LOCUS       NM_001034126            1054 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Rattus norvegicus nitrilase family, member 2 (Nit2), mRNA.
ACCESSION   NM_001034126 XM_213637
VERSION     NM_001034126.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Chien,C.H., Gao,Q.Z., Cooper,A.J., Lyu,J.H. and Sheu,S.Y.
  TITLE     Structural insights into the catalytic active site and activity of
            human Nit2/omega-amidase: kinetic assay and molecular dynamics
            simulation
  JOURNAL   J Biol Chem 287 (31), 25715-25726 (2012)
   PUBMED   22674578
REFERENCE   3  (bases 1 to 1054)
  AUTHORS   Krasnikov,B.F., Chien,C.H., Nostramo,R., Pinto,J.T., Nieves,E.,
            Callaway,M., Sun,J., Huebner,K. and Cooper,A.J.
  TITLE     Identification of the putative tumor suppressor Nit2 as
            omega-amidase, an enzyme metabolically linked to glutamine and
            asparagine transamination
  JOURNAL   Biochimie 91 (9), 1072-1080 (2009)
   PUBMED   19595734
  REMARK    GeneRIF: Data report that rat liver Nit2 is omega-amidase and in
            vivo amidase substrates are alpha-ketoglutaramate and
            alpha-ketosuccinamate, generated by transamination of glutamine and
            asparagine, respectively.
REFERENCE   4  (bases 1 to 1054)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   5  (bases 1 to 1054)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC100637.1.
            
            On Oct 14, 2005 this sequence version replaced XM_213637.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC100637.1, FQ219183.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q12"
     gene            1..1054
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /note="nitrilase family, member 2"
                     /db_xref="GeneID:288174"
                     /db_xref="RGD:1310494"
     exon            1..50
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..874
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /EC_number="3.5.1.3"
                     /note="nitrilase homolog 2; Nit protein 2"
                     /codon_start=1
                     /product="omega-amidase NIT2"
                     /protein_id="NP_001029298.1"
                     /db_xref="GeneID:288174"
                     /db_xref="RGD:1310494"
                     /translation="MSTFRLALIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPEC
                     FNSPYGTNYFPEYAEKIPGESTKKLSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGP
                     DGNLLVKHRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAE
                     LAQIYARRGCQLLVYPGAFNMTTGPAHWELLQRARAVDNQVYVATASPARDEKASYVA
                     WGHSTVVDPWGQVLTKAGTEETILYSDIDLKKLSEIRQQIPILKQKRADLYSVESKKP
                     "
     exon            51..169
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            170..290
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            291..379
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            380..473
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            474..548
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            549..627
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            628..726
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            727..782
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
     exon            783..1031
                     /gene="Nit2"
                     /gene_synonym="RGD1310494"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctggctgcag tccttgcggc gtctggcgct ccggggaaga gccatgtcta ctttccgcct
       61 ggccctcatc cagcttcagg tttcttccat taaatcagat aacattaccc gggcttgtag
      121 cctagtccgg gaggcagcaa agcaaggtgc caacatagtt tctctgccgg agtgcttcaa
      181 ttctccatat ggaactaact actttcctga atacgcagag aagattcctg gagagtccac
      241 aaagaagctt tctgaagtcg caaaggagaa cagcatatac ctcattggag gctccatccc
      301 agaagaggat gatgggaaac tgtataatac ctgcgctgtg tttgggcctg atggaaattt
      361 actggtaaag cacagaaaga tccatctgtt tgacattgat gttcctggga aaattacatt
      421 tcaagaatcc aaaacactga gtcctggtga tagtttctcc acgtttgata ccccttactg
      481 cagagtgggc ctgggcatct gctacgatat gcgcttcgca gagcttgcac aaatctatgc
      541 acgaagaggc tgccagctct tggtgtatcc tggagctttc aatatgacca ctggaccagc
      601 ccactgggag ttgcttcagc gagcccgagc tgttgataat caggtgtatg tggctacagc
      661 ctctcctgcc cgggatgaga aagcctcgta cgtggcctgg ggacacagca ctgtggtgga
      721 tccttggggg caggtcctaa ccaaagctgg caccgaggaa acaatcctgt actcagacat
      781 agacctgaag aagctgtctg aaattcggca gcaaattccc attttaaaac agaaacgagc
      841 agacctctat tcagtggagt caaagaagcc ttaatggctt tctaacctgt cacaagcaga
      901 atgatgttct gtaagacgag caactctacc actctaccaa atcatttttg ggatagggcc
      961 ctttcatgtt aattctacca atgatctgtg acaccaggcc ccctatttta attaaaagat
     1021 ccgtcttcaa aaaaaaaaaa aaaaaaaaaa aaaa
//
