LOCUS       NM_001105804            2631 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus NIMA-related kinase 8 (Nek8), mRNA.
ACCESSION   NM_001105804 XM_001080757 XM_006246933 XM_220639
VERSION     NM_001105804.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Nakajima,Y., Kiyonari,H., Mukumoto,Y. and Yokoyama,T.
  TITLE     The Inv compartment of renal cilia is an intraciliary
            signal-activating center to phosphorylate ANKS6
  JOURNAL   Kidney Int 93 (5), 1108-1117 (2018)
   PUBMED   29395339
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   3  (bases 1 to 2631)
  AUTHORS   Frank,V., Habbig,S., Bartram,M.P., Eisenberger,T.,
            Veenstra-Knol,H.E., Decker,C., Boorsma,R.A., Gobel,H., Nurnberg,G.,
            Griessmann,A., Franke,M., Borgal,L., Kohli,P., Volker,L.A.,
            Dotsch,J., Nurnberg,P., Benzing,T., Bolz,H.J., Johnson,C.,
            Gerkes,E.H., Schermer,B. and Bergmann,C.
  TITLE     Mutations in NEK8 link multiple organ dysplasia with altered Hippo
            signalling and increased c-MYC expression
  JOURNAL   Hum Mol Genet 22 (11), 2177-2185 (2013)
   PUBMED   23418306
REFERENCE   4  (bases 1 to 2631)
  AUTHORS   McCooke,J.K., Appels,R., Barrero,R.A., Ding,A., Ozimek-Kulik,J.E.,
            Bellgard,M.I., Morahan,G. and Phillips,J.K.
  TITLE     A novel mutation causing nephronophthisis in the Lewis polycystic
            kidney rat localises to a conserved RCC1 domain in Nek8
  JOURNAL   BMC Genomics 13, 393 (2012)
   PUBMED   22899815
  REMARK    GeneRIF: A novel Nek8 mutation R650C is that occurs within the
            regulator of chromosome condensation 1 (RCC1)-like region of the
            protein.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2631)
  AUTHORS   Shiba,D., Manning,D.K., Koga,H., Beier,D.R. and Yokoyama,T.
  TITLE     Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal
            segment of primary cilia
  JOURNAL   Cytoskeleton (Hoboken) 67 (2), 112-119 (2010)
   PUBMED   20169535
REFERENCE   6  (bases 1 to 2631)
  AUTHORS   Otto,E.A., Trapp,M.L., Schultheiss,U.T., Helou,J., Quarmby,L.M. and
            Hildebrandt,F.
  TITLE     NEK8 mutations affect ciliary and centrosomal localization and may
            cause nephronophthisis
  JOURNAL   J Am Soc Nephrol 19 (3), 587-592 (2008)
   PUBMED   18199800
REFERENCE   7  (bases 1 to 2631)
  AUTHORS   Mahjoub,M.R., Trapp,M.L. and Quarmby,L.M.
  TITLE     NIMA-related kinases defective in murine models of polycystic
            kidney diseases localize to primary cilia and centrosomes
  JOURNAL   J Am Soc Nephrol 16 (12), 3485-3489 (2005)
   PUBMED   16267153
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On or before Dec 5, 2020 this sequence version replaced
            XM_006246933.2, NM_001105804.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360194.157952.1,
                                           SRR26643286.31230.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               JAXUCZ010000010.1  63570382-63570488   c
            108-313             JAXUCZ010000010.1  63566305-63566510   c
            314-546             JAXUCZ010000010.1  63565846-63566078   c
            547-678             JAXUCZ010000010.1  63565512-63565643   c
            679-887             JAXUCZ010000010.1  63564737-63564945   c
            888-967             JAXUCZ010000010.1  63564426-63564505   c
            968-1149            JAXUCZ010000010.1  63564162-63564343   c
            1150-1300           JAXUCZ010000010.1  63563923-63564073   c
            1301-1377           JAXUCZ010000010.1  63562988-63563064   c
            1378-1495           JAXUCZ010000010.1  63562509-63562626   c
            1496-1646           JAXUCZ010000010.1  63561145-63561295   c
            1647-1810           JAXUCZ010000010.1  63560783-63560946   c
            1811-1969           JAXUCZ010000010.1  63560522-63560680   c
            1970-2128           JAXUCZ010000010.1  63560204-63560362   c
            2129-2631           JAXUCZ010000010.1  63559315-63559817   c
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q25"
     gene            1..2631
                     /gene="Nek8"
                     /note="NIMA-related kinase 8"
                     /db_xref="GeneID:287473"
                     /db_xref="RGD:1306897"
     exon            1..107
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..2157
                     /gene="Nek8"
                     /EC_number="2.7.11.1"
                     /note="NIMA (never in mitosis gene a)-related expressed
                     kinase 8; NIMA (never in mitosis gene a)- related kinase
                     8; nimA-related protein kinase 8; never in mitosis
                     A-related kinase 8"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek8"
                     /protein_id="NP_001099274.1"
                     /db_xref="GeneID:287473"
                     /db_xref="RGD:1306897"
                     /translation="MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEE
                     RQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEE
                     ETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKA
                     YTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSG
                     TFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRR
                     AEKCLTPGTPMAPGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSIPLRLP
                     MPNTEVVQVAAGRTQKAGVTRSGRLILWEAPPLGTGGGTLLPGAVELPQPQFVSRFLE
                     GQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYE
                     MVQVACGASHVLALSADGELFAWGRGDGGRLGLGTRESHNCPQQVPMVPGQEAQRVVC
                     GIDCSMILTSPGRVLACGSNRFNKLGLDCLSLEEEPVPHQQVEEALSFTPLGSAPLDR
                     ETLLCVDLGTAHSAAVTASGACYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMV
                     ACGDAFTVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHG
                     NTLLAVRSVTDEPVPP"
     exon            108..313
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            314..546
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            547..678
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            679..887
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            888..967
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1149
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1300
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1377
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1495
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1646
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1810
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1969
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2128
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2631
                     /gene="Nek8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggatgggagg gtccgcgcac gcgccactcg aggagtccag aagcgtaact gagaaatgag
       61 atggagaagt acgagcggat ccgagtggtg ggaagaggtg ccttcgggat tgtgcacctg
      121 tgccttcgaa aggctgacca gaagctggtg atcatcaagc agatcccagt ggaacagatg
      181 accaaagagg agaggcaggc ggcacagaat gagtgccagg tgctcaaact gctcaaccac
      241 cccaacgtca ttgagtacta cgagaacttc ctggaggaca aggccctcat gatcgccatg
      301 gaatatgcac caggtggcac cctggctgag ttcatccaga agcgctgcaa ctccctgctg
      361 gaggaggaga cgatccttca cttctttgtg cagatcctgc ttgcgctgca tcatgtgcac
      421 acacacctca tcctgcatcg ggacctcaag acccagaaca tccttcttga caagcaccgc
      481 atggtcgtca agatcgggga ctttggcatc tctaagatcc ttagcagcaa gagcaaggcc
      541 tacacggtgg tgggtactcc atgctacatc tcccctgagc tatgtgaggg caagccctac
      601 aaccagaaga gtgacatctg ggctctgggc tgtgtcttat atgagcttgc cagtctcaag
      661 agggcctttg aggcagcgaa cttgcccgcc ctggtgctga agatcatgag cggcaccttt
      721 gcacccatct ctgaccgtta tagtcctgag ctacgccagc ttgtcctgag tctgctcagc
      781 ctggaacctg cacagcggcc acccctcagc cacatcatgg ctcagcccct ctgcatccgg
      841 gccctactca acatccacac cgatgtgggc agcgtccgca tgcggagggc agagaagtgc
      901 ctgaccccag gaacacctat ggccccgggc agcacaggga gcagggccac cagtgcccga
      961 tgcaggggtg tcccccgggg acctgtgaga ccggccatcc cgccgcctct gtcttccgtc
     1021 tacgcttggg gtggtggcct tagcattccc ctgaggttgc cgatgcccaa cacggaggtg
     1081 gtccaggtgg ccgcagggcg cacacagaag gctggcgtta cacgctcagg acgcctcatt
     1141 ctgtgggagg ccccacccct gggaactgga ggcggcacac tccttccggg ggcagtggag
     1201 ctgcctcagc cccagtttgt ctctcgtttc ctggagggcc agtcaggcgt gaccatcaag
     1261 catgtggcct gcggggacct gttcactgct tgcttgacgg acagaggcat catcatgact
     1321 tttggcagcg gcagcaacgg gtgtttaggt cacggcagcc tgactgacat cagccagccc
     1381 accattgtag aggccctgct ggggtatgag atggtgcagg tggcctgtgg agcctctcat
     1441 gtgctggccc tgtccgcaga tggagagtta tttgcctggg gccgaggaga tggcggtagg
     1501 ctgggactag gcaccaggga gtcccataac tgccctcagc aggtgcctat ggtcccagga
     1561 caggaagctc agagagttgt ctgtggtatt gactgttcca tgatcctcac ctcaccaggc
     1621 agggtcctgg cctgtggaag taacaggttc aacaagctag gcttggactg cctgtccctg
     1681 gaggaggagc ctgttcccca ccagcaagtg gaggaggccc tgagcttcac gccgctgggt
     1741 tctgcacctc tggaccggga gaccctgctg tgcgtggacc ttggcactgc tcattcagct
     1801 gctgtgactg cttccggggc ctgctatact tttggcagca atcagcatgg gcaattgggc
     1861 accagttccc gccgggtcag cagggcaccc tgtcgggtcc aaggcctaga gggcatcaag
     1921 atggtgatgg tggcctgtgg ggatgccttc actgtggcca ttggggcaga gggggaagtg
     1981 tattcttggg gcaaaggagc ccggggtcgc ctgggaagga gggatgagga tgccggactc
     2041 cctagaccag tgcagctgga tgagactcat ccttacacgg tgacatcagt gtcctgctgc
     2101 catgggaaca ctcttttggc tgttcgatca gtcacagatg aaccagttcc cccctgaggc
     2161 aacaagatta tccaagagac cacctctgag tgttccgaaa aatgcaggtg cttgaggaat
     2221 gactgttttc agcgtcgccc tggattgtgt catcaatggg gtagaaaggc cagtgacatc
     2281 tactctgttc ccccaagtcc ctgtccccga actctttatc ttactatctc caggatccag
     2341 tctggtagga atcaacagaa ggatggcctt caaagacttg actaaaaaaa gcaccttccc
     2401 tgggctggag agatagctca gcagttaaga gcactgactg ctcttcctga ggtcctgagt
     2461 tcaaatccca gtaaccacat ggtggctcac aaccatctgt aatgagatcc cgtgccctct
     2521 tctggtgtgt ctgaagacag ctacagtata tgtatataat acacacacac acacacacac
     2581 acacacacac acacacacac acatataata aatcttaaaa aaaaaaaaaa a
//
