Mielczarek,P., Niedziolka,M. and Silberring,J.
  TITLE     Metabolism of cryptic peptides derived from neuropeptide FF
            precursors: the involvement of insulin-degrading enzyme
  JOURNAL   Int J Mol Sci 15 (9), 16787-16799 (2014)
   PUBMED   25247577
  REMARK    GeneRIF: Insulin-degrading enzyme is able to degrade specific amino
            acid sequences present in the neuropeptide pro-NPFFA (NPFF
            precursor), generating some cryptic peptides that are also observed
            after incubation with rat brain cortex homogenate.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 452)
  AUTHORS   Jaszberenyi,M., Bagosi,Z., Csabafi,K., Palotai,M. and Telegdy,G.
  TITLE     The actions of neuropeptide SF on the
            hypothalamic-pituitary-adrenal axis and behavior in rats
  JOURNAL   Regul Pept 188, 46-51 (2014)
   PUBMED   24316399
  REMARK    GeneRIF: NPSF may play a physiologic role in the regulation of such
            circadian functions as the activity of motor centers and the
            hypothalamic-pituitary-adrenal axis, through the release of
            corticotropin releasing hormone.
REFERENCE   5  (bases 1 to 452)
  AUTHORS   Mercado,F., Lopez,I., Ortega,A., Almanza,A., Soto,E. and Vega,R.
  TITLE     FMRFamide-related peptide expression in the vestibular-afferent
            neurons
  JOURNAL   Neurosci Lett 513 (1), 12-16 (2012)
   PUBMED   22342307
  REMARK    GeneRIF: the ASIC are tonically active in the vestibular-afferent
            neurons and modulated by RFamide-like peptides
REFERENCE   6  (bases 1 to 452)
  AUTHORS   Jhamandas,J.H. and Mactavish,D.
  TITLE     Central administration of neuropeptide FF (NPFF) causes increased
            neuronal activation and up-regulation of NPFF gene expression in
            the rat brainstem
  JOURNAL   J Comp Neurol 447 (3), 300-307 (2002)
   PUBMED   11984823
  REMARK    GeneRIF: NPFF is capable of up-regulating its own gene expression
            in the solitary nucleus.  NPFF may play a homeostatic role in
            response to specific cardiovascular challenges, such as
            hypotension.
REFERENCE   7  (bases 1 to 452)
  AUTHORS   Bonini,J.A., Jones,K.A., Adham,N., Forray,C., Artymyshyn,R.,
            Durkin,M.M., Smith,K.E., Tamm,J.A., Boteju,L.W., Lakhlani,P.P.,
            Raddatz,R., Yao,W.J., Ogozalek,K.L., Boyle,N., Kouranova,E.V.,
            Quan,Y., Vaysse,P.J., Wetzel,J.M., Branchek,T.A., Gerald,C. and
            Borowsky,B.
  TITLE     Identification and characterization of two G protein-coupled
            receptors for neuropeptide FF
  JOURNAL   J Biol Chem 275 (50), 39324-39331 (2000)
   PUBMED   11024015
REFERENCE   8  (bases 1 to 452)
  AUTHORS   Vilim,F.S., Aarnisalo,A.A., Nieminen,M.L., Lintunen,M.,
            Karlstedt,K., Kontinen,V.K., Kalso,E., States,B., Panula,P. and
            Ziff,E.
  TITLE     Gene for pain modulatory neuropeptide NPFF: induction in spinal
            cord by noxious stimuli
  JOURNAL   Mol Pharmacol 55 (5), 804-811 (1999)
   PUBMED   10220558
REFERENCE   9  (bases 1 to 452)
  AUTHORS   Devillers,J.P., Labrouche,S.A., Castes,E. and Simonnet,G.
  TITLE     Release of neuropeptide FF, an anti-opioid peptide, in rat spinal
            cord slices is voltage- and Ca(2+)-sensitive: possible involvement
            of P-type Ca2+ channels
  JOURNAL   J Neurochem 64 (4), 1567-1575 (1995)
   PUBMED   7891084
REFERENCE   10 (bases 1 to 452)
  AUTHORS   Kivipelto,L.
  TITLE     Ultrastructural localization of neuropeptide FF, a new neuropeptide
            in the brain and pituitary of rats
  JOURNAL   Regul Pept 34 (3), 211-224 (1991)
   PUBMED   1924889
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF148700.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF148700.1, BM385318.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132291, SAMD00132292
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..452
                     /gene="Npff"
                     /note="neuropeptide FF-amide peptide precursor"
                     /db_xref="GeneID:60337"
                     /db_xref="RGD:62033"
     exon            1..115
                     /gene="Npff"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..358
                     /gene="Npff"
                     /note="pain modulating peptide precursor;
                     FMRFamide-related peptides; pro-FMRFamide-related
                     neuropeptide FF"
                     /codon_start=1
                     /product="pro-FMRFamide-related neuropeptide FF
                     preproprotein"
                     /protein_id="NP_072108.1"
                     /db_xref="GeneID:60337"
                     /db_xref="RGD:62033"
                     /translation="MDSKWAAVLLLLLLLRNWGHAEEAGSWGEDQVFAEEDKGPHPSQ
                     YAHTPDRIQTPGSLMRVLLQAMERPRRNPAFLFQPQRFGRNAWGPWSKEQLSPQAREF
                     WSLAAPQRFGKK"
     exon            116..255
                     /gene="Npff"
                     /inference="alignment:Splign:2.1.0"
     exon            256..443
                     /gene="Npff"
                     /inference="alignment:Splign:2.1.0"
     regulatory      421..426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Npff"
     polyA_site      443
                     /gene="Npff"
ORIGIN      
        1 ggcagatggc agcatggatt ccaagtgggc tgctgtgctg ctgctactgc tgctgctgag
       61 gaactggggc cacgctgaag aggcagggag ctggggtgaa gaccaagtct ttgcagaaga
      121 agataaggga ccccacccat cacagtatgc ccacactcca gacaggatcc agactcctgg
      181 gtccctcatg cgtgttctgc tccaggccat ggagagacct agaaggaacc cagccttctt
      241 gtttcagccc cagaggtttg gcagaaatgc ttgggggccc tggagcaagg agcagctaag
      301 tccccaggct agagagttct ggagcctggc cgctcctcag cgctttggga agaagtaaca
      361 tcatctgctg tgatatgtct gcatgcaaaa tcacttcccc acattccatg tgtgccccca
      421 aataaagtgg tctggcttct gcaaaaaaaa aa
//
