LOCUS       NM_001106606            1109 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001106606 XM_001070447 XM_216202
VERSION     NM_001106606.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   James,J., Zemskova,M., Eccles,C.A., Varghese,M.V., Niihori,M.,
            Barker,N.K., Luo,M., Mandarino,L.J., Langlais,P.R., Rafikova,O. and
            Rafikov,R.
  TITLE     Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary
            Artery Smooth Muscle Cells
  JOURNAL   Arterioscler Thromb Vasc Biol 41 (2), 734-754 (2021)
   PUBMED   33297749
  REMARK    GeneRIF: Single Mutation in the NFU1 Gene Metabolically Reprograms
            Pulmonary Artery Smooth Muscle Cells.
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Wesley,N.A., Wachnowsky,C., Fidai,I. and Cowan,J.A.
  TITLE     Understanding the molecular basis for multiple mitochondrial
            dysfunctions syndrome 1 (MMDS1): impact of a disease-causing
            Gly189Arg substitution on NFU1
  JOURNAL   FEBS J 284 (22), 3838-3848 (2017)
   PUBMED   28906594
REFERENCE   3  (bases 1 to 1109)
  AUTHORS   Maio,N. and Rouault,T.A.
  TITLE     Iron-sulfur cluster biogenesis in mammalian cells: New insights
            into the molecular mechanisms of cluster delivery
  JOURNAL   Biochim Biophys Acta 1853 (6), 1493-1512 (2015)
   PUBMED   25245479
  REMARK    Review article
REFERENCE   4  (bases 1 to 1109)
  AUTHORS   Ganesh,S., Tsurutani,N., Suzuki,T., Ueda,K., Agarwala,K.L.,
            Osada,H., Delgado-Escueta,A.V. and Yamakawa,K.
  TITLE     The Lafora disease gene product laforin interacts with HIRIP5, a
            phylogenetically conserved protein containing a NifU-like domain
  JOURNAL   Hum Mol Genet 12 (18), 2359-2368 (2003)
   PUBMED   12915448
REFERENCE   5  (bases 1 to 1109)
  AUTHORS   Tong,W.H., Jameson,G.N., Huynh,B.H. and Rouault,T.A.
  TITLE     Subcellular compartmentalization of human Nfu, an iron-sulfur
            cluster scaffold protein, and its ability to assemble a [4Fe-4S]
            cluster
  JOURNAL   Proc Natl Acad Sci U S A 100 (17), 9762-9767 (2003)
   PUBMED   12886008
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FM058785.1, FM064033.1 and FM092780.1.
            
            On Dec 25, 2009 this sequence version replaced NM_001106606.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132262,
                              SAMD00132263 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-553               FM058785.1         1-553
            554-827             FM064033.1         380-653
            828-1109            FM092780.1         211-492
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..1109
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /note="NFU1 iron-sulfur cluster scaffold"
                     /db_xref="GeneID:297416"
                     /db_xref="RGD:1307823"
     exon            1..61
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     CDS             3..764
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /note="histone cell cycle regulation defective interacting
                     protein 5; NFU1 iron-sulfur cluster scaffold homolog,
                     mitochondrial"
                     /codon_start=1
                     /product="NFU1 iron-sulfur cluster scaffold homolog,
                     mitochondrial precursor"
                     /protein_id="NP_001100076.2"
                     /db_xref="GeneID:297416"
                     /db_xref="RGD:1307823"
                     /translation="MAAAERAWGAAVGVARLCRRLCHVATPHAFKKQPLHQYVQRPLF
                     PLRTPLYNTVRFMFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLF
                     RIEGVKSVFFGPDFITVTKENEELDWNLLKPDIYATIMDFFASGLPLVTEETPPGEAG
                     SEEDDEVVAMIKELLDTRIRPTVQEDGGDVIYRGFEDGIVRLKLQGSCTSCPSSIITL
                     KSGIQNMLQFYIPEVEGVEQVMDDEESDEKEANSP"
     exon            62..162
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     exon            163..298
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     exon            299..365
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     exon            366..480
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     exon            481..541
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     exon            542..716
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
     exon            717..1109
                     /gene="Nfu1"
                     /gene_synonym="Hirip5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgatggcggc ggccgagcgg gcgtggggag cagctgtcgg tgtagcccgg ctgtgcaggc
       61 ggctctgtca tgtggcgact ccacacgcct ttaagaaaca gcctctgcac caatatgtac
      121 agagaccact cttccctctg cgcacgccct tgtataacac agtacgattc atgtttattc
      181 aaacacaaga caccccaaat cccaacagct taaagtttat accaggaaag ccggttcttg
      241 agacgaggac catggatttc cccacaccag ctgcggcttt ccgttcccct ctggccaggc
      301 agttattcag gattgaaggt gtgaaaagtg tcttctttgg gccagatttc attacagtca
      361 caaaggagaa tgaagaatta gactggaatt tactgaaacc agacatttat gcaacaatta
      421 tggacttctt tgcatctggc ttacctctag tgactgagga gacgcctcca ggagaagcag
      481 gttctgagga agatgacgaa gttgtggcaa tgattaaaga actgttagat actagaatac
      541 ggccgactgt gcaggaggat gggggtgacg tgatctacag aggctttgaa gatggcatcg
      601 tgcggctgaa gctccagggc tcctgcacca gctgccccag ctctatcatc accctgaaga
      661 gcgggattca gaacatgctg cagttttata ttccagaagt ggaaggcgtg gagcaggtga
      721 tggatgatga ggaatcagat gaaaaagaag caaattcacc ttaaataatc tgcattttct
      781 ttggccacaa ccgttggact tatctttact gagcctgtat tactgctgtg taagtttatt
      841 aataaaaggt ttcctttctc cagaatgaga tgatatataa gtataaatat atatatttat
      901 atttgcttcc cctcgcaaat tatttacacc atcctgaggt gccaccctga gtcacccacc
      961 ctcttgggtc ttaatcattt atgctgccct gagccttatc cccacacttt ctttcatgct
     1021 gggagaagca ttttaaaata catttcccat ttaacccatt tgcttttttt atacagaaat
     1081 aaacagaata aatgcattta aaatatcct
//
