LOCUS       NM_031069               3388 bp    mRNA    linear   ROD 16-FEB-2024
DEFINITION  Rattus norvegicus neural EGFL like 1 (Nell1), mRNA.
ACCESSION   NM_031069
VERSION     NM_031069.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wu,J., Wang,Q., Han,Q., Zhu,H., Li,M., Fang,Y. and Wang,X.
  TITLE     Effects of Nel-like molecule-1 and bone morphogenetic protein 2
            combination on rat pulp repair
  JOURNAL   J Mol Histol 50 (3), 253-261 (2019)
   PUBMED   30937700
  REMARK    GeneRIF: These findings suggested that combined use of NELL-1 and
            BMP2 could positively regulate pulp repair.
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Han,Q., Wang,Q., Wu,J., Li,M., Fang,Y., Zhu,H. and Wang,X.
  TITLE     Nell-1 promotes the neural-like differentiation of dental pulp
            cells
  JOURNAL   Biochem Biophys Res Commun 513 (2), 515-521 (2019)
   PUBMED   30979495
  REMARK    GeneRIF: The expression levels of neural markers were increased in
            rat pulp and in cultured human dental pulp stem cells stimulated
            with Nell-1 protein. This is the first study to demonstrate the
            effect of Nell-1 on dental pulp cells neural-like differentiation.
REFERENCE   3  (bases 1 to 3388)
  AUTHORS   Shen,J., James,A.W., Zhang,X., Pang,S., Zara,J.N., Asatrian,G.,
            Chiang,M., Lee,M., Khadarian,K., Nguyen,A., Lee,K.S., Siu,R.K.,
            Tetradis,S., Ting,K. and Soo,C.
  TITLE     Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis
            and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2
  JOURNAL   Am J Pathol 186 (2), 419-434 (2016)
   PUBMED   26772960
  REMARK    GeneRIF: establish the feasibility of combining NELL-1 with BMP2 to
            improve clinical bone regeneration and provide mechanistic insight
            into canonical Wnt pathway activity during NELL-1 and BMP2
            osteogenesis
REFERENCE   4  (bases 1 to 3388)
  AUTHORS   Pang,S., Shen,J., Liu,Y., Chen,F., Zheng,Z., James,A.W., Hsu,C.Y.,
            Zhang,H., Lee,K.S., Wang,C., Li,C., Chen,X., Jia,H., Zhang,X.,
            Soo,C. and Ting,K.
  TITLE     Proliferation and osteogenic differentiation of mesenchymal stem
            cells induced by a short isoform of NELL-1
  JOURNAL   Stem Cells 33 (3), 904-915 (2015)
   PUBMED   25376942
  REMARK    GeneRIF: NELL-1570 has the potential to be used for cell-based or
            hormone-based therapy of bone regeneration.
REFERENCE   5  (bases 1 to 3388)
  AUTHORS   Kwak,J., Zara,J.N., Chiang,M., Ngo,R., Shen,J., James,A.W.,
            Le,K.M., Moon,C., Zhang,X., Gou,Z., Ting,K. and Soo,C.
  TITLE     NELL-1 injection maintains long-bone quantity and quality in an
            ovariectomy-induced osteoporotic senile rat model
  JOURNAL   Tissue Eng Part A 19 (3-4), 426-436 (2013)
   PUBMED   23083222
  REMARK    GeneRIF: Thus, NELL-1 effectively enhances in situ osteogenesis in
            the bone marrow, making it potentially useful in the prevention and
            treatment of osteoporotic fractures.
REFERENCE   6  (bases 1 to 3388)
  AUTHORS   Cowan,C.M., Cheng,S., Ting,K., Soo,C., Walder,B., Wu,B., Kuroda,S.
            and Zhang,X.
  TITLE     Nell-1 induced bone formation within the distracted intermaxillary
            suture
  JOURNAL   Bone 38 (1), 48-58 (2006)
   PUBMED   16243593
  REMARK    GeneRIF: Nell1 accelerates chondrocyte hypertrophy and endochondral
            bone formation.
REFERENCE   7  (bases 1 to 3388)
  AUTHORS   Zhang,X., Carpenter,D., Bokui,N., Soo,C., Miao,S., Truong,T.,
            Wu,B., Chen,I., Vastardis,H., Tanizawa,K., Kuroda,S. and Ting,K.
  TITLE     Overexpression of Nell-1, a craniosynostosis-associated gene,
            induces apoptosis in osteoblasts during craniofacial development
  JOURNAL   J Bone Miner Res 18 (12), 2126-2134 (2003)
   PUBMED   14672347
  REMARK    GeneRIF: Nell-1 overexpression disrupts these pathways resulting in
            craniofacial anomalies such as premature suture closure.
REFERENCE   8  (bases 1 to 3388)
  AUTHORS   Zhang,X., Kuroda,S., Carpenter,D., Nishimura,I., Soo,C., Moats,R.,
            Iida,K., Wisner,E., Hu,F.Y., Miao,S., Beanes,S., Dang,C.,
            Vastardis,H., Longaker,M., Tanizawa,K., Kanayama,N., Saito,N. and
            Ting,K.
  TITLE     Craniosynostosis in transgenic mice overexpressing Nell-1
  JOURNAL   J Clin Invest 110 (6), 861-870 (2002)
   PUBMED   12235118
  REMARK    GeneRIF: Data show that Nell-1 overexpression induced calvarial
            overgrowth resulting in premature suture closure
            Erratum:[J Clin Invest 2002 Nov;110(10):1573]
REFERENCE   9  (bases 1 to 3388)
  AUTHORS   Kuroda,S. and Tanizawa,K.
  TITLE     Involvement of epidermal growth factor-like domain of NELL proteins
            in the novel protein-protein interaction with protein kinase C
  JOURNAL   Biochem Biophys Res Commun 265 (3), 752-757 (1999)
   PUBMED   10600492
REFERENCE   10 (bases 1 to 3388)
  AUTHORS   Kuroda,S., Oyasu,M., Kawakami,M., Kanayama,N., Tanizawa,K.,
            Saito,N., Abe,T., Matsuhashi,S. and Ting,K.
  TITLE     Biochemical characterization and expression analysis of neural
            thrombospondin-1-like proteins NELL1 and NELL2
  JOURNAL   Biochem Biophys Res Commun 265 (1), 79-86 (1999)
   PUBMED   10548494
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000033.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_031069.3.
            
            On Mar 8, 2021 this sequence version replaced NM_031069.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U48246.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760396
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-600               JACYVU010000033.1  23586260-23586859
            601-729             JACYVU010000033.1  23595460-23595588
            730-880             JACYVU010000033.1  23676981-23677131
            881-1051            JACYVU010000033.1  23731150-23731320
            1052-1148           JACYVU010000033.1  23769635-23769731
            1149-1221           JACYVU010000033.1  23817657-23817729
            1222-1304           JACYVU010000033.1  23818376-23818458
            1305-1439           JACYVU010000033.1  23828087-23828221
            1440-1542           JACYVU010000033.1  23829318-23829420
            1543-1616           JACYVU010000033.1  23840998-23841071
            1617-1716           JACYVU010000033.1  23851824-23851923
            1717-1845           JACYVU010000033.1  23870890-23871018
            1846-1971           JACYVU010000033.1  24035611-24035736
            1972-2094           JACYVU010000033.1  24142410-24142532
            2095-2190           JACYVU010000033.1  24256224-24256319
            2191-2331           JACYVU010000033.1  24413203-24413343
            2332-2525           JACYVU010000033.1  24434113-24434306
            2526-2702           JACYVU010000033.1  24441067-24441243
            2703-2927           JACYVU010000033.1  24443217-24443441
            2928-3388           JACYVU010000033.1  24450366-24450826
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..3388
                     /gene="Nell1"
                     /note="neural EGFL like 1"
                     /db_xref="GeneID:81733"
                     /db_xref="RGD:620998"
     exon            1..600
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     CDS             546..2978
                     /gene="Nell1"
                     /note="NEL-like protein 1; protein kinase C-binding
                     protein NELL1; NEL-like 1"
                     /codon_start=1
                     /product="protein kinase C-binding protein NELL1
                     precursor"
                     /protein_id="NP_112331.2"
                     /db_xref="GeneID:81733"
                     /db_xref="RGD:620998"
                     /translation="MPMDVILVLWFCVCTARTVLGFGMDPDLQLDIISELDLVNTTLG
                     VTQVAGLHNASKAFLFQDVQREIHSAPHVSEKLIQLFRNKSEFTFLATVQQKPSTSGV
                     ILSIRELEHSYFELESSGPREEIRYHYIHGGKPRTEALPYRMADGQWHKVALSVSASH
                     LLLHIDCNRIYERVIDPPETNLPPGSNLWLGQRNQKHGFFKGIIQDGKIIFMPNGFIT
                     QCPNLNRTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLGQLENCHCEKTCQVS
                     GLLYRDQDSWVDGDNCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHISGQCCKVCRPK
                     CIYGGKVLAEGQRILTKTCRECRGGVLVKITEACPPLNCSAKDHILPENQCCRVCPGH
                     NFCAEAPKCGENSECKNWNTKATCECKNGYISVQGNSAYCEDIDECAAKMHYCHANTV
                     CVNLPGLYRCDCVPGYIRVDDFSCTEHDDCGSGQHNCDKNAICTNTVQGHSCTCQPGY
                     VGNGTICKAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECAEGFVECHNHSRC
                     VNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLC
                     PSGPSCSGDCPHEGGLKHNGQVWILREDRCSVCSCKDGKIFCRRTACDCQNPNVDLFC
                     CPECDTRVTSQCLDQSGQKLYRSGDNWTHSCQQCRCLEGEADCWPLACPSLGCEYTAM
                     FEGECCPRCVSDPCLADNIAYDIRKTCLDSFGVSRLSGAVWTMAGSPCTTCKCKNGRV
                     CCSVDLECIENN"
     exon            601..729
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            730..880
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1051
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1148
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1221
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1304
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1439
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1542
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1616
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1716
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1845
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1971
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2094
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2190
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2331
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2525
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            2526..2702
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2927
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
     exon            2928..3388
                     /gene="Nell1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tccccaaaga gggtctggga gcactcggtg acaccgccta ggtctggttt ccctaggaaa
       61 caacccacgc accagggttg ggctgcttcg gacacggccc ggatgaactg ttgcaacttg
      121 ggcagcccgc agcgcggcgc agtggcagag gcgggggcgt gaagccggga ggcgcgtgta
      181 accaggatcc tcgaagctcc ggagacccgg aggcgaaagg atccgcacag gaacccaggg
      241 caacctgcgc gagtggcgga gccccgcgct cccgccctag ctcaagggga gcgcagtcgt
      301 agcggggttc agagccccag ccccgtgcca ccgccacccg cggtgggggg tgggctgggg
      361 gcggggcagc tggggttgcc ttcccgggcg cgatccccga gcgcggcacc cggcgccgcc
      421 gagccacctc ccctgccgcc cgccagcaag tttggcggct tcgagcccag agagccacgg
      481 ctttctgaag cactggtttc ttgttagcgt tggtgcgccc tgcttggcgg gggttctccg
      541 gagcgatgcc gatggatgtg attttagttt tgtggttctg tgtatgcacc gccaggacag
      601 tgttgggctt tgggatggac cctgaccttc agctggacat catctcagag ctcgacctgg
      661 tgaacaccac cctgggagtc acgcaggtgg ctggactgca caacgccagt aaagcatttc
      721 tatttcaaga tgtacagaga gagatccatt cggcccctca cgtgagtgag aagctgatcc
      781 agctattccg gaataagagc gagttcacct ttttggctac agtgcagcag aaaccatcca
      841 cctcaggggt gatactgtcc atccgggagc tggagcacag ctattttgaa ctggagagca
      901 gtggcccaag agaagagata cgctaccatt acatacatgg tggaaagccc aggactgagg
      961 cccttcccta ccgcatggca gacggacaat ggcacaaggt cgcgctgtca gtgagcgcct
     1021 ctcacctcct gctccacatc gactgcaata ggatttacga gcgtgtgata gaccctccgg
     1081 agaccaacct tcctccagga agcaatctgt ggcttgggca acgtaaccaa aagcatggct
     1141 ttttcaaagg aatcatccaa gatggtaaga tcatcttcat gccgaatggt ttcatcacac
     1201 agtgtcccaa cctcaatcgc acttgcccaa catgcagtga cttcctgagc ctggttcaag
     1261 gaataatgga tttgcaagag cttttggcca agatgactgc aaaactgaat tatgcagaga
     1321 cgagacttgg tcaactggaa aattgccact gtgagaagac ctgccaagtg agtgggctgc
     1381 tctacaggga ccaagactcc tgggtggatg gtgacaactg taggaactgc acgtgcaaaa
     1441 gtggtgccgt ggagtgccgc aggatgtcct gtcccccgct caactgttcc ccagactcac
     1501 ttcctgtgca catttccggc cagtgttgta aagtttgcag accaaaatgt atctatggag
     1561 gaaaagttct tgctgagggc cagcggattt taaccaagac ctgccgggaa tgtcgaggtg
     1621 gagtcttggt aaaaatcaca gaagcttgcc ctcctttgaa ctgctcagca aaggatcata
     1681 ttcttccaga gaatcagtgc tgcagggtct gcccaggtca taacttctgt gcagaagcac
     1741 ctaagtgcgg agaaaactcg gaatgcaaaa attggaatac aaaagcaacc tgtgagtgca
     1801 agaatggata catctctgtc cagggcaact ctgcatactg tgaagatatt gatgagtgtg
     1861 cagctaaaat gcactattgt catgccaaca ccgtgtgtgt caacttgccg gggttgtatc
     1921 gctgtgactg cgtcccagga tacatccgtg tggatgactt ctcttgtacg gagcatgatg
     1981 attgtggcag cggacaacac aactgcgaca aaaatgccat ctgtaccaac acagtccagg
     2041 gacacagctg cacctgccag ccgggttacg tgggaaatgg caccatctgc aaagcattct
     2101 gtgaagaggg ttgcagatac ggaggtacct gtgtggctcc taacaagtgt gtctgtcctt
     2161 ctggattcac gggaagccac tgtgagaaag atattgatga atgcgcagag ggattcgttg
     2221 aatgccacaa ccactcccgc tgtgttaacc tgccagggtg gtaccactgt gagtgcagaa
     2281 gcggtttcca tgacgatggg acctactcac tgtccgggga gtcctgcatt gatatcgatg
     2341 aatgtgcctt aagaactcac acttgttgga atgactctgc ctgcatcaac ttagcaggag
     2401 gatttgactg cctgtgtccc tctgggccct cctgctctgg tgactgtccc cacgaaggag
     2461 ggctgaagca taatgggcag gtgtggattc tgagagaaga caggtgttca gtctgttcct
     2521 gcaaggatgg gaagatattc tgccggcgga cagcttgtga ttgccagaat ccaaatgttg
     2581 accttttttg ctgcccagag tgcgatacca gggtcaccag ccaatgttta gatcaaagtg
     2641 gacagaagct ctatcgaagt ggagacaact ggacccacag ctgccagcag tgccgatgtc
     2701 tggaaggaga ggcagactgc tggcctctgg cttgccctag tttgggctgt gaatacacag
     2761 ccatgtttga aggggagtgt tgtccccgat gtgtcagtga cccctgcctg gctgataata
     2821 ttgcctatga catcagaaaa acttgcctgg acagctttgg tgtttcgagg ctgagcggag
     2881 ccgtgtggac aatggctgga tctccttgta caacctgcaa atgcaagaat gggagagtct
     2941 gctgctctgt ggatctggag tgtattgaga ataactgaag attttaaatg gactcgtcac
     3001 gtgagaaaat ggacaaaatg atcatcccac ctgaggaaga agaggggctg atttcttttt
     3061 ctttttaacc acagtcaatt accaaagtct ccatctgagg aaggcgtttg gattgccttt
     3121 gccactttgc tcatccttgc tgacctagtc tagatgcctg cagtaccgtg catttcggtc
     3181 gatggttgtt gagtctcagt gttgtaaatc gcatttccct cgtcagatca tttacagata
     3241 catttaaagg ggttccatga taaatgttaa tgtaactttt gtttattttg tgtactgaca
     3301 taatagagac ttggcaccat ttatttattt ttcttgattt ttggatcaaa ttctaaaaat
     3361 aaagttgcct gttgtgactt tcgtctca
//
