LOCUS       NM_153293                514 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus neuropeptide B (Npb), mRNA.
ACCESSION   NM_153293
VERSION     NM_153293.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Pandey,S., Tuma,Z., Peroni,E., Monasson,O., Papini,A.M. and
            Chottova Dvorakova,M.
  TITLE     Identification of NPB, NPW and Their Receptor in the Rat Heart
  JOURNAL   Int J Mol Sci 21 (21), 7827 (2020)
   PUBMED   33105700
  REMARK    GeneRIF: Identification of NPB, NPW and Their Receptor in the Rat
            Heart.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  AUTHORS   Skrzypski,M., Pruszynska-Oszmalek,E., Rucinski,M.,
            Szczepankiewicz,D., Sassek,M., Wojciechowicz,T., Kaczmarek,P.,
            Kolodziejski,P.A., Strowski,M.Z., Malendowicz,L.K. and Nowak,K.W.
  TITLE     Neuropeptide B and W regulate leptin and resistin secretion, and
            stimulate lipolysis in isolated rat adipocytes
  JOURNAL   Regul Pept 176 (1-3), 51-56 (2012)
   PUBMED   22484289
  REMARK    GeneRIF: The Neuropeptide B can affect glucose and lipid
            homeostasis, and food intake in rats.
REFERENCE   3  (bases 1 to 514)
  AUTHORS   Ziolkowska,A., Rucinski,M., Tyczewska,M. and Malendowicz,L.K.
  TITLE     Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured
            rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit
            proliferative activity of ROB cells
  JOURNAL   Int J Mol Med 24 (6), 781-787 (2009)
   PUBMED   19885618
  REMARK    GeneRIF: RT-PCR technique revealed the presence of NPB mRNA, NPW
            mRNA, and NPBWR1 mRNA, but not similar to NPBWR2 mRNA in ROB cells.
REFERENCE   4  (bases 1 to 514)
  AUTHORS   Schulz,S., Stumm,R. and Hollt,V.
  TITLE     Immunofluorescent identification of neuropeptide B-containing nerve
            fibers and terminals in the rat hypothalamus
  JOURNAL   Neurosci Lett 411 (1), 67-71 (2007)
   PUBMED   17067739
  REMARK    GeneRIF: NPB-immunoreactivity was enriched in many regions within
            the hypothalamus which also contained high levels of GPR7 mRNA.
REFERENCE   5  (bases 1 to 514)
  AUTHORS   Jackson,V.R., Lin,S.H., Wang,Z., Nothacker,H.P. and Civelli,O.
  TITLE     A study of the rat neuropeptide B/neuropeptide W system using in
            situ techniques
  JOURNAL   J Comp Neurol 497 (3), 367-383 (2006)
   PUBMED   16736466
  REMARK    GeneRIF: demonstrates the expression patterns of GPR7, NPB, and NPW
            precursor messenger ribonucleic acid (mRNA) in the rat brain by
            using in situ hybridization and in situ binding experiments
REFERENCE   6  (bases 1 to 514)
  AUTHORS   Fujii,R., Yoshida,H., Fukusumi,S., Habata,Y., Hosoya,M.,
            Kawamata,Y., Yano,T., Hinuma,S., Kitada,C., Asami,T., Mori,M.,
            Fujisawa,Y. and Fujino,M.
  TITLE     Identification of a neuropeptide modified with bromine as an
            endogenous ligand for GPR7
  JOURNAL   J Biol Chem 277 (37), 34010-34016 (2002)
   PUBMED   12118011
  REMARK    GeneRIF: neuropeptide B, a neuropeptide modified with bromine is an
            endogenous ligand for GPR7.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Oct 27, 2022 this sequence version replaced NM_153293.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BG376816.1, AB085944.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-231               JAXUCZ010000010.1  106384978-106385208
            232-514             JAXUCZ010000010.1  106385284-106385566
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q32.3"
     gene            1..514
                     /gene="Npb"
                     /note="neuropeptide B"
                     /db_xref="GeneID:259222"
                     /db_xref="RGD:708513"
     CDS             1..360
                     /gene="Npb"
                     /note="rPPL7; preproprotein L7; preproneuropeptide B"
                     /codon_start=1
                     /product="neuropeptide B precursor"
                     /protein_id="NP_695205.1"
                     /db_xref="GeneID:259222"
                     /db_xref="RGD:708513"
                     /translation="MVRCRTLVAAALALLLTPALAWYKPAAGSHHYSVGRAAGLLSSF
                     HRFPSTRRSESPALRVGTVPLRNLEMRPSVRSLALCVKDVTPNLQSCQRQLNSRGTFQ
                     CKADVFLSLHKAECQSA"
     exon            1..231
                     /gene="Npb"
                     /inference="alignment:Splign:2.1.0"
     exon            232..514
                     /gene="Npb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggtccggt gtaggacgct ggtggccgcc gccctggcgc tgctcctgac gccagccctc
       61 gcgtggtaca agcccgcggc gggatcccac cactactcgg tgggccgcgc tgcggggcta
      121 ctgtcgagtt tccacaggtt cccatccacg cgacgttccg agtctccagc actccgggtg
      181 ggaaccgtac ctctgcgcaa cttggagatg cgcccaagcg taagaagcct tgccctgtgt
      241 gtcaaagatg tgaccccgaa cctgcagagc tgccagcggc aactcaacag ccgagggact
      301 ttccagtgta aggcggacgt cttcttgtcg ctgcacaagg ctgaatgcca aagcgcctga
      361 accccaaccc aaacctcgac gctgcggcca ctgactcccc ctcccagcca cccccttact
      421 cccagtcttg ctagcacagg ccctgtgtga tttggctcct gtggccccta actcccaccg
      481 agctccaaaa tgaataaaga agcaaaaccg tctc
//
