LOCUS       NM_001107417            3035 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus neuropilin and tolloid like 2 (Neto2), mRNA.
ACCESSION   NM_001107417 XM_001068307 XM_039097725 XM_226349
VERSION     NM_001107417.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Guo,R., Xue,J., Shao,P., Cai,C. and Wang,Y.
  TITLE     NETO2-GluK2 interaction contributes to postoperative pain
            hypersensitivity through inducing PKCgamma activation and synaptic
            incorporation of AMPA receptor GluR1 subunits in rat dorsal horn
  JOURNAL   Neurosci Lett 813, 137430 (2023)
   PUBMED   37544581
  REMARK    GeneRIF: NETO2-GluK2 interaction contributes to postoperative pain
            hypersensitivity through inducing PKCgamma activation and synaptic
            incorporation of AMPA receptor GluR1 subunits in rat dorsal horn.
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Li,Y.J., Duan,G.F., Sun,J.H., Wu,D., Ye,C., Zang,Y.Y., Chen,G.Q.,
            Shi,Y.Y., Wang,J., Zhang,W. and Shi,Y.S.
  TITLE     Neto proteins regulate gating of the kainate-type glutamate
            receptor GluK2 through two binding sites
  JOURNAL   J Biol Chem 294 (47), 17889-17902 (2019)
   PUBMED   31628192
  REMARK    GeneRIF: Neto1 and Neto2 differentially regulate GluK2 fast gating
            kinetics
REFERENCE   3  (bases 1 to 3035)
  AUTHORS   Lomash,R.M., Sheng,N., Li,Y., Nicoll,R.A. and Roche,K.W.
  TITLE     Phosphorylation of the kainate receptor (KAR) auxiliary subunit
            Neto2 at serine 409 regulates synaptic targeting of the KAR subunit
            GluK1
  JOURNAL   J Biol Chem 292 (37), 15369-15377 (2017)
   PUBMED   28717010
  REMARK    GeneRIF: Data suggest Camk2a and Prkaca exhibit substrate
            specificity that includes phosphorylation of Neto2 at Ser409 in
            C-terminal domain; Neto2 Ser409 phosphorylation inhibits synaptic
            targeting of Gluk1. (Camk2a = calcium-calmodulin-dependent protein
            kinase II, alpha subunit; Prkaca = cAMP-dependent protein kinase,
            alpha-catalytic subunit; Neto2 = neuropilin and tolloid-like
            protein 2; Gluk1 = kainate receptor Gluk1)
REFERENCE   4  (bases 1 to 3035)
  AUTHORS   Sheng,N., Shi,Y.S. and Nicoll,R.A.
  TITLE     Amino-terminal domains of kainate receptors determine the
            differential dependence on Neto auxiliary subunits for trafficking
  JOURNAL   Proc Natl Acad Sci U S A 114 (5), 1159-1164 (2017)
   PUBMED   28100490
  REMARK    GeneRIF: GluK2 surface and synaptic expression are independent on
            the auxiliary subunits Neto1 and Neto2. In the CA1 hippocampal
            pyramidal cell, GluK2, in contrast to GluK1, traffics to the
            neuronal surface and synapse efficiently and the auxiliary subunits
            rNeto1 and mNeto2 caused no further enhancement of these two
            trafficking processes.
REFERENCE   5  (bases 1 to 3035)
  AUTHORS   Sheng,N., Shi,Y.S., Lomash,R.M., Roche,K.W. and Nicoll,R.A.
  TITLE     Neto auxiliary proteins control both the trafficking and
            biophysical properties of the kainate receptor GluK1
  JOURNAL   Elife 4, e11682 (2015)
   PUBMED   26720915
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3035)
  AUTHORS   Tang,M., Pelkey,K.A., Ng,D., Ivakine,E., McBain,C.J., Salter,M.W.
            and McInnes,R.R.
  TITLE     Neto1 is an auxiliary subunit of native synaptic kainate receptors
  JOURNAL   J Neurosci 31 (27), 10009-10018 (2011)
   PUBMED   21734292
REFERENCE   7  (bases 1 to 3035)
  AUTHORS   Copits,B.A., Robbins,J.S., Frausto,S. and Swanson,G.T.
  TITLE     Synaptic targeting and functional modulation of GluK1 kainate
            receptors by the auxiliary neuropilin and tolloid-like (NETO)
            proteins
  JOURNAL   J Neurosci 31 (20), 7334-7340 (2011)
   PUBMED   21593317
  REMARK    GeneRIF: NETO2 slows the desensitization rate of glutamate K2
            kainate (GluK2a) receptors, promotes plasma membrane localization
            of receptors in hippocampal neurons, and targets GluK1-containing
            receptors to synapses.
REFERENCE   8  (bases 1 to 3035)
  AUTHORS   Zhang,W., St-Gelais,F., Grabner,C.P., Trinidad,J.C., Sumioka,A.,
            Morimoto-Tomita,M., Kim,K.S., Straub,C., Burlingame,A.L., Howe,J.R.
            and Tomita,S.
  TITLE     A transmembrane accessory subunit that modulates kainate-type
            glutamate receptors
  JOURNAL   Neuron 61 (3), 385-396 (2009)
   PUBMED   19217376
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On or before Mar 9, 2021 this sequence version replaced
            XM_039097725.1, NM_001107417.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ149762.1, SRR26643429.55356.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               JAXUCZ010000019.1  37517555-37517841
            288-344             JAXUCZ010000019.1  37551505-37551561
            345-485             JAXUCZ010000019.1  37555525-37555665
            486-734             JAXUCZ010000019.1  37556276-37556524
            735-779             JAXUCZ010000019.1  37562097-37562141
            780-907             JAXUCZ010000019.1  37562222-37562349
            908-1136            JAXUCZ010000019.1  37581293-37581521
            1137-1250           JAXUCZ010000019.1  37585596-37585709
            1251-3035           JAXUCZ010000019.1  37586971-37588755
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19p11"
     gene            1..3035
                     /gene="Neto2"
                     /note="neuropilin and tolloid like 2"
                     /db_xref="GeneID:307757"
                     /db_xref="RGD:1305310"
     exon            1..287
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     CDS             254..1831
                     /gene="Neto2"
                     /note="neuropilin and tolloid-like protein 2;
                     brain-specific transmembrane protein containing 2 CUB and
                     1 LDL-receptor class A domains protein 2; neuropilin (NRP)
                     and tolloid (TLL)-like 2"
                     /codon_start=1
                     /product="neuropilin and tolloid-like protein 2 precursor"
                     /protein_id="NP_001100887.2"
                     /db_xref="GeneID:307757"
                     /db_xref="RGD:1305310"
                     /translation="MALEQLCAVLKVLLITVLVVEGIAVAQKTQDGQNIGIKHVPATQ
                     CGIWVRTSNGGHFASPNYPDSYPPNKECIYILEAAPRQRIELTFDERYYIEPSFECRF
                     DHLEVRDGPFGFSPLIDRYCGMKSPALIRSTGRFMWIKFSSDEELEGLGFRAKYSFIP
                     DPDFTYLGGILNPIPDCQFELSGADGIVRSSQVEQEEKTKPGQAVDCIWTIKATPKAK
                     IYLRFLDYQMEHSNECKRNFVAVYDGSSAIENLKAKFCSTVANDVMLKTGVGVIRMWA
                     DEGSRLSRFRMLFTSFVEPPCTSSTFFCHSNMCINNSLVCNGVQNCAYPWDENHCKEK
                     KKAGLFEQITKTHGTIIGVTSGIVLVLLIISILVQVKQPRKKVMACKTAFNKTGFQEV
                     FDPPHYELFSLREKEISADLADLSEELDNYQKLRRSSTASRCIHDHHCGSQASSVKQS
                     RTNLSSMELPFRNDFAQPQPMKTFNSTFKKSSYTFKQTHDCPEQALEDRVMEEIPCEI
                     YVRGRDDSAQASISIDF"
     exon            288..344
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            345..485
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            486..734
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            735..779
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            780..907
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1136
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1250
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..3035
                     /gene="Neto2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcttaaatga gccggggctc ccggcgcgcg ccacttcaag ggagcccgcc gcaggagcgg
       61 ccaccgccgg ctttcctcag cgcgccggga ggcggcggcc gtcggggacc ggacgcggcg
      121 agctgaggcg ctgcgctcgc tgcaggggag cggcggcggc cggcgatggg gaccgaacgg
      181 agctaccgtg ccgggccgtg accgccgcgc cgccgccgcc tcgggcgctc tcggagcgcc
      241 gacgctgagg aacatggccc tggagcagct ctgcgcggtc ctcaaagtgt tgctaataac
      301 ggtactcgta gtggaaggga ttgctgtggc ccaaaagacc caagatggac aaaatattgg
      361 aatcaagcat gttcctgcaa cccagtgtgg catttgggtt cgaaccagca acggaggtca
      421 ttttgcttcc ccaaattatc ctgactccta tccgccaaac aaggagtgta tctatatttt
      481 ggaagctgct cctcgtcaac ggatagagct gacctttgat gaacgatatt atatagagcc
      541 atcatttgaa tgccggtttg atcacttgga agttcgagac gggccatttg gcttctctcc
      601 tcttatagat cgttactgtg gcatgaaaag ccctgcatta attaggtcaa caggaagatt
      661 catgtggatt aaatttagtt ctgatgaaga acttgaagga ctaggatttc gagcaaaata
      721 ctcattcatc ccagatccag actttactta ccttggaggt attttaaatc ccattccaga
      781 ttgccagttt gagctctcgg gagctgatgg aatagtgcgg tctagccaag tagaacagga
      841 ggaaaaaaca aagcccggcc aagcggttga ctgcatatgg acaattaaag ctaccccgaa
      901 agctaagatt tacttgaggt tcttagatta tcaaatggaa cactcaaatg aatgcaaaag
      961 aaattttgtg gcagtctatg atgggagcag cgctatcgaa aatctgaagg ccaagttctg
     1021 cagcaccgtg gctaacgacg taatgttgaa aacgggagtg ggggtgatcc ggatgtgggc
     1081 agacgaaggc agtcggctga gcaggttcag gatgctcttc acttcctttg tggagcctcc
     1141 ctgtacaagc agcactttct tttgccatag caacatgtgc atcaacaatt cattagtctg
     1201 taatggcgtt caaaactgtg catacccttg ggatgagaac cactgtaaag aaaagaaaaa
     1261 agccggactg tttgaacaaa tcacgaagac tcatgggacg atcattggtg tcacatcagg
     1321 gatcgtcctg gtccttctca ttatttctat tttagtacaa gtgaaacagc cccggaaaaa
     1381 ggtcatggct tgcaaaactg catttaataa aactgggttc caggaagtat ttgatcctcc
     1441 tcattatgaa ctattttcac taagagagaa agagatttct gcagatctgg cagatttgtc
     1501 agaagaactt gacaactacc agaagctgcg acgctcctcc acggcctctc ggtgcatcca
     1561 tgaccaccac tgtgggtctc aagcatccag tgtcaaacaa agcaggacca acctcagttc
     1621 catggaactt cccttccgaa atgattttgc acaaccacag ccaatgaaaa catttaatag
     1681 caccttcaaa aaaagcagtt acactttcaa acaaacccat gactgccctg aacaggctct
     1741 agaggacaga gtgatggagg agattccctg tgaaatctat gtcagagggc gagatgactc
     1801 tgcacaagcg tccatatcca tcgactttta gttgttgact aaaggcactg ttaggatgtg
     1861 tgtgcagccc acgccaacac ccgttctgtc tccgcccgcg tcccatcttg tcctatcaaa
     1921 gttagaaagc ccttcatcat ttatcataat gctgacgatt tgatctcagg caatctatat
     1981 atgtggaacc aactaagaaa caatgtattc ggtggaaaga agtcatctgt tgctggtatc
     2041 ttaccgacag acaccaccag cccaatagca gttgaggagc aagtggtcac gttgagtcag
     2101 gtggagcagc atggtggtgt taacatcggg tactgtctgt gttcacacag ccccatcaac
     2161 ttgatctcac agtaaattca aacattttct ttatattcct ttgtcctcct tttttctgtt
     2221 ctgctttatt tgcctgtagt tttaccattc cagtgtcctg tgaaagggga taatatgtca
     2281 cttaactcag catgggcatg gtttgctcta aatgtgtaaa atatggctag gactgtcatg
     2341 agtggcagtt taacacacag tcatatttta tttcttcctg actgatttct acactgttgt
     2401 ctttaataac tgcaccttta gttctgcttt tctgtgggaa gtagtctatt acatgattta
     2461 gcacctttaa atcatgtttc gaattagtta tgctacaaaa gtaataatcc tggggtacat
     2521 tgttaccaga tacacatgag tggaaatttg agaaggatta aaaacttgta gcaacctctg
     2581 aagttttccc cattgggtct cttcagtgac agaaattcac cccagtaatc agggtcctgg
     2641 aattcactgg tgtgcagacc tttaactgtg gctccatgca gagcctctct gatcttgttc
     2701 cctctcatct ttcaaagatt tctttgttct cttggaattg gtttgtcttt gacacatggc
     2761 catggttggt ggaagcttaa agaaattctc cgagaaactg tgtgagcctt tacatagctt
     2821 ccgcttgaga cagtgcattt ttctacccat tgtcaagaag agcaacttca acttttttta
     2881 agctgcactt ttcaccttat gttttgtaaa gtatataaaa caataattta taaacatatc
     2941 aaaactcatt gtgtttttta gacttttgat attatttgat attgtataaa ctttattaga
     3001 tccagattta agacataaaa gacaaatttc tttaa
//
