LOCUS       NM_053691               3122 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus NIMA-related kinase 2 (Nek2), mRNA.
ACCESSION   NM_053691 XM_001055166 XM_001069107
VERSION     NM_053691.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Chen,C., Tian,F., Lu,L., Wang,Y., Xiao,Z., Yu,C. and Yu,X.
  TITLE     Characterization of Cep85 - a new antagonist of Nek2A that is
            involved in the regulation of centrosome disjunction
  JOURNAL   J Cell Sci 128 (17), 3290-3303 (2015)
   PUBMED   26220856
  REMARK    Erratum:[J Cell Sci. 2015 Oct 15;128(20):3837. PMID: 26471995]
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Fang,G., Zhang,D., Yin,H., Zheng,L., Bi,X. and Yuan,L.
  TITLE     Centlein mediates an interaction between C-Nap1 and Cep68 to
            maintain centrosome cohesion
  JOURNAL   J Cell Sci 127 (Pt 8), 1631-1639 (2014)
   PUBMED   24554434
REFERENCE   3  (bases 1 to 3122)
  AUTHORS   Cerone,M.A., Burgess,D.J., Naceur-Lombardelli,C., Lord,C.J. and
            Ashworth,A.
  TITLE     High-throughput RNAi screening reveals novel regulators of
            telomerase
  JOURNAL   Cancer Res 71 (9), 3328-3340 (2011)
   PUBMED   21531765
REFERENCE   4  (bases 1 to 3122)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   5  (bases 1 to 3122)
  AUTHORS   Mardin,B.R., Lange,C., Baxter,J.E., Hardy,T., Scholz,S.R., Fry,A.M.
            and Schiebel,E.
  TITLE     Components of the Hippo pathway cooperate with Nek2 kinase to
            regulate centrosome disjunction
  JOURNAL   Nat Cell Biol 12 (12), 1166-1176 (2010)
   PUBMED   21076410
REFERENCE   6  (bases 1 to 3122)
  AUTHORS   Lou,Y., Yao,J., Zereshki,A., Dou,Z., Ahmed,K., Wang,H., Hu,J.,
            Wang,Y. and Yao,X.
  TITLE     NEK2A interacts with MAD1 and possibly functions as a novel
            integrator of the spindle checkpoint signaling
  JOURNAL   J Biol Chem 279 (19), 20049-20057 (2004)
   PUBMED   14978040
REFERENCE   7  (bases 1 to 3122)
  AUTHORS   Di Agostino,S., Fedele,M., Chieffi,P., Fusco,A., Rossi,P.,
            Geremia,R. and Sette,C.
  TITLE     Phosphorylation of high-mobility group protein A2 by Nek2 kinase
            during the first meiotic division in mouse spermatocytes
  JOURNAL   Mol Biol Cell 15 (3), 1224-1232 (2004)
   PUBMED   14668482
REFERENCE   8  (bases 1 to 3122)
  AUTHORS   Yoo,J.C., Chang,J.R., Kim,S.H., Jang,S.K., Wolgemuth,D.J., Kim,K.
            and Rhee,K.
  TITLE     NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting
            specific roles of Nek2 in Golgi
  JOURNAL   Exp Cell Res 292 (2), 393-402 (2004)
   PUBMED   14697346
REFERENCE   9  (bases 1 to 3122)
  AUTHORS   Ha Kim,Y., Yeol Choi,J., Jeong,Y., Wolgemuth,D.J. and Rhee,K.
  TITLE     Nek2 localizes to multiple sites in mitotic cells, suggesting its
            involvement in multiple cellular functions during the cell cycle
  JOURNAL   Biochem Biophys Res Commun 290 (2), 730-736 (2002)
   PUBMED   11785960
REFERENCE   10 (bases 1 to 3122)
  AUTHORS   Helps,N.R., Luo,X., Barker,H.M. and Cohen,P.T.
  TITLE     NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase
            localized to centrosomes, is complexed to protein phosphatase 1
  JOURNAL   Biochem J 349 (Pt 2), 509-518 (2000)
   PUBMED   10880350
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            EV771094.1, AF352021.1 and JAXUCZ010000013.1.
            
            On Jul 6, 2012 this sequence version replaced NM_053691.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR21887620.523292.1,
                                           SRR26643297.42537.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               EV771094.1         1-134
            135-725             AF352021.1         1-591
            726-732             AF352021.1         593-599
            733-739             AF352021.1         601-607
            740-1310            AF352021.1         609-1179
            1311-2083           JAXUCZ010000013.1  105948243-105949015
            2084-3122           JAXUCZ010000013.1  105949016-105950054
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q27"
     gene            1..3122
                     /gene="Nek2"
                     /note="NIMA-related kinase 2"
                     /db_xref="GeneID:114482"
                     /db_xref="RGD:619792"
     exon            1..218
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..1454
                     /gene="Nek2"
                     /EC_number="2.7.11.1"
                     /note="NIMA (never in mitosis gene a)-related kinase 2;
                     NIMA (never in mitosis gene a)-related expressed kinase 2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek2"
                     /protein_id="NP_446143.1"
                     /db_xref="GeneID:114482"
                     /db_xref="RGD:619792"
                     /translation="MPSRVEDYEVLHSIGTGSYGRCQKIRRKSDGKILVWKELDYGSM
                     TEVEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGT
                     KDRQYLEEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDSKHNVKLGDF
                     GLARILNHDTSFAKTFVGTPYYMSPEQISRLSYNEKSDIWSLGCLLYELCALMPPFTA
                     FNQKELAGKIREGRFRRIPYRYSDGLNDLITRMLNLKDYHRPSVEEILESPLIADLVA
                     EEQRRNLERRGRRSGEPSKLQDSSPVLSELKLKERQLQDRERALRAREDSLEQKEREL
                     CIRERLAEDKLARAESLLKNYSLLKEHRLLCLAGGSELDLPSSALKKKVHFHGESKEN
                     TARSENSESQLAKSKCRDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR"
     exon            219..436
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     exon            437..677
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     exon            678..760
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     exon            761..887
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1107
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1233
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..3122
                     /gene="Nek2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccggttg ctccgctccg acaagcagac tagggtcccc ggggtcggcg ctgttggcgt
       61 gcggcgggcg agcctggact ccgagcggcg cggtagtgtg gttgccgggg tggccgctcg
      121 tcatgccgtc ccgggtggag gactacgagg tgctgcacag catcggcacc ggctcctacg
      181 gccgctgtca gaagattcgg aggaagagcg acggcaagat cctggtgtgg aaagaacttg
      241 actatggctc catgacagag gtggagaaac aaatgcttgt gtctgaagtg aacttgcttc
      301 gtgagctgaa acatccaaac atcgtccgtt actacgatcg tattatcgac agaaccaaca
      361 caaccctata cattgtcatg gagtactgtg agggcgggga cctggctagt gtcattacaa
      421 aggggaccaa ggatagacaa tacttagagg aagagtttgt ccttcgagtg atgactcagt
      481 tgacactggc cctgaaagag tgtcacagaa ggagcgatgg tggccacact gtacttcacc
      541 gggacctaaa gccagccaat gtcttcctgg acagcaagca caatgtcaag cttggggact
      601 ttgggctagc tagaatattg aatcacgaca caagttttgc aaagacgttt gttggcacac
      661 cctattacat gtctcctgag caaataagcc gcttgtctta caatgagaag tcggacatct
      721 ggtcgctggg ctgcctgctg tatgagctgt gtgcactaat gcctccattt acagctttca
      781 accagaaaga gttagcgggg aaaatcaggg aagggaggtt ccggcgcatc ccctaccgct
      841 actctgatgg cttgaatgac ctcatcactc ggatgctgaa tttaaaggac taccatcgac
      901 cttcagtgga ggaaattctg gagagccctt tgatagcaga cttggttgca gaagagcaaa
      961 ggagaaatct ggagaggagg ggacggcgct caggcgagcc ttcgaagctg caggactcca
     1021 gccctgtgct gagtgagctc aagttgaagg aaaggcagct gcaggatcga gagcgagcac
     1081 tgagagctcg ggaggacagc ctggagcaga aagaacgtga actttgtatt cgagagagac
     1141 ttgccgagga caaactggcc agagccgaga gtctgctgaa gaactacagc ctgctgaagg
     1201 agcacaggct cctgtgtctg gctggcggct cagaacttga tctcccatct tcagcactga
     1261 agaagaaggt tcatttccat ggggagagca aagagaacac cgcaaggagt gagaattccg
     1321 agagccagct cgccaagtcc aagtgcaggg acctgaagaa gaggcttcat gctgcccagc
     1381 tgcgtgctca agccctgtct gatattgaga agaactacca gctaaagagc aggcagatcc
     1441 tgggcatgcg ttaggccagc aaggcatgga gccgggtcag cactggtgct gacaacgcac
     1501 gagagattgg actcagctgc tgtagtgtgt gtgtgtggtg agcatggccc tgggattgtt
     1561 tgtggtctcc gagatggaca tgtcctcttc ctaatccccc tgtgtagaaa gcatgctgtc
     1621 tttgctgagt gcttgggctt ttgatcctgt gtgcgattac tacttgaaat atgagatggg
     1681 acactataga taccatagat cttaggagaa acaatgttag ggaaagaaca tttaacctgg
     1741 aggagttgga gaggtttgct caactgagag atgcggcctt catggcagtt tgcaggtgag
     1801 gcttcagcag gtatgtgatc ttgctgctaa cggaataggg cagggcccac agggttccgt
     1861 ccatgtcaga ggtgcggtga gcttatcagt gtgtgatgct acagggagag acccagcctc
     1921 tggcctgagc ctgaggagaa gccactcacc agcattccag ttccaagatg ctcctggtgc
     1981 tagttaaaat gcagattaga cttgggaggg aagaacaagg gctgaagcct ttactcacct
     2041 tggggaaact cgttctagca cagcacccag tgagactgac aaggcttgca ctagagacag
     2101 gctgctggga gccctgagag cttcctcccc actgtgtgca tggcctgtcc ccttctgtct
     2161 gtatctgacc tcaatgtcag ttgataacat tattaagtgc tgtctgtgcc ctcaagtatg
     2221 agctgaatgg tcggaaatgc cacagagtct atatatttta aatcacacat gtggtcccca
     2281 aacattgaat gttggagaac tgtcttcaga aagttggtct atttaaggct actggggaaa
     2341 agggcgggtc tcctttgcta tctgtccaca gcccctttgc ttcagcaaag aacagtgtgc
     2401 atgcatgcag tctgtgtttg agagccatgc gagccttcac ctctgtgagg gccagatggc
     2461 tgtttgtcta cagtttcctg aaggcctcag cagaaaggga ttcctttttc ctagtaagtg
     2521 ctgctggctg agaggaggtc agaagtattt taatgccatc tgtttcatgg ctaaacacgc
     2581 agactccttg cattttaaaa ttctgtgtcc tttacttttt ggagctatac tagcttgctt
     2641 gggtgtgtaa cttgccagag ttgacttttc tagtgcttta tgtaaactct gctgttggca
     2701 cttgcagctg aagcagctga aagaggggct gctcactgtt gcagcactgg gatccctggc
     2761 agctgcagcg gcaatgctta aggagctttt gcaactttga cttcagtagc agcttgggat
     2821 gataaaatat ggtgagtgtc ttgtctgctg tggagggcct gacaatctgc caggtgggaa
     2881 gtcttgttag aaaccagaaa ctcagtgtgg tggttatccc accttttctt tctttctcct
     2941 ttggtctata atgatattga catagctatt cagagtaaaa tctatgtata caaaaaaatt
     3001 ctaagtatga ggttttaaat gttcttgaac ttcagaaaaa cttgattgga tacaattgtt
     3061 tataattaac aagtcatctt atttataaat tttccaatat aatataaatc tattttgtgg
     3121 aa
//
