LOCUS       NM_001029919            2459 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus NECAP endocytosis associated 1 (Necap1), mRNA.
ACCESSION   NM_001029919
VERSION     NM_001029919.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ritter,B., Murphy,S., Dokainish,H., Girard,M., Gudheti,M.V.,
            Kozlov,G., Halin,M., Philie,J., Jorgensen,E.M., Gehring,K. and
            McPherson,P.S.
  TITLE     NECAP 1 regulates AP-2 interactions to control vesicle size,
            number, and cargo during clathrin-mediated endocytosis
  JOURNAL   PLoS Biol 11 (10), e1001670 (2013)
   PUBMED   24130457
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Ritter,B., Denisov,A.Y., Philie,J., Allaire,P.D.,
            Legendre-Guillemin,V., Zylbergold,P., Gehring,K. and McPherson,P.S.
  TITLE     The NECAP PHear domain increases clathrin accessory protein binding
            potential
  JOURNAL   EMBO J 26 (18), 4066-4077 (2007)
   PUBMED   17762867
REFERENCE   3  (bases 1 to 2459)
  AUTHORS   Murshid,A., Srivastava,A., Kumar,R. and Presley,J.F.
  TITLE     Characterization of the localization and function of NECAP 1 in
            neurons
  JOURNAL   J Neurochem 98 (6), 1746-1762 (2006)
   PUBMED   16879712
  REMARK    GeneRIF: NECAP 1 plays a role in clathrin-mediated neuronal
            endocytosis, including a role in presynaptic endocytosis. (NECAP 1)
REFERENCE   4  (bases 1 to 2459)
  AUTHORS   Mishra,S.K., Hawryluk,M.J., Brett,T.J., Keyel,P.A., Dupin,A.L.,
            Jha,A., Heuser,J.E., Fremont,D.H. and Traub,L.M.
  TITLE     Dual engagement regulation of protein interactions with the AP-2
            adaptor alpha appendage
  JOURNAL   J Biol Chem 279 (44), 46191-46203 (2004)
   PUBMED   15292237
REFERENCE   5  (bases 1 to 2459)
  AUTHORS   Ritter,B., Philie,J., Girard,M., Tung,E.C., Blondeau,F. and
            McPherson,P.S.
  TITLE     Identification of a family of endocytic proteins that define a new
            alpha-adaptin ear-binding motif
  JOURNAL   EMBO Rep 4 (11), 1089-1095 (2003)
   PUBMED   14555962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC097496.1 and CO402572.1.
            
            On Oct 20, 2007 this sequence version replaced NM_001029919.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC097496.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1019              BC097496.1         1-1019
            1020-1592           CO402572.1         137-709
            1593-2459           BC097496.1         1592-2458
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..2459
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /note="NECAP endocytosis associated 1"
                     /db_xref="GeneID:312694"
                     /db_xref="RGD:1306053"
     exon            1..114
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..853
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /note="adaptin ear-binding clathrin-associated protein;
                     NECAP-1; NECAP endocytosis-associated protein 1"
                     /codon_start=1
                     /product="adaptin ear-binding coat-associated protein 1"
                     /protein_id="NP_001025090.1"
                     /db_xref="GeneID:312694"
                     /db_xref="RGD:1306053"
                     /translation="MAAELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWT
                     GRLRITSKGKIAYIKLEDKVSGELFAQAPVEQYPGIAVETVADSSRYFVIRIQDGTGR
                     SAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISKESQEMDSRPKLDLGFKEGQTIK
                     LSIGNITAKKGGTSKPRASGTGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKS
                     NHGSNDSDILLDLDSPAPVPTSAPAPAPASTSNDLWGDFSTASSSVPNQAPQPSNWVQ
                     F"
     exon            115..215
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     exon            216..320
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     exon            321..402
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     exon            403..511
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     exon            512..695
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     exon            696..804
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
     exon            805..2419
                     /gene="Necap1"
                     /gene_synonym="RGD1306053"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggcgctagc ggagccaaga tggcggcgga gctggaatac gagtctgtgc tgtgtgtgaa
       61 gcccgacgtc agcgtctacc ggattccgcc gcgggcctcc aaccgcggtt acagggcatc
      121 tgactggaag ctagaccagc ctgattggac tggtcgcctc cgaatcactt caaaagggaa
      181 gattgcctac atcaaactgg aagataaagt ttcaggggag ctcttcgctc aggcgccagt
      241 agagcagtac cctgggattg ctgtggagac tgtggccgac tccagccgct actttgtgat
      301 caggatccag gatggcaccg ggcgcagtgc gtttattggc atcggcttca cggaccgggg
      361 agatgccttc gactttaatg tctccctgca agatcacttc aagtgggtaa agcaggaaac
      421 cgagatctcc aaagaatcgc aggaaatgga tagtcgtccc aagttggatt taggcttcaa
      481 ggaagggcaa accatcaagc tgagtattgg gaacattaca gccaagaaag ggggtacttc
      541 taagccccgg gcctcaggaa cggggggcct gagcttactc ccacctcctc ctggaggcaa
      601 agtcactatc cccccaccgt cctcctccgt tgccatcagc aaccacgtca ccccaccacc
      661 cattccaaaa tctaaccatg gaagtaatga ttcagatatc ctgttagatt tggattctcc
      721 agctcctgtc ccgacttcag caccagctcc agctccagct tctacaagca atgacttgtg
      781 gggagacttt agcactgcat ccagctctgt tccaaaccag gcaccacagc catctaactg
      841 ggtccagttt tgagtcgcat tggcaagaag ttgaggacac ttgaagaata aaaatgacct
      901 caagggcacc attctatgag ggagttgagg gacggcttaa tttcccagga cccaaatcag
      961 tggtcagtct ttcctgtagc ttctctgtgc attcaggctg gatttttttt tttttttttt
     1021 ggttacctct gtgttacttg ctgtatatcc aggagacaat ctgctgtttc ctgctcagaa
     1081 ccaagcaagg gagtagtggg tattatcaca ctgactgact ttgcagagtt cagaaggcca
     1141 acttgatgag tgggagtgac ctcgaacgta tgtaaatcct tgaacttatt tcagaatcat
     1201 ctcatgattc cctagttagc aatttcagga gagacaaatg ccttgaaact gtcttctcca
     1261 ctaatccgag actaaatatg gtcaggctgg ccccaggact catgaagtta gggttttcat
     1321 gggggtagat ttggagaaag ctgtgtcccg gctctcttct gtaaggcctc cttcaggctt
     1381 accccatgca gtgaacttcc cgtgctgggt ggagccccat caccttcttg tgtgtttaca
     1441 tgttgtttcc tttgacaaga gggttatgtt ggtggcacct cactgttttc ttgttgaata
     1501 gtgcagcatc tttgaccagt gaatatttct gagatgaagg ggtcaagggg ctgtgctttc
     1561 catggtgtag tctacagaag tgtttaattt cttgcggccc cacgggattg ctgcactgac
     1621 gcatagaatt gatctatact caccctgtgt ttgacctgaa gagttttaac ttgatgtgta
     1681 gagcagagag ctggaagcac taagttccca ttcagtaccc acaatgcctt gctgcctggt
     1741 ttgactcctt ttcataaaca tttcatttca gtccatctag cacttctgtg gaaagctgct
     1801 gttgattgtg tcagtgtgaa ggaggtgaag tcacagcttt ctttacctat gacagttagg
     1861 ctttgcacta gacgttgata ccagctagga tatcttaaag gaagttaccg ccccatcact
     1921 ctccagtctc tggccgccat tccttttaca gtgctgtgaa gagcgtcctc tgaggtcggt
     1981 gggtactgtc tcctgttggt cgggcagttt gagggaggag tgggaggact cacactcctg
     2041 caggtacctg tttgggtagc acactggctg cagagagtcc tttcagatat attgtttctc
     2101 aatgttcttc gtagcttttt ctaacttcgg gtccattttt cccatcgcct cttcccattc
     2161 ccaggcagct ctcttgttgc agagccatgg caggacgttt aagttccaat aaaaacacta
     2221 agaagaaagt atagaatcac tagtgactgt tgggaaacct attttctcaa tcttcctcca
     2281 ttttgtgttc tttgtattct taagatgata atatattatg tatttgaatt gctgaaaatt
     2341 gaaaatgaag ttgaagatat atgtatataa gcgtatgctg tattggtgca ataatggtaa
     2401 ttaaagatat taaaaaagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
