LOCUS       NM_053462               2038 bp    mRNA    linear   ROD 03-FEB-2024
DEFINITION  Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), mRNA.
ACCESSION   NM_053462
VERSION     NM_053462.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Shan,Y. and Cortopassi,G.
  TITLE     Mitochondrial Hspa9/Mortalin regulates erythroid differentiation
            via iron-sulfur cluster assembly
  JOURNAL   Mitochondrion 26, 94-103 (2016)
   PUBMED   26702583
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Maio,N. and Rouault,T.A.
  TITLE     Iron-sulfur cluster biogenesis in mammalian cells: New insights
            into the molecular mechanisms of cluster delivery
  JOURNAL   Biochim Biophys Acta 1853 (6), 1493-1512 (2015)
   PUBMED   25245479
  REMARK    Review article
REFERENCE   3  (bases 1 to 2038)
  AUTHORS   Tsai,C.L. and Barondeau,D.P.
  TITLE     Human frataxin is an allosteric switch that activates the Fe-S
            cluster biosynthetic complex
  JOURNAL   Biochemistry 49 (43), 9132-9139 (2010)
   PUBMED   20873749
REFERENCE   4  (bases 1 to 2038)
  AUTHORS   Fosset,C., Chauveau,M.J., Guillon,B., Canal,F., Drapier,J.C. and
            Bouton,C.
  TITLE     RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity
            both in mitochondria and cytosol of mammalian cells
  JOURNAL   J Biol Chem 281 (35), 25398-25406 (2006)
   PUBMED   16787928
REFERENCE   5  (bases 1 to 2038)
  AUTHORS   Biederbick,A., Stehling,O., Rosser,R., Niggemeyer,B., Nakai,Y.,
            Elsasser,H.P. and Lill,R.
  TITLE     Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein
            biogenesis and iron regulation
  JOURNAL   Mol Cell Biol 26 (15), 5675-5687 (2006)
   PUBMED   16847322
REFERENCE   6  (bases 1 to 2038)
  AUTHORS   Li,K., Tong,W.H., Hughes,R.M. and Rouault,T.A.
  TITLE     Roles of the mammalian cytosolic cysteine desulfurase, ISCS, and
            scaffold protein, ISCU, in iron-sulfur cluster assembly
  JOURNAL   J Biol Chem 281 (18), 12344-12351 (2006)
   PUBMED   16527810
REFERENCE   7  (bases 1 to 2038)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 2038)
  AUTHORS   Nakai,Y., Yoshihara,Y., Hayashi,H. and Kagamiyama,H.
  TITLE     cDNA cloning and characterization of mouse nifS-like protein,
            m-Nfs1: mitochondrial localization of eukaryotic NifS-like proteins
  JOURNAL   FEBS Lett 433 (1-2), 143-148 (1998)
   PUBMED   9738949
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC104699.1.
            
            [WARNING] On Apr 23, 2024 this sequence was replaced by
            NM_053462.3.
            
            On Oct 4, 2005 this sequence version replaced NM_053462.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC104699.1, BC089205.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760494 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..2038
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /note="NFS1 cysteine desulfurase"
                     /db_xref="GeneID:84594"
                     /db_xref="RGD:620912"
     exon            1..136
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..1395
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /EC_number="2.8.1.7"
                     /note="cysteine desulfurase, mitochondrial; nitrogen
                     fixation gene 1; NFS1 nitrogen fixation 1 homolog;
                     cysteine desulfurase, mitochondrial-like"
                     /codon_start=1
                     /product="cysteine desulfurase"
                     /protein_id="NP_445914.2"
                     /db_xref="GeneID:84594"
                     /db_xref="RGD:620912"
                     /translation="MVGSVAGNMLLRAAWRRASLAATSVALRRSSVPTRGLRLRVVDH
                     APHSAVPSEAEAVLRPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESE
                     AAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKC
                     VLDSCRSLEAEGFRVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIA
                     EIGQICSSRKLYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRP
                     RVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQK
                     IMKNLPDVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSY
                     VLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVQKCIHHVKRLREMSPLWEMVQDGID
                     LKSIKWTQH"
     exon            137..228
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            229..345
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            346..429
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            430..582
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            583..676
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            677..811
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            812..969
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1075
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1157
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1241
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1331
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..2009
                     /gene="Nfs1"
                     /gene_synonym="Nifs"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttgcaagcga cgcagatggt gggctctgtt gctgggaaca tgctgctgcg agccgcttgg
       61 aggcgagcgt cgttggcggc tacctctgtg gccctgcgaa ggtcctcggt gcccacccgg
      121 ggactgcgcc tgcgcgttgt agatcatgcc ccccattcgg ctgttccctc cgaggcagag
      181 gcagtgctgc gacctctcta tatggacgtg caggctacaa ctcctctgga ccccagggtg
      241 cttgatgcca tgctcccata ccttgtcaac tactatggga acccacattc tcggactcat
      301 gcgtatggct gggagagcga ggcagccatg gaacgtgctc gccagcaggt agcgtctctg
      361 attggagcgg atcctcggga gatcattttc actagtggag ctacggaatc caacaacata
      421 gcaattaagg gagtggccag gttctacagg tcacggaaaa agcacttggt caccacgcag
      481 acagaacaca agtgcgtgct ggattcgtgc cgctccctgg aagctgaggg ctttcgggtc
      541 acttacctcc ccgttcagaa gagtgggatc attgacttaa aggaactaga ggctgccatc
      601 cagccagata ccagcctggt ctccgttatg actgtaaaca atgagattgg agtaaagcaa
      661 ccgattgcag aaataggcca gatttgcagt tccagaaagc tgtacttcca tactgatgca
      721 gcccaagctg ttgggaaaat cccacttgat gtcaatgaca tgaaaattga tctcatgagt
      781 atcagtggtc acaaactcta cggccctaaa ggggttggtg ctatctatat ccgccgtagg
      841 ccccgggtgc gtgtggaggc cctacaaagt ggaggcgggc aggagcgggg tatgcggtct
      901 gggacagtgc ccacaccctt ggtggtgggc ctgggagctg catgtgagct ggcacaacaa
      961 gagatggagt acgaccataa acggatctca aagttagcag agcgactcat acagaagata
     1021 atgaagaacc ttccagatgt ggtgatgaac ggggacccga agcagcacta ccctggctgt
     1081 atcaacctct cctttgcgta tgtggaaggg gaaagcctgc tgatggcact caaggacgtt
     1141 gccttgtcct cggggagtgc ctgcacttcc gcatcattgg agccctcgta tgtcctcaga
     1201 gccatcggta cggatgagga tttagcacac tcctctatca ggttcggcat cggccgcttc
     1261 actacagagg aggaggtaga ctacaccgtg cagaagtgca tccaccatgt gaaacgtctt
     1321 cgagaaatga gtcctctctg ggagatggtg caggatggca ttgacctcaa gagcatcaag
     1381 tggacccagc attagaagag tggactgtga cttcgtgctg gcctggctgc tctggcctct
     1441 ccgacccatg cacagccagg aacctcatta catgctcaag tcagattgga agttcttcag
     1501 tggcttcacc acagaatgtt ttcaagtgag aacttgaagg gaggatcttt gctagcctca
     1561 gctcctctgt agacacgttc cctattctcc tcaggagctc ttctccgctg tctagtgtgg
     1621 ccattcttac ctgaggacct gtaggcactg ctgtgttact gccacgggga ctacctaccc
     1681 gtgggcagat gaaggaacta gaagaaggct ctaggttcaa agactgcttc tctgtgaatc
     1741 ttcactcttg ctgctcagct ggactggcgc cattagggac aggcataaac aatctttctt
     1801 ttaacctttt ttgtggcttc aaaagacatc ttttatatca atagcaagtt gtattaaagt
     1861 ctcgtttcct ttggggtctc agcttctttt ctgtattttg atttaatgta gaaatatcca
     1921 tgactccacc cattattcta ataatttgct tagttcatta aactttgttg atttgaagat
     1981 tggagaaata aattccattt cccctcttaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//
