LOCUS       NM_001008339            2215 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus negative elongation factor complex member A
            (Nelfa), mRNA.
ACCESSION   NM_001008339 XM_223539
VERSION     NM_001008339.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Luo M, Lu X, Zhu R, Zhang Z, Chow CC, Li R and Simons SS Jr.
  TITLE     A conserved protein motif is required for full modulatory activity
            of negative elongation factor subunits NELF-A and NELF-B in
            modifying glucocorticoid receptor-regulated gene induction
            properties
  JOURNAL   J Biol Chem 288 (47), 34055-34072 (2013)
   PUBMED   24097989
  REMARK    GeneRIF: These studies allow us to position the actions of two new
            modulators of GR-regulated transactivation, NELF-A and NELF-B,
            relative to other factors in the overall gene induction sequence.
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Kwak H and Lis JT.
  TITLE     Control of transcriptional elongation
  JOURNAL   Annu Rev Genet 47, 483-508 (2013)
   PUBMED   24050178
  REMARK    Review article
REFERENCE   3  (bases 1 to 2215)
  AUTHORS   Flynn RA, Almada AE, Zamudio JR and Sharp PA.
  TITLE     Antisense RNA polymerase II divergent transcripts are P-TEFb
            dependent and substrates for the RNA exosome
  JOURNAL   Proc Natl Acad Sci U S A 108 (26), 10460-10465 (2011)
   PUBMED   21670248
REFERENCE   4  (bases 1 to 2215)
  AUTHORS   Saha RN, Wissink EM, Bailey ER, Zhao M, Fargo DC, Hwang JY, Daigle
            KR, Fenn JD, Adelman K and Dudek SM.
  TITLE     Rapid activity-induced transcription of Arc and other IEGs relies
            on poised RNA polymerase II
  JOURNAL   Nat Neurosci 14 (7), 848-856 (2011)
   PUBMED   21623364
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2215)
  AUTHORS   Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao
            Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS,
            Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M,
            Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN,
            Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D and Ko MS.
  TITLE     Uncovering early response of gene regulatory networks in ESCs by
            systematic induction of transcription factors
  JOURNAL   Cell Stem Cell 5 (4), 420-433 (2009)
   PUBMED   19796622
REFERENCE   6  (bases 1 to 2215)
  AUTHORS   Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim
            DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T and Handa H.
  TITLE     Human transcription elongation factor NELF: identification of novel
            subunits and reconstitution of the functionally active complex
  JOURNAL   Mol Cell Biol 23 (6), 1863-1873 (2003)
   PUBMED   12612062
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC085948.1.
            
            On Dec 16, 2004 this sequence version replaced XM_223539.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085948.1, SRR8487230.65514.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     gene            1..2215
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /note="negative elongation factor complex member A"
                     /db_xref="GeneID:305455"
                     /db_xref="RGD:1305556"
     exon            1..269
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1652
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /note="Wolf-Hirschhorn syndrome candidate 2 protein"
                     /codon_start=1
                     /product="negative elongation factor A"
                     /protein_id="NP_001008340.1"
                     /db_xref="GeneID:305455"
                     /db_xref="RGD:1305556"
                     /translation="MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLC
                     FHRLSSAVKLKLLLGTLHLPRRTVDEMKGALMDIIQLATLDSDPWVLMVADILKSFPD
                     TGSLNLDLEEQNPNVQDILGELREKVSECEASAMLPLECQYLNKNALTTLAGPLTPPV
                     KHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIP
                     KQAPFRSPTTPSVFSPSGNRTPIPPSRTPLQKERGVKLLDISELNTVGAGREAKRRRK
                     TLDTEVVEKPTKEETVVENATPDYAAGLVSTQKLGSLNSEPTLPSTSYLPSTPSVVPA
                     SSYIPSSETPPAPPSREASRPPEEPSAPSPTLPTQFKQRAPMYNSGLSPATPTPAAPA
                     SPLTPTAPPAVTPTAQTPPVAMVAPQTQAPAPVQQQPKKNLSLTREQMFAAQEMFKTA
                     NKVTRPEKALILGFMAGSRENPCPEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVF
                     EMNYATGQWTRFKKYKPMTNVS"
     exon            270..441
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            442..603
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            604..693
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            694..824
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..894
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            895..983
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1095
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1367
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1467
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..2192
                     /gene="Nelfa"
                     /gene_synonym="Whsc2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctggggaca cgtaggcgtc cgcgtaagat gccggccgac gcgcgctgcc gccgccaaga
       61 tggcttccat gcgggagagc gacacgggcc tgtggctgca caacaaactg ggcgccacgg
      121 acgagctgtg ggcgccaccc agcatcgcgt ccctgctcac cgctgcggtc atcgataaca
      181 tccgcctctg cttccaccgc ctctcatcgg ctgtgaagct caagctgctg ctcgggacgc
      241 tgcatcttcc gcgccgcacg gtggatgaga tgaagggcgc tctgatggat atcatccagc
      301 tggccactct ggactcagat ccctgggtcc tcatggttgc tgacattctg aagtccttcc
      361 ctgacacagg ctccttgaat ctggaccttg aggaacagaa ccccaatgtt caagacatct
      421 taggagaact tagagaaaaa gtgagtgagt gcgaggcatc ggccatgcta ccgttggagt
      481 gccagtacct gaacaagaat gccctgacca cccttgctgg tcccctcacc ccgccagtga
      541 aacattttca gctgaagcgg aaacctaaga gtgccacact gcgggcagaa ctgctacaga
      601 aatctaccga gacagcccag cagctgaaga ggagtgctgg ggtcccattc cacgccaagg
      661 gccgggggct gctccgaaag atggacacta caacccctct caaaggcatc ccgaagcaag
      721 cccccttcag aagtcccacc acacccagtg tcttcagccc ttctgggaac cgcactccaa
      781 taccaccttc aaggacgcca ctgcagaaag aaaggggtgt gaagctgctg gatatttctg
      841 agctgaatac agttggtgct ggccgagagg caaagaggag gaggaagact ttagacacag
      901 aagtggtaga aaagcccacc aaggaagaga cggttgttga aaatgccacc cctgactatg
      961 ctgctggcct ggtgtccaca cagaaacttg ggtctctgaa cagtgagcca acactgccct
     1021 ccacaagtta cctgccctct acacccagtg tggtacctgc ctcatcctac atccccagct
     1081 ctgagactcc cccagctcca ccctcccggg aagccagccg gccacctgag gaacccagcg
     1141 ccccaagtcc cacactgcca acacagttta aacaaagggc acctatgtat aacagtggcc
     1201 tgagtccagc gaccccaaca cctgcagcac ctgcctcacc tctgacacct actgctcccc
     1261 cagctgtcac tcccactgct cagacacccc cagtggccat ggtggcccca cagacccagg
     1321 ccccggcccc tgttcagcag caacctaaga agaacctgtc cctcacgaga gaacagatgt
     1381 ttgctgccca ggagatgttc aagacagcaa acaaggtcac tcggccagag aaggccctca
     1441 tcctgggctt catggctggc tctcgagaaa acccatgccc ggagcaaggg gatgtgatcc
     1501 agatcaagct cagcgagcac acagaggacc tgcccaaggc agacggccag ggcagtacca
     1561 ccatgctagt ggacacggtg ttcgagatga actatgccac aggccagtgg acgcgcttca
     1621 agaagtacaa gcctatgacc aatgtgtcct aagtgcggtc tctgctgctg accgtcatcg
     1681 caccagagga caaattctgc tggttctgct gtttcctgag cagataccgg aaggatgtgt
     1741 tccacctagc actgctgtcc acaggctctg gggccccagc cagttctctg gggagtttag
     1801 gcttattttt ttatattgta atgagttagt tttttgtgtg attaagatac tttttttgga
     1861 ttcaatatta tgctttgaat gcaaaccaaa atttacttca taattttagt gcctgttaga
     1921 cttttacctt ttttacttct tcttaaaata gtactggcta agagggcttt ctttttctct
     1981 gcttgtgcat gcccttcctc atgaggttga ggggaccaaa ggtgacacct ggcagtctag
     2041 ggtgtgaggg cggctatgac cagccttgat gccactacct accagggatt tgtatgtctt
     2101 ttgtacagtt gtaaactttt caaacctgtg tctgagttcc tattttcatt gtaaaatgac
     2161 ctgagcatta aagtttagtt tttctaaaga aaaaaaaaaa aaaaaaaaaa aaaaa
//
