LOCUS       NM_017029               3067 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus neurofilament medium chain (Nefm), mRNA.
ACCESSION   NM_017029
VERSION     NM_017029.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Mages,B., Aleithe,S., Altmann,S., Blietz,A., Nitzsche,B.,
            Barthel,H., Horn,A.K.E., Hobusch,C., Hartig,W., Krueger,M. and
            Michalski,D.
  TITLE     Impaired Neurofilament Integrity and Neuronal Morphology in
            Different Models of Focal Cerebral Ischemia and Human Stroke Tissue
  JOURNAL   Front Cell Neurosci 12, 161 (2018)
   PUBMED   29967576
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Uzasci,L., Auh,S., Cotter,R.J. and Nath,A.
  TITLE     Mass spectrometric phosphoproteome analysis of HIV-infected brain
            reveals novel phosphorylation sites and differential
            phosphorylation patterns
  JOURNAL   Proteomics Clin Appl 10 (2), 126-135 (2016)
   PUBMED   26033855
REFERENCE   3  (bases 1 to 3067)
  AUTHORS   Yuan,A., Sershen,H., Veeranna, Basavarajappa,B.S., Kumar,A.,
            Hashim,A., Berg,M., Lee,J.H., Sato,Y., Rao,M.V., Mohan,P.S.,
            Dyakin,V., Julien,J.P., Lee,V.M. and Nixon,R.A.
  TITLE     Neurofilament subunits are integral components of synapses and
            modulate neurotransmission and behavior in vivo
  JOURNAL   Mol Psychiatry 20 (8), 986-994 (2015)
   PUBMED   25869803
REFERENCE   4  (bases 1 to 3067)
  AUTHORS   Stegeman,S., Jolly,L.A., Premarathne,S., Gecz,J., Richards,L.J.,
            Mackay-Sim,A. and Wood,S.A.
  TITLE     Loss of Usp9x disrupts cortical architecture, hippocampal
            development and TGFbeta-mediated axonogenesis
  JOURNAL   PLoS One 8 (7), e68287 (2013)
   PUBMED   23861879
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3067)
  AUTHORS   Richard,A.F., Demignon,J., Sakakibara,I., Pujol,J., Favier,M.,
            Strochlic,L., Le Grand,F., Sgarioto,N., Guernec,A., Schmitt,A.,
            Cagnard,N., Huang,R., Legay,C., Guillet-Deniau,I. and Maire,P.
  TITLE     Genesis of muscle fiber-type diversity during mouse embryogenesis
            relies on Six1 and Six4 gene expression
  JOURNAL   Dev Biol 359 (2), 303-320 (2011)
   PUBMED   21884692
REFERENCE   6  (bases 1 to 3067)
  AUTHORS   Kelly,B.M., Gillespie,C.S., Sherman,D.L. and Brophy,P.J.
  TITLE     Schwann cells of the myelin-forming phenotype express neurofilament
            protein NF-M
  JOURNAL   J Cell Biol 118 (2), 397-410 (1992)
   PUBMED   1321159
REFERENCE   7  (bases 1 to 3067)
  AUTHORS   Xu,Z.S., Liu,W.S. and Willard,M.B.
  TITLE     Identification of six phosphorylation sites in the COOH-terminal
            tail region of the rat neurofilament protein M
  JOURNAL   J Biol Chem 267 (7), 4467-4471 (1992)
   PUBMED   1537832
REFERENCE   8  (bases 1 to 3067)
  AUTHORS   Paz,M.M., Valente,G.B., Tasca,C.I., de Mattos,A.G. and Pureur,R.P.
  TITLE     Malnutrition induces an increase in intermediate filament protein
            content of rat cerebral cortex
  JOURNAL   J Nutr 121 (9), 1349-1354 (1991)
   PUBMED   1908892
REFERENCE   9  (bases 1 to 3067)
  AUTHORS   Schlaepfer,W.W. and Bruce,J.
  TITLE     Simultaneous up-regulation of neurofilament proteins during the
            postnatal development of the rat nervous system
  JOURNAL   J Neurosci Res 25 (1), 39-49 (1990)
   PUBMED   2108255
REFERENCE   10 (bases 1 to 3067)
  AUTHORS   Napolitano,E.W., Chin,S.S., Colman,D.R. and Liem,R.K.
  TITLE     Complete amino acid sequence and in vitro expression of rat NF-M,
            the middle molecular weight neurofilament protein
  JOURNAL   J Neurosci 7 (8), 2590-2599 (1987)
   PUBMED   2441012
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from Z12152.1.
            
            [WARNING] On Oct 7, 2024 this sequence was replaced by NM_017029.3.
            
            On Aug 31, 2012 this sequence version replaced NM_017029.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z12152.1, M18628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3067              Z12152.1           4-3070
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..3067
                     /gene="Nefm"
                     /gene_synonym="Nef3; Nfm"
                     /note="neurofilament medium chain"
                     /db_xref="GeneID:24588"
                     /db_xref="RGD:3160"
     exon            1..1109
                     /gene="Nefm"
                     /gene_synonym="Nef3; Nfm"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..2573
                     /gene="Nefm"
                     /gene_synonym="Nef3; Nfm"
                     /note="Neurofilament protein, middle polypeptide;
                     neurofilament 3, medium; NF-M; 160 kDa neurofilament
                     protein; neurofilament triplet M protein; neurofilament,
                     medium polypeptide"
                     /codon_start=1
                     /product="neurofilament medium polypeptide"
                     /protein_id="NP_058725.1"
                     /db_xref="GeneID:24588"
                     /db_xref="RGD:3160"
                     /translation="MSYTLDSLGNPSAYRRVTETPSSFSRVSGSPSSGFRSQSWSRGS
                     PSTVSSSYKRSALAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQ
                     LQGLNDRFAGYIEKVHYLEQQNKEIEAEIHALRQKQASHAQLGDAYDQEIRELRATLE
                     MVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSMVKVEL
                     DKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLE
                     CHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRG
                     TKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMAL
                     DIEIAAYRKLLEGEETRFSTFSGSITGPLYTHRQPSVTISSKIQKTKVEAPKLKVQHK
                     FVEEIIEETKVEDEKSEMEDALTVIAEELAASAKEEKEEAEEKEEEPEVEKSPVKSPE
                     AKEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEEEGETEAE
                     GEGEEAEAKEEKKTEGKVEEMAIKEEIKVEKPEKAKSPVPKSPVEEVKPKPEAKAGKD
                     EQKEEEKVEEKKEVAKESPKEEKVEKKEEKPKDVPDKKKAESPVKEKAVEEMITITKS
                     VKVSLEKDTKEEKPQQQEKVKEKAEEEGGSEEEVGDKSPQESKKEDIAINGEVEGKEE
                     EEQETQEKGSGQEEEKGVVTNGLDVSPAEEKKGEDRSDDKVVVTKKVEKITSEGGDGA
                     TKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQGD"
     exon            1110..1234
                     /gene="Nefm"
                     /gene_synonym="Nef3; Nfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..3064
                     /gene="Nefm"
                     /gene_synonym="Nef3; Nfm"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acgcgtgggc agccacacac cccaaggcct ccaagatgag ctacacgctg gactcgctgg
       61 gcaacccgtc cgcctaccgg cgcgtcaccg agaccccgtc cagcttcagt cgtgtgagcg
      121 gttccccgtc cagcggcttc cgctcgcagt cctggtcccg cggctcgccc agcaccgtgt
      181 cctcctccta caagcgcagc gcgctcgccc cgcgcctcgc ctacagctcg gctatgctca
      241 gctcggccga gagcagcctc gacttcagcc agtcctcttc gctgcttaac ggcggctccg
      301 gcggcgacta caagctgtcc cgctcaaacg agaaagagca gctgcagggg ctgaacgacc
      361 gtttcgccgg ctacatcgag aaagtgcact acttggaaca acagaacaag gagatcgagg
      421 cagagatcca cgcgctgcgg cagaagcagg cctcgcacgc ccagctgggt gacgcttacg
      481 accaggagat ccgagagctg cgcgccaccc tggagatggt gaatcacgag aaggctcaag
      541 tgcagctgga ctctgatcac ttggaggaag acatccaccg gctcaaggag cgcttcgagg
      601 aggaggcgcg gctgcgggac gacaccgagg ctgccatccg ggcgctgcgc aaagacatag
      661 aggagtcgtc gatggttaag gtggagctgg acaagaaggt gcagtcgctg caggatgagg
      721 tggccttcct gcggagcaat cacgaagagg aggtggccga cctgctggcc cagatccagg
      781 cgtcgcacat caccgtagag cgcaaagact acctgaagac agacatctcc acggcgctga
      841 aagagatccg ctcccagctc gagtgtcact ccgaccagaa catgcaccag gccgaagagt
      901 ggttcaaatg ccgctacgcc aagctcaccg aggcggccga gcagaacaag gaggccatcc
      961 gctccgctaa agaagagatc gccgagtacc ggcgccagct gcagtccaag agcattgagc
     1021 tcgagtcggt gcgaggcact aaggagtccc tggaacggca gctcagcgac atcgaggagc
     1081 gccacaacca cgacctcagc agctaccagg acaccatcca gcagctggaa aatgagcttc
     1141 ggggaacaaa gtgggaaatg gctcgtcatt tgcgagaata ccaggatctc cttaacgtca
     1201 agatggctct ggacatcgag atcgccgcat ataggaaact actggagggt gaagagacca
     1261 gatttagcac attttcagga agcatcactg ggcctctgta cacacaccga cagccctcag
     1321 tcacaatatc cagtaagatt cagaagacca aagtcgaggc ccccaagctc aaggtccaac
     1381 acaaatttgt ggaggagatc attgaggaga ctaaagtgga agatgagaag tcagaaatgg
     1441 aagacgccct cacagtcatt gcagaggaat tggcagcctc tgccaaagag gagaaagaag
     1501 aggcagaaga aaaggaagag gaaccggaag ttgaaaagtc tcccgtgaag tctcctgagg
     1561 ctaaggaaga ggaggaaggg gaaaaggagg aagaagagga aggccaagag gaagaagagg
     1621 aggaagatga aggtgtcaag tcagaccagg cagaagaggg aggatctgag aaggaaggct
     1681 cgagtgaaaa ggatgaaggt gagcaagaag aagaagggga aactgaggca gaaggtgaag
     1741 gagaggaagc agaagctaag gaggaaaaga aaacagaggg aaaggtcgag gaaatggcta
     1801 tcaaggagga aatcaaggtc gagaagcccg agaaagccaa gtcccctgtg ccaaaatcac
     1861 ccgtggaaga agtaaagcca aaaccagaag ccaaagccgg aaaggatgag cagaaggagg
     1921 aagagaaagt tgaggagaag aaggaggtag ccaaggaatc acccaaggaa gagaaggtgg
     1981 agaaaaagga ggagaagcca aaagatgtcc cagataaaaa gaaggctgag tccccagtga
     2041 aagaaaaggc cgtagaggaa atgatcacca ttactaagtc ggtaaaggtg agcctggaga
     2101 aagacaccaa agaggagaag cctcagcagc aggagaaggt gaaggagaag gcagaggagg
     2161 aggggggtag tgaggaggaa gtgggtgaca aaagcccgca agaatccaag aaggaagaca
     2221 tagctatcaa tggggaggtg gaaggaaaag aggaggagga gcaggaaact caggagaagg
     2281 gcagtgggca agaggaggag aaaggggtgg tcactaatgg cttagatgtg agccctgcgg
     2341 aggaaaagaa aggggaggat agaagtgatg acaaagtggt ggtgaccaag aaggtagaaa
     2401 aaatcaccag cgagggaggc gatggtgcta ccaaatacat caccaaatct gttactgtca
     2461 ctcaaaaggt tgaagagcat gaggagacct ttgaggagaa gctggtgtca actaaaaagg
     2521 tagaaaaggt cacttcacat gccatagtca aggaagtcac ccagggtgac taagatcgga
     2581 gtccattgca aaaggttaag ccatacaaca atttcaaaat gcatgtgatt gacagcttca
     2641 aaacagaatg ggttctccca tgagggctcc agacattgta ttttcctttg tgcaatatga
     2701 gggaactgca tgcaagctca gggtgccccc ctcctcagtc cttgggggaa ttcaaatgca
     2761 tgttcatgta tgtacctagg gaatttgcca gtttcctaag ctgttgaaag aggggcactc
     2821 ggggggatgt cttgagatgt attacgcaaa gtaccaactg agccaaaaat aataagtgaa
     2881 acagaactct cttagcctta agaaagctat atatgaatac ttatgtttac ctcactggtg
     2941 catttaaaat ggacttcagt tcatgggaga accttgctga cctgcacagt tcgcaacctt
     3001 atgttgattg atgttaaatg tcacagcagt tcttgctcaa taaaggtcat actggaacca
     3061 taaaaaa
//
