LOCUS       NM_053970               2705 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Rattus norvegicus neurolysin (Nln), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_053970
VERSION     NM_053970.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Hines,C.S., Ray,K., Schmidt,J.J., Xiong,F., Feenstra,R.W.,
            Pras-Raves,M., de Moes,J.P., Lange,J.H., Melikishvili,M.,
            Fried,M.G., Mortenson,P., Charlton,M., Patel,Y., Courtney,S.M.,
            Kruse,C.G. and Rodgers,D.W.
  TITLE     Allosteric inhibition of the neuropeptidase neurolysin
  JOURNAL   J Biol Chem 289 (51), 35605-35619 (2014)
   PUBMED   25378390
  REMARK    GeneRIF: Data indicate that the inhibitor binds away from the
            catalytic site of neuropeptidase neurolysin.
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Cavalcanti,D.M., Castro,L.M., Rosa Neto,J.C., Seelaender,M.,
            Neves,R.X., Oliveira,V., Forti,F.L., Iwai,L.K., Gozzo,F.C.,
            Todiras,M., Schadock,I., Barros,C.C., Bader,M. and Ferro,E.S.
  TITLE     Neurolysin knockout mice generation and initial phenotype
            characterization
  JOURNAL   J Biol Chem 289 (22), 15426-15440 (2014)
   PUBMED   24719317
REFERENCE   3  (bases 1 to 2705)
  AUTHORS   Guo,L., Zheng,Z., Ai,J., Huang,B. and Li,X.A.
  TITLE     Hepatic scavenger receptor BI protects against
            polymicrobial-induced sepsis through promoting LPS clearance in
            mice
  JOURNAL   J Biol Chem 289 (21), 14666-14673 (2014)
   PUBMED   24719333
REFERENCE   4  (bases 1 to 2705)
  AUTHORS   Swindle,J.D., Santos,K.L. and Speth,R.C.
  TITLE     Pharmacological characterization of a novel non-AT1, non-AT2
            angiotensin binding site identified as neurolysin
  JOURNAL   Endocrine 44 (2), 525-531 (2013)
   PUBMED   23412923
  REMARK    GeneRIF: Neurolysin was identified as a non-AT1 and non-AT2 binding
            site for Angiotensin II, suggesting that neurolysin may
            significantly affect the activity of the renin-angiotensin system.
REFERENCE   5  (bases 1 to 2705)
  AUTHORS   Wangler,N.J., Santos,K.L., Schadock,I., Hagen,F.K., Escher,E.,
            Bader,M., Speth,R.C. and Karamyan,V.T.
  TITLE     Identification of membrane-bound variant of metalloendopeptidase
            neurolysin (EC 3.4.24.16) as the non-angiotensin type 1 (non-AT1),
            non-AT2 angiotensin binding site
  JOURNAL   J Biol Chem 287 (1), 114-122 (2012)
   PUBMED   22039052
REFERENCE   6  (bases 1 to 2705)
  AUTHORS   Ray,K., Hines,C.S. and Rodgers,D.W.
  TITLE     Mapping sequence differences between thimet oligopeptidase and
            neurolysin implicates key residues in substrate recognition
  JOURNAL   Protein Sci 11 (9), 2237-2246 (2002)
   PUBMED   12192079
  REMARK    GeneRIF: Data report mapping of the positions of the 224 surface
            residues that differ in sequence between thimet oligopeptidase and
            neurolysin onto the surface of the neurolysin crystal structure.
REFERENCE   7  (bases 1 to 2705)
  AUTHORS   Brown,C.K., Madauss,K., Lian,W., Beck,M.R., Tolbert,W.D. and
            Rodgers,D.W.
  TITLE     Structure of neurolysin reveals a deep channel that limits
            substrate access
  JOURNAL   Proc Natl Acad Sci U S A 98 (6), 3127-3132 (2001)
   PUBMED   11248043
REFERENCE   8  (bases 1 to 2705)
  AUTHORS   Kato,A., Sugiura,N., Saruta,Y., Hosoiri,T., Yasue,H. and Hirose,S.
  TITLE     Targeting of endopeptidase 24.16 to different subcellular
            compartments by alternative promoter usage
  JOURNAL   J Biol Chem 272 (24), 15313-15322 (1997)
   PUBMED   9182559
REFERENCE   9  (bases 1 to 2705)
  AUTHORS   Dauch,P., Vincent,J.P. and Checler,F.
  TITLE     Molecular cloning and expression of rat brain endopeptidase
            3.4.24.16
  JOURNAL   J Biol Chem 270 (45), 27266-27271 (1995)
   PUBMED   7592986
REFERENCE   10 (bases 1 to 2705)
  AUTHORS   Serizawa,A., Dando,P.M. and Barrett,A.J.
  TITLE     Characterization of a mitochondrial metallopeptidase reveals
            neurolysin as a homologue of thimet oligopeptidase
  JOURNAL   J Biol Chem 270 (5), 2092-2098 (1995)
   PUBMED   7836437
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC072687.1.
            
            On Jul 8, 2004 this sequence version replaced NM_053970.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC072687.1, X87157.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q13"
     gene            1..2705
                     /gene="Nln"
                     /note="neurolysin"
                     /db_xref="GeneID:117041"
                     /db_xref="RGD:621518"
     exon            1..76
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..2150
                     /gene="Nln"
                     /EC_number="3.4.24.16"
                     /note="neurolysin, mitochondrial; MEP; microsomal
                     endopeptidase; mitochondrial oligopeptidase M; neurolysin
                     (metallopeptidase M3 family); neurotensin endopeptidase"
                     /codon_start=1
                     /product="neurolysin, mitochondrial precursor"
                     /protein_id="NP_446422.2"
                     /db_xref="GeneID:117041"
                     /db_xref="RGD:621518"
                     /translation="MITLCLSTLRGLHRAGGSRLQLTMTLGKELASPLQAMSSYTAAG
                     RNVLRWDLSPEQIKTRTEQLIAQTKQVYDTVGTIALKEVTYENCLQVLADIEVTYIVE
                     RTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKP
                     EARRYLEKSIKMGKRNGLHLSEHIRNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKA
                     ELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKQEN
                     TAILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSRVAAFLDDLSQKLKPLGEAER
                     EFILSLKKKECEERGFEYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEG
                     LLSIYQELLGLSFEQVPDAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAA
                     CFGLQPGCLLPDGSRMMSVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICA
                     QTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRKLSKHYKDGHPITDELLEKLVAS
                     RLVNTGLLTLRQIVLSKVDQSLHTNATLDAASEYAKYCTEILGVAATPGTNMPATFGH
                     LAGGYDGQYYGYLWSEVFSMDMFHSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDML
                     QNFLQREPNQKAFLMSRGLNGS"
     exon            77..336
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            337..485
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            486..593
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            594..696
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            697..857
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            858..993
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1360
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1562
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1749
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1878
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2015
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2680
                     /gene="Nln"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gacgcgccag ctgctgccgg cctcccggcg tgcccatgat caccctgtgc ctttcgactc
       61 tgcgaggcct ccacagagct ggtggctcca ggcttcagct gacaatgaca ctgggaaagg
      121 agctggcttc tcctcttcaa gctatgtctt cctacactgc ggctggcagg aatgttctca
      181 gatgggacct ttctccagag cagatcaaga cgaggacaga gcagctcata gcacagacca
      241 agcaggtgta cgacaccgtg gggacaatcg ccctgaaaga agtaacttat gagaactgtc
      301 tgcaggtgct ggctgacata gaagtgacgt acatagtgga aaggaccatg ctggacttcc
      361 ctcagcacgt gtcatctgac agagaagtgc gggctgcaag cacagaagca gacaaaaggc
      421 tttctcgttt tgatattgag atgagcatga gagaagatgt gtttcagaga attgttcatt
      481 tacaagaaac atgcgatttg gagaagataa agcctgaagc caggcgatac ttggaaaagt
      541 caattaaaat gggaaaaagg aatggactcc atctttcaga acacataaga aatgaaatca
      601 aatcaatgaa gaagagaatg agtgagctat gcattgactt caacaagaac ctcaatgagg
      661 acgacacttc ccttgtattc tccaaggctg aacttggtgc tcttcctgat gacttcattg
      721 acagtttaga aaaaacagat gaagacaagt acaaagttac cttaaagtat cctcactact
      781 tccctgtcat gaagaaatgc tgtgtccctg aaactcgaag gaagatggaa atggcttttc
      841 ataccaggtg caaacaggaa aacaccgcaa ttctgcaaca gctcctcccg ctgcgagctc
      901 aagtggccaa gctgctgggc tataacaccc atgctgactt tgtccttgaa ctgaacactg
      961 cgaagagcac cagccgcgtg gccgcctttc tagatgattt aagccagaaa ttgaaaccct
     1021 tgggtgaggc agaacgggag tttattttga gcctgaagaa gaaggaatgt gaagagcgag
     1081 gttttgaata cgacgggaag atcaatgcat gggacctcca ttactacatg acacagacag
     1141 aggagctcaa gtactcggtg gaccaggagt cccttaagga gtacttcccc attgaggtgg
     1201 tcacggaagg cttgctaagc atctaccagg agctgctggg actttcattt gaacaagtgc
     1261 ccgatgctca tgtttggaat aagagcgttt cactttacac tgtgaaggat aaagctacag
     1321 gagaggtcct ggggcagttc tacctggacc tctatccaag ggaaggaaaa tacaaccatg
     1381 cagcctgctt cggtctccag ccaggctgcc ttctccctga tggcagtcgg atgatgtctg
     1441 tggccgccct ggtggtcaac ttctctcagc ctgtagcagg tcggccctct ctcctgagac
     1501 atgatgaagt gcggacttac ttccacgagt tcggtcacgt catgcatcag atctgtgcgc
     1561 agactgactt tgcacgattc agtggaacaa acgtggaaac tgactttgta gaggtgccat
     1621 cacaaatgct tgaaaactgg gtgtgggacg ttgattccct gcgaaaactg tcaaagcatt
     1681 ataaagatgg acaccctatc acagacgagc tgctcgagaa gcttgttgct tctaggctgg
     1741 tcaacacagg tcttctgacg ctgcgccaaa ttgttttgag caaagttgac caatctctcc
     1801 ataccaatgc gactctggat gccgcgagcg aatacgctaa atactgcaca gaaattctgg
     1861 gagttgcagc tacgccaggc acaaatatgc cagctacttt tggacacttg gcaggagggt
     1921 atgatggcca atattatgga tatctttgga gtgaagtgtt ttccatggac atgtttcaca
     1981 gctgttttaa aaaagagggg atcatgaatc cagaggttgg aatgaaatac agaaacttaa
     2041 tcctgaagcc tggggggtcc ctggacggca tggacatgct ccagaatttc ttgcaacgtg
     2101 agccaaacca aaaagcattc ctaatgagtc gaggcctgaa tggttcgtaa gccagggatc
     2161 ctgggagttg tccatgtctg gaggacaagt caacactgtc atgtggtgcc ggcctggaga
     2221 ctgaagagag ttttacaaaa gaaaacttag atttctattg atttgctttt gttttttaat
     2281 tttaaaaatt atacagatgt aaatggaatt ataaatactg tgacctaagg aaagaccaac
     2341 taaaaataat tgtactataa agtttcataa aaatggattt gatttctttt gataaaagtt
     2401 tcatatgaat gtaatttgat tttttttaca gttataatcc agataatata atgtaagagg
     2461 gctaagacat tttaaattga atcacataca tgatataatt tgatccttct tatatcttga
     2521 agttttatac ttgcgatctc tggactgata aatgaatcac cacattctct ctggtaaata
     2581 tttcctccga gccctgtgtc aaccttgacc ctctgcctca gagctaggcg acagtctgcc
     2641 tgcgtgtgtc aaggccagca caacatgttt cagtagtgtt aaaaaaaaaa aaaaaaaaaa
     2701 aaaaa
//
