LOCUS       NM_182668               1391 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus nitrilase 1 (Nit1), transcript variant 1, mRNA.
ACCESSION   NM_182668
VERSION     NM_182668.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Peracchi,A., Veiga-da-Cunha,M., Kuhara,T., Ellens,K.W., Paczia,N.,
            Stroobant,V., Seliga,A.K., Marlaire,S., Jaisson,S., Bommer,G.T.,
            Sun,J., Huebner,K., Linster,C.L., Cooper,A.J.L. and Van
            Schaftingen,E.
  TITLE     Nit1 is a metabolite repair enzyme that hydrolyzes deaminated
            glutathione
  JOURNAL   Proc Natl Acad Sci U S A 114 (16), E3233-E3242 (2017)
   PUBMED   28373563
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   3  (bases 1 to 1391)
  AUTHORS   Barglow,K.T., Saikatendu,K.S., Bracey,M.H., Huey,R., Morris,G.M.,
            Olson,A.J., Stevens,R.C. and Cravatt,B.F.
  TITLE     Functional proteomic and structural insights into molecular
            recognition in the nitrilase family enzymes
  JOURNAL   Biochemistry 47 (51), 13514-13523 (2008)
   PUBMED   19053248
REFERENCE   4  (bases 1 to 1391)
  AUTHORS   Semba,S., Han,S.Y., Qin,H.R., McCorkell,K.A., Iliopoulos,D.,
            Pekarsky,Y., Druck,T., Trapasso,F., Croce,C.M. and Huebner,K.
  TITLE     Biological functions of mammalian Nit1, the counterpart of the
            invertebrate NitFhit Rosetta stone protein, a possible tumor
            suppressor
  JOURNAL   J Biol Chem 281 (38), 28244-28253 (2006)
   PUBMED   16864578
REFERENCE   5  (bases 1 to 1391)
  AUTHORS   Pace,H.C. and Brenner,C.
  TITLE     The nitrilase superfamily: classification, structure and function
  JOURNAL   Genome Biol 2 (1), REVIEWS0001 (2001)
   PUBMED   11380987
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000013.1 and
            BC087146.1.
            
            On Mar 10, 2007 this sequence version replaced NM_182668.1.
            
            Summary: The protein encoded by this gene is a member of the
            nitrilase enzyme superfamily. Nitrilases are nonpeptidic C-N
            hydrolases. In mammals, nitrilases form small molecule C-N bonds
            such as acid amides, carbamates and ureas. Nit1-deficient mice
            display phenotypes including increased cell proliferation,
            resistance to DNA damage, and an increased number of
            N-nitrosomethylbenzylamine-induced murine forestomach tumors,
            suggesting a tumor suppressor function. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Mar
            2015].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087146.1, SRR26643291.34328.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                JAXUCZ010000013.1  86305752-86305770   c
            20-1391             BC087146.1         1-1372
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q24"
     gene            1..1391
                     /gene="Nit1"
                     /note="nitrilase 1"
                     /db_xref="GeneID:289222"
                     /db_xref="RGD:727821"
     exon            1..77
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1059
                     /gene="Nit1"
                     /EC_number="3.5.1.128"
                     /note="isoform a is encoded by transcript variant 1;
                     nitrilase homolog 1; dGSH amidase; deaminated glutathione
                     amidase"
                     /codon_start=1
                     /product="deaminated glutathione amidase isoform a"
                     /protein_id="NP_872609.2"
                     /db_xref="GeneID:289222"
                     /db_xref="RGD:727821"
                     /translation="MLGFITRPPHQLLSLLCTGYRLPQISVLCTQPRPRAMAESSSTS
                     WELPLVAVCQVTSTPNKQENFKTCAELVQEATRLGACLAFLPEAFDFIARNPAETLLL
                     SEPLDGDLLGQYSQLARECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASY
                     RKTHLCDVEIPGQGPMRESNYTMPGYALEPPVKTPAGKVGLAICYDMRFPELSLKLAQ
                     AGAEILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVV
                     DPWGTVVASCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLYGSLLPLS"
     exon            78..173
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
     exon            174..431
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
     exon            432..535
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..669
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
     exon            670..795
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
     exon            796..1353
                     /gene="Nit1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccacttgct cctgcgcttc tggcttctgg cagctcttca gatccgaccc tgcgaatggt
       61 tttggctgtg tcttcatgct gggcttcatc accaggcctc ctcatcaact cctgtccctt
      121 ctgtgtaccg gataccgatt acctcaaatc tcagtacttt gtactcagcc caggcccaga
      181 gccatggcag agtcctcatc gacttcctgg gagctgcccc tggtggctgt gtgccaggta
      241 acatcaacac caaacaagca agagaacttt aaaacgtgtg ctgagctggt tcaagaggct
      301 accagactgg gcgcttgcct ggcctttctg cctgaggcat ttgactttat tgcacgaaat
      361 cctgccgaga cattactcct gtccgaacca ctggatgggg accttttggg acaatatagc
      421 cagcttgcca gggaatgtgg aatctggctg tccttgggtg gtttccacga gcgtggccaa
      481 gactgggagc agactcagaa aatctacaat tgtcatgtgc ttctgaacag caagggatca
      541 gtagtggcca gttacaggaa gacacatcta tgtgatgtag agatcccagg tcaggggcct
      601 atgagagaaa gcaactatac catgcctgga tacgctcttg aaccacctgt caagacacca
      661 gcaggcaagg ttggtctagc aatctgttat gacatgcggt tccctgaact ttctctgaaa
      721 ttggctcaag ctggggcaga aatacttact tatccttcag cctttggatc tgttaccggc
      781 ccagcccact gggaggtgct gctgcgggcc cgcgccattg agtctcagtg ctacgtgata
      841 gcagcagcac agtgtggacg ccaccatgag acaagagcaa gttacggcca cagcatggtg
      901 gttgacccct ggggcacagt ggtggccagc tgttccgagg gaccaggcct ttgccttgct
      961 cgaattgatc tccactttct gcaacagatg cgccaacact tgcctgtgtt ccagcatcgc
     1021 agacctgacc tttatggcag tctcctgcca ctctcctaat gtttttaatt tctgttggtt
     1081 gagacagaca aacacacgcc aactgtgagg tgatgctacc gtgacttgta ggctggaccc
     1141 aagcacggtt cccttcactt gcaggactta actctcctaa tggaacacag gctcaatcaa
     1201 gtccatggga aagaagagac tttgacctga tctcagattt cagtttcaga aaaatgaaat
     1261 tttatatagt cactgtttat ttcatgaaaa ctgaagttgt gctgagggct gagcagcact
     1321 ggcattgaaa aaaaatataa taatcaatca taaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1381 aaaaaaaaaa a
//
