LOCUS       NM_001309432            3441 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus NLR family, CARD domain containing 4 (Nlrc4),
            mRNA.
ACCESSION   NM_001309432
VERSION     NM_001309432.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Gan,H., Zhang,L., Chen,H., Xiao,H., Wang,L., Zhai,X., Jiang,N.,
            Liang,P., Zheng,S. and Zhao,J.
  TITLE     The pivotal role of the NLRC4 inflammasome in neuroinflammation
            after intracerebral hemorrhage in rats
  JOURNAL   Exp Mol Med 53 (11), 1807-1818 (2021)
   PUBMED   34848837
  REMARK    GeneRIF: The pivotal role of the NLRC4 inflammasome in
            neuroinflammation after intracerebral hemorrhage in rats.
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Habib,P., Harms,J., Zendedel,A., Beyer,C. and Slowik,A.
  TITLE     Gonadal Hormones E2 and P Mitigate Cerebral Ischemia-Induced
            Upregulation of the AIM2 and NLRC4 Inflammasomes in Rats
  JOURNAL   Int J Mol Sci 21 (13), 4795 (2020)
   PUBMED   32645874
  REMARK    GeneRIF: Gonadal Hormones E2 and P Mitigate Cerebral
            Ischemia-Induced Upregulation of the AIM2 and NLRC4 Inflammasomes
            in Rats.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3441)
  AUTHORS   Diebolder,C.A., Halff,E.F., Koster,A.J., Huizinga,E.G. and
            Koning,R.I.
  TITLE     Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome:
            Implications for NLR Activation
  JOURNAL   Structure 23 (12), 2349-2357 (2015)
   PUBMED   26585513
REFERENCE   4  (bases 1 to 3441)
  AUTHORS   Hu,Z., Zhou,Q., Zhang,C., Fan,S., Cheng,W., Zhao,Y., Shao,F.,
            Wang,H.W., Sui,S.F. and Chai,J.
  TITLE     Structural and biochemical basis for induced self-propagation of
            NLRC4
  JOURNAL   Science 350 (6259), 399-404 (2015)
   PUBMED   26449475
REFERENCE   5  (bases 1 to 3441)
  AUTHORS   Zhang,L., Chen,S., Ruan,J., Wu,J., Tong,A.B., Yin,Q., Li,Y.,
            David,L., Lu,A., Wang,W.L., Marks,C., Ouyang,Q., Zhang,X., Mao,Y.
            and Wu,H.
  TITLE     Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals
            nucleated polymerization
  JOURNAL   Science 350 (6259), 404-409 (2015)
   PUBMED   26449474
REFERENCE   6  (bases 1 to 3441)
  AUTHORS   Damiano,J.S., Oliveira,V., Welsh,K. and Reed,J.C.
  TITLE     Heterotypic interactions among NACHT domains: implications for
            regulation of innate immune responses
  JOURNAL   Biochem J 381 (Pt 1), 213-219 (2004)
   PUBMED   15107016
REFERENCE   7  (bases 1 to 3441)
  AUTHORS   Agostini,L., Martinon,F., Burns,K., McDermott,M.F., Hawkins,P.N.
            and Tschopp,J.
  TITLE     NALP3 forms an IL-1beta-processing inflammasome with increased
            activity in Muckle-Wells autoinflammatory disorder
  JOURNAL   Immunity 20 (3), 319-325 (2004)
   PUBMED   15030775
REFERENCE   8  (bases 1 to 3441)
  AUTHORS   Masumoto,J., Dowds,T.A., Schaner,P., Chen,F.F., Ogura,Y., Li,M.,
            Zhu,L., Katsuyama,T., Sagara,J., Taniguchi,S., Gumucio,D.L.,
            Nunez,G. and Inohara,N.
  TITLE     ASC is an activating adaptor for NF-kappa B and caspase-8-dependent
            apoptosis
  JOURNAL   Biochem Biophys Res Commun 303 (1), 69-73 (2003)
   PUBMED   12646168
REFERENCE   9  (bases 1 to 3441)
  AUTHORS   Poyet,J.L., Srinivasula,S.M., Tnani,M., Razmara,M.,
            Fernandes-Alnemri,T. and Alnemri,E.S.
  TITLE     Identification of Ipaf, a human caspase-1-activating protein
            related to Apaf-1
  JOURNAL   J Biol Chem 276 (30), 28309-28313 (2001)
   PUBMED   11390368
REFERENCE   10 (bases 1 to 3441)
  AUTHORS   Geddes,B.J., Wang,L., Huang,W.J., Lavellee,M., Manji,G.A.,
            Brown,M., Jurman,M., Cao,J., Morgenstern,J., Merriam,S.,
            Glucksmann,M.A., DiStefano,P.S. and Bertin,J.
  TITLE     Human CARD12 is a novel CED4/Apaf-1 family member that induces
            apoptosis
  JOURNAL   Biochem Biophys Res Commun 284 (1), 77-82 (2001)
   PUBMED   11374873
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.21631.1,
                                           SRR26643288.25560.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               JAXUCZ010000006.1  26747139-26747243
            106-366             JAXUCZ010000006.1  26751063-26751323
            367-2358            JAXUCZ010000006.1  26752203-26754194
            2359-2451           JAXUCZ010000006.1  26755608-26755700
            2452-2622           JAXUCZ010000006.1  26759219-26759389
            2623-2715           JAXUCZ010000006.1  26760033-26760125
            2716-2883           JAXUCZ010000006.1  26762709-26762876
            2884-3441           JAXUCZ010000006.1  26769564-26770121
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q13"
     gene            1..3441
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /note="NLR family, CARD domain containing 4"
                     /db_xref="GeneID:298784"
                     /db_xref="RGD:1309831"
     exon            1..105
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..3176
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /note="caspase recruitment domain family, member 12; ice
                     protease-activating factor"
                     /codon_start=1
                     /product="NLR family CARD domain-containing protein 4"
                     /protein_id="NP_001296361.1"
                     /db_xref="GeneID:298784"
                     /db_xref="RGD:1309831"
                     /translation="MNFIKENSQALIQRMGIVVIKQICDDLFALNVLNGEEVAIICSH
                     RVEQDAARDIVHMILKKGSAACNLFLKSLENWNYPVYQDLTGHSLFHQNLEEDLDVLA
                     QSLKDLYNSPVFKNFFPLGEDIDIIFNLQITFTEPVLWRKDHRHHRVEQMTLGSLLEA
                     LKSPCLIEGESGKGKSTLLQKIAMLWASGMCPALNQFKLVFFIRLSSARGGLFETLYD
                     QLVNIPDSISKPTFRALLLKLHKKVLFLLDAYNEFHPQNCPEIEALVKENHRFKNMVI
                     VTTTTECLRHIRHVGALTVEVGDMTEDSARVLIREVLINELAEGLLFQMQESRCLRNL
                     MRTPLFVVITCAIQMGSEEFQAHTQTMLFQTFYDLLIQKNRRRHSGGTSGDFVRSLDY
                     CGDLALEGVFSHKFDFELEDVCSMNEDVLVRTGLLCKYTAQRLRPTYKFFHKSFQEYT
                     AGRRLSSLLKSREPEEVSKGNSYLKKMVSISDITSLYGNLLLYTCGSSTEATRAIMRH
                     LAMVCEHGSLQGLSVTKRPLWRQESIQNLRNTTEQDVLKAINVNSFVECGINLFSESI
                     SKSELSQEFEAFFQGKSLYINSENIPDYLFDFFKYLPNCVSALDFVKLDFYGRATVSQ
                     DKTGENSSGVHTEGPSTYIPSRAVSLFFNWMQKFKTLEVTLRDISKLNKQDIKYLGKI
                     FSSASNLKLYIKRCAAVAGRLSSVLRTCKNIHSLMVEASPLTTEDEQYITSVTDLQNL
                     SIHDLHTQRLPGGLADSLGNLKNLLKLILDDIRLNEEDAKSLAEGLRNLKKMRLLHLT
                     RLSDMGEGMDYIVKSLSEEPCDLQEMKLVDCCLTANSLKILAQNLHNLVKLSVLDMSE
                     NYLEKAGSEALQGLIGRLGVLEQLSALMLPWCWDAYISLPNLLKQLEGTPGLVKLGLK
                     NWRLRDEEIRSFGEFLEMNPLRDLQQLDLAGHGVSSDGWLSFMDVFENLKQLVFFDFG
                     TEEFLPDAALVRKLGQVLSKLTLLQEARLTGWELDDYDISVIKGTFKLVTA"
     exon            106..366
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     exon            367..2358
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2451
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2622
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2715
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2883
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
     exon            2884..3441
                     /gene="Nlrc4"
                     /gene_synonym="Card12; Ipaf"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gatagcctta ccttacaaca ttatgatcgt ctttggcctt cctgtttgga aaagaaatac
       61 cttctactga gatcaagaaa agtgggacgg tttgactcac cacaatgaac tttataaagg
      121 aaaacagcca agcccttatt cagaggatgg gcatagtggt tataaagcaa atctgtgatg
      181 acctatttgc attgaacgtt ctcaacgggg aagaagttgc catcatttgc agtcatcggg
      241 tggagcagga cgctgcacga gacatcgtcc atatgatttt gaagaagggc tcagcggcct
      301 gcaacctctt tcttaagagt cttgaaaact ggaactatcc tgtgtatcag gacttaactg
      361 gacacagtct ttttcatcag aacttggaag aagacttgga tgttctggcc cagagtctaa
      421 aagacttata caacagccct gtttttaaga acttctttcc tctgggtgag gatatcgaca
      481 tcattttcaa tctgcagatc accttcacag aacctgtctt gtggaggaag gaccatcgtc
      541 atcaccgagt agagcagatg accctgggca gcctgctgga ggctctgaag agtccctgcc
      601 tcattgaagg ggagtctggc aaagggaagt ccaccctgct gcaaaaaatt gccatgctct
      661 gggcctctgg gatgtgccca gctctgaacc agttcaaatt ggtcttcttc atccgcctga
      721 gcagtgctag gggtggcctg tttgaaacat tgtatgatca gctcgtgaac atacctgact
      781 ccatcagcaa accaaccttc agggctctgc tgctgaagtt acacaagaaa gtcctctttc
      841 tcctcgatgc ttacaatgaa ttccatcccc agaactgccc agaaatcgag gccctggtaa
      901 aggaaaacca tcgtttcaag aacatggtca ttgtcaccac caccacggag tgcctgaggc
      961 acatcagaca cgttggcgcc ctgactgtgg aggtgggaga tatgaccgaa gacagcgccc
     1021 gagttctcat ccgggaagtg ctgataaatg aactggctga aggcttgttg ttccagatgc
     1081 aggagtccag gtgcttgaga aatctgatga ggacccctct cttcgtggtg ataacctgtg
     1141 ccatccagat gggcagtgag gaattccaag ctcacactca aaccatgctc ttccaaacct
     1201 tctacgacct cctgatacag aaaaacaggc gcagacacag tggaggaact tcaggtgatt
     1261 ttgtcaggag cctagactac tgtggagacc tggccctgga aggtgtgttc tcccacaagt
     1321 ttgatttcga acttgaggat gtgtgcagca tgaatgagga cgtcctggtg aggacggggc
     1381 tcctctgtaa gtacacggct cagaggctga ggcccacgta taaattcttt cataaatcct
     1441 ttcaggagta cacagcaggt cggagactca gcagtttgtt gaagtccaga gagccagagg
     1501 aggtgagcaa ggggaatagc tacttgaaga aaatggtttc catctccgac atcacatccc
     1561 tgtatggtaa tctgctcctg tacacttgtg ggtcatccac agaagcaacc agggccatca
     1621 tgaggcacct tgcaatggta tgtgagcatg gcagcctaca aggactttct gtcaccaagc
     1681 ggcctctttg gagacaggag tcaatacaaa acctgagaaa caccactgag caagatgttc
     1741 tgaaagccat caacgtaaat tcctttgtag agtgtggtat caatttattc tcggagagta
     1801 tatctaaatc agagctgagc caagaatttg aagctttctt ccaagggaaa agtttataca
     1861 tcaactccga gaacatccct gattacttat tcgacttctt taaatacttg cctaattgtg
     1921 taagcgcact ggacttcgtc aagctggatt tctatggaag agcgacagtg tcccaggaca
     1981 agacgggaga gaacagctct ggagtccaca cggaagggcc ctcgacctac attcccagta
     2041 gggctgtgtc tttgttcttc aattggatgc agaaattcaa gactctagag gtcacactcc
     2101 gagatattag caagttgaat aaacaagata tcaaatacct ggggaagata ttcagctccg
     2161 ccagcaacct caagttgtac atcaagaggt gcgcagccgt ggccggaagg ctcagctcag
     2221 tcctcagaac ctgcaagaac atccactccc tcatggtgga agctagtccc ctcaccacgg
     2281 aggatgagca gtacatcacg tctgtgacag acctccagaa cttaagcatt cacgacctcc
     2341 acactcaacg gctgccaggt ggtctggctg acagcttggg taatctgaag aacctcctga
     2401 agctcatcct ggatgacatt aggctgaacg aggaagatgc taaaagccta gcggaaggcc
     2461 tgcgaaacct gaagaagatg cgtttacttc atttgactcg tttgtctgac atgggggagg
     2521 ggatggacta catagtcaag tctctctcgg aagaaccctg tgatctacaa gagatgaagt
     2581 tggtggactg ctgtctgacc gcaaattctc tgaaaattct agcacagaat cttcacaatt
     2641 tggtcaagct gagcgttctt gatatgtcag aaaattatct ggaaaaggcc ggaagtgaag
     2701 ctctacaggg actgatcggc aggcttggcg tgctagaaca gctaagcgcg ttgatgctgc
     2761 cttggtgctg ggatgcgtac atcagtctgc ccaatctgct gaagcaactg gaggggaccc
     2821 cagggcttgt caaacttgga ttgaaaaact ggaggctcag agacgaagag attagaagtt
     2881 tcggtgaatt tttggagatg aatcctctga gagacttgca gcagttggat ttagcaggac
     2941 acggtgtgag cagtgatgga tggctttctt tcatggatgt gtttgagaat cttaagcagt
     3001 tggtattttt tgacttcggc actgaagagt tcttaccaga cgcagcgctg gtgaggaaac
     3061 tcggtcaagt gttatctaag ctaactctgc tgcaagaggc aaggctcaca ggctgggagc
     3121 ttgatgacta tgacataagt gttattaaag gaacctttaa actagtgact gcttgatgta
     3181 ccccaagcca ccaaatactc cggggactct gccactttca gcggatcatt agaaaccacg
     3241 cagaagctcg gctgcagagc tggcttaggg gttaagagca tttgctgctc ttccagggga
     3301 cataggtttg attcgcagca cccacaaggt ggctcacaac cgtctgtaac tccagttcca
     3361 ggggatctaa tgtcttctga tctctatggg taccctgcaa gcaaaacact cgtatacatt
     3421 aaataaaatg ttaaaatact t
//
