LOCUS       NM_001106747            5417 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus NIMA-related kinase 9 (Nek9), mRNA.
ACCESSION   NM_001106747 XM_001057566 XM_008764757 XM_216755
VERSION     NM_001106747.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Vaz Meirelles,G., Ferreira Lanza,D.C., da Silva,J.C., Santana
            Bernachi,J., Paes Leme,A.F. and Kobarg,J.
  TITLE     Characterization of hNek6 interactome reveals an important role for
            its short N-terminal domain and colocalization with proteins at the
            centrosome
  JOURNAL   J Proteome Res 9 (12), 6298-6316 (2010)
   PUBMED   20873783
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Rapley,J., Nicolas,M., Groen,A., Regue,L., Bertran,M.T.,
            Caelles,C., Avruch,J. and Roig,J.
  TITLE     The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a
            novel site necessary for mitotic spindle formation
  JOURNAL   J Cell Sci 121 (Pt 23), 3912-3921 (2008)
   PUBMED   19001501
REFERENCE   3  (bases 1 to 5417)
  AUTHORS   Cao,Z., Li,C., Higginbotham,J.N., Franklin,J.L., Tabb,D.L.,
            Graves-Deal,R., Hill,S., Cheek,K., Jerome,W.G., Lapierre,L.A.,
            Goldenring,J.R., Ham,A.J. and Coffey,R.J.
  TITLE     Use of fluorescence-activated vesicle sorting for isolation of
            Naked2-associated, basolaterally targeted exocytic vesicles for
            proteomics analysis
  JOURNAL   Mol Cell Proteomics 7 (9), 1651-1667 (2008)
   PUBMED   18504258
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On or before Nov 24, 2020 this sequence version replaced
            XM_008764757.1, NM_001106747.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360172.1244180.1,
                                           SRR26643284.9143.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-387               JAXUCZ010000006.1  110715200-110715586 c
            388-565             JAXUCZ010000006.1  110712534-110712711 c
            566-621             JAXUCZ010000006.1  110710617-110710672 c
            622-692             JAXUCZ010000006.1  110709987-110710057 c
            693-798             JAXUCZ010000006.1  110708505-110708610 c
            799-930             JAXUCZ010000006.1  110706229-110706360 c
            931-1041            JAXUCZ010000006.1  110704092-110704202 c
            1042-1106           JAXUCZ010000006.1  110702011-110702075 c
            1107-1157           JAXUCZ010000006.1  110698713-110698763 c
            1158-1350           JAXUCZ010000006.1  110697262-110697454 c
            1351-1495           JAXUCZ010000006.1  110693070-110693214 c
            1496-1696           JAXUCZ010000006.1  110692122-110692322 c
            1697-1743           JAXUCZ010000006.1  110691908-110691954 c
            1744-1899           JAXUCZ010000006.1  110690619-110690774 c
            1900-2008           JAXUCZ010000006.1  110689179-110689287 c
            2009-2170           JAXUCZ010000006.1  110688199-110688360 c
            2171-2341           JAXUCZ010000006.1  110686562-110686732 c
            2342-2401           JAXUCZ010000006.1  110685639-110685698 c
            2402-2610           JAXUCZ010000006.1  110683761-110683969 c
            2611-2772           JAXUCZ010000006.1  110680923-110681084 c
            2773-2979           JAXUCZ010000006.1  110679046-110679252 c
            2980-5417           JAXUCZ010000006.1  110675107-110677544 c
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q31"
     gene            1..5417
                     /gene="Nek9"
                     /note="NIMA-related kinase 9"
                     /db_xref="GeneID:299204"
                     /db_xref="RGD:1307187"
     exon            1..387
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     CDS             172..3102
                     /gene="Nek9"
                     /EC_number="2.7.11.1"
                     /note="NIMA (never in mitosis gene a)- related kinase 9"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek9"
                     /protein_id="NP_001100217.2"
                     /db_xref="GeneID:299204"
                     /db_xref="RGD:1307187"
                     /translation="MSVLGEYERHCDSINSDFGSESGGGGDSGSGPSASPGPRAGGAA
                     EQEELHYIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVIL
                     ALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIV
                     SAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMS
                     PELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQY
                     SLGLIQLVHACLDQDPERRPTADALLDLPLLRKRRREMEEKVTLLNAPTKRPRSSTVT
                     EAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTW
                     VNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIHQVSCGDDFTVCVTDEGQLYAFG
                     SDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKEVYSWGCGEY
                     GRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNEFNKLGLN
                     QCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNK
                     CGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLA
                     MTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLS
                     IGTVVQSSSPGGRIGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVG
                     NSCGASSSCPGWLRKELENAEFIPMPDSPTPLSAAFSQSEKDTLPYEELQGLKVASEV
                     PPEHQPAVGAWPPRLNPAVPCVGKALTSPACACSALQVEVERLQGLVLKCLDEQQKLQ
                     QENLQIVTQLQKLNKRLEGGQQVGMHSRGTQTAKEDMEMDPKPDLDSDSWCLLGTDSC
                     RPSL"
     exon            388..565
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            566..621
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            622..692
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            693..798
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            799..930
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1041
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1106
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1157
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1350
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1495
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1696
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1743
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1899
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2008
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2170
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2341
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2401
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2610
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2772
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2979
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
     exon            2980..5417
                     /gene="Nek9"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaataccgag aactgcttcc gggtgagggg gcaggagcca ccatggaagc tcactgggcc
       61 gcagtgttgc ctaagccggt ggcttacgtc tgaggccagt gaggcgctac gcggccggca
      121 actgcccgcc gtggatacgc aggcggctca cgccgagctg cggcctccgc catgtccgtg
      181 ctgggcgagt atgagcgaca ctgcgattcc atcaactcgg acttcggaag cgagtccggg
      241 ggtggcgggg actcgggctc ggggcccagc gctagcccgg ggccgcgagc cggcggtgcg
      301 gcggagcagg aggagctgca ctacatcccc atccgcgtcc tgggccgcgg cgcctttggg
      361 gaggccacgc tataccgccg caccgaggat gactcactgg tggtgtggaa ggaagttgat
      421 ttgacccgac tttctgagaa agagcgtcgt gacgccttga atgagattgt cattctagcg
      481 ctgctgcagc atgacaacat tattgcctac tacaaccact ttatggacaa tactaccctg
      541 ctgattgagt tggagtattg taacggaggg aacctctatg acaaaatcct tcgtcagaag
      601 gacaagttgt tcgaagaaga gatggtggtg tggtaccttt ttcagattgt ttcagcagtg
      661 agctgtatcc ataaagctgg aatcctccat agagatataa agacattaaa tatttttctg
      721 accaaggcaa atctgataaa gcttggagac tatggcctag caaagaaact taattctgag
      781 tactctatgg ctgaaacact tgtgggcact ccatattaca tgtctccaga gctctgccaa
      841 ggagtaaagt ataatttcaa gtctgatatc tgggcagttg gctgtgtcat ttttgaactg
      901 cttactctga aaagaacatt tgatgctaca aacccactca acctgtgtgt gaagattgtg
      961 cagggtatcc gggccatgga agtcgactct agtcagtatt ctttggggct catccagttg
     1021 gtgcatgcat gcctcgacca ggatcctgag cggagaccca ctgcagatgc acttctggat
     1081 ctcccccttc tcaggaaacg taggagagag atggaagaaa aagtcactct gcttaatgca
     1141 cctacaaaga gacccaggtc aagcactgtg actgaagcac ccattgctgt ggtaacatct
     1201 cgaaccagtg aagtctatgt ttggggtggt ggaaaatcca cacctcagaa attggatgtc
     1261 atcaagagtg gctgtagtgc tcgacaggtg tgcgcaggga acacccactt tgctgtggtc
     1321 accgtggaga aggaactata tacctgggtg aacatgcaag ggggcactaa acttcatggt
     1381 cagctaggac atggagacaa agcttcctac cgacagccaa agcatgtgga gaagttgcaa
     1441 ggaaaagcta tccaccaggt gtcatgtggt gatgacttca ctgtttgtgt gacagatgaa
     1501 ggtcagctct atgcctttgg atcagattat tatggctgca tgggagtgga caaggtttct
     1561 ggtcctgaag tgcttgaacc catgcagctg aacttcttcc tcagcaatcc agtggagcag
     1621 gtctcctgtg gggataatca tgtggtagtt ctgacacgaa acaaggaagt ctactcttgg
     1681 ggctgtggtg aatatggacg cctgggtttg gattcagaag aggattacta tacaccacag
     1741 agggtggatg ttccaaaggc cttaatcatt gttgcggttc aatgtggctg tgatgggacc
     1801 ttcctgctca ctcagtcagg caaagtgctg gcctgcggac tcaacgagtt caacaaactg
     1861 gggctgaatc agtgcatgtc tgggatcatc aaccatgaag cataccacga agttccctac
     1921 acgacctcct ttaccttggc caaacagtta tccttttaca aaatccgtac cattgctcca
     1981 ggcaagactc acacggctgc tattgatgag cgaggccggt tgctgacttt tggctgtaat
     2041 aagtgtgggc agctgggtgt tgggaattat aagaagcgcc tgggaatcaa cctgttgggg
     2101 ggaccccttg gtgggaagca agtgatcagg gtctcctgtg gcgatgagtt caccatcgct
     2161 gccaccgatg ataatcacat ttttgcctgg ggtaatggtg gtaacggccg cctggcaatg
     2221 actcccacag agagaccaca tggctctgat atctgtacct cctggcctcg gcctattttt
     2281 ggatctctgc atcatgtccc agacctttct tgccgtggct ggcacaccat tcttattgtt
     2341 gagaaagtat tgaattccaa gaccatccgt tctaacagca gtggcctatc catcggaact
     2401 gtggttcaga gctccagccc aggcggccgc attggtggtg gcgggggtga agaggaagat
     2461 agtcagcagg aatctgaaac tccagatcca agtggaggtt tccgaggaac aatggaagca
     2521 gaccgtggaa tggaaggttt aatcagcccc acagaggctg tgggaaacag ttgtggggct
     2581 agcagctcct gtcctggctg gcttcggaag gagctggaaa atgctgagtt catccccatg
     2641 cctgacagcc caactcccct aagtgcagca ttttcacaat ctgagaagga cactctaccc
     2701 tatgaagagc tgcaaggact caaagtcgca tctgaagtcc ctccggaaca ccagcctgca
     2761 gtaggagcct ggcccccccg gctgaatcct gcagtgccgt gtgttgggaa agcattgacc
     2821 tctcctgcct gtgcatgcag tgctctgcaa gtggaggttg agaggctgca gggcctggtg
     2881 ttaaagtgtc tggatgagca gcagaagctc cagcaagaaa acctccagat tgttactcag
     2941 ctccaaaagc tcaacaagag actggaagga ggccagcagg tggggatgca ctccagagga
     3001 acccagacag caaaggaaga catggaaatg gatccaaagc ctgacttaga ttcagattcc
     3061 tggtgcctcc ttggaacaga ctcttgtcgg cctagcctct agtctcctga gcccacataa
     3121 ccctcaggaa actgggaccc aaagaacttc accgcacact tactgagtgc agagagcagc
     3181 ttccctggct ttgctcactt gcagacacag agcgcagggc agatggaggc tcgaaagcac
     3241 tttccttgtg catttggaga gtggcattgc cttttagata ggatctagga gtgattttat
     3301 tgtgtgggag aatggaaggg tccagtggcc ctggctttgt catcagtgac tgccatagca
     3361 acagcagctc tgtccctcgt cttttgagcc cacctttgaa gaggagacac agtactcacc
     3421 ttaattgcac tggtagcagc tcatttcctg tttatcattt tcatcattaa ttggaagctg
     3481 ccttgggatt taagcaccag gcattgcaca tctggatgga tgaaggaaac tataaaaatg
     3541 gagtgggctg cccaggctcc tccagagagt ggtaaagtat tcatgccttc tcagaagcct
     3601 ggagtccaga aaaatttact ggtagagtca gtcagtctag agcttatttt caggaagttc
     3661 ggtcacttca tgcagctgag tgcttcatga ggaagcttag attgctttca ctgtggaggt
     3721 tgtttgaaac atcatcagtg aggtgcagag aaagggcagg tccctcacca caatagccaa
     3781 actttgaggc tacagttagt acaagatggg gtttcccagt tctgggggat tcctccaaga
     3841 caaaaggtgt ctgagcaaat tacaccacca tcaagtagta gatagagctg tgggggaaaa
     3901 tcatgggggg aggtaatact tccttcagtg ccttgccttg gtatctgaga gggcctcagt
     3961 gacactgagg tcttctgtcc ctgtgaaggc ttctttgtac ctgtagtgtc atggccctta
     4021 ggggtctgat tgggataatc aaccctgagc actccctttc tatgtctagt caatattgtc
     4081 ccctctggtg tgttttggag ccatgtcaga ccaatcagtg tcatcttttg tacttgagta
     4141 gcccaaagcc agcaccaaat ccttggggtc acaaaagctt gacaggtaga acctgccaag
     4201 taagagggaa ttagctgcaa gctcactctg tgttcttgtt gagaggagca agccaccgtt
     4261 cccaaagaca ataaatgccc tagaagagcc aaccttccag gcagacattt ggtagtttcc
     4321 cttaatgtga gccttgtgct tccaccaagt ctaatggatt gcttgtgttt gaggccactg
     4381 cagctccttt gataagtacc acatcctctg tgctacatac agctaaatta atagtgtttc
     4441 atttaaccaa accaaaaaaa ggtttgctat ctcttttaat attgcaggag gacctaccta
     4501 tgcaggagaa attttgccat ttccttaagc tagtggtaca aaggctggtg ctgggaagaa
     4561 gcccatctct gaggatgggc aactctgtcc aatgggatca ctgatgtccc ttgttttctc
     4621 agttctgaga agctcagtac agtagtacag atgtcatcac tgaagctttt ttccagaata
     4681 aggaaggccc aatccaggtg ttacaagttc tagggtctgg cagtcagccc tgtcagagtt
     4741 ttggttcagt ttggtcagta caactttggt tttgtcagga ctggctggcc tttcaagttc
     4801 ctgtgtcctc atgcttacca tctcagcagg cctgagaggc agggtctgag gtgctcggtg
     4861 tgagcgctgc ttctgctctg gagctgaggt gtgggcattg ctgactcggg ggtgacagcg
     4921 agtcttgaag caggcaggag tcctggggag tcatgctcaa gtacagcatg gcggtcgttt
     4981 tctctagtct gttcctttgg gaagtgtagg tttgctatta atctagctga gaatctaagt
     5041 tctgtgcctt agagacaatt tgcactttcc agaattgtgc ctacgacagc cataagattt
     5101 tttccctacc aaacagctat gcaaacaaaa accagttcaa aagtcacaac catccactgg
     5161 gtttagtaaa agtgaagaca gcagaaatgt ctcaaatggt ctctctagct aaatcttcgt
     5221 aaattgtcct gctcgccctc catgcagtgc tgggtgattg aagccttcca gtagtgctgc
     5281 tttgagttac agtatagcct gcgtctatcc tctgtgccaa cactggctca agagccagct
     5341 tcttgttagc ctgatgctgg aaaggatgag gatgaagtgg tgtggcgttt ctgtttaaat
     5401 aaacgtttaa actctca
//
