LOCUS       NM_133415               1575 bp    mRNA    linear   ROD 08-APR-2024
DEFINITION  Rattus norvegicus N-terminal EF-hand calcium binding protein 2
            (Necab2), mRNA.
ACCESSION   NM_133415
VERSION     NM_133415.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,Y., Deng,Q., Li,S., Chen,M., Jin,B. and Wang,M.
  TITLE     TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by
            Interacting with NECAB2
  JOURNAL   J Mol Neurosci 71 (1), 55-65 (2021)
   PUBMED   32557241
  REMARK    GeneRIF: TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by
            Interacting with NECAB2.
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Yachie,N., Petsalaki,E., Mellor,J.C., Weile,J., Jacob,Y., Verby,M.,
            Ozturk,S.B., Li,S., Cote,A.G., Mosca,R., Knapp,J.J., Ko,M., Yu,A.,
            Gebbia,M., Sahni,N., Yi,S., Tyagi,T., Sheykhkarimli,D., Roth,J.F.,
            Wong,C., Musa,L., Snider,J., Liu,Y.C., Yu,H., Braun,P.,
            Stagljar,I., Hao,T., Calderwood,M.A., Pelletier,L., Aloy,P.,
            Hill,D.E., Vidal,M. and Roth,F.P.
  TITLE     Pooled-matrix protein interaction screens using Barcode Fusion
            Genetics
  JOURNAL   Mol Syst Biol 12 (4), 863 (2016)
   PUBMED   27107012
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1575)
  AUTHORS   Canela,L., Fernandez-Duenas,V., Albergaria,C., Watanabe,M.,
            Lluis,C., Mallol,J., Canela,E.I., Franco,R., Lujan,R. and
            Ciruela,F.
  TITLE     The association of metabotropic glutamate receptor type 5 with the
            neuronal Ca2+-binding protein 2 modulates receptor function
  JOURNAL   J Neurochem 111 (2), 555-567 (2009)
   PUBMED   19694902
  REMARK    GeneRIF: NECAB2 by its physical interaction with mGlu(5b) receptor
            modulates receptor function
REFERENCE   4  (bases 1 to 1575)
  AUTHORS   Canela,L., Lujan,R., Lluis,C., Burgueno,J., Mallol,J., Canela,E.I.,
            Franco,R. and Ciruela,F.
  TITLE     The neuronal Ca(2+) -binding protein 2 (NECAB2) interacts with the
            adenosine A(2A) receptor and modulates the cell surface expression
            and function of the receptor
  JOURNAL   Mol Cell Neurosci 36 (1), 1-12 (2007)
   PUBMED   17689978
REFERENCE   5  (bases 1 to 1575)
  AUTHORS   Sugita,S., Ho,A. and Sudhof,T.C.
  TITLE     NECABs: a family of neuronal Ca(2+)-binding proteins with an
            unusual domain structure and a restricted expression pattern
  JOURNAL   Neuroscience 112 (1), 51-63 (2002)
   PUBMED   12044471
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Nov 26, 2020 this sequence version replaced NM_133415.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF193757.1, SRR26643298.76668.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-161               JAXUCZ010000019.1  64409999-64410159
            162-186             JAXUCZ010000019.1  64412935-64412959
            187-295             JAXUCZ010000019.1  64414968-64415076
            296-321             JAXUCZ010000019.1  64417078-64417103
            322-419             JAXUCZ010000019.1  64417234-64417331
            420-556             JAXUCZ010000019.1  64426975-64427111
            557-675             JAXUCZ010000019.1  64429566-64429684
            676-755             JAXUCZ010000019.1  64429813-64429892
            756-809             JAXUCZ010000019.1  64432134-64432187
            810-922             JAXUCZ010000019.1  64432646-64432758
            923-1000            JAXUCZ010000019.1  64434579-64434656
            1001-1092           JAXUCZ010000019.1  64435437-64435528
            1093-1575           JAXUCZ010000019.1  64435886-64436368
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..1575
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /note="N-terminal EF-hand calcium binding protein 2"
                     /db_xref="GeneID:170928"
                     /db_xref="RGD:621844"
     exon            1..161
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1121
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /note="neuronal calcium-binding protein 2; EF hand calcium
                     binding protein 2; neuronal calcium binding 2"
                     /codon_start=1
                     /product="N-terminal EF-hand calcium-binding protein 2"
                     /protein_id="NP_596906.2"
                     /db_xref="GeneID:170928"
                     /db_xref="RGD:621844"
                     /translation="MGEPRAPLVPDIPAADPDPGPAAPRGGTAVILDIFRRADKNDDG
                     KLSLEEFQLFFADGVLNEKELEGLFHTIDSDNTNHVDTKELCDYFVEHMGDYEDVLAS
                     LETLNHSVLKAMGYTKKVYEGGNNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQ
                     TSQIRQDHCKPSPGVNESRYGGPTPPYIPNHKLVVPEPVKSLPVAIGEPKEEGLEVQI
                     SRLAELIGRLESKTLSFDLQQRLSDEEGTNMYLQLVRQEMAVCPEQLGEFLDSLRQYL
                     RSTAEERNCFHVAAVRMADGLTFVIYEFWETEEEWKRHLQSPVCKAFRHVKVDTLSQP
                     EALSQISVPAAWCTSGRD"
     exon            162..186
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            187..295
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            296..321
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            322..419
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            420..556
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            557..675
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            676..755
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            756..809
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..922
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1000
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1092
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1575
                     /gene="Necab2"
                     /gene_synonym="Efcbp2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tccgggagcc gccgccgcag ggccgcgcgc tgggcgggct gctgcgctgg gtgggcgcca
       61 ggatggggga accccgcgca ccgctcgtcc ccgacatccc cgccgcggac cccgaccccg
      121 ggcccgccgc accgcgaggg ggcacagccg tcatcctgga cattttccgc cgtgcggata
      181 aaaacgacga cgggaaactg tcccttgagg aatttcaact gttcttcgcg gatggcgtcc
      241 tcaatgagaa ggagctggag ggcctcttcc acaccattga ttcagacaac accaaccatg
      301 tggacacaaa ggaattgtgt gattactttg tggaacacat gggagactac gaagatgtcc
      361 tggcctccct cgagactttg aaccactcag tcctgaaggc tatgggctac accaagaagg
      421 tgtatgaagg tgggaacaac gtggaccagt ttgtgacccg cttccttctg aaggagacgg
      481 ccaatcagat ccagtcgctg ctcagctctg tggaaagcgc cgtggaagcc atcgaagaac
      541 agaccagcca gatccgacag gaccactgca aacctagtcc tggcgtcaac gagagccgct
      601 acggaggacc cactcctccc tacatcccca accacaagct tgtggtcccg gaaccagtga
      661 agagcctgcc tgtggccata ggggagccaa aggaggaggg gctggaggtc cagataagcc
      721 ggttggcaga gctgattggg agactggaga gcaagaccct ctcgtttgac ctgcagcagc
      781 gcctctcgga tgaagaaggc accaacatgt acctgcagct ggtccggcag gagatggccg
      841 tgtgccctga gcagctgggc gagttcttgg attccctgag acagtacctt cggagcactg
      901 ctgaagaaag gaactgcttc catgtggccg cggtgaggat ggcagatggg ctaaccttcg
      961 tcatctacga gttctgggag acagaggagg agtggaagag gcacctgcag agccctgtgt
     1021 gcaaggcctt ccgacatgtc aaggttgaca cactgagcca gcctgaggca ctctcccaga
     1081 tctccgtgcc agctgcctgg tgcacctccg gccgagactg acccctccca gttaagaagt
     1141 cctactgcta gtcccatgac ccttcttgta aaggccaata cccttttcta gatactttgg
     1201 taaacagctg tcttttcaat atacgtatat atcaaaggac ttgtatttcc cctgctgtgg
     1261 cacagaggag gcccatcccc tggcgtcccc acacctgact ccacctggca gactactctt
     1321 gcaacgatcc tggcacgcta gtaggggtgg cttccccacc ccagccagct tctggcatcc
     1381 ctgattctta atagctgagg cccagcaggg cccacctcag gtggacaggc ctgctcctct
     1441 acatctgtcc tctttgatgg ccctctacct agtaggagct gtgaccctgt agtacccaat
     1501 ctaggcttgg gtagagccca ggtccccagc accacactag gcccacagcc accaaataaa
     1561 gtctggttag gaaca
//
