LOCUS       NM_153624               5161 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus NPC intracellular cholesterol transporter 1
            (Npc1), mRNA.
ACCESSION   NM_153624 XM_002725312 XM_002728521
VERSION     NM_153624.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Guix,F.X., Capitan,A.M., Casadome-Perales,A., Palomares-Perez,I.,
            Lopez Del Castillo,I., Miguel,V., Goedeke,L., Martin,M.G.,
            Lamas,S., Peinado,H., Fernandez-Hernando,C. and Dotti,C.G.
  TITLE     Increased exosome secretion in neurons aging in vitro by
            NPC1-mediated endosomal cholesterol buildup
  JOURNAL   Life Sci Alliance 4 (8), e202101055 (2021)
   PUBMED   34183444
  REMARK    GeneRIF: Increased exosome secretion in neurons aging in vitro by
            NPC1-mediated endosomal cholesterol buildup.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Li,X., Lu,F., Trinh,M.N., Schmiege,P., Seemann,J., Wang,J. and
            Blobel,G.
  TITLE     3.3 A structure of Niemann-Pick C1 protein reveals insights into
            the function of the C-terminal luminal domain in cholesterol
            transport
  JOURNAL   Proc Natl Acad Sci U S A 114 (34), 9116-9121 (2017)
   PUBMED   28784760
REFERENCE   3  (bases 1 to 5161)
  AUTHORS   Demais,V., Barthelemy,A., Perraut,M., Ungerer,N., Keime,C.,
            Reibel,S. and Pfrieger,F.W.
  TITLE     Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons
            by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions
  JOURNAL   J Neurosci 36 (30), 8012-8025 (2016)
   PUBMED   27466344
  REMARK    GeneRIF: Here, we identify lamellar inclusions as the subcellular
            site of lipid accumulation in neurons, we uncover a vicious cycle
            of cholesterol synthesis and accretion, which may cause gradual
            neurodegeneration, and we reveal how beta-cyclodextrin, a potential
            therapeutic drug, reverts these changes. Our study provides new
            mechanistic insight in NPC disease and uncovers new targets for
            therapeutic approaches.
REFERENCE   4  (bases 1 to 5161)
  AUTHORS   Gong,X., Qian,H., Zhou,X., Wu,J., Wan,T., Cao,P., Huang,W.,
            Zhao,X., Wang,X., Wang,P., Shi,Y., Gao,G.F., Zhou,Q. and Yan,N.
  TITLE     Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated
            Cholesterol Transfer and Ebola Infection
  JOURNAL   Cell 165 (6), 1467-1478 (2016)
   PUBMED   27238017
REFERENCE   5  (bases 1 to 5161)
  AUTHORS   Domon,M.M., Besson,F., Tylki-Szymanska,A., Bandorowicz-Pikula,J.
            and Pikula,S.
  TITLE     Interaction of AnxA6 with isolated and artificial lipid
            microdomains; importance of lipid composition and calcium content
  JOURNAL   Mol Biosyst 9 (4), 668-676 (2013)
   PUBMED   23360953
REFERENCE   6  (bases 1 to 5161)
  AUTHORS   Blom,T.S., Linder,M.D., Snow,K., Pihko,H., Hess,M.W., Jokitalo,E.,
            Veckman,V., Syvanen,A.C. and Ikonen,E.
  TITLE     Defective endocytic trafficking of NPC1 and NPC2 underlying
            infantile Niemann-Pick type C disease
  JOURNAL   Hum Mol Genet 12 (3), 257-272 (2003)
   PUBMED   12554680
REFERENCE   7  (bases 1 to 5161)
  AUTHORS   Davies,J.P. and Ioannou,Y.A.
  TITLE     Topological analysis of Niemann-Pick C1 protein reveals that the
            membrane orientation of the putative sterol-sensing domain is
            identical to those of 3-hydroxy-3-methylglutaryl-CoA reductase and
            sterol regulatory element binding protein cleavage-activating
            protein
  JOURNAL   J Biol Chem 275 (32), 24367-24374 (2000)
   PUBMED   10821832
REFERENCE   8  (bases 1 to 5161)
  AUTHORS   Garver,W.S., Heidenreich,R.A., Erickson,R.P., Thomas,M.A. and
            Wilson,J.M.
  TITLE     Localization of the murine Niemann-Pick C1 protein to two distinct
            intracellular compartments
  JOURNAL   J Lipid Res 41 (5), 673-687 (2000)
   PUBMED   10787428
REFERENCE   9  (bases 1 to 5161)
  AUTHORS   Watari,H., Blanchette-Mackie,E.J., Dwyer,N.K., Glick,J.M.,
            Patel,S., Neufeld,E.B., Brady,R.O., Pentchev,P.G. and Strauss,J.F.
            3rd.
  TITLE     Niemann-Pick C1 protein: obligatory roles for N-terminal domains
            and lysosomal targeting in cholesterol mobilization
  JOURNAL   Proc Natl Acad Sci U S A 96 (3), 805-810 (1999)
   PUBMED   9927649
REFERENCE   10 (bases 1 to 5161)
  AUTHORS   Carstea,E.D., Morris,J.A., Coleman,K.G., Loftus,S.K., Zhang,D.,
            Cummings,C., Gu,J., Rosenfeld,M.A., Pavan,W.J., Krizman,D.B.,
            Nagle,J., Polymeropoulos,M.H., Sturley,S.L., Ioannou,Y.A.,
            Higgins,M.E., Comly,M., Cooney,A., Brown,A., Kaneski,C.R.,
            Blanchette-Mackie,E.J., Dwyer,N.K., Neufeld,E.B., Chang,T.Y.,
            Liscum,L., Strauss,J.F. 3rd, Ohno,K., Zeigler,M., Carmi,R.,
            Sokol,J., Markie,D., O'Neill,R.R., van Diggelen,O.P., Elleder,M.,
            Patterson,M.C., Brady,R.O., Vanier,M.T., Pentchev,P.G. and
            Tagle,D.A.
  TITLE     Niemann-Pick C1 disease gene: homology to mediators of cholesterol
            homeostasis
  JOURNAL   Science 277 (5323), 228-231 (1997)
   PUBMED   9211849
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On or before Nov 11, 2010 this sequence version replaced
            XM_002728521.1, XM_002725312.1, NM_153624.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360194.523720.1,
                                           SRR26643297.49392.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-155               JAXUCZ010000018.1  3699646-3699800     c
            156-278             JAXUCZ010000018.1  3684780-3684902     c
            279-385             JAXUCZ010000018.1  3683494-3683600     c
            386-561             JAXUCZ010000018.1  3681339-3681514     c
            562-729             JAXUCZ010000018.1  3678105-3678272     c
            730-979             JAXUCZ010000018.1  3676847-3677096     c
            980-1053            JAXUCZ010000018.1  3675783-3675856     c
            1054-1424           JAXUCZ010000018.1  3674999-3675369     c
            1425-1651           JAXUCZ010000018.1  3673964-3674190     c
            1652-1752           JAXUCZ010000018.1  3671582-3671682     c
            1753-1855           JAXUCZ010000018.1  3668568-3668670     c
            1856-2045           JAXUCZ010000018.1  3666001-3666190     c
            2046-2228           JAXUCZ010000018.1  3665474-3665656     c
            2229-2343           JAXUCZ010000018.1  3664746-3664860     c
            2344-2471           JAXUCZ010000018.1  3663486-3663613     c
            2472-2612           JAXUCZ010000018.1  3663262-3663402     c
            2613-2702           JAXUCZ010000018.1  3662480-3662569     c
            2703-2893           JAXUCZ010000018.1  3661932-3662122     c
            2894-3009           JAXUCZ010000018.1  3661371-3661486     c
            3010-3139           JAXUCZ010000018.1  3660737-3660866     c
            3140-3340           JAXUCZ010000018.1  3659278-3659478     c
            3341-3572           JAXUCZ010000018.1  3658311-3658542     c
            3573-3686           JAXUCZ010000018.1  3657486-3657599     c
            3687-3849           JAXUCZ010000018.1  3656250-3656412     c
            3850-5161           JAXUCZ010000018.1  3654237-3655548     c
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p13"
     gene            1..5161
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /note="NPC intracellular cholesterol transporter 1"
                     /db_xref="GeneID:266732"
                     /db_xref="RGD:628693"
     exon            1..155
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..3932
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /note="Niemann-Pick disease, type C1; solute carrier
                     family 66 member 1"
                     /codon_start=1
                     /product="NPC intracellular cholesterol transporter 1
                     precursor"
                     /protein_id="NP_705888.2"
                     /db_xref="GeneID:266732"
                     /db_xref="RGD:628693"
                     /translation="MGAHHPALGLLLLLLLCPAQVFSQSCVWYGECGVAFGDKRYNCE
                     YSGPPKPLPKDGYDLVQELCPGLFFGNVSLCCDVQQLQTLKSNLQLPLQFLSRCPSCF
                     YNLMTLFCELTCSPHQSQFLNVTATEDYFDPETRENKTNVKELEYYVGRSFANAMYNA
                     CRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVFSDLSVLGM
                     EPMRNATKGCNESVDEVTGPCSCQDCSAVCGPKPQPPPPPVPWRILGLDAMYVIMWVA
                     YMAFLVLFFGGLLAVWCHRRRYFVSEYTPIDSNIAFSSNSSDKGEASCCDPLGVAFDD
                     CLRRMFTKWGAFCVRNPTCIIFFSLVFIAACSSGLVFVQVTTNPVELWSAPHSQARLE
                     KEYFDKHFGPFFRTEQLIIQAPNSSVHIYEPYPSGADVPFGPPLNKEILHQVLDLQIA
                     IESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQNSHAVLDNQVGDDFFVYA
                     DYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITF
                     PVNNYYNDTEKLQRAWAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESNSDVFT
                     VLISYAVMFLYISLALGHIQSWNRVLVDSKISLGIAGILIVLSSVTCSLGIFSYIGMP
                     LTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSS
                     FSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSFLGLDIKRQEKNRLDI
                     LCCVRGPDDGQESQASESYLFRFFKNAFAPFLLTDWLRPVVMAVFVGILSFSIAVVNK
                     VEIGLDQSLSMPNDSYVIDYFKSLGQYLHSGPPVYFVLEEGYNYSSRRGQNMVCGGMG
                     CDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASV
                     IDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYSSAVNIMGDD
                     TYIGATYFMTYHTILKTSADYIDALKKARLIASNITETMRSKGSDYRVFPYSVFYVFY
                     EQYLTIIDDAIFNLSVSLGSIFLVTLVALGCELWSAVIMCLTIAMILVNMFGVMWLWG
                     ISLNAVSLVNLVMTCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITL
                     TKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKRHT
                     TQERYKGTERERLLNF"
     exon            156..278
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            279..385
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            386..561
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            562..729
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            730..979
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1053
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1424
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1651
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1752
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1855
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..2045
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2228
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2343
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2471
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2472..2612
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2613..2702
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2893
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..3009
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3010..3139
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3340
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3572
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3573..3686
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3687..3849
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
     exon            3850..5161
                     /gene="Npc1"
                     /gene_synonym="Cdig2; SLC66A1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgccggtcc cgaaaccact cggccagtgc tgtggcgctc gggtcgcggt gcactgcgag
       61 ccgtaccgag agctgtagcc ccgcgcggcg acagcatggg cgcgcaccac ccggccctcg
      121 gcctcctgct gctgctgctg ctgtgccctg cgcaggtgtt ctcacagtcc tgtgtctggt
      181 acggagagtg tggagtcgcc tttggagata agaggtacaa ctgtgaatat tcgggcccac
      241 cgaaacccct cccaaaggac ggctatgact tagtgcagga actctgtcca ggactcttct
      301 ttggcaatgt cagtctttgc tgtgacgttc agcagcttca gacactgaag agcaacctgc
      361 agctgcccct gcagtttctg tccaggtgtc cgtcatgttt ttataacctg atgaccctgt
      421 tttgtgagct aacatgtagc ccgcaccaga gtcagtttct gaatgtgaca gcaactgaag
      481 attatttcga ccccgagact cgggaaaata aaacaaatgt aaaggaatta gagtactacg
      541 tcggacggag ctttgcgaat gcaatgtaca atgcctgccg agatgtggag gcgccttcca
      601 gtaatgagaa ggccttagga ctcctgtgcg gaagggatgc ccgtgcctgc aatgccacca
      661 actggattga gtacatgttc aacaaagaca acgggcaagc accgtttacc atcatccccg
      721 tattttcaga tctttcggtt cttggaatgg aacccatgag gaatgccacc aaaggctgca
      781 acgagtctgt agatgaggtc acagggccgt gtagctgcca ggactgctct gccgtctgcg
      841 gccccaaacc ccagccccca ccgccccctg tgccctggag gatcttaggt ttggacgcca
      901 tgtatgtcat catgtgggtc gcctacatgg cgttcctggt cctgttcttt ggaggactgc
      961 tggcagtgtg gtgccacaga aggcggtact ttgtgtccga gtacaccccc attgacagca
     1021 acattgcctt ttcctcgaat agcagtgaca aaggggaagc ctcgtgctgc gacccacttg
     1081 gtgtagcgtt tgatgactgt ctgaggcgca tgttcacaaa gtggggggct ttctgtgtcc
     1141 ggaatcccac ctgcatcatt ttcttctcgt tggtcttcat tgctgcgtgc tcttctggcc
     1201 tggtgtttgt ccaggtcacc accaatcccg tggagctctg gtcagcccct cacagtcagg
     1261 cccgcttgga aaaggagtac tttgacaagc actttgggcc tttctttcgt acggagcagc
     1321 ttattatcca ggcccccaac tccagcgtcc atatctacga gccgtaccct tccggagccg
     1381 atgtgccctt cgggcctcca ttgaacaaag agattctgca ccaggttctg gacttacaga
     1441 tcgccattga aagtatcact gcgtcctata acaatgaaac cgtgacactt caggacatct
     1501 gtgtggcccc cctctctcca tacaacaaga actgcaccat tatgagtgtg ttaaattact
     1561 tccagaacag ccatgcggtg ctggacaacc aagtaggcga tgacttcttt gtctatgccg
     1621 attaccacac gcactttctg tactgtgtga gggctcccgc ctccttgaat gatacgagtt
     1681 tgctccacgg cccttgcctg ggcacatttg gaggacctgt gttcccgtgg cttgtgttgg
     1741 gcggctatga tgatcagaac tacaataatg ccaccgcgct tgtgattacc ttccctgtca
     1801 ataattacta taatgacaca gagaagctcc agagggcctg ggcctgggag aaagaattta
     1861 ttaactttgt gaagaactac aagaatccaa atctgaccat ttctttcact gctgagcgaa
     1921 gcattgaaga tgaactcaat cgggaaagca acagtgacgt attcaccgtc ctcatcagct
     1981 atgccgtgat gtttctgtac atttccctcg ccctggggca catccagagc tggaacaggg
     2041 tcctggtgga ttctaaaatc tcgctgggca ttgcggggat cctgatagtg ctgagctcgg
     2101 tgacctgctc cctgggcatc ttcagctaca tcggaatgcc actgaccctc atcgtcattg
     2161 aggtcatccc gttcctggtg ctggctgttg gggtggacaa catcttcatt ctagtgcaga
     2221 cctaccagag agatgagcgt cttcaggagg agacgttgga tcagcagctg ggcaggatcc
     2281 tgggggaagt ggccccaacc atgttccttt cgtccttctc tgagacctcg gcatttttct
     2341 ttggggcgct gtcctcgatg ccagctgtcc acaccttctc tctgtttgcg gggatggccg
     2401 tcctcattga cttcctcctt cagattacct gctttgtgag cttcttgggg ttagatatta
     2461 agagacaaga gaaaaaccgt ctggacatcc tgtgctgtgt cagaggccct gacgacggac
     2521 aagaatccca agcctccgaa agctacctgt tccgcttctt caaaaacgcc tttgccccat
     2581 ttctgctgac ggactggctg cggccagttg tgatggccgt gtttgtgggc attctgtcat
     2641 tcagtattgc agtagtgaac aaagtagaaa tcggattgga tcagtctctc tcgatgccaa
     2701 atgattcgta tgtgattgac tatttcaagt cactcggtca gtacctgcac tcgggcccac
     2761 ccgtgtactt cgtcctggaa gagggctaca actacagttc ccgcagaggg cagaacatgg
     2821 tgtgcggtgg catgggctgt gacaatgact ccctggtgca gcagatattt aacgcagcag
     2881 agctggacac ctacacccgc gtaggcttcg ctccctcatc ctggattgat gattactttg
     2941 actgggtctc accgcaatcc tcctgctgta gactctacaa tgtcactcac cagttctgca
     3001 atgcttctgt gattgaccca acctgtgtcc gctgcagacc tctgactcca gagggcaaac
     3061 agaggcctca ggggaaagaa ttcatgaaat tcctgcccat gttcctgtct gataacccca
     3121 accccaagtg tggcaaaggg ggacatgctg cttacagttc tgctgttaac atcatgggag
     3181 atgacactta cattggggcc acttacttca tgacctacca caccattctt aagacctctg
     3241 ctgattatat cgatgccctg aagaaagctc ggcttatagc cagtaacatc acggaaacca
     3301 tgcgttctaa ggggagtgat tatcgtgtgt ttccttacag tgtgttctat gtcttctatg
     3361 aacaatacct gaccattatt gatgacgcca tctttaacct aagtgtgtct ctgggctcca
     3421 tatttctcgt gaccttggtg gctctgggct gtgaactgtg gtctgcggtc atcatgtgtc
     3481 tcaccatagc tatgatcctg gtcaacatgt ttggtgttat gtggctgtgg ggcatcagtc
     3541 tgaacgctgt ttccttggtc aacttggtga tgacctgtgg catttctgtg gagttctgca
     3601 gccatataac aagagctttc accatgagca ccaaaggaag cagagtgagc agggcggaag
     3661 aggcactggc ccacatgggt agttctgtat tcagtggaat cacacttaca aaatttggag
     3721 ggatcgtggt gttggccttt gccaagtctc aaatttttga gatcttttac ttcaggatgt
     3781 acttagccat ggtcttactt ggagctactc acggactaat atttcttccc gtcttactca
     3841 gttacatagg gccatcagtg aataaagcta aaagacacac cactcaagag cggtacaaag
     3901 ggacagagag agaacggctc ctcaattttt agccatgtag cagactttgg tgactttgcg
     3961 tctatggata ggtcaagttt actgcgggac agctgcactg tcaaggctga gctggctgct
     4021 ggttgttccc aggagttagg ctgtgtcaca gctgtggcaa atgcaactcc actcactctt
     4081 ttagactcag gaatgcacac taacttgtga agcagtatta tggatctgaa ggcaacagtc
     4141 accagcccct acaggctaag aaacctcatc ttgggcacaa ggaagggcag ttctacaact
     4201 ttcattcttt caatgcctac ttttaatgat ggctatcaaa ccatcattaa aactttaacc
     4261 ccttctagtc caacacccac cagagagtgg gaagtggatg tgttaagaaa ggttctttgc
     4321 agcatatcct atctgtgttg tcaggaaatc agcagttcag ttcataggtc agcaactcga
     4381 tcactcaaac acctcacgga tacatcagct gtccagttca atagtatcgg gatggcagca
     4441 gcagtggcca ggcctcggct gaatggccat cagcaggagg ggcggggcca acaggaactc
     4501 tcgatgctct ctgctgagcc tctcacagcg aagactcaag acccaacaaa tgttccacag
     4561 cttagctctc tatttccttc tctgtggagt cccaacaaac agagctcagc tacgcaatgt
     4621 aaggcagacc aagcaataca tgccagtcat tcatgtcctt tcacgtgctt gctttaggga
     4681 aacagtcctt cacaagtctg ccttagcctt tcacctgtgt ctgccccagc aaaacaccaa
     4741 cacaactgac tgtccacttc acaggtggtg gctacaactg tgacctgatc cccaattctg
     4801 aaaagccagc ccacatgctc tctcctgttt tgggagtcag caacatttga ggatgctgta
     4861 ggtactttat tacaacactt ttgtagttta aagagcttta ttaatgcaat aaattaactt
     4921 tgtacatttt tatattaaaa ccgttgaggg acttcagaat gttgtaggcc tcattagagc
     4981 ttgttctcca aaagcctgct tgaaaaaggc tacgtgttcc tcacagtgtt ctcctgcagg
     5041 aaaaatgtag cttaggtgcc agctggttgt ggcacaggaa gacggagtgc agacaatgct
     5101 catgggttgt atgctgggtt ttaacttatt tttctttaat aaaatccctc ctacattttg
     5161 a
//
