LOCUS       NP_058725                845 aa            linear   ROD 03-APR-2024
DEFINITION  neurofilament medium polypeptide [Rattus norvegicus].
ACCESSION   NP_058725
VERSION     NP_058725.1
DBSOURCE    REFSEQ: accession NM_017029.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 845)
  AUTHORS   Mages,B., Aleithe,S., Altmann,S., Blietz,A., Nitzsche,B.,
            Barthel,H., Horn,A.K.E., Hobusch,C., Hartig,W., Krueger,M. and
            Michalski,D.
  TITLE     Impaired Neurofilament Integrity and Neuronal Morphology in
            Different Models of Focal Cerebral Ischemia and Human Stroke Tissue
  JOURNAL   Front Cell Neurosci 12, 161 (2018)
   PUBMED   29967576
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 845)
  AUTHORS   Uzasci,L., Auh,S., Cotter,R.J. and Nath,A.
  TITLE     Mass spectrometric phosphoproteome analysis of HIV-infected brain
            reveals novel phosphorylation sites and differential
            phosphorylation patterns
  JOURNAL   Proteomics Clin Appl 10 (2), 126-135 (2016)
   PUBMED   26033855
REFERENCE   3  (residues 1 to 845)
  AUTHORS   Yuan,A., Sershen,H., Veeranna, Basavarajappa,B.S., Kumar,A.,
            Hashim,A., Berg,M., Lee,J.H., Sato,Y., Rao,M.V., Mohan,P.S.,
            Dyakin,V., Julien,J.P., Lee,V.M. and Nixon,R.A.
  TITLE     Neurofilament subunits are integral components of synapses and
            modulate neurotransmission and behavior in vivo
  JOURNAL   Mol Psychiatry 20 (8), 986-994 (2015)
   PUBMED   25869803
REFERENCE   4  (residues 1 to 845)
  AUTHORS   Stegeman,S., Jolly,L.A., Premarathne,S., Gecz,J., Richards,L.J.,
            Mackay-Sim,A. and Wood,S.A.
  TITLE     Loss of Usp9x disrupts cortical architecture, hippocampal
            development and TGFbeta-mediated axonogenesis
  JOURNAL   PLoS One 8 (7), e68287 (2013)
   PUBMED   23861879
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 845)
  AUTHORS   Richard,A.F., Demignon,J., Sakakibara,I., Pujol,J., Favier,M.,
            Strochlic,L., Le Grand,F., Sgarioto,N., Guernec,A., Schmitt,A.,
            Cagnard,N., Huang,R., Legay,C., Guillet-Deniau,I. and Maire,P.
  TITLE     Genesis of muscle fiber-type diversity during mouse embryogenesis
            relies on Six1 and Six4 gene expression
  JOURNAL   Dev Biol 359 (2), 303-320 (2011)
   PUBMED   21884692
REFERENCE   6  (residues 1 to 845)
  AUTHORS   Kelly,B.M., Gillespie,C.S., Sherman,D.L. and Brophy,P.J.
  TITLE     Schwann cells of the myelin-forming phenotype express neurofilament
            protein NF-M
  JOURNAL   J Cell Biol 118 (2), 397-410 (1992)
   PUBMED   1321159
REFERENCE   7  (residues 1 to 845)
  AUTHORS   Xu,Z.S., Liu,W.S. and Willard,M.B.
  TITLE     Identification of six phosphorylation sites in the COOH-terminal
            tail region of the rat neurofilament protein M
  JOURNAL   J Biol Chem 267 (7), 4467-4471 (1992)
   PUBMED   1537832
REFERENCE   8  (residues 1 to 845)
  AUTHORS   Paz,M.M., Valente,G.B., Tasca,C.I., de Mattos,A.G. and Pureur,R.P.
  TITLE     Malnutrition induces an increase in intermediate filament protein
            content of rat cerebral cortex
  JOURNAL   J Nutr 121 (9), 1349-1354 (1991)
   PUBMED   1908892
REFERENCE   9  (residues 1 to 845)
  AUTHORS   Schlaepfer,W.W. and Bruce,J.
  TITLE     Simultaneous up-regulation of neurofilament proteins during the
            postnatal development of the rat nervous system
  JOURNAL   J Neurosci Res 25 (1), 39-49 (1990)
   PUBMED   2108255
REFERENCE   10 (residues 1 to 845)
  AUTHORS   Napolitano,E.W., Chin,S.S., Colman,D.R. and Liem,R.K.
  TITLE     Complete amino acid sequence and in vitro expression of rat NF-M,
            the middle molecular weight neurofilament protein
  JOURNAL   J Neurosci 7 (8), 2590-2599 (1987)
   PUBMED   2441012
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from Z12152.1.
            
            [WARNING] On Oct 7, 2024 this sequence was replaced by NP_058725.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z12152.1, M18628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Rattus norvegicus"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     Protein         1..845
                     /product="neurofilament medium polypeptide"
                     /note="Neurofilament protein, middle polypeptide;
                     neurofilament 3, medium; NF-M; 160 kDa neurofilament
                     protein; neurofilament triplet M protein; neurofilament,
                     medium polypeptide"
                     /calculated_mol_wt=95518
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylserine. /evidence=ECO:0000269|Ref.7;
                     propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Region          10..97
                     /region_name="Filament_head"
                     /note="Intermediate filament head (DNA binding) region;
                     pfam04732"
                     /db_xref="CDD:461414"
     Site            30
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            42
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0000250|UniProtKB:P08553; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            47
                     /site_type="glycosylation"
                     /note="O-linked (GlcNAc) threonine.
                     /evidence=ECO:0000269|PubMed:8344946. /id=CAR_000130;
                     propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Site            97
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P08553; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Region          98..409
                     /region_name="Filament"
                     /note="Intermediate filament protein; pfam00038"
                     /db_xref="CDD:459643"
     Region          103..134
                     /region_name="Coil 1A"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Region          135..147
                     /region_name="Linker 1"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Region          148..246
                     /region_name="Coil 1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Site            224
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P08553; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Region          247..263
                     /region_name="Linker 12"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Region          264..285
                     /region_name="Coil 2A"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Region          286..289
                     /region_name="Linker 2"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Region          290..410
                     /region_name="Coil 2B"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Site            318
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P08553; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            344
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Region          411..844
                     /region_name="Tail"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Site            416
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            428
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            430
                     /site_type="glycosylation"
                     /note="O-linked (GlcNAc) threonine.
                     /evidence=ECO:0000269|PubMed:8344946. /id=CAR_000131;
                     propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Site            466
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Region          482..782
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P12839.4)"
     Site            482
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            502
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:1537832,
                     ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            506
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:1537832,
                     ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            536
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:1537832,
                     ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            544
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            549
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            550
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            563
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            603
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:1537832,
                     ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            608
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:1537832,
                     ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            642
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            666
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000269|PubMed:1537832,
                     ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            686
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O77788; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            712
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            720
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            750
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     Site            766
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P12839.4)"
     CDS             1..845
                     /gene="Nefm"
                     /gene_synonym="Nef3; Nfm"
                     /coded_by="NM_017029.2:36..2573"
                     /db_xref="GeneID:24588"
                     /db_xref="RGD:3160"
ORIGIN      
        1 msytldslgn psayrrvtet pssfsrvsgs pssgfrsqsw srgspstvss sykrsalapr
       61 layssamlss aessldfsqs ssllnggsgg dyklsrsnek eqlqglndrf agyiekvhyl
      121 eqqnkeieae ihalrqkqas haqlgdaydq eirelratle mvnhekaqvq ldsdhleedi
      181 hrlkerfeee arlrddteaa iralrkdiee ssmvkveldk kvqslqdeva flrsnheeev
      241 adllaqiqas hitverkdyl ktdistalke irsqlechsd qnmhqaeewf kcryakltea
      301 aeqnkeairs akeeiaeyrr qlqsksiele svrgtkesle rqlsdieerh nhdlssyqdt
      361 iqqlenelrg tkwemarhlr eyqdllnvkm aldieiaayr kllegeetrf stfsgsitgp
      421 lythrqpsvt isskiqktkv eapklkvqhk fveeiieetk vedeksemed altviaeela
      481 asakeekeea eekeeepeve kspvkspeak eeeegekeee eegqeeeeee degvksdqae
      541 eggsekegss ekdegeqeee geteaegege eaeakeekkt egkveemaik eeikvekpek
      601 akspvpkspv eevkpkpeak agkdeqkeee kveekkevak espkeekvek keekpkdvpd
      661 kkkaespvke kaveemitit ksvkvslekd tkeekpqqqe kvkekaeeeg gseeevgdks
      721 pqeskkedia ingevegkee eeqetqekgs gqeeekgvvt ngldvspaee kkgedrsddk
      781 vvvtkkveki tseggdgatk yitksvtvtq kveeheetfe eklvstkkve kvtshaivke
      841 vtqgd
//
