LOCUS       NM_001401320            8041 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus NEDD4 like E3 ubiquitin protein ligase (Nedd4l),
            mRNA.
ACCESSION   NM_001401320 XM_039096678
VERSION     NM_001401320.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Cui,L. and Ma,J.
  TITLE     NEDD4L Promotes IkappaBalpha Ubiquitination and Degradation in the
            Pathogenesis of Diabetic Retinopathy
  JOURNAL   Curr Eye Res 49 (1), 62-72 (2024)
   PUBMED   37768316
  REMARK    GeneRIF: NEDD4L Promotes IkappaBalpha Ubiquitination and
            Degradation in the Pathogenesis of Diabetic Retinopathy.
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Liu,B.W., Zhang,J., Hong,Y.S., Li,N.B., Liu,Y., Zhang,M., Wu,W.Y.,
            Zheng,H., Lampert,A. and Zhang,X.W.
  TITLE     NGF-Induced Nav1.7 Upregulation Contributes to Chronic
            Post-surgical Pain by Activating SGK1-Dependent Nedd4-2
            Phosphorylation
  JOURNAL   Mol Neurobiol 58 (3), 964-982 (2021)
   PUBMED   33063281
  REMARK    GeneRIF: NGF-Induced Nav1.7 Upregulation Contributes to Chronic
            Post-surgical Pain by Activating SGK1-Dependent Nedd4-2
            Phosphorylation.
REFERENCE   3  (bases 1 to 8041)
  AUTHORS   Tian,R.H., Guo,K.M., Han,G.H. and Bai,Y.
  TITLE     Downregulation of MicroRNA-494 inhibits the TGF-beta1/Smads
            signaling pathway and prevents the development of hypospadias
            through upregulating Nedd4L
  JOURNAL   Exp Mol Pathol 115, 104452 (2020)
   PUBMED   32413360
REFERENCE   4  (bases 1 to 8041)
  AUTHORS   Anta,B., Martin-Rodriguez,C., Gomis-Perez,C., Calvo,L.,
            Lopez-Benito,S., Calderon-Garcia,A.A., Vicente-Garcia,C.,
            Villarroel,A. and Arevalo,J.C.
  TITLE     Ubiquitin-specific Protease 36 (USP36) Controls Neuronal Precursor
            Cell-expressed Developmentally Down-regulated 4-2 (Nedd4-2) Actions
            over the Neurotrophin Receptor TrkA and Potassium Voltage-gated
            Channels 7.2/3 (Kv7.2/3)
  JOURNAL   J Biol Chem 291 (36), 19132-19145 (2016)
   PUBMED   27445338
  REMARK    GeneRIF: USP36 actions extend beyond TrkA because the presence of
            USP36 interferes with Nedd4-2-dependent Kv7.2/3 channel regulation.
REFERENCE   5  (bases 1 to 8041)
  AUTHORS   Low,L.H., Chow,Y.L., Li,Y., Goh,C.P., Putz,U., Silke,J., Ouchi,T.,
            Howitt,J. and Tan,S.S.
  TITLE     Nedd4 family interacting protein 1 (Ndfip1) is required for
            ubiquitination and nuclear trafficking of BRCA1-associated ATM
            activator 1 (BRAT1) during the DNA damage response
  JOURNAL   J Biol Chem 290 (11), 7141-7150 (2015)
   PUBMED   25631046
REFERENCE   6  (bases 1 to 8041)
  AUTHORS   Umemura,M., Ishigami,T., Tamura,K., Sakai,M., Miyagi,Y.,
            Nagahama,K., Aoki,I., Uchino,K., Rohrwasser,A., Lalouel,J.M. and
            Umemura,S.
  TITLE     Transcriptional diversity and expression of NEDD4L gene in distal
            nephron
  JOURNAL   Biochem Biophys Res Commun 339 (4), 1129-1137 (2006)
   PUBMED   16338225
  REMARK    GeneRIF: These findings suggested that Nedd4L gene products were
            involved in the development of salt-sensitive hypertension.
REFERENCE   7  (bases 1 to 8041)
  AUTHORS   Flores,S.Y., Loffing-Cueni,D., Kamynina,E., Daidie,D., Gerbex,C.,
            Chabanel,S., Dudler,J., Loffing,J. and Staub,O.
  TITLE     Aldosterone-induced serum and glucocorticoid-induced kinase 1
            expression is accompanied by Nedd4-2 phosphorylation and increased
            Na+ transport in cortical collecting duct cells
  JOURNAL   J Am Soc Nephrol 16 (8), 2279-2287 (2005)
   PUBMED   15958725
  REMARK    GeneRIF: Sgk1-dependent phosphorylation of Nedd4-2 is part of the
            aldosterone response in kidney cortical collecting ducts
REFERENCE   8  (bases 1 to 8041)
  AUTHORS   van Bemmelen,M.X., Rougier,J.S., Gavillet,B., Apotheloz,F.,
            Daidie,D., Tateyama,M., Rivolta,I., Thomas,M.A., Kass,R.S.,
            Staub,O. and Abriel,H.
  TITLE     Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2
            mediated ubiquitination
  JOURNAL   Circ Res 95 (3), 284-291 (2004)
   PUBMED   15217910
REFERENCE   9  (bases 1 to 8041)
  AUTHORS   Harvey,K.F., Dinudom,A., Cook,D.I. and Kumar,S.
  TITLE     The Nedd4-like protein KIAA0439 is a potential regulator of the
            epithelial sodium channel
  JOURNAL   J Biol Chem 276 (11), 8597-8601 (2001)
   PUBMED   11244092
REFERENCE   10 (bases 1 to 8041)
  AUTHORS   Kamynina,E., Debonneville,C., Bens,M., Vandewalle,A. and Staub,O.
  TITLE     A novel mouse Nedd4 protein suppresses the activity of the
            epithelial Na+ channel
  JOURNAL   FASEB J 15 (1), 204-214 (2001)
   PUBMED   11149908
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On Feb 8, 2022 this sequence version replaced XM_039096678.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.35157.1,
                                           SRR26643288.59972.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760434
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on computational evidence
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-340               JAXUCZ010000018.1  60663970-60664309
            341-414             JAXUCZ010000018.1  60770848-60770921
            415-496             JAXUCZ010000018.1  60847299-60847380
            497-535             JAXUCZ010000018.1  60850304-60850342
            536-589             JAXUCZ010000018.1  60852614-60852667
            590-640             JAXUCZ010000018.1  60915014-60915064
            641-702             JAXUCZ010000018.1  60926585-60926646
            703-805             JAXUCZ010000018.1  60926973-60927075
            806-972             JAXUCZ010000018.1  60929332-60929498
            973-1108            JAXUCZ010000018.1  60937246-60937381
            1109-1285           JAXUCZ010000018.1  60938427-60938603
            1286-1360           JAXUCZ010000018.1  60940657-60940731
            1361-1420           JAXUCZ010000018.1  60942466-60942525
            1421-1552           JAXUCZ010000018.1  60946736-60946867
            1553-1672           JAXUCZ010000018.1  60947364-60947483
            1673-1870           JAXUCZ010000018.1  60948427-60948624
            1871-1948           JAXUCZ010000018.1  60953385-60953462
            1949-2003           JAXUCZ010000018.1  60955662-60955716
            2004-2062           JAXUCZ010000018.1  60960716-60960774
            2063-2128           JAXUCZ010000018.1  60966287-60966352
            2129-2358           JAXUCZ010000018.1  60968782-60969011
            2359-2480           JAXUCZ010000018.1  60970888-60971009
            2481-2551           JAXUCZ010000018.1  60973711-60973781
            2552-2647           JAXUCZ010000018.1  60976344-60976439
            2648-2721           JAXUCZ010000018.1  60982839-60982912
            2722-2782           JAXUCZ010000018.1  60985273-60985333
            2783-2842           JAXUCZ010000018.1  60987116-60987175
            2843-2950           JAXUCZ010000018.1  60987447-60987554
            2951-3047           JAXUCZ010000018.1  60988475-60988571
            3048-3120           JAXUCZ010000018.1  60989065-60989137
            3121-8041           JAXUCZ010000018.1  60991904-60996824
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..8041
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /note="NEDD4 like E3 ubiquitin protein ligase"
                     /db_xref="GeneID:291553"
                     /db_xref="RGD:735047"
     exon            1..340
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             293..3223
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /EC_number="6.3.2.-"
                     /note="neural precursor cell expressed, developmentally
                     down-regulated 4-like, E3 ubiquitin protein ligase"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase NEDD4-like"
                     /protein_id="NP_001388249.1"
                     /db_xref="GeneID:291553"
                     /db_xref="RGD:735047"
                     /translation="MATGLGEPIYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPY
                     VKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRD
                     DFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQD
                     EENSEQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRS
                     TQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASEGGGEGPEPWET
                     ISEEVNMAGDSLSLALPPPPASPVSRTSPQELSEELSRRLQITPDSNGEQFSALIQRE
                     PSSRLRSCSVTDTVAEQAHLPPPSTPTRRARSSTVTGGEESTPSVAYVHTTPGLPSGW
                     EERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLVEPQIRRPRSLSS
                     PTVTLSAPLEGAKDSPIRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRI
                     APNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTF
                     YIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLH
                     RNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYG
                     LFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMM
                     LGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEI
                     MVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGL
                     GDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGF
                     AELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGF
                     EGVD"
     exon            341..414
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            415..496
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            497..535
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..589
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            590..640
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            641..702
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            703..805
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            806..972
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1108
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1285
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1360
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1420
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1552
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1672
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1870
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..1948
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2003
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2062
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2128
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2358
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2480
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2481..2551
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2647
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2648..2721
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2782
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2842
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..2950
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3047
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..3120
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..8041
                     /gene="Nedd4l"
                     /gene_synonym="Nedd4-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggatcgccga gcgggcttgg gggaggccgg gagagccatc aaccggggcg ggagcccggc
       61 gggctcagca cagagagggg agagctccgg gccagctgcc gttcggcgcg ctctcggagc
      121 cgccagtccg cgcgtctccg cagccttccg ggaggaagcg gcgccgcggc cgggtgcagc
      181 ctcacctgcc ggcccttccc cgccgggcgg cgcagaagga cagagggtcg cggccccgcg
      241 acgcacttca gcccgtcgca gcgtctagga ccccgcgcgg gacgccggct ccatggcgac
      301 cgggctcggg gagcctatct acggactttc ggaagaggag ggagaatccc gtattctcag
      361 agtaaaagtt gtttctggga ttgacctcgc caaaaaggac atatttggag ccagtgaccc
      421 atacgtgaag ctctcgttgt acgtagctga cgagaacaga gaactcgctc tggttcagac
      481 taagacaatt aaaaagacgc tgaacccaaa gtggaatgaa gagttttatt ttagagtaaa
      541 tccatctaat cacaggctct tatttgaagt atttgacgag aacagattga cacgagacga
      601 ctttctgggc caggtggacg tgcctcttag tcaccttccg acagaagatc caaccatgga
      661 gagaccctat acatttaagg attttctcct gcgacctaga agtcataaat cacgcgtcaa
      721 ggggtttttg aggttgaaaa tggcctacat gccgaaaaac ggaggtcagg atgaagaaaa
      781 cagcgagcag agggacgaca tggagcatgg atgggaagtt gttgactcaa acgactcagc
      841 ttcccagcac caggaggagc tccctcctcc tcccctgcca ccaggatggg aagagaaagt
      901 ggacaattta ggccgaactt actatgtcaa ccacaacaac aggagtacgc agtggcaccg
      961 acctagcttg atggatgtat cgtcagagtc agacaataac atcaggcaga tcaatcagga
     1021 ggccgcacac cggcgtttcc gctctcggag gcacattagt gaagacttgg agcctgaggc
     1081 ctctgaaggt ggtggagaag gccctgagcc ttgggagacc atttcagagg aagtgaacat
     1141 ggcgggagat tctctcagcc tggctctacc cccacctcct gcctccccag tgtcaaggac
     1201 cagcccccag gagctgtcgg aagaactgag cagaaggttg cagatcactc cggactccaa
     1261 tggggaacag ttcagtgctc tgattcagag agagccctcg tctaggctcc ggtcctgcag
     1321 tgttaccgac acggttgctg aacaagctca ccttccaccg cccagcaccc caactaggcg
     1381 agcccgttcg tcaactgtca cgggtggtga ggaatcaacg ccatcagtgg cctatgtaca
     1441 taccacgccg ggcctacctt caggctggga agaaagaaaa gatgctaagg gacgcacata
     1501 ctatgtcaat cataacaatc gaaccacaac ttggactcga ccaatcatgc agcttgcaga
     1561 agacggtgcc tctggatcag ccacaaacag taacaaccac ctagtcgaac cccagatccg
     1621 ccggcctcgt agcctcagct cgccaacagt aaccttatct gccccactgg agggtgccaa
     1681 ggattcaccc atacgccgtg ctgtgaaaga taccctttcc aatccacagt cccctcagcc
     1741 atcaccttac aactccccca aaccacaaca caaagtcaca cagagcttcc tgccaccagg
     1801 ctgggagatg aggatagcgc caaacggccg acccttcttc attgaccata acacaaagac
     1861 gacaacctgg gaagatccac ggctgaaatt tccagtacat atgcggtcaa aagcatcttt
     1921 aaaccccaat gacctgggcc ctcttcctcc tggctgggaa gaaaggatcc acctggacgg
     1981 ccgcacattt tacattgacc ataatagcaa aattactcag tgggaagatc caagactaca
     2041 gaatccagcc ataactggtc cggctgtgcc gtactccaga gaatttaagc agaaatacga
     2101 ctactttagg aagaaattaa agaaacctgc tgatattcca aacaggtttg aaatgaaact
     2161 ccacagaaac aacatatttg aagaatccta tcggaggatc atgtctgtaa agagacctga
     2221 cgtcctaaag gctagactgt ggatcgagtt tgaatcagag aaaggcctgg actatggggg
     2281 cgtggccaga gagtggttct tcttactgtc caaagagatg ttcaatccct actatggcct
     2341 cttcgagtac tctgccacgg acaactacac acttcagatc aatcccaact caggcctgtg
     2401 taacgaagat cacttatcct atttcacttt cattgggaga gttgctggcc tggcagtgtt
     2461 tcatgggaaa ctcttagatg gattcttcat tcgacctttc tacaagatga tgctggggaa
     2521 gcaaataact ctgaatgaca tggagtctgt ggacagcgag tactacaact ctttgaagtg
     2581 gatcttggaa aacgacccca cggaacttga cctcatgttc tgcatagatg aagagaactt
     2641 tgggcagact taccaagtgg acctgaagcc caatgggtca gaaatcatgg taaccaatga
     2701 gaacaagcga gaatatattg acttggtcat ccagtggaga tttgtgaaca gggtccagaa
     2761 gcaaatgaat gccttcttgg agggatttac agaacttctt ccaatcgact tgattaaaat
     2821 ttttgatgaa aatgagctgg agttgctgat gtgcggcctt ggtgatgtgg acgtgaatga
     2881 ttggagacag cactccattt acaagaacgg ctactgcccc aaccaccctg tcatccagtg
     2941 gttctggaag gccgtgctgc tgatggatgc tgagaagcgg atccggttac tacagtttgt
     3001 cacaggcacc tccagagtac ccatgaatgg atttgccgaa ctttatggtt ccaatggtcc
     3061 tcagctgttt acaatagagc aatggggtag tcctgaaaaa ctgcccagag ctcatacatg
     3121 ctttaaccgc cttgatttac ctccgtatga aacctttgaa gatctacggg agaagcttct
     3181 catggccgtg gagaatgctc aaggcttcga aggtgtggat taaggaaggc ctcggcgtca
     3241 gggcaccgcc cttccagcaa ggtccgcgtg cacttttgca tttgcctagc agacttttgc
     3301 agagcccgtg gcagaagcag ttgcctggcc tggctctgga gcccagcttg tccgggccct
     3361 gcccgagttc cgccagggaa cccagccccg ctcaagtgcc gccccctcac cgccgataac
     3421 tcaactggtt gatgtgtaca ctgattacag gaggacttgt tctatttatg ttgtgcctct
     3481 gcaggcaaag cccttaataa atattttgca tactctctaa tgacagtgaa tggaattaat
     3541 cacgctccag gtatagtatt acaactcatg tttacttttt aaaaatgatt tagactgatt
     3601 ttcagatttt atttcattac aattaaagat gtctcatgta cttggaaaag tgagcatatt
     3661 ttttttgtat ttgaatttca tatcaagctt aatgttggtg acattttttt tttatttttg
     3721 aagtactttg acactcccag cctccacttt gttagaattg gaagactaat ttttctccaa
     3781 aagccttcta cagacaaata ctttgaaaga acttcaacta taacattgag cataatattt
     3841 tttccatact cagattgaaa ataaactgga tattaccttt tttttttttg tacaaattta
     3901 gagactaggt ccaggctgga agaattgtaa catagcatga tatctttgtg ttaacttgag
     3961 aggaatgacc ccactgtccc ttagaagttc tgacgtgtgc catcatgcat ttaaagacag
     4021 gactggactg tcatcttgca tcagaaacca ttacagtttc ctgtcgctgt acagcctcct
     4081 ctgaactctc ttttctcttt ggtgttcata gagacatttc caatggcatt gctccattca
     4141 aaaccaagga aaccagaatc actgatcttc tgtctctgat gattgctaaa caataatcaa
     4201 ataacccttc tttgctgtat cctcgtccct tctgaaggca ctttacggag gtcacatccg
     4261 gttttgtttt cattttgttc tcacggatat cacgcgtggc gattgttcct tcctccctgc
     4321 acacatctgt ccggtgcaga tctatcagtc gtgaacttgg cctcgagtat acatatgagc
     4381 ccagatgtga cgcccagcct ctgcaccgat cttctccgac tgttctgtaa tttctactca
     4441 acaacaaggt tttacctact gtacacttaa ctgagttgtg tgacctatga cctactctgc
     4501 atgctccgat accgtgtacc gcccccgggt catgtctatg gtgtgctagt tttaaaggtg
     4561 acctggaaag cctagtggga accgctgagc ccaagccatc tggctggcag cggtggaagc
     4621 gagagagggt gtctgaagtt ttctaagtct ctttctgttg tgttctgaga agatgcctga
     4681 ggcagcgtgg gagttccgac acccctgctg tccctttaac ttgtgtcgtc ctagaccctg
     4741 tcctagcagt aagggggaca tgggatattt ctgatctctt gccaagttgt gaataagacg
     4801 aaaagactgt ggtccctgcc acataagaag gatctttggt ataagaaatc gctcgtgttt
     4861 acacagctgt ggatgccgtg aaggaaccat ggacattctt ggtgggtgaa gccctagacg
     4921 gtggaatcct accaagctgg ccatcggctt cgatccacat gctatcaagt aattacagag
     4981 gcaattaaca ctcactgttc ctgcctccgc tgggataatt tccttctgta gtaaacagat
     5041 attgggcaga gggggtgggg ggggatattt tttcttaata accttccagg tccagaacca
     5101 tctctgttat agagggtgcc aaagcctgag ctaaactgta ttttaactgg agaagaaacc
     5161 gaaatctatt ctaagagctg gggttctata ctcccgtagg ccctataccg aacaacacga
     5221 ttcacttaaa tgcaaagtag tcgggtatta ggaaatgtaa aaggagccag caagatggct
     5281 cagtgagttg aggtcaccaa tcctgacatc ctgagttcaa tccctggaac ccacctgaca
     5341 ggagagaact gacttctaca agttgtcctc tgatataaca attttactaa aaaaaatata
     5401 ataataatta aaaatatgaa aagaggactt cattctgatc atttctaagt caagagcaga
     5461 gaagacctat taaaataacc ctccagggaa tacgtgaaag gcaagccatt gtgaatgaca
     5521 cttcggttca ttggcccgag ataatttcag ggtttaacag tggttactgt tactggagat
     5581 gggcgagctg gcctgttaga gagtaaagat agagtagttt cctgtccctc tggtttgtga
     5641 cgttccactt gttgcccgaa atgcccgatg cctcgtctcc agaccccgct ggccatggca
     5701 ggtcattctc cgatctccct actgcagctg gtgctgttcc cctctgtgct gtggagatga
     5761 catcatccat ctccctgcaa acagtaatgg tggatctttt accttgaggc tgcgtgcgga
     5821 gtgggtccca gaactctgac tttaccatgg aaggactttg agcatcctgt ggctctctta
     5881 tgtccctgcc cctttgactc cttggtggac ctgggattag acagtctgct gactccagaa
     5941 caggtcgtgg taggcgttag tctatcctag gtgccctcct gtgctgtcct ggtctgtctg
     6001 aaacacacat ctctactttt ctgtggccag tcctctgaca gttcacaaga accagacacg
     6061 tgtactttta tttttgtttc caggactgat gtagcttagt ggcaagcgtt tgcctagctg
     6121 tagattcaat ccctgttgct ggggcccggg gagggggtgg ataagaaata gaaaatttct
     6181 ctttccgagg acctttagca agagacagga aatcctgaca ttcatgttgg aaattatata
     6241 aaccaacatg acaagaaccg tttcccccca aaaggctcag cagccgactc ttgggagtga
     6301 gatacaaaat cttttaacca cttttgaatc tcacttggcc tgtggctctt ggtcttgcta
     6361 gattccagtt tgttctgaga gtcaggctct cacgtacagc agcttctaac ctgttctcta
     6421 tttccctggt agtctccttt ctcacctctg aaatttgaaa gtcttctttt cttcttatct
     6481 tcctcttcct ccttctctct ctcctttctc tcttccctct ctcctctcct ctcctctcct
     6541 ctctctctct ccgtccccct ccctcccctt ctctctctct ccccctctct ctgcttctct
     6601 ctcaacaaca gaagggagat gctatctgca accattctct tcctggccag taggggcgct
     6661 gctgtgcagg gtcactgcct gccctgctcc ccctgtgact cccagaggac cacacgctac
     6721 ctctgtctca ttctcaaatt ggggtctatc ctaagtcatg tcaaactgcc gatttcagga
     6781 ctgggatgcg ttttaaaaca aaaaaaacaa aaaaccctaa actccacttt gtaccttaga
     6841 tttttaaaac tgggtagaaa tgtaatatac attggaccac tttttttttt aatttctata
     6901 agccttaatg aacagtgtat agacatctgt gtatgcgcac tctgccccag agtgttttca
     6961 gtgtgccaga ggagaagccg tagtgtgagg acatgtctgt ctagtgtttc ctaggaaaag
     7021 ggtagctttc ttttggtgta aaatttccta cggaaaccac tcagcatcct ttgttagaga
     7081 tgttgacgac gctccaagca cgtgtctgag gcacatgtat ctttaccggt ttcatatttg
     7141 taagaggcaa tggtaggaac ctaagagcat ctgtcagtgt gtgacttcta atgccctgca
     7201 ttccctccgc tgtgtctcag tgtttgtatt gaaagcctct cctgtgccca cagcctgtgt
     7261 tgacagatgt ctgtccctgt gcaagcctca gactcagact gatgtatcct acgttgtcgg
     7321 aattttcttc ctgttatctc tgtgactgtt cttccttttg ctacccagga tgtaacatat
     7381 atacatatat gtgtgtgtgt atgtgtgtgt gtgctatcca aatatatttg tatatatttg
     7441 tatagcattt ttacacttta ggagtaatct ggaattcaaa agggaatgag agctgactcc
     7501 cttccacagt tgtctccaag tcctgccctg agaattctga ccctcggcgt ctgagagaac
     7561 cctaggagga cattattaac ttgctgtcat tttcttaatt cagaatctgt taatcaaatg
     7621 agacattttt caaaagtgac tctgaaggtg aaaagagttt caagcattcc aaatgaatcc
     7681 tccatgtttc ttaacttttt attacaatcc ccccacccac acccaccttc tcacacaaag
     7741 ctgagtgcta tttgatacaa tggtcaaaac caaactacct tgacgttttt ctaagacagc
     7801 tcaatggctg acgctctttt tcacagtcct cgagctttaa cctagctcga acctggtaca
     7861 cacacgtaaa gttttaaggt cgagtcctct tctgtcttct tgagagacct ggagagatac
     7921 tgtagcttgt tttcctgaca gcaaatggga actgtgacaa agtgaagtcg ttgtaaaggg
     7981 gtgatgcaac ttgtcaaata tttaataaag aattatggaa gccggaacat ttcctacaca
     8041 a
//
