LOCUS       NM_001013157            4149 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus nicotinamide nucleotide transhydrogenase (Nnt),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001013157 XM_227084
VERSION     NM_001013157.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Picklo MJ and Thyfault JP.
  TITLE     Vitamin E and vitamin C do not reduce insulin sensitivity but
            inhibit mitochondrial protein expression in exercising obese rats
  JOURNAL   Appl Physiol Nutr Metab 40 (4), 343-352 (2015)
   PUBMED   25761734
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Lopert P and Patel M.
  TITLE     Nicotinamide nucleotide transhydrogenase (Nnt) links the substrate
            requirement in brain mitochondria for hydrogen peroxide removal to
            the thioredoxin/peroxiredoxin (Trx/Prx) system
  JOURNAL   J Biol Chem 289 (22), 15611-15620 (2014)
   PUBMED   24722990
  REMARK    GeneRIF: Nnt is the critical link between the metabolic and H2O2
            antioxidant function in brain mitochondria and is a potential
            therapeutic target to improve the redox balance in conditions of
            oxidative stress associated with neurodegenerative diseases.
REFERENCE   3  (bases 1 to 4149)
  AUTHORS   Meimaridou E, Kowalczyk J, Guasti L, Hughes CR, Wagner F, Frommolt
            P, Nurnberg P, Mann NP, Banerjee R, Saka HN, Chapple JP, King PJ,
            Clark AJ and Metherell LA.
  TITLE     Mutations in NNT encoding nicotinamide nucleotide transhydrogenase
            cause familial glucocorticoid deficiency
  JOURNAL   Nat Genet 44 (7), 740-742 (2012)
   PUBMED   22634753
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4149)
  AUTHORS   Yin F, Sancheti H and Cadenas E.
  TITLE     Silencing of nicotinamide nucleotide transhydrogenase impairs
            cellular redox homeostasis and energy metabolism in PC12 cells
  JOURNAL   Biochim Biophys Acta 1817 (3), 401-409 (2012)
   PUBMED   22198343
  REMARK    GeneRIF: Disruption of electron flux from fuel substrates to redox
            components due to NNT suppression induces not only mitochondrial
            dysfunction but also cellular disorders through redox-sensitive
            signaling.
REFERENCE   5  (bases 1 to 4149)
  AUTHORS   Houtkooper RH, Canto C, Wanders RJ and Auwerx J.
  TITLE     The secret life of NAD+: an old metabolite controlling new
            metabolic signaling pathways
  JOURNAL   Endocr Rev 31 (2), 194-223 (2010)
   PUBMED   20007326
  REMARK    Review article
REFERENCE   6  (bases 1 to 4149)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 4149)
  AUTHORS   Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl
            E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES and
            Mann M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 4149)
  AUTHORS   Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA and
            Martinou JC.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   9  (bases 1 to 4149)
  AUTHORS   White SA, Peake SJ, McSweeney S, Leonard G, Cotton NP and Jackson
            JB.
  TITLE     The high-resolution structure of the NADP(H)-binding component
            (dIII) of proton-translocating transhydrogenase from human heart
            mitochondria
  JOURNAL   Structure 8 (1), 1-12 (2000)
   PUBMED   10673423
REFERENCE   10 (bases 1 to 4149)
  AUTHORS   Arkblad EL, Helou K, Levan G and Rydstrom J.
  TITLE     Mapping of the rat and mouse nicotinamide nucleotide
            transhydrogenase gene
  JOURNAL   Mamm Genome 8 (9), 703 (1997)
   PUBMED   9271681
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC091271.1.
            
            On Apr 20, 2005 this sequence version replaced XM_227084.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091271.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN11044071 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q15"
     gene            1..4149
                     /gene="Nnt"
                     /note="nicotinamide nucleotide transhydrogenase"
                     /db_xref="GeneID:310378"
                     /db_xref="RGD:1587346"
     exon            1..91
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            92..294
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..3404
                     /gene="Nnt"
                     /EC_number="1.6.1.2"
                     /note="Nicotinamide nucleotide transhydrogenase (NAD(P)+
                     transhydrogenase)"
                     /codon_start=1
                     /product="NAD(P) transhydrogenase, mitochondrial"
                     /protein_id="NP_001013175.1"
                     /db_xref="GeneID:310378"
                     /db_xref="RGD:1587346"
                     /translation="MAHLLKTVVTGCLCPSVSNLGSCKVLPGKKDFLRTFRTHQALWC
                     KSPAKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFP
                     DDLYRAAGAQIQGTKEVLASDLVVKVRAPMVNPTLGAHEADFLKPSGTLISFIYPAQN
                     PDLLNKLSERKTTVLAMDQVPRVTIAQGYDALSSMANISGYKAVVLAANHFGRFFTGQ
                     ITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAVVRGFDTRAAALEQFKSLGAEPLEV
                     DLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPVLFSKEMI
                     ESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNI
                     TKLLKAISPDKDNFHFEVKDDFDFGTMSHVIRGTVVMKDGNVIFPAPTPKNIPKEAPA
                     KQKTVAELEAEKAGTVSMYTKTLRTASVYSAGLTGMLGLGIVAPNLAFSQMVTTFGLA
                     GIIGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHFYPSTTSQSLAALATFI
                     SSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPGGTFVGGYLAALYGGYNIEEIMY
                     LGSGLCCVGALGGLSTQGTARLGNALGMIGVAGGLAATLGGLKPDPQLLAQMSGAMAM
                     GGTIGLAIAKRIQISDLPQLVAAFHSLVGLAAVLTCMAEYIVEYPHFAMDATSNFTKI
                     VAYIGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHALNAGLLAASVGGIIPFMAD
                     PSFTTGIMCLGSVSALSTLMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNN
                     LLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINL
                     DNAVEMIREANSIVITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQ
                     LNVLLAEAGVPYDIVLEMDEINSDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVL
                     EVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK"
     exon            295..524
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            525..742
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            743..830
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            831..919
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1107
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1241
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1433
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1587
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1749
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1860
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..2006
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2202
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2436
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2597
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2777
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            2778..2937
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            2938..3019
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            3020..3138
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3254
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
     exon            3255..4125
                     /gene="Nnt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacagcgccg cgggccaagc ccgggtctgc caacgcggcg tggcgtttgc tcgcagcccg
       61 gggtcaccat ctgtcccgcc tgcctgtcca gtaaaccacc ttcgaggaat tggggatttg
      121 aaaagttggg agcgcaaagc aacatggcac atctactgaa aacggtggtg actggctgtt
      181 tatgtccttc tgttagcaat ttggggtcct gtaaggttct acctgggaag aaagactttt
      241 tacgaacatt tcgtacccac caggcactgt ggtgtaaatc cccggcaaaa ccaggaattc
      301 catataagca gttgacagtt ggggtcccca aggagatttt ccaaaatgag aagcgagttg
      361 ccttgtctcc tgctggtgtc caggccctgg tcaagcaggg ttttaacgtt gttgtggaat
      421 caggagcagg cgaagcttcc aaattcccag atgatctcta cagagcagca ggggcccaga
      481 tccaaggaac gaaggaagtt ctggcttctg atctggtggt caaagtgagg gcccccatgg
      541 tgaacccgac gttaggtgct catgaagctg actttttaaa gccctcagga actctaatta
      601 gcttcattta cccagcccaa aatccagact tgctaaataa actctccgaa agaaaaacta
      661 cagttttggc gatggaccag gttccgaggg tgacaatagc tcagggctat gatgctctca
      721 gctccatggc caacatttct ggttataagg ctgttgtttt agcagccaat cattttggaa
      781 ggtttttcac tggtcagatc acagctgctg gaaaagtccc cccagcgaag atcctgatag
      841 ttggtggtgg cgttgccggg ctcgcttctg caggggcagc aaagtcaatg ggcgcagttg
      901 tccgaggatt tgatacaaga gctgccgcct tggaacagtt caaatccctt ggtgctgaac
      961 ccttggaggt ggacttgaag gagtctggtg agggccaagg aggatacgcg aaggagatgt
     1021 ccaaagagtt tattgaggcg gaaatgaagc tctttgctca gcaatgcaag gaagtggaca
     1081 tccttatcag cacagctctg attccaggta agaaagctcc tgttctattt agtaaagaaa
     1141 tgatcgagtc catgaaggaa ggttcagtag ttgtggattt agctgctgag gccggcggaa
     1201 actttgaaac gactaaaccg ggagaacttt acgttcataa gggaatcact catattggct
     1261 acacagacct ccccagccgc atggccactc aggctagcac tctgtactcc aacaacatca
     1321 ccaagctcct gaaggccatc agccccgaca aggacaattt tcactttgaa gtgaaggatg
     1381 actttgactt tggtacaatg agtcacgtca ttcgaggaac cgtggtgatg aaggatggca
     1441 atgtgatttt cccagctccc acaccaaaaa atattcccaa agaagcccca gccaaacaga
     1501 agaccgtggc tgagctggaa gcagagaagg caggcactgt ctcaatgtac acgaagacac
     1561 tgaggacagc atccgtgtat tcagcaggtc tcaccgggat gctaggtttg ggcatcgtgg
     1621 cccccaatct agccttttct cagatggtga ccacctttgg cctggcgggc attattggtt
     1681 accacactgt ctggggagtg acccctgctc tccactcacc actgatgtct gtgaccaatg
     1741 ctatctcagg tttgactgca gttggtgggc tggcgttgat gggagggcat ttttatccct
     1801 ccacaacttc ccagagtctt gctgctcttg ctacattcat atcgtcagtc aacattgcag
     1861 gtggttttct ggtgactcag agaatgttgg acatgttcaa gcgacccacg gaccctccag
     1921 aatacaatta cctgtatctg ctccctggcg gcaccttcgt ggggggatat ttagcagctc
     1981 tctacggtgg ttataacatc gaagaaatca tgtacctggg ctcaggtttg tgctgtgttg
     2041 gggccctggg cggcctttcc actcagggaa cagctcgact cggcaatgcc ttgggcatga
     2101 ttggggttgc aggaggcctg gcagctaccc ttggaggcct gaagccggac ccacagctgt
     2161 tagctcagat gtccggagca atggcgatgg gcggcaccat cggactggcg attgcaaagc
     2221 ggatccagat ctccgacctg cctcaactgg ttgctgcttt ccacagttta gtgggtctgg
     2281 cggcagtgct cacatgcatg gccgagtaca tcgtggagta tccacatttc gcaatggatg
     2341 ccacttcaaa tttcaccaag attgtggcct acattggcac ttacatcggc ggagtcacct
     2401 ttagtgggtc tctggttgcc tacggaaaat tacagggtat tctgaagtca gcccctctgc
     2461 tgctccctgg aaggcacgcg ctcaatgcag gcttgctggc tgctagcgtg gggggaataa
     2521 tcccatttat ggctgaccct agttttacca caggcatcat gtgtctgggc tctgtgtccg
     2581 cactctctac tctgatgggt gtgaccttga cagctgccat tggcggtgct gacatgcctg
     2641 ttgttatcac tgtgctaaac agctactccg gctgggctct gtgtgcggag ggcttcctgc
     2701 tcaacaacaa cctgttgacc attgtgggag cactcatcgg ctcctcgggc gccatcttgt
     2761 cgtacatcat gtgtgtggca atgaaccgtt ccctggcaaa tgtaattctc ggaggctatg
     2821 gtacgacttc aacagctggt ggaaaaccca tggaaatttc cggcacacat acagaaatca
     2881 acctggacaa tgcagtcgag atgatccggg aagctaacag cattgttatt actccaggat
     2941 atgggctctg tgcagctaaa gctcagtacc ccattgccga tttggtgaag atgcttaccg
     3001 aacaaggcaa gaaagtcagg tttggaatcc acccagttgc gggccgaatg cctggtcagc
     3061 ttaatgtgct cctggccgag gcaggagtgc cctatgatat tgtgctagaa atggatgaga
     3121 tcaacagtga tttcccagat accgatctgg ttcttgtcat tggagctaat gacaccgtga
     3181 attcagcggc tcaggaagac cccaattcta ttattgcagg catgccagtc ctcgaggtct
     3241 ggaagtccaa acaggtcatt gtcatgaaga ggtcccttgg tgttggctac gctgcagtgg
     3301 acaatccaat cttctacaaa cctaacacag ccatgcttct gggggacgcc aagaagacat
     3361 gtgatgctct gcaggccaaa gttcgagaat cctatcagaa gtgaatattg acaatcaagc
     3421 cagtgccttc ggttgcagga acagagaatg agacatttat agatgagcaa agctctcaag
     3481 gcccgaaaga agcctcagaa aacaatggag attttcaaga tcagcaaccc ttgattctca
     3541 aaaagcatcg ggagttctca tcatctactt gtttatcatt gccattgcca aaaagcagag
     3601 tattttgtca gcagtgagag agtggcctca ctgtagatgc aatcccattg gattttgcaa
     3661 ccccctttcg ggagctagaa atgagctcag aagttgaatg ttcaagattt caggatatgt
     3721 tttggtctga agaatattat gctgtttgat catcacaggc acatgcattt agtgttttat
     3781 ttttccacca aatgtgacaa attttgaaac tttgatcaac tcctgacctg ttctcttgca
     3841 ttttagccgt agtctggatt gatcatgcca attccatatc aggtttttag ccttcactct
     3901 agactctaga ttccaaagat ctattttctc acttctattt taatatgtag aagatctaga
     3961 tagtttaagt aataacttaa aaggaataag ttgtattttt aaaagtccca tgccaattgg
     4021 tctctttaat ttcaatcaat taagtgctct tgaatggtct gtattaagtg ctcttgaatg
     4081 gaatgcaacc atttgagtaa taaatgagtt catcatgttc tcaaaaaaaa aaaaaaaaaa
     4141 aaaaaaaaa
//
