LOCUS       NM_021838               3953 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Rattus norvegicus nitric oxide synthase 3 (Nos3), mRNA.
ACCESSION   NM_021838 XM_342615
VERSION     NM_021838.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Babiker,F. and Al-Kouh,A.
  TITLE     Immunoglobulin-Mediated Cardiac Protection From
            Ischemia/Reperfusion Injury in Diabetic Rats Is Associated With
            Endothelial Nitric Oxide Synthase/Glucose Transporter-4 Signaling
            Pathway
  JOURNAL   J Cardiovasc Pharmacol 84 (3), 319-330 (2024)
   PUBMED   39240727
  REMARK    GeneRIF: Immunoglobulin-Mediated Cardiac Protection From
            Ischemia/Reperfusion Injury in Diabetic Rats Is Associated With
            Endothelial Nitric Oxide Synthase/Glucose Transporter-4 Signaling
            Pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Sakaguchi,R., Takahashi,N., Yoshida,T., Ogawa,N., Ueda,Y.,
            Hamano,S., Yamaguchi,K., Sawamura,S., Yamamoto,S., Hara,Y.,
            Kawamoto,T., Suzuki,R., Nakao,A., Mori,M.X., Furukawa,T.,
            Shimizu,S., Inoue,R. and Mori,Y.
  TITLE     Dynamic remodeling of TRPC5 channel-caveolin-1-eNOS protein
            assembly potentiates the positive feedback interaction between Ca2+
            and NO signals
  JOURNAL   J Biol Chem 300 (9), 107705 (2024)
   PUBMED   39178948
  REMARK    GeneRIF: Dynamic remodeling of TRPC5 channel-caveolin-1-eNOS
            protein assembly potentiates the positive feedback interaction
            between Ca[2+] and NO signals.
REFERENCE   3  (bases 1 to 3953)
  AUTHORS   Wang,N., Jiang,Q., Xie,L., Cheng,B., Liu,Q.W. and Jiang,R.
  TITLE     Methylation of eNOS in the rat penile corpus cavernosum under
            different pathological states and its relationship with erectile
            function
  JOURNAL   Andrology 12 (1), 222-230 (2024)
   PUBMED   37222247
  REMARK    GeneRIF: Methylation of eNOS in the rat penile corpus cavernosum
            under different pathological states and its relationship with
            erectile function.
REFERENCE   4  (bases 1 to 3953)
  AUTHORS   Wang,J., Zhan,H., Wang,M., Song,H., Sun,J. and Zhao,G.
  TITLE     Sonic hedgehog signaling promotes angiogenesis of endothelial
            progenitor cells to improve pressure ulcers healing by
            PI3K/AKT/eNOS signaling
  JOURNAL   Aging (Albany NY) 15 (19), 10540-10548 (2023)
   PUBMED   37815888
  REMARK    GeneRIF: Sonic hedgehog signaling promotes angiogenesis of
            endothelial progenitor cells to improve pressure ulcers healing by
            PI3K/AKT/eNOS signaling.
            Erratum:[Aging (Albany NY). 2023 Dec 29;15(24):15703-15704. doi:
            10.18632/aging.205458.. PMID: 38157439]
REFERENCE   5  (bases 1 to 3953)
  AUTHORS   Refaie,M.M.M., El-Hussieny,M., Shehata,S., Welson,N.N. and
            Abdelzaher,W.Y.
  TITLE     Exploring the role of ATP-sensitive potassium channel, eNOS, and
            P-glycoprotein in mediating the hepatoprotective activity of
            nicorandil in methotrexate-induced liver injury in rats
  JOURNAL   Immunopharmacol Immunotoxicol 45 (5), 607-615 (2023)
   PUBMED   37078892
  REMARK    GeneRIF: Exploring the role of ATP-sensitive potassium channel,
            eNOS, and P-glycoprotein in mediating the hepatoprotective activity
            of nicorandil in methotrexate-induced liver injury in rats.
REFERENCE   6  (bases 1 to 3953)
  AUTHORS   Chen,P.F., Tsai,A.L. and Wu,K.K.
  TITLE     Cysteine 99 of endothelial nitric oxide synthase (NOS-III) is
            critical for tetrahydrobiopterin-dependent NOS-III stability and
            activity
  JOURNAL   Biochem Biophys Res Commun 215 (3), 1119-1129 (1995)
   PUBMED   7488039
REFERENCE   7  (bases 1 to 3953)
  AUTHORS   Deng,A.Y. and Rapp,J.P.
  TITLE     Locus for the inducible, but not a constitutive, nitric oxide
            synthase cosegregates with blood pressure in the Dahl
            salt-sensitive rat
  JOURNAL   J Clin Invest 95 (5), 2170-2177 (1995)
   PUBMED   7537756
REFERENCE   8  (bases 1 to 3953)
  AUTHORS   Kawai,N., Bloch,D.B., Filippov,G., Rabkina,D., Suen,H.C.,
            Losty,P.D., Janssens,S.P., Zapol,W.M., de la Monte,S. and
            Bloch,K.D.
  TITLE     Constitutive endothelial nitric oxide synthase gene expression is
            regulated during lung development
  JOURNAL   Am J Physiol 268 (4 Pt 1), L589-L595 (1995)
   PUBMED   7537461
REFERENCE   9  (bases 1 to 3953)
  AUTHORS   Mohaupt,M.G., Elzie,J.L., Ahn,K.Y., Clapp,W.L., Wilcox,C.S. and
            Kone,B.C.
  TITLE     Differential expression and induction of mRNAs encoding two
            inducible nitric oxide synthases in rat kidney
  JOURNAL   Kidney Int 46 (3), 653-665 (1994)
   PUBMED   7527874
REFERENCE   10 (bases 1 to 3953)
  AUTHORS   Janssens,S.P., Shimouchi,A., Quertermous,T., Bloch,D.B. and
            Bloch,K.D.
  TITLE     Cloning and expression of a cDNA encoding human endothelium-derived
            relaxing factor/nitric oxide synthase
  JOURNAL   J Biol Chem 267 (21), 14519-14522 (1992)
   PUBMED   1378832
  REMARK    Erratum:[J Biol Chem. 1992 Nov 5;267(31):22694.. PMID: 1385404]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB176831.1.
            
            On or before Aug 30, 2004 this sequence version replaced
            XM_342615.1, NM_021838.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB176831.1, AY695391.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q11"
     gene            1..3953
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /note="nitric oxide synthase 3"
                     /db_xref="GeneID:24600"
                     /db_xref="RGD:3186"
     exon            1..170
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     CDS             7..3615
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /EC_number="1.14.13.39"
                     /note="nitric oxide synthase, endothelial; cNOS; EC-NOS;
                     NOSIII; NOS type III; endothelial NOS; constitutive NOS;
                     endothelial nitric oxide synthase 3; nitric oxide synthase
                     3, endothelial cell"
                     /codon_start=1
                     /product="nitric oxide synthase 3"
                     /protein_id="NP_068610.1"
                     /db_xref="GeneID:24600"
                     /db_xref="RGD:3186"
                     /translation="MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPVP
                     PSPTRPAPDHSPPLTRPPDGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSL
                     VFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRSGSQAHEQRLQEVEAEVVATG
                     TYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYICNHIKYAT
                     NRGNLRSAITVFPQRYAGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQH
                     GWTPGNGRFDVLPLLLQAPDEPPELFTLPPELVLEVPLEHPTLEWFAALGLRWYALPA
                     VSNMLLEIGGLEFPAAPFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSL
                     WKDKAAVEINVAVLYSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPP
                     ISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASL
                     MGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALV
                     LVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSS
                     WRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLG
                     QGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQA
                     ESLQLLPGLTHVHRRKMFQATILSVENLQSSKSTRATILVRLDTGSQEGLQYQPGDHI
                     GVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQ
                     ALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFRCPTLLEVL
                     EQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTVAVLAYRTQDVLGPLHY
                     GVCSTWMSQLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHD
                     IEIKGLQPAPMTLVFGCRCSQLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQ
                     DLLRTELAAEVHRVLCLEQGHMFVCGDVTMATSVLQTVQRILATEGSMELDEAGDVIG
                     VLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWSFDPPTQETPGS"
     exon            171..273
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            274..422
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            423..585
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            586..677
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            678..819
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            820..959
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1134
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1236
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1431
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1505
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1650
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1755
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1823
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1940
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2115
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2248
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2327
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2328..2515
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2688
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2899
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2900..2987
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            2988..3109
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            3110..3258
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3453
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3953
                     /gene="Nos3"
                     /gene_synonym="eNos"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctggcatgg gcaacttgaa gagtgtgggc caggagcctg ggccaccctg tggcctaggg
       61 ctcgggctgg gcctagggct atgcggcaag cagggcccag cctcaccggc accagagcct
      121 agccaggcac cagtaccccc gtccccaacc cgaccagcac cagaccacag ccccccgtta
      181 acccggcccc cagacggacc caagtttcct cgagtaaaga actgggaagt gggcagcatc
      241 acctacgata ccctcagtgc acaggctcag caggatgggc cctgtacccc aagacgctgc
      301 ttgggatccc tggtatttcc aaggaagtta cagagccggc ccacccaggg cccttcaccc
      361 actgagcagc tattgggtca agcccgggac ttcatcaatc agtactataa ctcgatcaaa
      421 aggagtggtt cccaggctca tgagcagcgg cttcaggaag tggaagctga ggtggtggcc
      481 acgggcacct accagctccg ggagagtgag ctggtgtttg gggccaaaca ggcctggcgc
      541 aacgctcccc gctgtgtggg gcggatccag tgggggaaac tgcaggtatt tgatgctcgg
      601 gactgcagga cagcacagga aatgttcacc tacatctgta accacattaa gtatgcaaca
      661 aaccgaggca atcttcgttc agccatcacg gtgttccccc agcgctacgc tggccgggga
      721 gacttccgga tctggaacag ccagctggtg cgctacgcgg gctataggca gcaggacggc
      781 tctgtgcgag gggaccctgc caacgtggag atcactgagc tctgtattca acatggctgg
      841 accccaggaa atggccgctt tgatgtgctg cccctgctac tccaggctcc cgatgagccc
      901 ccagaactct tcactctgcc cccagagctg gtcctcgagg tgcctctgga gcaccccacg
      961 ctagagtggt ttgctgccct tggcctgcgc tggtatgccc tcccagctgt gtccaatatg
     1021 ctgctagaaa tcgggggcct ggagtttccg gctgcccctt tcagcggctg gtacatgagt
     1081 tcagagattg gcatgaggga cctgtgtgac cctcaccgat acaacatact tgaggatgtg
     1141 gctgtctgca tggatctaga cacccggaca acctcatcac tgtggaaaga caaggcagca
     1201 gtggaaatta acgtggctgt gctgtacagt taccagctgg ccaaagtgac cattgtggac
     1261 caccatgccg ccacagcctc cttcatgaag cacttggaaa atgagcagaa ggccagaggg
     1321 ggctgccccg ctgactgggc ctggatcgtg ccccccatct caggcagcct cacccctgtc
     1381 ttccatcaag agatggtcaa ctatttcctg tcccctgcct tccgctacca gcctgacccc
     1441 tggaaaggaa gtgcagcaaa aggcacaggc atcaccagga agaagacttt taaggaagta
     1501 gccaatgcag tgaagatctc tgcctcactc atgggcacgg tgatggcgaa gcgtgtgaag
     1561 gcgactatcc tgtatggctc tgagactggc cgcgcccaga gctacgcaca gcagctgggg
     1621 aggctctttc ggaaggcgtt tgacccccgg gtcctgtgca tggatgaata cgatgtggta
     1681 tccctagagc atgaggcctt ggtattggtg gtgaccagca catttggcaa tggggatccc
     1741 ccggagaatg gagagagctt tgcagcagcg ctgatggaaa tgtcgggccc ctacaacagc
     1801 tcccctcggc ctgagcagca caagagttac aaaatccgat tcaacagtgt ctcctgctcg
     1861 gacccactgg tatcctcttg gcggcgcaaa aggaaggaat ccagtaacac agacagtgca
     1921 ggggccctgg gcaccctcag gttctgtgtg tttgggctgg gctccagagc atacccgcac
     1981 ttctgtgcct ttgctcgagc ggtggacaca aggctggagg agctgggcgg ggagcgactg
     2041 ttgcagctgg gccagggtga tgagctctgc ggccaggagg aggctttccg aggctgggcc
     2101 caggcagcct tccaggctgc ctgtgaaact ttctgtgtgg gagaagatgc caaggctgct
     2161 gcccgagata tcttcagtcc caagcgcagc tggaaacgcc agaggtaccg gctgagtacc
     2221 caagctgaga gcctgcaatt actgccaggg ctgactcacg tgcacagacg gaagatgttc
     2281 caggctacaa ttctttctgt ggagaaccta cagagcagca aatccacccg agccacaatc
     2341 ctggtgcgtc tggacactgg aagccaggag ggactgcagt accagccagg ggaccacata
     2401 ggtgtgtgcc cacccaaccg gcctggccta gtggaggcgc tgctgagccg agtggaggac
     2461 cctccgccat ccacagagcc tgtggccgtg gaacaactgg aaaaaggcag ccctggtggc
     2521 cctccccccg gctgggtacg ggacccccgg cttcccccat gtacgctgcg ccaggctctc
     2581 acttacttcc tggacatcac ttccccgccc agccctcgcc ttcttcgact gctgagcacc
     2641 ctggcagagg agtccagcga acagcaggag ctagaggctc ttagccagga cccccggcgc
     2701 tacgaagaat ggaagtggtt ccgctgcccc acactgctag aggtgctgga acaatttcca
     2761 tccgtggcac tgcctgcccc gctgatcctc acccagctgc ccctgctcca gccccggtac
     2821 tactctgtca gctcagcacc cagcgcccac ccaggagaga tccacctcac tgtagctgtg
     2881 ctggcataca gaacccagga tgtgctgggc cctctgcact atggggtctg ttccacatgg
     2941 atgagccaac tcaaggcagg agacccggtg ccctgcttca tcaggggggc tccctccttc
     3001 cggctgccac ctgatcctaa cttgccttgc atcctggtgg gcccagggac tggtattgca
     3061 cccttccggg gattctggca agaccgatta cacgacattg agatcaaagg actgcagcct
     3121 gcccccatga ctttggtgtt tggctgccga tgctcccaac tggaccatct ctaccgggac
     3181 gaggtactgg acgcccagca gcgtggagtg tttggacaag tcctcaccgc cttttccagg
     3241 gatcctggca gccctaagac ctatgtgcaa gacctcctga ggacagagct ggccgcggag
     3301 gttcaccgcg tgctgtgcct cgagcaagga cacatgtttg tctgcggtga tgtcactatg
     3361 gcaaccagcg tcctgcaaac cgtgcagcga attctggcaa cagagggcag catggagctg
     3421 gatgaagccg gtgacgtcat cggcgtgctg cgggatcagc aacgctacca cgaggacatt
     3481 ttcggactca cattgcgcac ccaggaggtg acgagccgca tccgcaccca gagtttttct
     3541 ttgcaggagc gacagctgag gggtgcagtg ccctggtcct ttgacccgcc tacccaagaa
     3601 acacctggtt cctgacaatc ttcttcccct ccagttcccg gagagaggga ctgcgtcgct
     3661 tcattaggtt gaccaaggca aaccaccctc tctggaatgc ccacagcatc agttgcgagg
     3721 ctgccgattt aagaactctc ttctcggggc tggggattta gctcagtggt aaagcgcttg
     3781 cctaggaagc gcaaggccct gggttcggtc cccagctccg gaaaaaaaaa aaaactctct
     3841 tctctctcca gtttaactag actgggaggg agtcagccta aatcctgaag gttcctccgg
     3901 ctgtggtatt aggacatcct gctgccctct tcgtatgctt tgatggactg tag
//
