LOCUS       NM_001160314            9810 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus neurofascin (Nfasc), transcript variant 1, mRNA.
ACCESSION   NM_001160314
VERSION     NM_001160314.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Monfrini,E., Straniero,L., Bonato,S., Monzio Compagnoni,G.,
            Bordoni,A., Dilena,R., Rinchetti,P., Silipigni,R., Ronchi,D.,
            Corti,S., Comi,G.P., Bresolin,N., Duga,S. and Di Fonzo,A.
  TITLE     Neurofascin (NFASC) gene mutation causes autosomal recessive ataxia
            with demyelinating neuropathy
  JOURNAL   Parkinsonism Relat Disord 63, 66-72 (2019)
   PUBMED   30850329
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Saha,R., Kriebel,M., Volkmer,H., Richter-Levin,G. and Albrecht,A.
  TITLE     Neurofascin Knock Down in the Basolateral Amygdala Mediates
            Resilience of Memory and Plasticity in the Dorsal Dentate Gyrus
            Under Stress
  JOURNAL   Mol Neurobiol 55 (9), 7317-7326 (2018)
   PUBMED   29404957
  REMARK    GeneRIF: The knock down of neurofascin in the basolateral amygdala
            prevented a priming-induced impairment on long-term potentiation
            maintenance in the dentate gyrus.
REFERENCE   3  (bases 1 to 9810)
  AUTHORS   Gumy,L.F., Katrukha,E.A., Grigoriev,I., Jaarsma,D., Kapitein,L.C.,
            Akhmanova,A. and Hoogenraad,C.C.
  TITLE     MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus
            KIF5-Dependent Cargo Transport in Sensory Neurons
  JOURNAL   Neuron 94 (2), 347-362 (2017)
   PUBMED   28426968
REFERENCE   4  (bases 1 to 9810)
  AUTHORS   Hu,B., Arpag,S., Zhang,X., Mobius,W., Werner,H., Sosinsky,G.,
            Ellisman,M., Zhang,Y., Hamilton,A., Chernoff,J. and Li,J.
  TITLE     Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP
  JOURNAL   PLoS Genet 12 (9), e1006290 (2016)
   PUBMED   27583434
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9810)
  AUTHORS   Sohn,P.D., Tracy,T.E., Son,H.I., Zhou,Y., Leite,R.E., Miller,B.L.,
            Seeley,W.W., Grinberg,L.T. and Gan,L.
  TITLE     Acetylated tau destabilizes the cytoskeleton in the axon initial
            segment and is mislocalized to the somatodendritic compartment
  JOURNAL   Mol Neurodegener 11 (1), 47 (2016)
   PUBMED   27356871
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9810)
  AUTHORS   Tait,S., Gunn-Moore,F., Collinson,J.M., Huang,J., Lubetzki,C.,
            Pedraza,L., Sherman,D.L., Colman,D.R. and Brophy,P.J.
  TITLE     An oligodendrocyte cell adhesion molecule at the site of assembly
            of the paranodal axo-glial junction
  JOURNAL   J Cell Biol 150 (3), 657-666 (2000)
   PUBMED   10931875
REFERENCE   7  (bases 1 to 9810)
  AUTHORS   Collinson,J.M., Marshall,D., Gillespie,C.S. and Brophy,P.J.
  TITLE     Transient expression of neurofascin by oligodendrocytes at the
            onset of myelinogenesis: implications for mechanisms of axon-glial
            interaction
  JOURNAL   Glia 23 (1), 11-23 (1998)
   PUBMED   9562181
REFERENCE   8  (bases 1 to 9810)
  AUTHORS   Davis,J.Q., Lambert,S. and Bennett,V.
  TITLE     Molecular composition of the node of Ranvier: identification of
            ankyrin-binding cell adhesion molecules neurofascin (mucin+/third
            FNIII domain-) and NrCAM at nodal axon segments
  JOURNAL   J Cell Biol 135 (5), 1355-1367 (1996)
   PUBMED   8947556
REFERENCE   9  (bases 1 to 9810)
  AUTHORS   Davis,J.Q. and Bennett,V.
  TITLE     Ankyrin binding activity shared by the neurofascin/L1/NrCAM family
            of nervous system cell adhesion molecules
  JOURNAL   J Biol Chem 269 (44), 27163-27166 (1994)
   PUBMED   7961622
REFERENCE   10 (bases 1 to 9810)
  AUTHORS   Davis,J.Q., McLaughlin,T. and Bennett,V.
  TITLE     Ankyrin-binding proteins related to nervous system cell adhesion
            molecules: candidates to provide transmembrane and intercellular
            connections in adult brain
  JOURNAL   J Cell Biol 121 (1), 121-133 (1993)
   PUBMED   8458865
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JACYVU010000242.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001160314.3.
            
            On Feb 11, 2021 this sequence version replaced NM_001160314.1.
            
            Summary: This gene encodes an L1 family immunoglobulin cell
            adhesion molecule with multiple IGcam and fibronectin domains. The
            protein functions in neurite outgrowth, neurite fasciculation, and
            organization of the axon initial segment (AIS) and nodes of Ranvier
            on axons during early development. Both the AIS and nodes of
            Ranvier contain high densities of voltage-gated Na+ (Nav) channels
            which are clustered by interactions with cytoskeletal and
            scaffolding proteins including this protein, gliomedin, ankyrin 3
            (ankyrin-G), and betaIV spectrin. This protein links the AIS
            extracellular matrix to the intracellular cytoskeleton. This gene
            undergoes extensive alternative splicing, and the full-length
            nature of some variants has not been determined.[provided by
            RefSeq, May 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). This isoform lacks
            the third fibronectin type 3 (FNIII) repeat and includes the
            mucin-like domain. Isoform 1 is also known as NF186, as described
            by Davis et al. (PMID: 8947556).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: U81036.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMD00132261,
                                    SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               JACYVU010000242.1  30544982-30545081   c
            101-218             JACYVU010000242.1  30458992-30459109   c
            219-399             JACYVU010000242.1  30436800-30436980   c
            400-417             JACYVU010000242.1  30431297-30431314   c
            418-523             JACYVU010000242.1  30430440-30430545   c
            524-720             JACYVU010000242.1  30428176-30428372   c
            721-843             JACYVU010000242.1  30427767-30427889   c
            844-1014            JACYVU010000242.1  30425652-30425822   c
            1015-1126           JACYVU010000242.1  30414817-30414928   c
            1127-1311           JACYVU010000242.1  30414227-30414411   c
            1312-1443           JACYVU010000242.1  30412984-30413115   c
            1444-1587           JACYVU010000242.1  30405188-30405331   c
            1588-1699           JACYVU010000242.1  30404525-30404636   c
            1700-1866           JACYVU010000242.1  30404061-30404227   c
            1867-2014           JACYVU010000242.1  30403579-30403726   c
            2015-2139           JACYVU010000242.1  30402238-30402362   c
            2140-2184           JACYVU010000242.1  30400756-30400800   c
            2185-2286           JACYVU010000242.1  30399034-30399135   c
            2287-2484           JACYVU010000242.1  30398614-30398811   c
            2485-2555           JACYVU010000242.1  30397287-30397357   c
            2556-2778           JACYVU010000242.1  30395594-30395816   c
            2779-2901           JACYVU010000242.1  30390310-30390432   c
            2902-3075           JACYVU010000242.1  30389272-30389445   c
            3076-3090           JACYVU010000242.1  30387205-30387219   c
            3091-3327           JACYVU010000242.1  30381788-30382024   c
            3328-3444           JACYVU010000242.1  30377897-30378013   c
            3445-3597           JACYVU010000242.1  30376497-30376649   c
            3598-3729           JACYVU010000242.1  30370431-30370562   c
            3730-3799           JACYVU010000242.1  30368012-30368081   c
            3800-9810           JACYVU010000242.1  30358493-30364503   c
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     gene            1..9810
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /note="neurofascin"
                     /db_xref="GeneID:116690"
                     /db_xref="RGD:620911"
     exon            1..100
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            101..218
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            219..399
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..4031
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="neurofascin isoform 1 precursor"
                     /protein_id="NP_001153786.1"
                     /db_xref="GeneID:116690"
                     /db_xref="RGD:620911"
                     /translation="MARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTIT
                     KQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVID
                     FRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTL
                     QCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFH
                     FTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTP
                     DIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVK
                     AAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDT
                     IIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRL
                     DCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILG
                     KAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYI
                     GNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALP
                     KGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFP
                     GSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTR
                     KNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYE
                     IRVQAENDFGKGPEPETVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILI
                     GYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATE
                     ESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVA
                     TTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWK
                     HNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISST
                     VITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKD
                     VPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNE
                     DGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA"
     exon            400..417
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            418..523
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            524..720
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            721..843
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            844..1014
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1126
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1311
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1443
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1587
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1699
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1866
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..2014
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2139
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2184
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2286
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2484
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2555
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2778
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2779..2901
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..3075
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3090
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3327
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3444
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3597
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3598..3729
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3730..3799
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
     exon            3800..9810
                     /gene="Nfasc"
                     /gene_synonym="NF"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctggcggc gagagctgct gcaggggacg cgggcgaagt ggcggcgccg ggagcagaca
       61 tctgggactg cgcccagggc tggagctgga gctcctggag gttgactgac ttatgtgcga
      121 tttgggagcc tggagttttc ttgccctctg ccgctgccgt caaccggagc agagcctccg
      181 ctggtgttgc gaaaagcagg ccgaatgagg cagagaagct gagcactgtc tggaaagccc
      241 acgaggaacg gcgcaaggca tcgagagccc gtgggctggg cagtgcggag cagggcctgg
      301 cgctgaggat ggccaggcag caggcgccac cctgggtcca cgtagccctc atcctcttcc
      361 tcctcagcct cggaggggcc attgagattc cgatggatcc aagcattcag aatgagctga
      421 cccaaccccc aacgatcacc aagcagtcgg tgaaggacca catcgtggac ccccgagata
      481 acatcctgat tgaatgtgaa gctaaaggga accccgcccc cagtttccac tggactcgca
      541 acagcaggtt cttcaacatt gccaaggacc cacgggtgtc catgaggagg aggtctggga
      601 ccttggtgat cgacttccgc agtggtgggc ggcctgagga gtacgaaggg gagtaccagt
      661 gctttgcccg gaacaaattc ggcacagctc ttagcaaccg catccgcctg caggtgtcca
      721 aatctcccct gtggcccaag gaaaacctag accccgtcgt ggttcaagag ggtgccccct
      781 taaccctgca gtgcaacccc ccacctggcc tcccatcccc cgtcatcttc tggatgagca
      841 gctccatgga gcccatcacc caggacaagc gtgtctccca gggtcacaac ggggacctgt
      901 acttctccaa cgtcatgctg caggacatgc agaccgacta cagctgcaat gcacgcttcc
      961 acttcaccca caccattcag cagaagaatc ccttcaccct caaagtcctc accacccgag
     1021 gagttgcgga aagaacacct agcttcatgt atccccaggg cacgtcaagc agtcagatgg
     1081 tactgcgcgg catggacctg ctgctggagt gcattgcctc tggggtccca acaccagata
     1141 ttgcatggta caagaaaggt ggggacctcc catctgacaa ggccaagttc gagaatttta
     1201 acaaggctct gcgcatcacc aatgtctctg aagaggactc tggggagtat ttctgcctgg
     1261 cctccaacaa gatgggcagc atccggcaca cgatctcggt gagagtaaag gctgccccat
     1321 actggctgga tgagcccaag aacttgatcc tggctcctgg tgaagatggg aggctggtgt
     1381 gtcgagccaa tgggaacccg aagccgaccg tccagtggct ggtgaatgga gaccctttgc
     1441 aatcggcacc acccaacccc aaccgtgagg tggccggaga cactatcatc ttccgggaca
     1501 ctcagatcag cagcagggca gtgtaccagt gtaacacatc caacgaacat ggctacctgc
     1561 tggccaatgc cttcgtcagc gtattagatg taccccctcg gatgctgtct ccccggaacc
     1621 agctcatcag ggtgatcctt tacaaccgga cgcgactgga ctgtccgttc tttgggtctc
     1681 ccattccaac actccgatgg tttaagaatg ggcaaggaag caacctggat ggtggtaact
     1741 accacgtcta cgaaaatggc agcctggaaa tcaagatgat tcgcaaagag gaccaaggca
     1801 tctacacctg tgtggccacc aacatcctgg gaaaagctga aaatcaagtc cgcctggagg
     1861 tcaaagaccc caccaggatc tacaggatgc ctgaagacca ggtggccaag aggggcacca
     1921 cagtgcagct ggagtgccgt gtgaagcatg acccctcctt gaaactcaca gtctcctggc
     1981 tgaaggatga cgagccactc tacattggaa acaggatgaa aaaggaagat gactccctga
     2041 ccatcttcgg agtggcagag cgggaccaag gcagttacac gtgcatggcc agcaccgagc
     2101 tggaccagga cctggcaaag gcctacctca ctgttctagc tgatcaggcc actccgacta
     2161 accgtttggc tgccctgccc aaagggcggc cagaccgacc cagggacctg gagctcactg
     2221 acctggcgga aaggagtgtg aggctgacct ggatcccggg ggatgataac aacagtccca
     2281 tcacagacta cgttgtccag tttgaagagg accagttcca gccaggagtc tggcatgacc
     2341 actccaagtt cccaggcagt gtcaactcag ccgtcctcca tctgtccccg tatgtcaact
     2401 atcagttccg agtcatcgct gtcaacgagg ttgggagcag ccaccccagc cttccatccg
     2461 agcggtaccg aacaagcggg gcaccccctg aatccaaccc cagtgatgtg aagggcgaag
     2521 ggacacgaaa aaacaatatg gagatcacat ggacgcctat gaatgctacc tccgcctttg
     2581 gccccaacct gcgctacatt gtcaagtggc gacggagaga aacccgagag acttggaaca
     2641 atgtcaccgt gtggggctct cgctatgtgg tggggcagac ccctgtctac gtaccctatg
     2701 agatccgagt ccaggctgaa aatgactttg ggaaaggccc ggagcctgaa accgtcattg
     2761 ggtactcggg ggaagattta cccagtgccc ccaggcgttt cagagtccga cagcccaacc
     2821 tggagaccat caacctggaa tgggatcacc cagagcaccc caacgggatc ctgattggat
     2881 acacgctcag atacgtgccc tttaatggaa ccaaactggg aaagcagatg gtggaaaact
     2941 tctctcccaa tcagaccaag ttctcggtgc agagagcaga ccccgtgtcc cgttaccgct
     3001 tctccctcag tgccaggacg caggtgggct ctggagaagc agccacggag gagtccccaa
     3061 cacctccaaa tgaagctact ccaactgcag ctcctcccac attgcccccg actaccgtgg
     3121 gtaccacagg ccttgtgagc agtactgatg ctactgccct tgctgccacc agtgaagcca
     3181 caacagttcc catcatccca accgtcgtac ctaccaccgt cgccaccacc attgccacaa
     3241 ctactacaac cactgccgcc gccaccacca ctaccaccac ggagagtcct cccactacca
     3301 ccactgggac taagattcac gaaaccgccc ccgacgagca gtccatttgg aacgtcacgg
     3361 tgctccccaa cagtaaatgg gccaacatca cctggaagca caatttcagg cctggaactg
     3421 actttgtggt tgagtacatc gacagcaacc atacgaaaaa aactgtccct gttaaggccc
     3481 aagcccagcc catacagctg acagacctct ttcccgggat gacgtacacg ttgcgggtgt
     3541 attcccggga caacgagggc atcagcagta ccgtcatcac ctttatgacc agtacagctt
     3601 acaccaacaa ccagactgac atcgccaccc agggctggtt catcgggctc atgtgtgcca
     3661 ttgcccttct ggtgctgatc ctgcttatcg tctgcttcat caagaggagt cgtggcggca
     3721 agtacccagt gcgagaaaag aaggatgtcc ccttgggccc tgaagacccc aaagaagaag
     3781 atggttcatt tgactacagt gacgaggaca acaagcccct gcagggcagc cagacatctc
     3841 tggacggcac catcaagcag caggagagtg acgacagcct agtggactac ggcgagggtg
     3901 gcgagggcca gttcaacgaa gatggctcct ttattggcca gtacactgtc agaaaggaca
     3961 aggaggagac cgagggcaat gagagctcag aggccacatc tccagtcaat gccatctatt
     4021 ccctggcctg atggagcatc ccaggcacag cactactttg caagtaggag gggagagggg
     4081 agaggaagcc actgcagacc tgccaccaag ccacaaccac cttcagggac aagggtacaa
     4141 catgggggtc tgccaagctg tgaggaccag tgaccaccca gctacccaca ccccctccta
     4201 gtgatgcccc attacccttg ggtgccatta gatgggaaag ctgaagttaa cgctcattgg
     4261 agggagccct agtcccttgc cctgtctcac ggccaccctg agcgtcccac cacaatggcc
     4321 accctggatc tcggctcaca ctcacttctt tctattgctt acaatattct cattcatgtt
     4381 ctctctctct ctctctctct ctctctctct ctctctctct ctctctcttt gctttgtgca
     4441 tcttctcctt ccaagtcatt gtctccattt tcttttactt ttggggagaa aaaaagtccc
     4501 cggaaaaagc agaagtgggc tctccccaag gaaacagaat gatgagcata aaaggattga
     4561 tccgtaacgc aacgtgcaga gaggctgtag tattcaagct gccacgaggc agcgtgtttc
     4621 cgaaggatgc ctttcgccat atgcctcccc cacccccact ccccacccat ctgtctcggc
     4681 cgcatccatg ctgagctggg cttggctctt ttctggaaaa atgacagtgt ttttggcagg
     4741 aagaggttga acgggactcc tttccacgtc ctgctccggt tgggaggggg gcttacctct
     4801 cgcctcctgg ttcttactct gcccctgcca ccagagagtc tgtgcaggag ctgctccgaa
     4861 gatgagaaag gactagagac cgatgaggac tgtggcgacc ggaccccaag aaagaagcaa
     4921 gtcagggtgt ggtccctggg tagtggagca aacctccgga gaagaggaag ggctgggttt
     4981 gggcctcttt tgtctctggc agtgaaaccc agggtgaagg agagaggacc acacctgctc
     5041 cacctggtca gggtctatgc tgctactacc ctccaagagc tggagccctg tgattcagga
     5101 gcgtgactgg tgtgtctggg ggcaacagtg ctgctccccc tcccgtcctc tcttgctcaa
     5161 ttgtgtctga cattgacttg tgtttgacat ggtatatgaa tggggatctg ccgcccccag
     5221 actgagccta tctgtccttt cattagggta cacttacata tcgggggacc atgaggtgca
     5281 ccctactaac agtgctgtga tgcccctgcc cagaaggaca aaagcccttt gtatgcctta
     5341 cacagctcgg atctaaccct gcagctccca gatccctagc cctattctgc aagtcttgat
     5401 gcctccagtg atgtgtgtct cgggaccagg gcagcctatg caagtgtccc ccccaccccc
     5461 caggaccagg agcagccact ccacacacac ccttacccct ctctgcccca acggagacac
     5521 tcgcagacct gcagttctca gcctgaaggt gctgccttca tttccccacc taacccactt
     5581 ttgaaaccaa aggtaccttg cctcagcgac acaaagagat gggaggtctt gagccagaat
     5641 gccgctctgt agctgtgtct gcagcctgag gtggttgtgg gaggggcaag gttgaccgca
     5701 gcgaatggat cacctgcctt tggcgtggcc tccaactcca ggtactagct aggggcctgg
     5761 gcaggaccag ccctgcgcgt tctagagact tttcaagagc gtcatttgcc atatgtctga
     5821 gccgcagagg tattaacaat gctcgcatca cttgataagc atttgggtga acatcaagcc
     5881 cataggcacg cactctttgc aggtaagaaa tcatatctca gagatgagcc gataatgttt
     5941 gaacctgaga gtgtgtcatg gtcctttgga ccaacccatg gccagcactg ctgcccaggg
     6001 acaactctca tggtgaaagg ggacagggta aacaggaagg ctgggggtag gggcgggtgt
     6061 gtgctcaggg tccccttccc tggctctaat ctagcacttc tggggcacag ctgggcatta
     6121 ctgcagtgtg ggtcaccatt cctgaaggga gggttcgggg aaaatagctg aagtaaaggc
     6181 tcatcctttc cagaaaacac ttcggacctg gacgaagtgg tcggcttctc aggcgacatt
     6241 tccacagcac cctccagctc gtaaacccat tgcaccaaca gcaggctgtt agaggaaggg
     6301 actctgctgt cattgcagct ggggagggct gctttggtct taagggtctg ggagagagtt
     6361 aaatccttag gctgtctttg tgcctcattc cagagttctg tcgtgctcag cactgtcccc
     6421 tgcctaggaa gaaaagtccg tcagtcagtc cctccctcag gctgttgtgt tgtgggtgct
     6481 gatcgctgtc ccaaccacag cagatcacag ctctcctggc agggacagac cgagggccgg
     6541 agaaccagag aaatggcagg tgcccagaca gagcaggctc agcggtgtga ggtttccagg
     6601 aggcaactct ttccagggag gggagaggtc actttcacag agcctgccag agtctatccc
     6661 ccaactcagc gagaggaccg gtattgccac ccctgcccct ggagcttaag cagccgccca
     6721 acgtgatacc tgatcctgct agtgcttcat ctgtcgggtg ggagcagggg gacggtgtgg
     6781 gtcatagtgc agggattctg agtgcccttt gagcgtctag tctgggccaa agcttaaacc
     6841 atgaacacca tgtgaacgag tgaggtcaga tgcaatccct gcccacacag ggccgatgtt
     6901 actggaatgg agtaggtgag ttgccggcac agcgcgacca ttcagttgga agcattgagt
     6961 tcttgtttgc ctagactgct gattgcttcc aagggagaac ttgctgcatt gggccagcaa
     7021 atctgatggt caagatgaat ggattcagca gccagcgctg tgcttcttta gggtgtctgt
     7081 ctcccacatt taaccccttc cttgaatact cctgtttgga ggagaatagg agtacccgtc
     7141 aggatcagcc gtcccccatc ccacccggac tacgctaaac aggacacatc agtctctctg
     7201 ctgagttaac actagaggga gggaaagcca ggctggctcc tgcccttgcc tagtttctcc
     7261 ttcccagagg tcagtgtagg agggaccacc cctcccgaca aggtctggaa tgattcctgg
     7321 gccaagagtc agccagtgag ggactcagct taccgtaatc tcaaggggaa taagagaacg
     7381 tgttcctggt ttccctaaac acagtcagca ttgagaaggg aggggaaacg tccgtgggtg
     7441 gttgtgggca caacctcccc atccaccata tgcctgtgca tacaccctca cacaagcaga
     7501 ccctgttgca gagcggtgct cccagtaggg agcattctgg atcatgatgt catctcacag
     7561 catgagtatc catacatacc tcggtgcaca tgagatgagc tgctaacagc tgggggaggg
     7621 gggcctaggg gagggagaag ctatccagat tagtccttat ggttcctagg aacagaaggt
     7681 agaaaagaga tctgaaggag ggataaacag gtctttgagt ctcctggcct ctcctgggag
     7741 ccctcagcta agctcggctg tcctagagcc tcttgatggg gaagcaaggt tttcttgcta
     7801 tttatgggag ctgtttcatt gttgctctga ggacatgacg cttctcggtc cctccatggt
     7861 ctaggactga gacccacact cacttctcag tagaaggaga gcaggttgct agaatgccag
     7921 ccgcccagag tccgtacctc atctgtgtca cttcagagtg gggtgtcctc caacgaaggc
     7981 cagcaggctc tgcccaacgc aagcccacgg ccaggattag gtccaaccgt tagccaggcc
     8041 acagtgctgt ctttctagtg aagcaaaagt ccttacatga atttcacacg atagtctagg
     8101 aatcccatgt aggcagttcc cactccaaaa caagaagtct caagccagtt acagagaatg
     8161 tggactgtca ggggcttcga tgccctgggg aacactggca gcttgggcag agtgtctccc
     8221 caactcaatg cctgaaacca gacccatggc tgtccccgcc ctgcacaacc tcagcccaga
     8281 aaagtgccct tgcactgctt cctcttctca gaaggggact ctcagagacg cattcaggag
     8341 aggacagaga aggacatgtg cccggggaag ctgcaagacc cccccccccc cagctatctc
     8401 cagccatggc ggaaaccatt gtttctctta tcagcgagtt agtattaaaa ggctgctgtc
     8461 tgagaaggga gatggagatg aaacagacac aaggcgtcta gctcaggacg gaatctgagt
     8521 cctcgtgtgc aggaacctca gagctgatgt gtttaaaatg actcattcta cagacggggg
     8581 aatgacagtg cagggaggaa gtctggccag ggagggtgca tttaggactt gaacttgggc
     8641 ccttctgggc cttctgactc cgtgtccagt gttctttctt ctcaccacag ctgcctcagc
     8701 aggtgttgcc tgagatccat cctgcttgtc accctgtcac ctctctctac catttcactt
     8761 atgtttccag caccccccag caaacaacaa agaggcattt cctgagtctg agatcagcca
     8821 gtctgtgata gcctgtggtt aacttagcca gggagtccca ggtagcccaa tgctggaagg
     8881 tagaatgatg accccatagg tgtttgttac tgtgaaacct ggactgggcc cggggatggt
     8941 gtctcttgat tcatcatgcg cctggccctc agcacactgt attcctcaga agggagggtg
     9001 aggaggggct tctttgtcca ttctgccctt gaccgccaag tctgcaggtg ttgaggggta
     9061 gcagggcccg ggcaagcctc ccaccctggg atctgccgag ctggagttta gcagacataa
     9121 agacttctat gaagcaataa acacaaaagt ctgggagaag atatccagag ttttgtgcga
     9181 tcctgtttct ttttcaaagc tgtagcagca gatcaggtga ccctgacctg cttgctcctc
     9241 tatccaatgt gatgtcctca gtcagtgttt gccctctacc cggcttccca gctctttgcc
     9301 aacttcttct gagctgagtt atcagtcacc tgtgacacgc ccgctctctg tcctggtccc
     9361 gccctcaact atggtggatc ctggaggacc tcttacccct cccttccccg acatatgcat
     9421 ctcctccttc cgacgtggta tccctgtgtc tccatgctgc ccccattcct aagcacatga
     9481 gttggtctaa tccctttctc cattggtcac ctttaactct ccatggcagg ctcactttaa
     9541 tcccttacac cgtgtccttc ccttcttgtg agttgtttgt cattaaccga cgttgtcagc
     9601 gacagacgtg tatctgaggg tgtcgtgcac gaccttcagc agggacttct gggccatgga
     9661 ggactgtcta ataaatacat ggacttataa actgactgca tgagcaatga aaaggccaaa
     9721 ttattctgat tttttttgaa tcactgtaaa aaaaaacatt tcttttgtat agagaacact
     9781 aaacatataa taaaagttgt tcaaaatgga
//
